Analusis最新文献_第8页

■ Nitrogen monoxide and oxidative stress: composition and intensity of cellular oxidative bursts cocktail. A study through artificial electrochemical synapses on single human fibroblasts ■一氧化氮和氧化应激:细胞氧化爆发鸡尾酒的成分和强度。利用人工电化学突触对单个人成纤维细胞的研究

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000280506

C. Amatore, S. Arbault, D. Bruce, P. Oliveira, M. Erard, N. Sojic, M. Vuillaume

{"title":"■ Nitrogen monoxide and oxidative stress: composition and intensity of cellular oxidative bursts cocktail. A study through artificial electrochemical synapses on single human fibroblasts","authors":"C. Amatore, S. Arbault, D. Bruce, P. Oliveira, M. Erard, N. Sojic, M. Vuillaume","doi":"10.1051/ANALUSIS:2000280506","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280506","url":null,"abstract":"Positioning an ultramicroelectrode at micrometric distances from an isolated living cell ensures tgha any electroactive material released by the cell is collected and analyzed by the electrode surface. The film of extracellular fluid comprised between the cell and the electrode surfaces defines an artificial synaptic cleft of a few hundred femtoliters volume, in which the release of minute molecular amounts of chemicals produces a sudden and important concentration rise. This guarantees the detection of the released species with an extremely high signal-to-noise ratio, as well as a determination of its instant released flux since the collection efficiency is quantitative. In other words, the assembly cell/liquid cleft/ultramicroelectrode behaves as an artificial neuronal synapse. In this review we wish to elaborate on how this artificial synaptic arrangement may be used to monitor an oxidative stress response stimulated in a human fibroblast, and demonstrate that such long-time conjectured oxidative cellular bursts involve a subtle cocktail of femtomoles of hydrogen peroxide, nitrogen monoxide, peroxynitrite and nitrite ions. The analysis performed establishes that this delicate cocktail results from the ultimate combinations of equimolar primary productions of superoxide ion and nitrogen monoxide by two distinct enzymatic systems which are presumably operating in distinct compartments of the cell: NADPH-oxidase type enzymes for the generation of superoxide, and NO-synthases for that of nitrogen monoxide.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"24 1","pages":"506-517"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89145612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Nitrogen monoxide and oxidative stress: composition and intensity of cellular oxidative bursts cocktail. A study through artificial electrochemical synapses on single human fibroblasts 一氧化氮和氧化应激:细胞氧化爆发鸡尾酒的成分和强度。利用人工电化学突触对单个人成纤维细胞的研究

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000280518

M. Trujillo, M. Naviliat, M. Álvarez, G. Peluffo, R. Radi

{"title":"Nitrogen monoxide and oxidative stress: composition and intensity of cellular oxidative bursts cocktail. A study through artificial electrochemical synapses on single human fibroblasts","authors":"M. Trujillo, M. Naviliat, M. Álvarez, G. Peluffo, R. Radi","doi":"10.1051/ANALUSIS:2000280518","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280518","url":null,"abstract":"Peroxynitrite is a powerful and cytotoxic oxidant formed by the diffusion-limited reaction between . NO and O 2 - . Peroxynitrite reactions, including those with thiols, transition metal centers and CO 2 , that account for more than 95% peroxynitrite reactivity in biology, and b) the hydroxyl radical/nitrogen dioxide pathway, which arises from homolysis of peroxynitrous acid. Different methods have been utilized to expose biochemical or cellular systems to peroxynitrite including a) addition of authentic peroxynitrite, either as a bolus or a continuous infusion, and b) generation of fluxes of the radical precursors . NO and O 2 - . Reaction yields and product distributions are sometimes difficult to interpret, mainly due to the multiple possible and competing radical interactions. Peroxynitrite detection relies on a) the detection of footprints, and b) the modification of probes. The lack of total specificity of the methods in use makes obligatory the use of a combination of them and/or pharmacological approaches in order to unambiguously unravel peroxynitrite formation either in biochemical or biological systems.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"5 1","pages":"518-527"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79220043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Determination of nonionic polyethoxylate surfactants in wastewater and sludge samples of sewage treatment plants by liquid chromatography-mass spectrometry 液相色谱-质谱法测定污水处理厂废水和污泥样品中的非离子聚氧乙酸表面活性剂

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000167

S. Chiron, E. Sauvard, R. Jeannot

{"title":"Determination of nonionic polyethoxylate surfactants in wastewater and sludge samples of sewage treatment plants by liquid chromatography-mass spectrometry","authors":"S. Chiron, E. Sauvard, R. Jeannot","doi":"10.1051/ANALUSIS:2000167","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000167","url":null,"abstract":"An analytical method is proposed in order to determine the major nonionic surfactants, octylphenolpolyethoxylates (OPEOs), nonylphenolpolyethoxylates (NPEOs) and aliphatic alcoholpolyethoxylates (AEOs), and their metabolites, nonylphenol and octylphenol in wastewater and sludge samples of sewage treatment plants. This method involved a Soxtec extraction step and a solid phase extraction step for the nonionic surfactant extraction from sludge and wastewater samples, respectively. Wit h both methodologies, all recovery values were higher than 70 %. Quantitation and identification of individual compound were car- ried out by liquid chromatography-mass spectrometry (LC/MS) using electrospray (ESP) as ionization mode. Reverse phase LC chromatography allowed the elution of all oligomer constituents of each homolog component into a single peak. Gathering all the oligomers into a single peak increased the peak intensity and provided a way to determine low concentration of APEOs and AEOs in different environmental matrices. Information on the oligomers distribution of APEOs and AEOs could be obtained by the extraction of selected m/z ions from the TIC chromatograms. Positive ESP LC/MS analyses of AEOs and APEOs yielded pri- mary pseudo-molecular ions of the (M + Na)+ type for each oligomer. The negative ionization mode allowed the determination of the two metabolites, nonylphenol and octylphenol, characterized by the pseudo molecular (M-H) - ion. Limits of detection achieved were 0.1 μg/L in wastewater sample and 10 ng/g in sludge samples. The developed LC-ESP/MS method was applied to the analysis of wastewater and sludge samples of two sewage treatment plants located in Orleans (France).","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"50 1","pages":"535-542"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77780068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Analytical artifacts caused by the presence of water vapor in the headspace of food products 由于食品顶部空间中存在水蒸气而引起的分析伪影

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000170

D. Canac-Arteaga, C. Viallon, J. Berdagué

{"title":"Analytical artifacts caused by the presence of water vapor in the headspace of food products","authors":"D. Canac-Arteaga, C. Viallon, J. Berdagué","doi":"10.1051/ANALUSIS:2000170","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000170","url":null,"abstract":"The aim of the work reported here was to underscore major artifacts appearing during the purge-and-trap step of the volatile fraction analysis of water-rich products. For this purpose we humidified the headspace of dry and lowmoisture products to simulate the artifacts produced during the analysis of naturally moist products. This method implemented with and without dry purge of the trapping adsorbent also enabled us to determine the origin of the observed interference. Materials and methods Samples Two food products of animal origin, namely dehydrated beef stock and powdered Parmesan cheese, were selected for their low water content (respectively 2.3 and 25.0 %) and for the diversity of the chemical families present in their volatile fractions. Description of experiments carried out To target the origin of the water-related artifacts, different analyses were carried out with and without humidification of the headspace of the products, and with and without a dry purge step. In all, eight analyses were performed: two products (dehydrated beef stock and Parmesan cheese) in two moisture states (dry and moist) with two drying states for the Tenax trap (with and without dry purge).","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"30 1","pages":"550-556"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89165599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Measurement of nitric oxide, nitrite and nitrate using a chemiluminescence assay:an update for the year 2000 用化学发光法测定一氧化氮、亚硝酸盐和硝酸盐:2000年最新情况

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000280455

M. S. McMurtry, D. H. Kim, T. Dinh-Xuan, S. Archer

{"title":"Measurement of nitric oxide, nitrite and nitrate using a chemiluminescence assay:an update for the year 2000","authors":"M. S. McMurtry, D. H. Kim, T. Dinh-Xuan, S. Archer","doi":"10.1051/ANALUSIS:2000280455","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280455","url":null,"abstract":"This review deals with the measurement of nitric oxide (NO) using the chemiluminescence assay. A preliminary discussion is offered to explain the chemical basis for this assay and emphasize the importance of measuring NO. After reviewing some practical aspects and caveats of the chemiluminescence assay, we review its application in a variety of research and clinical settings, such as measurement of breath NO and serum nitrates. The importance of avoiding confounding effects of dietary nitrates when assessing the NO system in humans is discussed and new data are provided on the confounding effects of atmospheric NO on measurement of breath NO. The utility and ease of the chemiluminescence assay for these applications is contrasted with the challenge of using chemiluminescence for correlation of vascular tone and NO production, studies in which real-time measurements are required. Although the chemiluminescence assay is one of the most reliable, rapid and reproducible assays available, it is optimal for gas phase measurements and may be suboptimal for measurement under certain circumstances. This chapter complements several more general reviews of the methodology for measuring NO.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"10 1","pages":"455-465"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83281977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

■ Detection of superoxide anion release by endothelial cells using reduced cytochrome c UV-visible absorption, ESR spin trapping, hydroethidine fluorescence and lucigenin-enhanced chemiluminescence techniques ■利用还原性细胞色素c紫外可见吸收、ESR自旋捕获、氢乙二胺荧光和荧光素增强化学发光技术检测内皮细胞超氧阴离子释放

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000280475

J. Souchard, M. Barbacanne, F. Bayard, F. Nepveu, J. Arnal

{"title":"■ Detection of superoxide anion release by endothelial cells using reduced cytochrome c UV-visible absorption, ESR spin trapping, hydroethidine fluorescence and lucigenin-enhanced chemiluminescence techniques","authors":"J. Souchard, M. Barbacanne, F. Bayard, F. Nepveu, J. Arnal","doi":"10.1051/ANALUSIS:2000280475","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280475","url":null,"abstract":"Reduced cytochrome c UV-visible absorption, ESR spin trapping, hydroethidine fluorescence and lucigenin-enhanced chemiluminescence techniques, were used to study O 2 - production from A23187-stimulated bovine aortic endothelial cells (BAEC). All detected O 2 - release but some lack of sensitivity and specificity in probes and enzymes occured. Cytochrome c prepared with trichloroacetic acid (TCA), TCA-free or acetylated (C2506, C7752, C4186) provided similar superoxide-dismutase (SOD)-inhibitable absorbances at 20 μM. At a higher concentration (100 μM) TCA-prepared cytochrome c could no longer detect O 2 - production whereas TCA-free cytochrome c detected less O 2 - at 100 μM than at 20 μM. Catalase C-40 was not contaminated with SOD-like impurities. Lucigenin provided high artefactual amounts of O 2 - production from stimulated BAEC in the presence of NADH or NADPH. Moreover, even at a low concentration (5μM) lucigenin and NADH (or NADPH) decreased endothelium-dependent relaxation induced by acetylcholine to 50 %. The use of lucigenin is not recommended.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"3 1","pages":"475-478"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85602527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Contribution of spin-trapping EPR techniques for the measurement of NO production in biological systems 自旋捕获EPR技术对测量生物系统中NO生成的贡献

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000280445

Y. Henry, A. Guissani

引用次数: 14

Polycyclic aromatic hydrocarbons in highway plants and soils. Evidence for a local distillation effect 公路植物和土壤中的多环芳烃。局部蒸馏效应的证据

Analusis Pub Date : 2000-06-01 DOI: 10.1051/ANALUSIS:2000280290

C. Bryselbout, P. Henner, J. Carsignol, E. Lichtfouse

引用次数: 52

Electrospray Ionisation Mass Spectrometry (ESI-MS): a powerful tool for the evaluation of chiral recognition in host-guest complexation 电喷雾电离质谱(ESI-MS):评估主客体络合中手性识别的有力工具

Analusis Pub Date : 2000-06-01 DOI: 10.1051/ANALUSIS:2000280259

Hélène Nierengarten, E. Leize, Christophe Garcia, G. Jeminet, A. Dorsselaer