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Effect of sample collection site on semen parameters of healthy young volunteers. 采集地点对健康青年志愿者精液参数的影响。
Archives of andrology Pub Date : 2007-03-01 DOI: 10.1080/01485010600908454
S Oshio, M Yotsukura, Y Tohyama, M Iwabuchi, Y Adachi, Y Ashizawa, H Matsuda, T Iiyama, H Shimizu, H Tomomasa, S Yoshida, K Takeda, T Umeda
{"title":"Effect of sample collection site on semen parameters of healthy young volunteers.","authors":"S Oshio,&nbsp;M Yotsukura,&nbsp;Y Tohyama,&nbsp;M Iwabuchi,&nbsp;Y Adachi,&nbsp;Y Ashizawa,&nbsp;H Matsuda,&nbsp;T Iiyama,&nbsp;H Shimizu,&nbsp;H Tomomasa,&nbsp;S Yoshida,&nbsp;K Takeda,&nbsp;T Umeda","doi":"10.1080/01485010600908454","DOIUrl":"https://doi.org/10.1080/01485010600908454","url":null,"abstract":"<p><p>The effect of sample collection site on semen parameters in ten men aged between 22 and 24 years was investigated. Sperm was collected at two sites: in a university hospital restroom for general use and in a one-person hospital room. Samples were collected from the same individual twice, with an interval of two weeks between collections. Semen parameters for the two sites were compared. Samples were collected after a minimum of three days and not longer than seven days of sexual abstinence. Sperm concentration did not differ significantly between the university hospital restroom location (86.8 +/- 25.4 x 10(6)/ml; mean +/- standard deviation) and the private hospital room (97.1 +/- 72.0 x 10(6)/ml). There was no difference in the total motile sperm count or daily sperm production between the collection sites. These results suggest that the collection site has little effect on semen parameters.</p>","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"53 2","pages":"53-8"},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010600908454","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26682849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Sole use of sucrose in human sperm cryopreservation. 在人类精子冷冻保存中唯一使用蔗糖。
Archives of andrology Pub Date : 2007-03-01 DOI: 10.1080/01485010701225675
A M Hossain, C O Osuamkpe
{"title":"Sole use of sucrose in human sperm cryopreservation.","authors":"A M Hossain,&nbsp;C O Osuamkpe","doi":"10.1080/01485010701225675","DOIUrl":"https://doi.org/10.1080/01485010701225675","url":null,"abstract":"<p><p>Glycerol alone or in combination with other additives is one of the most widely used and successful cryoprotectants for human sperm. The glycerol method requires rigorous post thaw sample washing for use in ART and this may lead to low sperm yield from oligospermic samples. In this study the feasibility of the use of sucrose in sperm cryopreservation was explored. Sucrose as cryoprotectant was combined with direct plunging of sample into liquid nitrogen (vitrification) as a freezing method. Sucrose treated sperm from normozoospermic and severly oligozoospermic samples underwent rapid freeze and thaw. Motility and viability were evaluated before freezing (after sucrose equilibration) as well as post freezing (after thaw). The 100 mM concentration of sucrose showed better cryoprotectant features compared to that of higher concentrations (200-1000 mM). Sucrose (100 mM)treated sperm maintained low but acceptable motility (30%) and satisfactory viability (60%) after freezing and thawing. The cryoprotectant capacity of sucrose for normozoospermic and oligozoospermic samples were identical. The sucrose method utilizes rapid freezing of a micro volume of sample and thus quickly freezes, thaws, and maximizes recovery of the sperm from the sample.</p>","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"53 2","pages":"99-103"},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010701225675","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26682778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Development and retest reliability of a German version of the Sexual Behaviour Questionnaire (SBQ-G). 德国版性行为问卷(SBQ-G)的研制与重测信度。
Archives of andrology Pub Date : 2007-03-01 DOI: 10.1080/01485010600915186
M J Müller
{"title":"Development and retest reliability of a German version of the Sexual Behaviour Questionnaire (SBQ-G).","authors":"M J Müller","doi":"10.1080/01485010600915186","DOIUrl":"https://doi.org/10.1080/01485010600915186","url":null,"abstract":"<p><p>A German version of the Sexual Behaviour Questionnaire (SBQ-G) is a short self-rating instrument covering the four domains of sexual function using colloquial words. Frequency of sexual dysfunction was counted and chance-corrected retest reliability was calculated. Eleven women and 12 men participated. Reception of the SBQ-G was favourable and more females than males reported at least moderate sexual dysfunction. Sexual arousal and ability to enjoy sex showed the lowest retest stability. The SBQ-G is a recommendable clinical instrument with high practicability and satisfactory retest reliability. However, gender differences and a high variability of sexual functioning in healthy subjects should be kept in mind.</p>","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"53 2","pages":"67-9"},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010600915186","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26682854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Regulation of steroidogenesis by Cordyceps sinensis mycelium extracted fractions with (hCG) treatment in mouse Leydig cells. 冬虫夏草菌丝体提取物(hCG)对小鼠间质细胞类固醇生成的调节作用。
Archives of andrology Pub Date : 2007-03-01 DOI: 10.1080/01485010600915236
K L Wong, E Cheung So, C C Chen, R S C Wu, B-M Huang
{"title":"Regulation of steroidogenesis by Cordyceps sinensis mycelium extracted fractions with (hCG) treatment in mouse Leydig cells.","authors":"K L Wong,&nbsp;E Cheung So,&nbsp;C C Chen,&nbsp;R S C Wu,&nbsp;B-M Huang","doi":"10.1080/01485010600915236","DOIUrl":"https://doi.org/10.1080/01485010600915236","url":null,"abstract":"<p><p>The in vitro effect of extracted fractions of Cordyceps sinensis (CS) mycelium on hCG-treated testosterone production from purified normal mouse Leydig cells was examined. Different fractions extracted from CS (F1-water soluble polysaccharide, F2- water soluble protein and F3- poorly water soluble polysaccharide, and protein) were added to Leydig cells with hCG, and the production of testosterone was determined by radioimmunoassay (RIA). Testosterone productions stimulated by hCG in mouse Leydig cells were suppressed by F2 at 10 mg/ml and F3 at doses from 3 to 10 mg/ml, respectively. F2 and F3 at 10 mg/ml did inhibit dbcAMP-stimulated testosterone productions which indicated that F2 and F3 might affect steroidogenesis at the site after the formation of cyclic AMP. Finally, cycloheximide inhibited F2- and F3-treated mouse Leydig cell testosterone production.</p>","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"53 2","pages":"75-7"},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010600915236","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26682855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Finasteride to evaluate the efficacy of dutasteride in the management of patients with lower urinary tract symptoms and enlarged prostate. 非那雄胺评价度他雄胺治疗下尿路症状和前列腺肥大患者的疗效。
Archives of andrology Pub Date : 2007-01-01 DOI: 10.1080/01485010600888953
I R Ravish, R B Nerli, S S Amarkhed
{"title":"Finasteride to evaluate the efficacy of dutasteride in the management of patients with lower urinary tract symptoms and enlarged prostate.","authors":"I R Ravish,&nbsp;R B Nerli,&nbsp;S S Amarkhed","doi":"10.1080/01485010600888953","DOIUrl":"https://doi.org/10.1080/01485010600888953","url":null,"abstract":"<p><p>Patients with lower urinary tract symptoms and enlarged prostate were randomized to receive, in a double blind fashion, either Finasteride 5 mg/day or Dutasteride 0.5 mg/day for a period of 12 weeks. IPSS score, Qmax, and Quality of Life were assessed at the end of 0 and 12 week periods. Dutasteride significantly improved Qmax, reduced IPSS score, and improved Quality of Life as compared to Finasteride at the end of the 12-week period. Dutasteride with its inhibitory effects on type 1 and 2 5alpha-reductase, produces significantly better results than Finasteride.</p>","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"53 1","pages":"17-20"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010600888953","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26207906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
A successful pregnancy outcome using testicular sperm from an infertile male pretreated with HCG. 使用经HCG预处理的不育男性睾丸精子成功怀孕的结果。
Archives of andrology Pub Date : 2007-01-01 DOI: 10.1080/01485010600889076
Y-X Cao, Z-G Zhang
{"title":"A successful pregnancy outcome using testicular sperm from an infertile male pretreated with HCG.","authors":"Y-X Cao,&nbsp;Z-G Zhang","doi":"10.1080/01485010600889076","DOIUrl":"https://doi.org/10.1080/01485010600889076","url":null,"abstract":"<p><p>A very rare case of successful pregnancy was reported using testicular sperm from a severely infertile man given the treatment of hCG in advance. Prior to treatment, no sperm were present in the ejaculate and testicle. hCG was administrated to the patient for spermatogenesis. After a period of hCG treatment, a few immature sperm were observed in the minced testicular specimen. A successful pregnancy was established by ICSI using the sperm. Treatment by administration of hCG to generate sperm followed by ICSI for the azoospermia patients was effective and practicable.</p>","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"53 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010600889076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26207942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Relationships between levels of estradiol and testosterone in seminal plasma and GSTM1 polymorphism in infertile men. 不育男性精浆雌二醇和睾酮水平与GSTM1多态性的关系
Archives of andrology Pub Date : 2007-01-01 DOI: 10.1080/01485010600889134
I Onaran, B Aydemir, A R Kiziler, T Demiryurek, B Alici
{"title":"Relationships between levels of estradiol and testosterone in seminal plasma and GSTM1 polymorphism in infertile men.","authors":"I Onaran,&nbsp;B Aydemir,&nbsp;A R Kiziler,&nbsp;T Demiryurek,&nbsp;B Alici","doi":"10.1080/01485010600889134","DOIUrl":"https://doi.org/10.1080/01485010600889134","url":null,"abstract":"<p><p>Glutathione S-transferase M1 (GSTM1) enzyme serves as a steroid-binding protein by its ability to bind to testosterone and estradiol. The levels of total estradiol and testosterone were measured by using an electrochemiluminescence immunoassay in serum and seminal plasma from 103 subjects including 62 subfertile patients. GSTM1 polymorphism was examined using polymerase chain reaction. The estradiol and testosterone levels in seminal plasma were not different in control and subfertile subjects. No role for GSTM1 enzyme as a steroid-binding protein seemed likely as there was also no significant difference in seminal plasma estradiol and testosterone levels according to GSTM1 genotype. Significant positive correlations were found between seminal estradiol and serum estradiol in infertile males, and between seminal testosterone and serum testosterone in fertile males, independent of GSTM1 genotype. GSTM1 polymorphism is not a genetic risk factor of seminal estradiol and testosterone levels in infertile males although further studies are warranted.</p>","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"53 1","pages":"13-6"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010600889134","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26207945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Severe hypospadias with micropenis, case of confused sex, psychosocial changes and treatment. 重度尿道下裂伴小阴茎，性别混淆病例，心理社会变化及治疗。
Archives of andrology Pub Date : 2007-01-01 DOI: 10.1080/01485010600889068
I R Ravish, R B Nerli, S M Kamat
{"title":"Severe hypospadias with micropenis, case of confused sex, psychosocial changes and treatment.","authors":"I R Ravish,&nbsp;R B Nerli,&nbsp;S M Kamat","doi":"10.1080/01485010600889068","DOIUrl":"https://doi.org/10.1080/01485010600889068","url":null,"abstract":"<p><p>Disorders such as severe hypospadias presenting as ambiguous genitalia have serious and potentially life-long consequences for affected individuals and, depending on the underlying cause, are likely to entail surgery in childhood and in later life, psychosocial and psychosexual support, and possible fertility treatment including assisted conception. Genetic males with severe hypospadias who were wrongly brought up as females formed the study group. They were reassigned as males and underwent surgery for correction of hypospadias. Three children with severe hypospadias were reassigned to male gender. Psychosocial changes were made and psychosexual counseling was done. All three have adjusted to their new environment and gender. It is preferable to reassign these children who are genetic males but wrongly assigned as females at birth. Repair of hypospadias, psychosocial changes, and psychosexual counseling will help these children to reorient themselves.</p>","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"53 1","pages":"9-12"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010600889068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26207943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Testicular sperm retrieval method: histological effects of location of incision. 睾丸取精方法:切口位置的组织学影响。
Archives of andrology Pub Date : 2007-01-01 DOI: 10.1080/01485010600888938
B H Gümüs, Y Z Atesçi, O Ucer, T C Karatas, C Buyuksu, N Nese, A R Kandiloglu
{"title":"Testicular sperm retrieval method: histological effects of location of incision.","authors":"B H Gümüs,&nbsp;Y Z Atesçi,&nbsp;O Ucer,&nbsp;T C Karatas,&nbsp;C Buyuksu,&nbsp;N Nese,&nbsp;A R Kandiloglu","doi":"10.1080/01485010600888938","DOIUrl":"https://doi.org/10.1080/01485010600888938","url":null,"abstract":"<p><p>This research was conducted to evaluate the effects of a transverse vs. longitudinal incision for testicular sperm retrieval. Rats were divided into 4 groups: I: control, II: sham operation, III: longitudinal incision TSRM, IV: transverse incision TSRM. Group II (sham operation) had a dissection of left testis/spermatic cord, then closure of wound. Group III underwent dissection of left testis, then a \"longitudinal\" incision (15 mm long) of testis, which was fully opened then closed again and sutured with 5-0 Vicryl sutures. Group IV underwent dissection of the left testis, opening of the testis with a \"transverse\" incision, then closure with 5-0 Vicryl. The seminiferous tubule diameter was 0.118-0.224 mm in all groups. Inflammation and abscess formation occurred in one testis each in the sham and longitudinal incision groups, and in two testes in the transverse incision group. There were no differences in histopathology or scoring between the longitudinal and the transverse incision.</p>","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"53 1","pages":"33-5"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010600888938","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26207912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of sperm chromatin quality and screening of Y chromosome microdeletions in Greek males with severe oligozoospermia. 希腊严重少精症男性精子染色质质量评价及Y染色体微缺失筛查。
Archives of andrology Pub Date : 2007-01-01 DOI: 10.1080/01485010600889159
D Mantas, R Angelopoulou, P Msaouel, K Plastira
{"title":"Evaluation of sperm chromatin quality and screening of Y chromosome microdeletions in Greek males with severe oligozoospermia.","authors":"D Mantas,&nbsp;R Angelopoulou,&nbsp;P Msaouel,&nbsp;K Plastira","doi":"10.1080/01485010600889159","DOIUrl":"https://doi.org/10.1080/01485010600889159","url":null,"abstract":"<p><p>Normal (n = 20) and abnormal (n = 21) semen samples were explored for possible relationships between conventional semen parameters, chromatin status, and microdeletions in the Y chromosome. DNA fragmentation was detected by the terminal deoxynucleotidyl trasferase-mediated dUTP-nick end labelling (TUNEL) assay, chromatin condensation, and DNA packaging quality were assessed by chromomycin A3 (CMA(3)) staining. All men were investigated for Y chromosome microdeletions using polymerase chain reaction (PCR). No deletions were detected in 21 severely oligozoospermic men for the three screened regions (AZFa, AZFb, and AZFc). Men with normal semen parameters showed better chromatin condensation. Spermatozoa with low motility were more likely to contain loosely packaged chromatin. In the abnormal semen group, DNA fragmentation (TUNEL) correlated significantly with sperm motility, concentration, and chromatin packaging assessed by chromomycin A3. However sperm morphology did not correlate significantly with TUNEL and CMA(3) staining.</p>","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"53 1","pages":"5-8"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010600889159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26207944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
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