Archives of andrology最新文献_第10页

Somatic angiotensin converting enzyme in varicocele. 精索静脉曲张的体血管紧张素转换酶。

Archives of andrology Pub Date : 2006-07-01 DOI: 10.1080/01485010500503629

R Asci, S Sarikaya, R Buyukalpelli, A Yilmaz, A Bedir

{"title":"Somatic angiotensin converting enzyme in varicocele.","authors":"R Asci, S Sarikaya, R Buyukalpelli, A Yilmaz, A Bedir","doi":"10.1080/01485010500503629","DOIUrl":"https://doi.org/10.1080/01485010500503629","url":null,"abstract":"The ACE is found as two isozymes in the body. A somatic isozyme found in blood and several other tissues, and a testis-specific isozyme found only in developing spermatids and mature sperm. In this study, we investigated the ACE activity in left spermatic vein blood samples of infertile patients with varicocele and its correlation to spermatologic parameters. The somatic ACE activities were determined in the peripheral and left spermatic vein blood samples from 31 infertile patients who underwent variococelectomy, and 11 fertile control subjects underwent left inguinal herniorraphy. The somatic ACE activity was measured by kinetic spectrophotometric assay. Semen analyses were performed according to WHO guidelines. The mean somatic ACE activities of peripheral and left spermatic veins of the varicocele group were 60.3 +/- 23.0 and 60.2 +/- 23.2 U/L, respectively. In control group, peripheral and left spermatic vein ACE activities were found as 56.8 +/- 17.1 and 56.5 +/- 15.5 U/L, respectively. There was no significant difference between the ACE activity in peripheral and left spermatic vein blood sample from the varicocele and control group. There was no statistically significant correlation between the spermatologic parameters and ACE activities in the spermatic and peripheral vein in both of varicocele and control groups. As a result, it may be suggested that the somatic ACE has no causative role in pathophysiology of varicocele and varicocele related infertility.","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"52 4","pages":"329-34"},"PeriodicalIF":0.0,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010500503629","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26048800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular andrology as related to sperm DNA fragmentation/sperm chromatin biotechnology. 与精子DNA断裂/精子染色质生物技术相关的分子男科学。

Archives of andrology Pub Date : 2006-07-01 DOI: 10.1080/01485010600668363

A Shafik, A A Shafik, I Shafik, O El Sibai

{"title":"Molecular andrology as related to sperm DNA fragmentation/sperm chromatin biotechnology.","authors":"A Shafik, A A Shafik, I Shafik, O El Sibai","doi":"10.1080/01485010600668363","DOIUrl":"https://doi.org/10.1080/01485010600668363","url":null,"abstract":"Genetic male infertility occurs throughout the life cycle from genetic traits carried by the sperm, to fertilization and post-fertilization genome alterations, and subsequent developmental changes in the blastocyst and fetus as well as errors in meiosis and abnormalities in spermatogenesis/spermatogenesis. Genes encoding proteins for normal development include SRY, SOX9, INSL3 and LGR8. Genetic abnormalities affect spermatogenesis whereas polymorphisms affect receptor affinity and hormone bioactivity. Transgenic animal models, the human genome project, and other techniques have identified numerous genes related to male fertility. Several techniques have been developed to measure the amount of sperm DNA damage in an effort to identify more objective parameters for evaluation of infertile men. The integrity of sperm DNA influences a couple's fertility and helps predict the chances of pregnancy and its successful outcome. The available tests of sperm DNA damage require additional large-scale clinical trials before their integration into routine clinical practice. The physiological/molecular integrity of sperm DNA is a novel parameter of semen quality and a potential fertility predictor. Although DNA integrity assessment appears to be a logical biomarker of sperm quality, it is not being assessed as a routine part of semen analysis by clinical andrologists. Extensive investigation has been conducted for the comparative evaluation of these techniques. However, some of these techniques require expensive instrumentation for optimal and unbiased analysis, are labor intensive, or require the use of enzymes whose activity and accessibility to DNA breaks may be irregular. Thus, these techniques are recommended for basic research rather than for routine andrology laboratories.","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"52 4","pages":"299-310"},"PeriodicalIF":0.0,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010600668363","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26048947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

In vitro effects of epristeride on sperm in rats, dogs and man. 爱普司特胺对大鼠、狗和人精子的体外影响。

Archives of andrology Pub Date : 2006-05-01 DOI: 10.1080/01485010500379830

J H Wu, Z Y Sun, L Cao

引用次数: 2

Effect of lisnopril, an angiotensin converting enzyme (ACE) inhibitor on spermatogenesis in rats. 血管紧张素转换酶抑制剂来诺普利对大鼠精子发生的影响。

Archives of andrology Pub Date : 2006-05-01 DOI: 10.1080/01485010500398012

B N Okeahialam, K Amadi, A S Ameh

引用次数: 18

Evaluation of DAZ microdeletions in 34 infertile men. 34例不育男性DAZ微缺失的评价。

Archives of andrology Pub Date : 2006-05-01 DOI: 10.1080/01485010500316261

N Bouayed Abdelmoula, A Sallemi, N Chakroun, L Keskes, A Amouri, T Rebai

{"title":"Evaluation of DAZ microdeletions in 34 infertile men.","authors":"N Bouayed Abdelmoula, A Sallemi, N Chakroun, L Keskes, A Amouri, T Rebai","doi":"10.1080/01485010500316261","DOIUrl":"https://doi.org/10.1080/01485010500316261","url":null,"abstract":"Microdeletions in Yq11 are a common molecular cause of spermatogenic failure in men and are recurrently detected in about 10-15% of idiopathic azoospermia and severe oligozoospermia. Screening for AZF microdeletions is often performed by multiplex PCR. AZFc deletions, involving the DAZ gene, form the majority of these deletions. The aim of this study was to evaluate in a group of 34 Tunisian infertile patients (16 oligozoospermic and 18 azoospermic men) the prevalence of DAZ microdeletions using a rapid molecular strategy: the PCR-DGGE method based on the high degree of homology between the DAZ gene and its autosomally equivalent DAZLA gene. DAZ microdeletions were detected in 8.8% of patients. The three deleted patients have a 46, XY karyotype. Two of them were azoospermic and the other had an extreme oligo-asthenoteratozoospermia with a predominant abnormality: small round head spermatozoa (Y46). Our findings suggest that PCR-DGGE method, for detection of DAZ gene deletion, could be particularly useful as a first step in the diagnosis workup of nonobstructive azoospermia and severe oligozoospermia for three reasons. First, it is a simple and fast system; second, DAZ microdeletions are the most common Y deletions; and third, partial DAZ microdeletions and mosaicism may be recognized by PCR-DGGE while only deletions removing the whole DAZ gene cluster can be detected by STS-PCR [211]. Nevertheless, this procedure has limitations because other deletions of AZFa and AZFb may go undetected. Therefore, molecular investigation by multiplex PCR must be conducted in a second step according to European guidelines for the molecular diagnosis of Y chromosome microdeletions, particularly before ICSI procedures.","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"52 3","pages":"175-8"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010500316261","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25937372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Screening of Y chromosome microdeletions in Tunisian infertile men. 突尼斯不育男性Y染色体微缺失的筛查。

Archives of andrology Pub Date : 2006-05-01 DOI: 10.1080/01485010500397964

L Hadj-Kacem, H Hadj-Kacem, H Ayadi, L Ammar-Keskes, N Chakroun-Fki, T Rebai, A Bahloul, M N Mhiri

{"title":"Screening of Y chromosome microdeletions in Tunisian infertile men.","authors":"L Hadj-Kacem, H Hadj-Kacem, H Ayadi, L Ammar-Keskes, N Chakroun-Fki, T Rebai, A Bahloul, M N Mhiri","doi":"10.1080/01485010500397964","DOIUrl":"https://doi.org/10.1080/01485010500397964","url":null,"abstract":"The aim of this study was to establish the prevalence of Y chromosomal microdeletions in infertile Tunisian men. Three groups of infertile men, 65 normospermic, 53 oligozoospermic and 45 azoospermic, were tested for Yq microdeletions detection by multiplex polymerase chain reaction (PCR) using specific Y chromosome AZF regions tagged site markers (STS). One group of 13 healthy men was used as the control group. Six STS were tested (2 in each AZF region). The general prevalence of AZF microdeletions was 16%; in azoospermia and severe oligospermia groups, it was higher (29% and 30.5%, respectively). Significant differences were found with moderate oligospermic and normospermic groups (p < 0,05). AZFc microdeletions were the most frequent, and 55% of AZFc deleted patients were oligospermic. No deletions were detected in the control group. These results add to the growing literature data, showing that microdeletions of the Y chromosome is an important cause of severe spermatogenetic defect and confirm that deletion in AZFc region is the most common and is compatible with residual spermatogenesis.","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"52 3","pages":"169-74"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010500397964","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25937374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Chronic propranolol treatment causes desensitization of the steroidogenic response in testicular interstitial cells but does not alter protein kinase C. 慢性心得安治疗引起睾丸间质细胞的类固醇反应脱敏，但不改变蛋白激酶C。

Archives of andrology Pub Date : 2006-05-01 DOI: 10.1080/01485010500397931

M C C Martins, D P Udrisar, D M C Rego, J S B C Vieira, M I Wanderley

{"title":"Chronic propranolol treatment causes desensitization of the steroidogenic response in testicular interstitial cells but does not alter protein kinase C.","authors":"M C C Martins, D P Udrisar, D M C Rego, J S B C Vieira, M I Wanderley","doi":"10.1080/01485010500397931","DOIUrl":"https://doi.org/10.1080/01485010500397931","url":null,"abstract":"We investigated effects of chronic propranolol treatment on the secretory response of rat testicular interstitial cells (testosterone secretion) to subsequent in vitro stimulation with activators of protein kinase-C (PK-C) (L-propranolol, phorbol 12, 13-dibutyrate (PDBu), LHRH) or activators of protein kinase A (PK-A), (hCG or dibutyryl cAMP (dbcAMP)). We determined [3H]PDBu binding and PK-C activity in these cells. Treatment of rats with propranolol (Inderal 500 mg/L of water for 5 weeks) reduced by 48%, 50% and 29% the L-propranolol-, LHRH- or PDBu-induced testosterone secretion, respectively, when compared to cells from controls. This desensitization in testosterone secretion in vitro was also present when the testicular interstitial cells were stimulated with hCG or dbcAMP (secretion decreased by 65%/57%, respectively, when compared to cells from control rats). Challenging the cells originated from rats that received propranolol chronically with the addition in vitro of propranolol resulted in an additional reduction of the hCG/dbcAMP-stimulated testosterone secretion. Chronic propranolol-induced desensitization was not associated with a loss in [3H]PDBu binding or a decrease in PK-C activity. Chronic propranolol-induced desensitization can be uncoupled from down-regulation of protein kinase C. The effector responsible for the desensitization could be distal to the protein kinase C and protein kinase A.","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"52 3","pages":"215-21"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010500397931","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25939581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Viability and fecundity of human semen specimens cryostored and transported at 5 degrees C using the Bio-Tranz shipping. 用Bio-Tranz运输技术在5℃下冷冻和运输人类精液标本的生存力和生殖力。

Archives of andrology Pub Date : 2006-05-01 DOI: 10.1080/01485010500428413

P M Zavos, P N Zavos, K Kaskar, J R Correa-Perez, S Koundouros

{"title":"Viability and fecundity of human semen specimens cryostored and transported at 5 degrees C using the Bio-Tranz shipping.","authors":"P M Zavos, P N Zavos, K Kaskar, J R Correa-Perez, S Koundouros","doi":"10.1080/01485010500428413","DOIUrl":"https://doi.org/10.1080/01485010500428413","url":null,"abstract":"This study was designed to assess the viability and fecundity of semen stored at 5 degrees C for 24 hours using the Bio-Tranz shipping system. Semen specimens were assessed for motility and sperm membrane integrity at the time of collection and 24 hours after storage in the Bio-Tranz. In group 1 (n = 61), specimens were diluted in TYB, processed and used for intrauterine insemination (IUI), leaving an aliquot for storage for 24 hours in the Bio-Tranz. In group 2 (n = 67), specimens were diluted in TYB, stored for 24 hours in the Bio-Tranz and then processed and used for IUI. In both groups, the total motile sperm used for IUI was similar and the women that underwent IUI were standardized for ovulation prediction and time of insemination. The overall sperm characteristics between the two groups were within normal range. Significant decreases were noted in sperm motility and membrane integrity in both groups after storage. Similar pregnancy rates were obtained between the two patient populations. The use of the Bio-Tranz shipper is extremely convenient for patients requiring semen evaluation, cryostorage or IUI and other assisted reproductive technologies.","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"52 3","pages":"149-54"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010500428413","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25937371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

High inguinal loupe-assisted varicocelectomy for subfertile men with varicococeles: technical feasibility, clinical outcomes and complications. 高腹股沟镜辅助精索静脉曲张切除术治疗低生育能力男性精索静脉曲张:技术可行性、临床结果及并发症。

Archives of andrology Pub Date : 2006-05-01 DOI: 10.1080/01485010500428389

M-L Hsieh, S T Huang, Y Chen, H C Huang, T-H Wang, S-H Chu, P L Chang

{"title":"High inguinal loupe-assisted varicocelectomy for subfertile men with varicococeles: technical feasibility, clinical outcomes and complications.","authors":"M-L Hsieh, S T Huang, Y Chen, H C Huang, T-H Wang, S-H Chu, P L Chang","doi":"10.1080/01485010500428389","DOIUrl":"https://doi.org/10.1080/01485010500428389","url":null,"abstract":"254 consecutive patients underwent high inguinal loupe-assisted varicocelectomy. All patients had at least a one year history of infertility with abnormal semen parameters and physical examination and/or color Doppler ultrasound proven varicocele. To facilitate the procedure, an x 3.0 loupe was used during spermatic cord dissection near or at level of internal inguinal ring. Semen analysis and physical examination were performed at 3 monthly intervals. No intra-operative complications occurred. The most common post-operative complications were transient scrotal pain and stitch reaction, occurring in 12% and 4% of men, respectively. Only one permanent and two transient hydroceles were observed. Recurrent or persistent varicocele was identified by physical examination and color Doppler in 5 varicocelectomies (1.4%), and by color Doppler only in 6 varicocelectomies (1.7%). Sperm motility increased from 30 +/- 8% to 46 +/- 20%, and sperm concentration. (10(6)/cc) increased from 24 +/- 18 to 41 +/- 28. The one-year pregnancy rate was 37%. High inguinal loupe-assisted varicocelectomy is a safe, simple, and effective treatment for varicocele.","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"52 3","pages":"179-83"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010500428389","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25937373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Efficacy of sildenafil for Japanese patients with audio-visual sexual stimulation (AVSS) test by the RigiScan Plus. 西地那非对日本患者视听性刺激(AVSS)试验的疗效

Archives of andrology Pub Date : 2006-05-01 DOI: 10.1080/01485010500379889

J Soh, Y Naya, S Ushijima, Y Naitoh, A Ochiai, Y Mizutani, A Kawauchi, T Miki, T Fujiwara

引用次数: 3