Analusis最新文献_第7页

■ Quantitative gas detection with semiconductor micro-sensors and chemometrics ■采用半导体微传感器和化学计量学的定量气体检测

Analusis Pub Date : 2000-09-01 DOI: 10.1051/ANALUSIS:2000140

P. Breuil, N. Perdreau, C. Pijolat

引用次数: 6

3D quantitative structure activity relationships with CoRSA. Comparative receptor surface analysis. Application to calcium channel agonists 与CoRSA的三维定量结构活性关系。比较受体表面分析。应用于钙通道激动剂

Analusis Pub Date : 2000-09-01 DOI: 10.1051/ANALUSIS:2000141

O. Ivanciuc, T. Ivanciuc, D. Cabrol-Bass

{"title":"3D quantitative structure activity relationships with CoRSA. Comparative receptor surface analysis. Application to calcium channel agonists","authors":"O. Ivanciuc, T. Ivanciuc, D. Cabrol-Bass","doi":"10.1051/ANALUSIS:2000141","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000141","url":null,"abstract":"The in vitro or in vivo interaction between chemical compounds and their biological targets (transporters, receptors, ion channels, enzymes) can be efficiently predicted with the 3D QSAR models introduced in recent years. In this paper we describe CoRSA (comparative receptor surface analysis), a novel 3D QSAR algorithm that can be applied to compute structure-activity equations whenever the structure of the biological target is not known. Using the common steric and electrostatic features of the most active members of a series of compounds, CoRSA generates a virtual receptor model, represented as points on a surface complementary to the van der Waals surface of the set of compounds. The structural descriptors of the model are represented by the total interaction energies between each surface point of the virtual receptor and all atoms in a molecule. These descriptors are used in a partial least squares (PLS) data analysis to generate a structure-activity model. A highly significant CoRSA model was obtained for a set of compounds that act as calcium channel agonists for the guinea pig left atrium assay.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"18 1","pages":"637-642"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82178252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Influence of pH on the photodegradation kinetics under UV light of climbazole solutions pH对克里巴唑溶液紫外光降解动力学的影响

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000171

C. Couteau, M. Jadaud, F. Peigne, L. Coiffard

引用次数: 12

EPR spin trapping of superoxide from nitric oxide synthase 一氧化氮合酶超氧化物的EPR自旋捕获

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000280487

J. Vasquez-Vivar, J. Joseph, H. Karoui, Hao Zhang, J. Miller, P. Martásek

引用次数: 8

■ Real-time measurement of free radical production using specific electrochemical sensors: new insight into the consequences of O 2 - and NO flux ■使用特定电化学传感器实时测量自由基产生:对o2和NO通量后果的新见解

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000280493

P. Manning, M. Cookson, C. Eggett, C. Tolias, S. Read, A. Hunter, M. Tsatmali, A. Thody, E. Hillhouse, P. Shaw, C. McNeil

引用次数: 10

Influence of secondary interactions on high performance size exclusion chromatography. Application to the fractionation of landfill leachates 二次相互作用对高效粒径排阻色谱的影响。垃圾渗滤液分馏的应用

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000169

F. L. Coupannec, D. Morin, O. Sire, J. Peron

引用次数: 2

■ Design of electrochemical microsensors to monitor nitric oxide production in biological systems: a global compilation ■设计电化学微传感器以监测生物系统中一氧化氮的产生:全球汇编

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000280465

M. Pontie, F. Bedioui

引用次数: 8

■ Advantages and limits of the electrochemical method using Nafion and Ni-porphyrin-coated microelectrode to monitor NO release from cultured vascular cells ■利用Nafion和ni -卟啉包被微电极监测培养血管细胞NO释放的电化学方法的优点和局限性

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000280469

A. Brunet, C. Privat, O. Stepien, M. David‐Dufilho, J. Devynck, M. Devynck

引用次数: 9

■ Mathematical treatment of chemiluminescence data allowing an optimised kinetic analysis of vascular NAD(P)H-dependent superoxide anion production ■化学发光数据的数学处理，可以优化血管NAD(P) h依赖性超氧阴离子产生的动力学分析

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000280479

J. Iliou, N. Villeneuve, M. Fournet-Bourguignon, F. Robin, C. Jacquemin, V. Lestriez, C. Petit, A. Pillon, J. Vilaine

{"title":"■ Mathematical treatment of chemiluminescence data allowing an optimised kinetic analysis of vascular NAD(P)H-dependent superoxide anion production","authors":"J. Iliou, N. Villeneuve, M. Fournet-Bourguignon, F. Robin, C. Jacquemin, V. Lestriez, C. Petit, A. Pillon, J. Vilaine","doi":"10.1051/ANALUSIS:2000280479","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280479","url":null,"abstract":"Regulation of vascular redox homeostasis plays a central role in the control of vascular tone (e.g.: redox modulation of endothelial NO synthase and soluble guanylate cyclase activities). Superoxide anion is one of the main reactive oxygen species involved in the modulation of the redox status of the vessel. It is crucial to know if pathological situations or drug treatments are able to modify NAD(P)H oxidase-dependent production of O 2 - . As a steady-state approach is the only way for a valid assessment of these parameters, a mathematical treatment allowing visualisation of the linear portion of the initial velocity of O 2 - production was developed. Using two vascular preparations (rat aortic rings and mouse thoracic aortae), the chemiluminescence (CL) in the presence of lucigenin was recorded every second in the 15-30 minute period after successive injections of NADPH and superoxide dismutase (SOD). Because, both O 2 - and photons are labile and evanescent products, visualisation of the steady-state requires calculation of their cumulative production by integral calculus using a first order integral equation. The cumulative production of CL and/or O 2 - was plotted as a function of the NADPH concentrations, were determined from the linear portion of these plots. For a precise molar quantification of O 2 - production, a calibration curve of initial velocities using xanthine oxidase + xanthine as a source of O 2 - was plotted by comparing CL and cytochrome C reduction. The NADPH-dependent CL production exihibited Michaelian behavior and SOD acted as a noncompetitive inhibitor. For example, the values for NADPH oxidase in rat aorta were: Km = 237 ± 2μM; Vmax = 500-600 pmol O 2 - /min/ring.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"6 1","pages":"479-486"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88837343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Use of a chloride ion selective electrode as a tool for monitoring relatively fast kinetic processes. Application to the hydrolysis of yperite analogues in presence of oximate nucleophiles 使用氯离子选择电极作为监测相对快速动力学过程的工具。在邻氧亲核试剂存在下亚闪石类似物水解的应用

Analusis Pub Date : 2000-07-01 DOI: 10.1051/ANALUSIS:2000168

S. P. Rodrigues, L. Coppée, J. Toullec, G. Moutiers

引用次数: 1