C. Ferrarello, M. Campa, H. G. Infante, M. L. F. Sánchez, A. Sanz-Medel
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引用次数: 10
摘要
研究了快速蛋白液相色谱法(Mono Q HR 5/5色谱柱)和传统阴离子交换色谱法(Protein Pak DEAE 5PW色谱柱)分离兔肝脏金属硫蛋白和Cd形态的分析性能，并对结果进行了比较。与传统的阴离子交换柱Protein Pak DEAE 5PW相比，Mono Q柱在更短的分离时间内具有更高的分辨率和重现性。因此，FPLC被认为是研究金属硫蛋白多态性的一个非常有用的工具。
Improved separation of rabbit liver metallothioneins by FPLC-ICP-MS: a comparison with the conventional anion-exchange chromatography
The analytical performance of the Fast - Protein Liquid Chromatography (FPLC) (on a Mono Q HR 5/5 column) and the conventional AnionExchange Chromatography (on a Protein Pak DEAE 5PW column) to realise rabbit liver metallothioneins separations and Cd speciation has been investigated and the results are compared. The Mono Q column was found to provide higher resolution and reproducibility in shorter separation times than Protein Pak DEAE 5PW, a conventional anion-exchange column. Thus, FPLC is proposed as a very useful tool for the study of the polymorphism of metallothioneins.
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