Archives of andrology最新文献_第9页

Effects of endurance training on antioxidant defense mechanisms and lipid peroxidation in testis of rats. 耐力训练对大鼠睾丸抗氧化防御机制及脂质过氧化的影响。

Archives of andrology Pub Date : 2006-07-01 DOI: 10.1080/01485010500503587

Y Aksoy, T Yapanoğlu, H Aksoy, B Demircan, N Oztaşan, E Canakçi, I Malkoç

{"title":"Effects of endurance training on antioxidant defense mechanisms and lipid peroxidation in testis of rats.","authors":"Y Aksoy, T Yapanoğlu, H Aksoy, B Demircan, N Oztaşan, E Canakçi, I Malkoç","doi":"10.1080/01485010500503587","DOIUrl":"https://doi.org/10.1080/01485010500503587","url":null,"abstract":"Male rats were equally divided into trained rest (TR), trained exhaustive exercise (TE), untrained rest (UR), and untrained exhaustive exercise (UE). Endurance training consisted of treadmill running for 1.5 h/d, 5 days a week for 8 weeks reaching the speed of 2.1 km/h at the fortieth week. For acute exhaustive exercise, graded treadmill running was conducted reaching the speed of 2.1 km/h at 95th min, 10% uphill, continued until exhaustion. Testicular tissue malondialdehyde (MDA), antioxidant potential (AOP) levels, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), glutathione reductase (GR) and catalase (CAT) activities were determined. There was a slight decrease, but not significant, in the SOD activity in UE group compared to TE and TR groups. Activity of GSH-Px decreased in the UE group compared to UR, TR and TE groups. Acute exhaustive exercise did not affect testicular tissue GSH-Px activity in trained rats. Testicular tissue GST activity of the UE group was similar to TE group, but lower than UR and TR groups. In UE group, testicular tissue AOP values were lower than UR, TR and TE groups. The oxidative effects of acute exhaustive exercise on the rat testis decreased with endurance training. Endurance training prevents oxidative injuries by eliminating oxygen radicals and inhibiting lipid peroxidation via preventing decreases in antioxidant enzyme activities.","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"52 4","pages":"319-23"},"PeriodicalIF":0.0,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010500503587","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26048953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Initiation of the reproductive cycle. 生殖周期的开始。

Archives of andrology Pub Date : 2006-07-01 DOI: 10.1080/01485010600666664

B Hafez

引用次数: 0

Increasing expression of GST-pi MIF, and ID1 genes in chemoresistant prostate cancer cells. GST-pi、MIF和ID1基因在化疗耐药前列腺癌细胞中的表达增加。

Archives of andrology Pub Date : 2006-07-01 DOI: 10.1080/01485010600630124

D-S Yu, D S Hsieh, S Y Chang

{"title":"Increasing expression of GST-pi MIF, and ID1 genes in chemoresistant prostate cancer cells.","authors":"D-S Yu, D S Hsieh, S Y Chang","doi":"10.1080/01485010600630124","DOIUrl":"https://doi.org/10.1080/01485010600630124","url":null,"abstract":"The differential expression of genes and related proteins of multidrug resistance in chemoresistant prostate cancer cell lines were elucidated in this study. RNA extracted from doxorubicin-resistant rat prostate cancer (PCa) cells (AT3/ADR1000) and native PCa cells was hybridized to expression arrays containing cDNAs from 588 known genes. Differential expression of selected genes was confirmed by quantitative reverse-transcription polymerase chain reaction (RT-PCR) analysis. Protein contents were measured by fluorescent flow cytometry and immunoblotting. Localization of selected proteins in cells was observed by immunocytochemical staining. Up-regulation of eleven genes and down-regulation of one single gene were displayed in the chemoresistant prostate cancer cells. Overexpression of mRNAs in macrophage migration inhibitory factor (MIF), DNA binding protein inhibitor 1 (ID1), and glutathione S-transferase-pi (GST-pi) were confirmed by gene-specific RT-PCR. Protein over-expression of GST-pi, MIF, and ID1 in resistant cells were 3.3-, 1.5-, and 1.5-fold to native cells, respectively. Immunocytochemistry revealed that GST-pi, MIF, and ID1 were present primarily in the cytoplasm of tumor cells, but ID1 also could be found in the nucleus. AT3/ADR1000 drug-resistant PCa cells displayed significantly increased expression of GST-pi, MIF, and ID1 proteins when compared with native PCa cells. It indicates these genes may play a role in drug resistance of prostate cancer.","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"52 4","pages":"275-81"},"PeriodicalIF":0.0,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010600630124","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26048951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

The genetics of male infertility: a field of study whose time is now. 男性不育的遗传学:一个研究领域，现在是时候了。

Archives of andrology Pub Date : 2006-07-01 DOI: 10.1080/01485010500503603

D T Carrell, C De Jonge, D J Lamb

引用次数: 61

New hope for infertility therapy: fabricating gametes from stem cells. 不孕症治疗的新希望:用干细胞制造配子。

Archives of andrology Pub Date : 2006-07-01 DOI: 10.1080/01485010500431128

H L Feng, Y B Han, A Hershlag, H Yang

引用次数: 2

Interstitial cells of Cajal in erectile dysfunction. Cajal间质细胞与勃起功能障碍的关系。

Archives of andrology Pub Date : 2006-07-01 DOI: 10.1080/01485010500431110

A Shafik, I Shafik, A A Shafik, O El-Sibai

{"title":"Interstitial cells of Cajal in erectile dysfunction.","authors":"A Shafik, I Shafik, A A Shafik, O El-Sibai","doi":"10.1080/01485010500431110","DOIUrl":"https://doi.org/10.1080/01485010500431110","url":null,"abstract":"The corpora cavernosa (CC) evokes electric activity. Slow waves (SWs) appear to originate from interstitial cells of Cajal (ICCs), which seem to control the activity of the smooth muscle cells (SMC). The ICCs were demonstrated to exist in the CC. We investigated the hypothesis that the ICC distribution differs with each of the various ED types. The study comprised 62 men with ED: 16 neurogenic (NGED), 15 arteriogenic (AGED), 11 venogenic (VGED) and 22 psychogenic (PGED). 15 volunteers with normal erections acted as controls. The patients underwent a complete diagnostic evaluation. A biopsy of 3 x 3 mm from the CC was subjected to C-kit immunohistochemistry examination. Specificity control of the antisera consisted of incubation of the tissue with normal rabbit serum substituted for the primary antiserum. C-kit positive stellate-appearing cells resembling those of ICC were detected in the controls. The branches were either laterally located (multipolar) or lying at each pole (bipolar). They were distinguishable from the SMC, which were C-kit negative. ICC were detected in all specimens from patients with NGED and VGED, absent in 13/15 with AGED and scanty in PGED. ICC distribution was different in the various types of ED. It is suggested that this distribution interferes with SW discharge and the control of SMC activity with a resulting ED.","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"52 4","pages":"255-62"},"PeriodicalIF":0.0,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010500431110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26048944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Possible therapy of male infertility by reproductive cloning: one cloned human 4-cell embryo. 生殖克隆治疗男性不育症的可能方法:一个克隆的人类4细胞胚胎。

Archives of andrology Pub Date : 2006-07-01 DOI: 10.1080/01485010500503637

P M Zavos, K Illmensee

{"title":"Possible therapy of male infertility by reproductive cloning: one cloned human 4-cell embryo.","authors":"P M Zavos, K Illmensee","doi":"10.1080/01485010500503637","DOIUrl":"https://doi.org/10.1080/01485010500503637","url":null,"abstract":"This study was conducted to evaluate the preimplantation embryonic potential of adult somatic cells from an infertile man using an interspecies bioassay for quality control and also to create human embryos via somatic cell nuclear transfer (SCNT). Skin tissue was biopsied from infertile man to obtain fibroblast cells. These cells were fused with both enucleated bovine oocytes obtained commercially and human oocytes obtained from his wife. SCNT-reconstructed oocytes were cultured in-vitro. Interspecies SCNT embryos were prepared for PCR and DNA analysis. From 13 SCNT-reconstructed bovine oocytes, 7 embryos developed (54%). DNA sequencing of these interspecies embryos showed the presence of human genomic DNA specific for the fibroblast cells of the man. From three SCNT-reconstructed human oocytes, one developed to the 4-cell stage and was subsequently transferred into the patient's uterus. Blood ss-hCG levels showed a negative pregnancy result. Human fibroblast cells from an infertile patient can promote embryonic development in interspecies SCNT. This is the first evidence of the creation and transfer of a human cloned embryo for reproductive purposes. Even though no pregnancy was established, human reproduction via SCNT may be possible and applicable in the future for patients with severe male or female infertility that have no other alternative options for procreating their own offspring.","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"52 4","pages":"243-54"},"PeriodicalIF":0.0,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010500503637","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26049081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Transurethral prostatectomy using Vista bipolar radiofrequency system: comparison with conventional transurethral resection of the prostate. 采用Vista双极射频系统经尿道前列腺切除术:与传统经尿道前列腺切除术的比较。

Archives of andrology Pub Date : 2006-07-01 DOI: 10.1080/01485010500431102

X-D Li, S Cheng, X-F Rui, G-H Li, Y-B Chen, H-Y Wu

{"title":"Transurethral prostatectomy using Vista bipolar radiofrequency system: comparison with conventional transurethral resection of the prostate.","authors":"X-D Li, S Cheng, X-F Rui, G-H Li, Y-B Chen, H-Y Wu","doi":"10.1080/01485010500431102","DOIUrl":"https://doi.org/10.1080/01485010500431102","url":null,"abstract":"To assess the efficacy and safety of transurethral prostatectomy using Vista system, between 2002 and 2004, patients with symptomatic BPH without suspected cancer were treated using the Vista device. The therapeutic effect was retrospective studied compared with patients who were received by TURP. Bipolar resection using the Vista device exhibits a statistically difference in maximum urinary flow rate, RUV, IPSS and QOL(P < .05) 3 and 6 months after operation, and no transurethral resection syndrome occurred. TURP also exhibits a statistically difference in maximum urinary flow rate, RUV, IPSS and QOL(P < .05), but TURS occurred in 2 patients. Compared with TURP, the Vista device shows a statistically less blood loss (P < .05), and longer operation time in prostate enlarged III(0)(P < .05). The Vista system seems to be effective and safe, and especially fit the patients who have a bigger prostate and high risk factors. It appears to be an effective treatment for BPH. Long-term results should be evaluated.","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"52 4","pages":"293-8"},"PeriodicalIF":0.0,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010500431102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26048945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Significance of semen cultures for men with questionable semen quality. 精液培养对精液质量有问题的男性的意义。

Archives of andrology Pub Date : 2006-07-01 DOI: 10.1080/01485010600630132

M Damirayakhian, R S Jeyendran, S A Land

引用次数: 24

Modulation of MDR-1 gene by MIF and GSTpi with drug resistance generation in hormone independent prostate cancer. MIF和GSTpi在激素依赖型前列腺癌耐药产生中的调控作用

Archives of andrology Pub Date : 2006-07-01 DOI: 10.1080/01485010600630116

D-S Yu, J C Lin, D S Hsieh, S Y Chang, C F Lee

{"title":"Modulation of MDR-1 gene by MIF and GSTpi with drug resistance generation in hormone independent prostate cancer.","authors":"D-S Yu, J C Lin, D S Hsieh, S Y Chang, C F Lee","doi":"10.1080/01485010600630116","DOIUrl":"https://doi.org/10.1080/01485010600630116","url":null,"abstract":"The expression of MIF and GSTpi were upregulated in prostate cancer cells with mulitdrug resistant phenotype. The aim of this study is to determine the relationship between these genes and multidrug resistance (mdr-1) gene in acquired multidrug resistance of prostate cancer. The expression of MIF, GSTpi and gp-170 in multidrug resistant (MDR) subline or native cells were determined using flow cytometry and western blotting. The mRNA level of various genes was analyzed with RT-PCR method. The chemosensitivity of tumor cells and stable transfectants to paclitaxel was measured using MTT (tetrazolium bromide) assay. The protein levels of MIF, GSTpi and gp-170 increased in MDR sublines of prostate cancer when compared with their parental cells. The MIF and GSTpi stable transfectants expressed higher MIF and GSTpi protein levels than their parental cells in western blotting analysis, respectively. The expression of mdr-1 gene and the production of pg-170 were also increased in either MIF or GSTpi stable transfectants when compared with vector control by using RT-PCR and flow cytometric analysis. The MTT results demonstrated that the increased chemoresistance was correlated with the increased production of gp-170 protein in either MIF or GSTpi transfectants. The upregulation of MIF and GSTpi during the development of acquired drug resistance of hormone independent prostate cancer may simultaneously and partially modulate the activation of gp-170.","PeriodicalId":8143,"journal":{"name":"Archives of andrology","volume":"52 4","pages":"283-91"},"PeriodicalIF":0.0,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01485010600630116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26049083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6