Analusis最新文献_第10页

Determination of selenium in rat brain by synchrotron radiation X-ray fluorescence 同步辐射x射线荧光法测定大鼠脑中硒含量

Analusis Pub Date : 2000-06-01 DOI: 10.1051/ANALUSIS:2000121

N. Schulmann-Choron, P. Chevallier, J. Ceaurriz, C. Souleau

引用次数: 5

Poly(ethyleneglycol) in electrospray ionization (ESI) mass spectrometry 聚乙二醇在电喷雾电离(ESI)质谱中的应用

Analusis Pub Date : 2000-06-01 DOI: 10.1051/ANALUSIS:2000280263

S. Varray, J. Aubagnac, F. Lamaty, R. Lazaro, J. Martinez, C. Enjalbal

引用次数: 21

Determination of residual sodium content in silver and silver oxide composite materials 银和氧化银复合材料中残余钠含量的测定

Analusis Pub Date : 2000-06-01 DOI: 10.1051/ANALUSIS:2000119

R. Vivier, L. Muhr, H. Muhr, E. Plasari

引用次数: 1

Improved separation of rabbit liver metallothioneins by FPLC-ICP-MS: a comparison with the conventional anion-exchange chromatography 用hplc - icp - ms改进兔肝金属硫蛋白的分离:与传统阴离子交换色谱法的比较

Analusis Pub Date : 2000-06-01 DOI: 10.1051/ANALUSIS:2000280351

C. Ferrarello, M. Campa, H. G. Infante, M. L. F. Sánchez, A. Sanz-Medel

引用次数: 10

Evaluating the role of dibutyl-cAMP and Ca++ as an MT inducer in a clonal rat hepatoma cell 二丁基- camp和ca++在克隆大鼠肝癌细胞中作为MT诱导剂的作用

Analusis Pub Date : 2000-06-01 DOI: 10.1051/ANALUSIS:2000280391

M. Yang, K. Wan, Deng-yun Chen

{"title":"Evaluating the role of dibutyl-cAMP and Ca++ as an MT inducer in a clonal rat hepatoma cell","authors":"M. Yang, K. Wan, Deng-yun Chen","doi":"10.1051/ANALUSIS:2000280391","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280391","url":null,"abstract":"Metallothioneins (MT) is a low molecular weight, cysteine-rich, metal-binding protein that can be induced when cells and tissues are exposed to metal such as Cd, Zn, Cu and Hg. MT could also be induced by non-metallic stimuli including physiological, chemical and pathological stresses. It has been suggested that signalling molecules such as cAMP or Ca ++ might also induce MT. In the present study, the mode of induction of MT in the RH-35 cells was investigated with reference to the integrity of the cells. The degree of MT induction was measured by the Cd-saturation assay in which total Cd bound to the heat-stable MT was determined by the graphite furnace atomic absorption spectrometry. It was demonstrated that Zn and Cd could markedly increase cellular MT content. Alpha-amanitin, which blocks DNA transcription, can significantly decrease the Cd induced MT induction. MT induction was also lowered by cycloheximide, which blocks protein translation. However, dibutyl-cAMP, Ca ++ and Ca ++ in the presence of ionomycin were unable to induce cellular MT. Using this model, the results supported that notion that MT induction might not be mediated by cAMP or Ca ++ in this cell line.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"55 1","pages":"391-395"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88855602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Metallothioneins in the clam Ruditapes decussatus: an overview 蛤蚌中金属硫蛋白的研究进展

Analusis Pub Date : 2000-06-01 DOI: 10.1051/ANALUSIS:2000280386

M. Bebianno, M. Serafim, D. Simes

{"title":"Metallothioneins in the clam Ruditapes decussatus: an overview","authors":"M. Bebianno, M. Serafim, D. Simes","doi":"10.1051/ANALUSIS:2000280386","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280386","url":null,"abstract":"coastal waters. Due to its economic importance it is extensively cultured in many countries, and particularly in Portugal where its production represents > 80 % of total shellfish production. The selection of the clam R. decussatus as a bioindicator was a consequence of its economic importance and the need to use a filter-feeding bivalve to evaluate environmental changes of metal concentrations [1]. Like many other bivalves, this species resides in sediments and accumulates metal concentrations reflecting gradients of metal contamination in the surrounding environment [2,3,4]. This suggests that its tissues have mechanisms, related to its filter-feeding habit, that inhibit the toxic effects of these contaminants. Like many other molluscs R. decussatus has evolved a number of subcellular systems for accumulation, regulation and immobilization of metals. These include, among others, the binding of essential and pollutant metals to soluble ligands such as MT. These two-domain molecules are low molecular, heat-stable proteins of non-enzymatic nature that occur mainly in the cytoplasm. They have strong affinity to class B metal cations which enable them to be differentiated from most of the other proteins [5]. The induction of these proteins has, therefore, been proposed as a specific indicator and possible “early warning marker” for the detection of detrimental effects caused by exposure to excess of essential and pollutant metals. MT induction has been detected in the whole soft tissues, gills, digestive gland and remaining tissues of the clam R. decussatus after exposure to essential and toxic metals [4,6] and this paper represents an overview of MT function in this bivalve species. MT induction in laboratory experiments","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"32 1","pages":"386-390"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91208870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Evolution of camphor and others components in the essential oils of two labiate species during the biological cycle. 两种唇形动物精油中樟脑及其它成分在生物循环过程中的演化。

Analusis Pub Date : 2000-06-01 DOI: 10.1051/ANALUSIS:2000120

J. Kaloustian, A. Pauli, J. Pastor

引用次数: 19

Suitability of different plastic materials for head or nose spaces short term storage 适合不同塑料材料的头部或鼻子空间的短期存储

Analusis Pub Date : 2000-06-01 DOI: 10.1051/ANALUSIS:2000123

J. Pet'ka, P. Etiévant, G. Callement

引用次数: 32

■ Complexing properties of the β metallothionein domain with cadmium and/or zinc, studied by differential pulse polarography ■用差分脉冲极谱法研究了β金属硫蛋白与镉和/或锌的络合性质

Analusis Pub Date : 2000-06-01 DOI: 10.1051/ANALUSIS:2000280370

M. Dabrio, A. Rodríguez

{"title":"■ Complexing properties of the β metallothionein domain with cadmium and/or zinc, studied by differential pulse polarography","authors":"M. Dabrio, A. Rodríguez","doi":"10.1051/ANALUSIS:2000280370","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280370","url":null,"abstract":"An electrochemical study of the complexing properties of the β metallothionein domain with cadmium and/or zinc was performed at pH 8, using differential pulse polarography. The applied technique allows one to obtain well-defined peaks corresponding to the free metal ions as well as providing evidence that different forms of complexation of Cd(II) and Zn(II) with the β domain exist. The influence of sequential additions of Cd and/or Zn to the β domain was investigated. The β domain is capable of complexing cadmium and/or zinc, separately or after simultaneous additions, resulting in complexes with a of M:β ratio of 3:1, but which possess different characteristics. In the case of binary mixtures, Cd + β or Zn + β, pure complexes with a probable stoichiometry of Cd3β and Zn3β are formed, whereas in the case of tertiary mixtures, β + [Cd + Zn] mixed cadmium and zinc complexes Cd2Znβ and CdZn2β prevail. When one of the two metal ions is added to a solution containing the β domain complex with the other cation, it is incorporated into the molecule resulting in a reorganisation, which is illustrated by changes in the polarographic responses. Polarographic results have been compared with those obtained by electrospray ionisation mass spectrometry, providing the molecular mass of compounds under similar experimental conditions. Different polarographic peaks are found for either pure or mixed complexes.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"84 1","pages":"370-381"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90363843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Effect of heat treatment on metallothionein isoforms using capillary zone electrophoresis 毛细管区带电泳研究热处理对金属硫蛋白异构体的影响

Analusis Pub Date : 2000-06-01 DOI: 10.1051/ANALUSIS:2000280366

K. Kubo, Y. Sakita, T. Minami

{"title":"Effect of heat treatment on metallothionein isoforms using capillary zone electrophoresis","authors":"K. Kubo, Y. Sakita, T. Minami","doi":"10.1051/ANALUSIS:2000280366","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280366","url":null,"abstract":"The effect of heat treatment on metallothionein (MT) isoforms was observed by using capillary zone electrophoresis (CZE) at a neutral pH. Commercially available standard MT-1 preparation isolated from rabbit liver present as Cd and Zn mixed form was used as MT standard for the experiments in CZE. The MT-1 preparation was detected as two major peaks MT-1 and MT-2 identified from the migration times corresponded with those of purified isoforms and several minor peaks by CZE using a polyacrylamide-coated capillary at pH 7.4. The peak height of MT-1 of the standard MT-1 specimen decreased gradually from those of the non-heated specimen by heat treatment at 100°C until 4 min, but the peak of MT-2 did not decrease. In the cytosol fraction obtained from Zn-injected mouse liver, many peaks including MT-1 and MT-2 were detected in the specimen without heat treatment. The specimen heated at 100°C for 15 s had a stable baseline and the peaks of both MT-1 and MT-2 in cytosol fraction decreased gradually by heat treatment on time dependent manner. However, the ratio of peak heights of MT-1 and MT-2 did not change. From these results, the ratio of MT-1 and MT-2 in cytosol fraction is able to be observed well on CZE analysis after heat treatment.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"21 1","pages":"366-369"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86241528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10