Aquaculture Research最新文献

{"title":"Anesthetic Efficacy and Physiological Response to MS-222, Eugenol, and Cymbopogon flexuosus Essential Oil in Juvenile Spotted Seatrout (Cynoscion nebulosus)","authors":"Martín Arenas,&nbsp;Alejandra Rosales-Arce,&nbsp;Yemitzi Galan-Conde,&nbsp;Mariana Ghizell Sanchés-Fuentes,&nbsp;Alvaro Barreto,&nbsp;Adriana del Carmen Paredes-Medina,&nbsp;Juan Carlos Maldonado-Flores,&nbsp;Gabriela Gaxiola","doi":"10.1155/are/1376729","DOIUrl":"https://doi.org/10.1155/are/1376729","url":null,"abstract":"<p>For aquaculture, animal welfare constitutes a fundamental dimension of sustainability because it directly influences health, productivity, resource efficiency, and the ethical standards of production systems. Spotted seatrout (<i>Cynoscion nebulosus</i>) is a marine fish recognized as an emerging candidate for aquaculture in the Gulf of Mexico and, like other developing species, is continually subjected to experimental research to support the advancement of its culture technologies. In this context, identifying effective anesthesia conditions is essential for minimizing stress during operational procedures and improving the consistency of experimental outcomes. The aim of this study was to comparatively evaluate anesthetic effectiveness in juvenile spotted seatrout using behavioral responses and blood biochemical stress indicators. The anesthetic effectiveness of MS-222, eugenol, and <i>Cymbopogon flexuosus</i> essential oil (CFEO) was evaluated in juvenile spotted seatrout (mean ± SD: 28.2 ± 3.5 g wet weight and 14.7 ± 0.7 cm total length). MS-222 at 200, 250, and 300 mg L⁻¹, eugenol at 25, 30, 35, and 40 mg L⁻¹, and CFEO at 100 and 125 µL⁻¹ induced deep anesthesia within 15 min, followed by complete recovery within 5 min. However, only MS-222 at 300 mg L⁻¹ and eugenol at 40 mg L⁻¹ achieved deep anesthesia within 3 min, 110.0 and 149.5 s, respectively. Despite this, MS-222 at 300 mg L⁻¹ produced the highest plasma lactate levels, whereas eugenol at 40 mg L⁻¹ elevated both plasma lactate and glucose relative to the control group. In contrast, eugenol at 35 mg L⁻¹ resulted in the lowest plasma lactate and glucose levels. These findings indicate that eugenol at 35 mg L⁻¹ represents a balanced anesthetic option for juvenile spotted seatrout. Moreover, rapid anesthetic induction does not necessarily imply reduced physiological stress in this species.</p>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2026 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/1376729","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Replacing Fish Oil With Modified High-DHA Canola Oil (Aquaterra) in Pacific White Shrimp (Penaeus vannamei) Diets: Effects on Growth Performance and Nutritional Composition","authors":"Vikas Kumar,&nbsp;Krishna Pada Singha,&nbsp;Ali Hamidoghli,&nbsp;Srinu Reddy,&nbsp;Zhuyun June Yao,&nbsp;Brian C. Small","doi":"10.1155/are/7279854","DOIUrl":"https://doi.org/10.1155/are/7279854","url":null,"abstract":"<p>Fish oil (FO) is widely used in aquaculture feed due to its high levels of long-chain omega-3 fatty acids. But increasing cost, limited supply, and sustainability concerns have led to the search for alternative omega-3 sources. Bioengineered (BE) oilseeds, such as docosahexaenoic acid (DHA) canola (<i>Brassica napus</i>, OECD Unique Identifier: NS-B5ØØ27-4, branded Aquaterra), offer a scalable, land-based source of DHA and other long-chain omega-3 fatty acids. This study evaluated the efficacy of DHA canola oil (DCO, Aquaterra), as a replacement for FO in the diet of Pacific white shrimp (<i>Penaeus vannamei</i>). Five isonitrogenous, isolipidic, and isocaloric diets were formulated by replacing FO with graded levels of DCO (0%, 25%, 50%, 75%, and 100%). Postlarval shrimp (initial weight 0.7–0.8 g) were stocked in a recirculating saltwater system (30 ppt) comprised of 40 aquaria (eight tanks for each diet), and shrimp were fed four times daily for 11 weeks. Results showed no significant differences in growth performance or survival across different dietary groups. Feed intake was reduced at 100% FO replacement, but feed conversion ratio (FCR) and energy retention improved. Whole-body protein and energy remained stable across all dietary groups, while lipid retention increased with DCO-fed groups. Whole-body amino acid and fatty acid compositions were influenced by DCO inclusion, with the 50% replacement group exhibiting comparable composition to that of the 100% FO group, especially in terms of the high content of total omega-3 fatty acids. For growth, DCO can replace up to 100% FO in shrimp diets; however, 75% DCO replacement is recommended when considering the whole-body fatty acid profile and <i>n</i>−3/<i>n</i>−6 ratio. Based on the present findings, 50%–75% FO replacement with DCO in shrimp diets can be considered optimal, supporting its use as a robust and viable lipid source for commercial shrimp aquaculture.</p>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2026 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/7279854","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics-Driven Pre-Growout Phenotyping Identifies Candidate Metabolic Signatures Associated With White Shrimp Performance","authors":"Chiu-Hui Kuo,&nbsp;Chen-Hsun Liu,&nbsp;Yang-De Chen,&nbsp;Feng-Cheng Wu,&nbsp;Kuohsun Chiu","doi":"10.1155/are/7396346","DOIUrl":"https://doi.org/10.1155/are/7396346","url":null,"abstract":"<p>Selective breeding has improved the performance of Pacific white shrimp (<i>Penaeus vannamei</i>), yet routine selection still relies mainly on late-stage phenotypes that are costly to measure and sensitive to fluctuating pond environments. Here, we tested whether early-life hepatopancreas metabolomes can capture line-associated physiological differences relevant to production. Postlarvae at day 40 (PL40) from four breeding lines (designated as TK1–TK4) were profiled using an UHPLC–HRMS-based untargeted metabolomics workflow in positive-ion mode. Metabolomic signatures were related to a matched 60-day grow-out evaluation, in which TK3 achieved the highest final weight (15.80 ± 1.20 g) and the lowest observed feed conversion ratio (FCR) (1.50 ± 0.07), whereas survival varied substantially across lines (54.1%–89.5%), with TK3 and TK4 showing higher survival than TK1 and TK2. Principal component analysis (PCA) revealed line-dependent clustering, and pairwise orthogonal partial least squares–discriminant analysis (OPLS-DA) models supported exploratory discrimination of TK3 from each comparator line. Differential metabolite and pathway analyses implicated coordinated variation in amino acid and nitrogen handling, nucleotide turnover, and lipid utilization, with recurring enrichment of histidine metabolism, lysine degradation, purine metabolism, and glutathione-related processes. Receiver operating characteristic (ROC)-based prioritization further highlighted a subset of candidate metabolites for follow-up targeted validation. Given the limited metabolomics cohort size, these findings should be interpreted as exploratory discovery-stage signatures rather than validated biomarkers. By leveraging known grow-out divergence among breeding lines, this study adopted a performance-anchored retrospective design to nominate PL40 metabolic pathways and candidate metabolites that may support future pre-growout screening in shrimp improvement programs after independent validation.</p>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2026 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/7396346","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marking Otoliths of Adult European flounder (Platichthys flesus Linnaeus, 1758) With Tetracycline and Calcein","authors":"Hussaini O. Adamu,&nbsp;Kate McQueen,&nbsp;Steffi Meyer,&nbsp;Uwe Krumme","doi":"10.1155/are/5022643","DOIUrl":"https://doi.org/10.1155/are/5022643","url":null,"abstract":"<p>Mark-recapture studies using fluorochrome dyes are a valuable approach for validating the age determination of fish, yet the optimal chemical marker and its dosage for adult European flounder (<i>Platichthys flesus</i> Linnaeus, 1758) remain unclear. We assessed the effectiveness of tetracycline hydrochloride and calcein at different concentrations for marking otoliths and the survival rates. A total of 117 adult European flounder were injected intraperitoneally either with one of the chemical markers or with a saline solution as a control. They were held in stationary net pens in the western Baltic Sea over a 69-day period. Fish were monitored for survival, growth, and body condition, and otoliths were examined for fluorescence mark quality. Survival was highest in the tetracycline groups: 90% at 25 and 50 mg/kg, and 82% at 100 mg/kg, whereas calcein treatments were associated with intermediate survival: 55% at 0.5 mg/kg and 50% at 5 mg/kg. A total of 76% of otoliths in the tetracycline hydrochloride-treated fish showed usable fluorescence marks (good: 12%, poor: 64%), while marks of calcein-treated fish were only observed in 10% of the otoliths. The differences in survival outcome between the three tetracycline dosages were not significant, supporting the use of the lowest tested tetracycline dose (25 mg/kg) for effective marking. These results indicate that tetracycline hydrochloride is a suitable and well-tolerated chemical marker for adult European flounder, whereas calcein was unsuitable due to the recorded mortality and low marking success. The findings provide a methodological basis for mark-recapture studies of European flounder in the Baltic Sea and may inform similar applications in other areas and in related flatfish species.</p>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2026 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/5022643","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the Trophic Index (TRIX) of Sea-Cage Aquaculture Farms in the Southern Black Sea: Influence of Water-Column Depth Gradients","authors":"Birol Baki,&nbsp;Oylum Gökkurt Baki","doi":"10.1155/are/6209531","DOIUrl":"https://doi.org/10.1155/are/6209531","url":null,"abstract":"<p>Marine sea-cage aquaculture continues to expand, increasing the need for monitoring tools capable of distinguishing farm-related nutrient enrichment from natural coastal variability. This study evaluated the trophic status of 18 sea-cage aquaculture farms along the southern Black Sea coast using the Trophic Index (TRIX), which integrates chlorophyl-a, oxygen saturation deviation, dissolved inorganic nitrogen, and total phosphorus. A nested hierarchical sampling design was used to compare cage and reference site types across three depth strata (surface, midwater, and bottom). A total of 131 TRIX observations were analyzed using linear mixed-effects models, with farm identity included as a random intercept to distinguish cage-proximity effects from farm-level background conditions. TRIX values fell predominantly within the good (4–&lt;5) to moderate (5–&lt;6) trophic classes, with the highest mean values observed in Zone 3 during spring. Model-adjusted means were similar between site types (reference: 4.98; cage: 4.89), indicating no systematic evidence of water-column enrichment associated with cage proximity at the examined spatial scales. In contrast, trophic conditions exhibited significant vertical structuring. Variance partitioning showed that fixed effects explained 29.6% of TRIX variability (<i>R</i>²<i>m</i> = 0.296), whereas the full model explained 66.1% (<i>R</i>²<i>c</i> = 0.661), with substantial among-farm heterogeneity (ICC = 0.518). Multivariate ordination further indicated a strong overlap between the cage and reference samples, with the first two PCA axes explaining 60.0% of the total variance. In sum, these findings suggest that the pelagic trophic variability in this region is governed primarily by bathymetry and local hydrographic context, supporting the spatial planning strategies that prioritize deeper, well-flushed sites.</p>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2026 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6209531","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148282663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How Dietary Methionine Shields Golden Pompano (Trachinotus ovatus) From Cold-Stress Triads via Nrf2 Activation?","authors":"Bissih Fred,&nbsp;Chunfeng Yao,&nbsp;Agyenim Godfred Boateng,&nbsp;Jiacheng Zhang,&nbsp;Beiping Tan,&nbsp;Asare Derrick,&nbsp;Fomekong Fomekong Rachel Merveille,&nbsp;Kaimin Cheng,&nbsp;Shuyan Chi","doi":"10.1155/are/5296072","DOIUrl":"https://doi.org/10.1155/are/5296072","url":null,"abstract":"<p>This study evaluated the physiological and molecular mechanisms underlying cold stress tolerance in golden pompano (<i>Trachinotus ovatus</i>) after a 56-day feeding trial with high-lipid diets (18%) supplemented with varying methionine (Met) levels (1.04%, 1.14%, 1.24%, 1.34%, 1.44%, 1.54%, and 1.64%). After the feeding period, fish fed 1.24% Met (HLM3) exhibited the highest thermal growth coefficient (TGC) and relative growth rate (RGR), together with the lowest feed conversion ratio (FCR) among all treatments (<i>p</i> &lt; 0.05). Survival remained 100% across all dietary groups throughout the feeding period. Afterwards, 10 fish from each cage selected randomly were subjected to a 96-h cold stress from 19.30 ± 0.06°C to 14.38 ± 0.01°C to assess survival, lipid metabolism, antioxidant capacity, immune function, ferroptosis, inflammation, and apoptosis. <i>T. ovatus</i> in the HLM3 group exhibited significantly reduced serum levels of total cholesterol, triglycerides, LDL-C, AST, ALT, and NEFAs, accompanied by elevated antioxidant enzyme activities (SOD, CAT, T-AOC, and GSH-Px) and lower MDA and ROS levels (<i>p</i> &lt; 0.05). Immune parameters (IgM, LZM, ACP, and AKP) were also markedly enhanced in HLM3. Moreover, hepatic Met and cysteine levels were higher in HLM3, while hepatic lipid deposition was suppressed. Lipolytic (<i>cpt1a</i>, <i>atgl</i>, <i>lpl</i>, and <i>pparα</i>) and antioxidant genes (<i>nrf2</i>, <i>ho-1</i>, <i>gxp4</i>, and <i>MnSOD</i>) were upregulated, along with increased NADP⁺/NADPH ratio and downregulation of <i>keap1</i>. <i>S lc7a11</i>, <i>bcl2</i>, and <i>bcl-xl</i> genes were elevated, while ferroptosis and apoptosis markers were alleviated. Overall, dietary inclusion of 1.24% Met in an 18% high-lipid diet enhanced the <i>nrf2</i>-mediated antioxidant defense, lipid catabolism, and antiferroptotic pathways, thereby improving cold stress resilience in <i>T. ovatus</i>.</p>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2026 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/5296072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148282338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Demand Feeders Provide a Direct Palatability Comparison for Assessing Aquaculture Diets on Cultured Yellowtail Kingfish, Seriola lalandi","authors":"Connor Paduano,&nbsp;Gavin J. Partridge,&nbsp;Benjamin J. Saunders,&nbsp;Lindsey Woolley","doi":"10.1155/are/8795431","DOIUrl":"https://doi.org/10.1155/are/8795431","url":null,"abstract":"<p>This study demonstrates that demand feeders are a viable palatability tool to assess feeding preferences of yellowtail kingfish (<i>Seriola lalandi</i>) (YTK). In two trials, juvenile YTK were offered three diets simultaneously using separate demand feeders to compare diet palatability and preference. Trial 1 compared the consumption of two commercial diets (commercial diets A and B) against a negative control to determine whether YTK could operate demand feeders and exhibit a preference for specific diets. Trial 2 compared the consumption of commercial diet A against two diets containing alternative protein and lipid sources to replace marine ingredients, referred to as reduced marine ingredient (RMI) diets X and Y. During Trial 1, juvenile YTK showed a preference for commercial diet B, followed by commercial diet A, and least preferred the negative control. The higher consumption of commercial diet B and the extremely low consumption of the negative control demonstrate that YTK can both learn to operate demand feeders and exhibit feed preferences when offered multiple diets simultaneously. In Trial 2, there was no preference for any diet offered, suggesting there were no palatability issues with the RMI diets. This study demonstrated that demand feeders are a viable tool to assess feed preferences and palatability of aquaculture diets for YTK, although waste resulting from feeding behaviour negatively impacted the feed conversion ratio (FCR) achieved.</p>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2026 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/8795431","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148282337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aeration Unexpectedly Increases Ventilation Rates of Farmed Atlantic Salmon (Salmo salar) in a River Environment During High Temperatures","authors":"Quynh Le Khanh Vo,&nbsp;Kylie A. Pitt,&nbsp;Bela Stantic,&nbsp;Tyler Spencer,&nbsp;Christine Huynh,&nbsp;Lukas Folkman","doi":"10.1155/are/9899740","DOIUrl":"https://doi.org/10.1155/are/9899740","url":null,"abstract":"<p>Aeration is commonly used in commercial salmon aquaculture to improve water quality and fish welfare, but it also represents a significant operational cost. Its effects on fish behaviour and welfare, therefore, require evaluation. We used computer vision and a manipulative experiment to test whether salmon exposed to aeration reduced their ventilation effort (fewer open-close mouth cycles) compared to non-aerated conditions. Twenty-eight underwater videos (14 pairs) were recorded across multiple pens with aeration on and off during the low slack water. A computer vision model detected, classified and tracked mouth opening and closing cycles per minute as a proxy for ventilation rate. Linear mixed-effects analysis found a positive association between ventilation rates and aeration operations, likely reflecting a behavioural response to water turbulence and bubble flow rather than changes in dissolved oxygen (DO) or temperature. These findings highlight the utility of computer vision for monitoring ventilation responses under commercial conditions.</p>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2026 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/9899740","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148281894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Musculoskeletal Assessment of Rainbow Trout (Oncorhynchus mykiss) Reared in a Photoelectrocatalysis (PEC)-Based Recirculating Aquaculture System","authors":"G. Mirra,&nbsp;E. Buoio,&nbsp;L. Maistrello,&nbsp;C. Cialini,&nbsp;G. L. Chiarello,&nbsp;A. Costa,&nbsp;C. Bazzocchi,&nbsp;E. Mainardi,&nbsp;R. Rossi,&nbsp;N. Cherif,&nbsp;T. Temraz,&nbsp;D. Bertotto,&nbsp;E. Fonsatti,&nbsp;M. Pallaoro,&nbsp;S. Modina,&nbsp;M. Di Giancamillo,&nbsp;A. Di Giancamillo,&nbsp;L. Aidos","doi":"10.1155/are/3037374","DOIUrl":"https://doi.org/10.1155/are/3037374","url":null,"abstract":"<p>This study explored the innovative application of photoelectrocatalysis (PEC), an advanced evolution of conventional photocatalysis, as a water remediation technology in recirculating aquaculture systems (RASs) for adult rainbow trout (<i>Oncorhynchus mykiss</i>, 150–200 g). The research focused on its impact on fish muscle and skeletal health using a multidisciplinary approach. Water quality parameters were registered and monitored, including nitrate, nitrite, and ammonia concentrations, and calcium content was measured at the end of the 1-month trial. To identify potential skeletal anomalies, imaging analyses including X-ray and computed tomography (CT) were performed. Additionally, histochemical analysis with Picrosirius Red (PSR) staining was used to evaluate collagen distribution in the vertebrae. Muscle density was assessed by CT scans and histological analysis to study metabolism and the size/diameter of white muscle fibers. Finally, genes related to myogenesis and antioxidant enzymes were studied to detect muscle growth and oxidative stress, respectively. The PEC showed lower nitrite and nitrate levels and higher dissolved oxygen (DO) compared to the control (CTR), likely due to photoelectrocatalytic activity, with overall more stable water chemistry. At the end of the trial, a reduction in calcium content in the water was observed (<i>p</i>  &lt; 0.05), without affecting the calcium content in the vertebral bones. Skeletal analyses revealed no anomalies, and collagen distribution in the bone matrix appeared normal. Muscle morphology indicated proper development, with a slight hypertrophic trend in the PEC group. Oxidative stress analysis suggested no adverse effects from PEC treatment, as antioxidant enzyme levels were similar between groups. In summary, the absence of skeletal anomalies and adequate muscle growth, associated with the improvement of water quality in the PEC, suggests that this new remediation technology is a valid, sustainable aquaculture approach, combining environmental innovation and fish health.</p>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2026 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/3037374","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148281962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a Plant Protein-Based Diet on the Somatic Growth and Transcriptional Response of Oysters","authors":"Yulong Yao,&nbsp;Haoran Liu,&nbsp;Mingkun Liu,&nbsp;Luping Wang,&nbsp;Shentong Wang,&nbsp;Lintao Tan,&nbsp;Chi Yu,&nbsp;Haigang Qi,&nbsp;Li Li,&nbsp;Guofan Zhang","doi":"10.1155/are/2254938","DOIUrl":"https://doi.org/10.1155/are/2254938","url":null,"abstract":"<p>Food scarcity often leads to emaciation in offshore-farmed oysters, thereby diminishing their marketability. Land-based intensive fattening is an emerging strategy to improve oyster somatic growth, yet its broad implementation is constrained by the scarcity of effective and stable feed sources. In this study, the fattening performance of a formulated diet based on fermented plant proteins (soybean and peanut meal) with minimal microalgae supplementation (diet group) was evaluated against a microalgae-only control (control group) during a 10-day intensive trial in Pacific oysters (<i>Magallana gigas</i>). In parallel, transcriptomic analysis was conducted to elucidate the underlying mechanisms of plant protein utilization. Relative to the initial state (Day 0), the diet group showed significant improvements in meat yield, condition index, dry flesh weight, and the contents of protein, glycogen, and lipid. By Day 10, the diet group exhibited significantly higher condition index, dry flesh weight, and protein content compared with the control group, whereas the glycogen content was significantly lower, indicating a distinct nutrient accumulation pattern. Enzyme activity assays revealed high protease but low amylase and lipase activities in the diet group, confirming preferential protein assimilation. Principal component analysis (PCA) of the transcriptomic profiles revealed a distinct separation between the diet and control groups. Functional enrichment and gene expression analysis indicated an enhancement of aminoacyl-tRNA (aa-tRNA) biosynthesis and mTOR-mediated protein synthesis in the diet group, accompanied by a significant reduction of carbohydrate metabolism, including glycolysis, the TCA cycle, and oxidative phosphorylation. These results illustrated that plant protein improved oyster biomass accumulation via mTOR-mediated protein synthesis, while underscoring the need for balanced carbohydrate and lipid supplementation to optimize nutritional outcomes. This study provides a practical framework for land-based oyster fattening and informs future nutritional strategies for bivalve aquaculture.</p>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2026 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/2254938","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}