Annals of Clinical Microbiology and Antimicrobials最新文献

{"title":"Identification and preclinical efficacy evaluation of two lytic bacteriophages targeting highly virulent and multidrug-resistant Klebsiella pneumoniae.","authors":"Ai Guo, Dianbao Zuo, Li Shi, Ming Guo, Jinquan Li, Caili Li, Puqing Wang, Xiaodong Sun, Ming Sang","doi":"10.1186/s12941-025-00812-9","DOIUrl":"https://doi.org/10.1186/s12941-025-00812-9","url":null,"abstract":"<p><strong>Background: </strong>The emergence of MDR K. pneumoniae poses a critical challenge in treating respiratory-associated pneumonia. Bacteriophages are promising antibiotic alternatives with unique features. This study aimed to isolate new bacteriophages from the hospital environment and investigate their therapeutic potential and mechanisms.</p><p><strong>Methods: </strong>We employed plaque assays, transmission electron microscopy, and whole-genome sequencing to systematically characterize the biological properties, morphology, and genomic profiles of the phages in parallel. The bacteriostatic curve, biofilm staining quantification, and biofilm inhibition rate assay were employed to evaluate the in vitro lytic efficacy of the phage. More importantly, we established the murine pneumonia infection models through nasal instillation, assessed the therapeutic potential of the phage in vivo by observing pathological morphology via HE staining, detecting pro-inflammatory cytokine levels via qPCR and ELISA, and monitoring bacterial load changes in lung tissue through PCR analysis.</p><p><strong>Results: </strong>Phages vB_KpnP_XY3 and vB_KpnP_XY4, taxonomically classified as Siphoviridae, demonstrated broad temperature (4-60 °C), pH (4-11) tolerance, chloroform resistance, latent periods of 40/35 min, and burst sizes of 340/126 PFU/cell. Both genomes contained circular dsDNA genomes (47,466 bp/50,036 bp) without virulence or antibiotic resistance genes. The bacterial concentration markedly decreased at 2 h post-treatment, reaching its biological nadir by 6 h. Concurrent biofilm assays demonstrated 80% biofilm inhibition and rapid bacterial clearance. In murine pneumonia models, both phage monotherapy and phage-antibiotic combinations significantly reduced bacterial loads compared with antibiotics alone (P < 0.05), concurrently attenuating inflammation (IL-1β/IL-6/TNF-a. P < 0.0001) and restoring alveolar architecture with reduced necrosis.</p><p><strong>Conclusion: </strong>The phages vB_KpnP_XY3 and vB_KpnP_XY4 demonstrated robust environmental adaptability. Its antibacterial effect is related to its specific biofilm dissolution performance in vivo and in vitro. These findings provide strong evidence for the precise phage treatment of MDR K. pneumoniae infections.</p>","PeriodicalId":8052,"journal":{"name":"Annals of Clinical Microbiology and Antimicrobials","volume":"24 1","pages":"46"},"PeriodicalIF":3.6,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome recombination and dynamic accessory genomes drive the phenotypic diversity of Mycobacterium abscessus subspecies.","authors":"Yu Chen, Rong Bao, Na Li, Tingting Fang, Xiaoyu Yin, Le Qin, Bijie Hu, Qing Miao","doi":"10.1186/s12941-025-00804-9","DOIUrl":"10.1186/s12941-025-00804-9","url":null,"abstract":"<p><strong>Background: </strong>Mycobacterium abscessus (Mab) is a multidrug-resistant bacterial pathogen capable of causing widespread infections, often with a poor prognosis in susceptible populations. Mab comprises three distinct subspecies that exhibit phenotypic diversity and genetic heterogeneity.</p><p><strong>Methods: </strong>We performed whole-genome sequencing and phenotypic antimicrobial susceptibility testing on 109 Mab isolates collected at zhongshan hospital from 2018 to 2023.</p><p><strong>Results: </strong>The results indicate that recombination, especially distributed conjugation transfer, promotes the formation and sustained diversity of Mab subspecies. Through pangenome analysis, the synergistic gain/loss of accessory genes was found to contribute to different metabolic profiles and the ability to adapt to oxidative stress, facilitating strain adaptation to host environments. We conducted phenotypic antimicrobial susceptibility testing, revealing resistance to macrolide antibiotics differed among subspecies. We identified 24 genes whose gain or loss may increase the likelihood of macrolide resistance, including those involved in biofilm formation, the stress response, virulence, biotin synthesis, and fatty acid metabolism. Genomic variations within Mab species may have significant implications for disease epidemiology, infection pathogenesis, and host interactions.</p><p><strong>Conclusions: </strong>Our findings provide a valuable genetic basis for the success of Mab as a highly adaptive and drug-resistant pathogen, informing current efforts to control and treat Mab infections, including strategies targeting specific sequence types or lineages.</p>","PeriodicalId":8052,"journal":{"name":"Annals of Clinical Microbiology and Antimicrobials","volume":"24 1","pages":"44"},"PeriodicalIF":3.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12285043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriological profile and antimicrobial resistance in sepsis cases in intensive care units in Lubumbashi: challenges and perspectives.","authors":"Michel Muteya Manika, Aristophane Koffi Tano, Liévin Kalala Kapend'a, Floreance Mutomb Mujing'a, Christian Ngama Kakisingi, Serge Kapend Matanda, Ildéphonse Wa Mwanza Teta, Yves Banza Mukalay, Eric Ilunga Kasamba, Berthe Nsimire Barhayiga, Claude Mulumba Mwamba, Albert Tambwe A Nkoy Mwembo, Hippolyte Nani-Tuma Situakibanza, Rivain Fefe Iteke","doi":"10.1186/s12941-025-00811-w","DOIUrl":"10.1186/s12941-025-00811-w","url":null,"abstract":"<p><strong>Background: </strong>Sepsis remains a major public health challenge, leading to high mortality and morbidity rates, particularly among low-income populations such as those in sub-Saharan Africa. Its management is complicated by the emergence of multidrug-resistant bacterial strains, necessitating microbiological surveillance and adaptation of antibiotic therapy. This study examines the microbiological profile of sepsis and the antibiotic susceptibility of pathogens among critically ill patients in Lubumbashi, Democratic Republic of Congo.</p><p><strong>Methods: </strong>A prospective study was conducted from January 2021 to December 2023 across three ICU units in Lubumbashi. Patients suspected of having sepsis were included, and microbiological samples were collected from various sources (blood, urine, pus, biological fluids). Bacterial identification and antibiotic susceptibility testing were performed according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Data were analyzed using SPSS version 23^® and Excel 365^®.</p><p><strong>Results: </strong>Among the 76 patients included, 40% had confirmed bacterial sepsis. The predominant isolates were Gram-negative bacilli (62.7%), with Escherichia coli (28.35%) and Klebsiella pneumoniae (22.73%) being the most common species. Gram-positive bacteria accounted for 33.89%, primarily coagulase-negative streptococci (15.11%) and Enterococcus faecium (5.61%). Antimicrobial resistance profiles revealed a high level of resistance to commonly used antibiotics, particularly cephalosporins, fluoroquinolones, and cotrimoxazole. However, greater sensitivity was observed with amikacin (41.3%), fosfomycin (37%), and meropenem (33.8%).</p><p><strong>Conclusion: </strong>This study highlights the high prevalence of Gram-negative bacteria and concerning resistance to first-line antibiotics, jeopardizing the effectiveness of empirical treatments. These findings underscore the urgency of strengthening microbiological surveillance, rationalizing antibiotic use, and implementing antimicrobial resistance control policies in the DRC. Developing treatment protocols tailored to local data and enforcing stricter antibiotic regulations are essential to improving sepsis management and reducing associated mortality.</p>","PeriodicalId":8052,"journal":{"name":"Annals of Clinical Microbiology and Antimicrobials","volume":"24 1","pages":"42"},"PeriodicalIF":4.6,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of Neisseria meningitidis isolated from patients with urogenital infection in a region of China.","authors":"Qinghui Xie, Yang Yang, Wenwen Xu, Dandan Yang, Jingrui Li, Yijie Tang, Lingyun Shen, Fangyuan Yu, Wenhao Weng, Fuquan Long, Qingqiong Luo","doi":"10.1186/s12941-025-00810-x","DOIUrl":"10.1186/s12941-025-00810-x","url":null,"abstract":"<p><strong>Background: </strong>Neisseria meningitidis (Nm), traditionally recognized as a nasopharyngeal commensal causing invasive meningococcal disease (IMD), has recently emerged as an etiological agent of urethritis worldwide, with sporadic urogenital cases in China raising epidemiological concerns.</p><p><strong>Methods: </strong>Three urogenital Nm isolates were characterized to investigate their evolutionary features and transmission patterns. Through comprehensive laboratory characterization encompassing culture identification (Gram staining, oxidase testing, MALDI-TOF MS), antimicrobial susceptibility profiling, whole-genome sequencing, and functional colonization assays on urethral epithelial cells under nitrite-supplemented microaerobic conditions, three multidrug-resistant Nm isolates were identified.</p><p><strong>Results: </strong>All isolates demonstrated resistance to penicillin and sulfamethoxazole/trimethoprim, with isolate 24-SHSP-NM2 exhibiting additional ciprofloxacin resistance. The resistance was attributed to penA variants, mtrR promoter mutations, and gyrA substitutions. Phylogenetically, one isolate clustered with Japanese ST-11,026 strains and 2 clustered with Australian ST-1466 strains. Genomic characterization identified complete denitrification operons (aniA-norB) in all three isolates, which enable nitrite-enhanced epithelial colonization. ST-1466 isolates showed meningococcal B (MenB) vaccine component FHbp antigenic homology through FHbp variant 1.1.</p><p><strong>Conclusions: </strong>These findings collectively demonstrate the convergent evolution of urogenital tropism, antimicrobial resistance (AMR) emergence, and metabolic adaptation to genitourinary microenvironments, underscoring the threat of genitourinary Nm infections. The study highlights the critical need to enhance molecular surveillance, implement rapid AMR screening, and prioritize MenB vaccination strategies in high-risk populations.</p>","PeriodicalId":8052,"journal":{"name":"Annals of Clinical Microbiology and Antimicrobials","volume":"24 1","pages":"43"},"PeriodicalIF":4.6,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Long COVID clinical evaluation, research and impact on society: a global expert consensus.","authors":"Andrew G Ewing, David Joffe, Svetlana Blitshteyn, Anna E S Brooks, Julien Wist, Yaneer Bar-Yam, Stephane Bilodeau, Jennifer Curtin, Rae Duncan, Mark Faghy, Leo Galland, Etheresia Pretorius, Spela Salamon, Danilo Buonsenso, Claire Hastie, Binita Kane, M Asad Khan, Amos Lal, Dennis Lau, Raina MacIntyre, Sammie McFarland, Daniel Munblit, Jeremy Nicholson, Hanna M Ollila, David Putrino, Alberto Rosario, Timothy Tan","doi":"10.1186/s12941-025-00803-w","DOIUrl":"10.1186/s12941-025-00803-w","url":null,"abstract":"","PeriodicalId":8052,"journal":{"name":"Annals of Clinical Microbiology and Antimicrobials","volume":"24 1","pages":"41"},"PeriodicalIF":4.6,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12243415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbapenem-resistant Klebsiella pneumoniae gut colonization and subsequent infection in pediatric intensive care units in shanghai, China.","authors":"Hongyan Guan, Jingxian Liu, Jiajia Yu, Kanglin Guo, Feng Chen, Jing Yu, Ying Liu","doi":"10.1186/s12941-025-00808-5","DOIUrl":"10.1186/s12941-025-00808-5","url":null,"abstract":"<p><strong>Background: </strong>It has been revealed that carbapenem-resistant Klebsiella pneumoniae (CRKP) colonization is closely associated with subsequent clinical infections. This study aimed to investigate the resistance and epidemiology of CRKP isolated from anal swabs and subsequent clinical infection specimens in two pediatric intensive care unit (ICU) departments. Clinical characteristics were analyzed to identify the risk factors of CRKP infection.</p><p><strong>Methods: </strong>A 3-year retrospective study was carried out in pediatric intensive care units (PICU) and neonatal intensive care units (NICU). CRKP isolates from colonization and infection samples were characterized by testing resistance genes and multilocus sequence typing (MLST). The results of MLST were analyzed to derive CCs by Bionumeric 8.0. Clinical variables such as gestational age, birth weight, mode of delivery, underlying diseases, exposure of antimicrobial agents, history of surgery, length of hospital stay, and prognosis were collected through the electronic medical record system and analyzed by SPSS 22.0.</p><p><strong>Results: </strong>Of the 2225 patients who were screened for CRE colonization, 7.42% of patients were detected positive. The incidence of subsequent infection was 18.18%. Carbapenemase genes bla_KPC-2 and bla_NDM-1 were the most prevalent in the colonization and infection of CRKP. The majority of CRKP isolated from anal swabs and infection samples belonged to CC11/ST11. The distribution of CC11 in the PICU was significantly higher than in NICU. ST11/bla_KPC-2 was significantly higher in infection CRKP isolates. Age older than one year and usage of carbapenems within 3 months prior to detection of CRKP colonization were independent risk factors for CRKP clinical infection.</p><p><strong>Conclusion: </strong>The main prevalence of CRKP varies in different departments. Colonization of ST11/bla_KPC-2 CRKP may increase the incidence of subsequent infections in pediatric ICU patients. Age and usage of carbapenems could increase the risk of CRKP infection in this study.</p>","PeriodicalId":8052,"journal":{"name":"Annals of Clinical Microbiology and Antimicrobials","volume":"24 1","pages":"39"},"PeriodicalIF":4.6,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12225127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pan-drug, colistin, streptomycin, erythromycin, clindamycin resistant Salmonella enterica serovars isolated from slaughtered cattle and human in mansoura, Egypt.","authors":"Shimaa El Baz, Hanan Ahmed Zaher, Wafaa Ragab","doi":"10.1186/s12941-025-00809-4","DOIUrl":"10.1186/s12941-025-00809-4","url":null,"abstract":"<p><strong>Objectives: </strong>Salmonella is recognized globally as a significant foodborne pathogen associated with foodborne outbreaks in both humans and animal. The rise of multidrug-resistant (MDR) Salmonella isolates poses a critical public health challenge. Given that the isolation of Salmonella within abattoirs is a prominent source of community infection especially through the consumption of contaminated meat. This study aims to determine the prevalence of Salmonella, the occurrence of virulence genes (invA, spvC), and specific resistance genes (tetA, sul1, aadA1, aac(3)- IV) in Salmonella isolates isolated from cattle in abattoirs. Additionally, the investigation assesses the potential exposure risks for abattoir workers in Mansoura City, Egypt.</p><p><strong>Methods: </strong>In a study conducted from May to July 2024, a total of 150 samples were collected to investigate the presence of Salmonella in healthy Egyptian Baladi cattle and abattoir workers at the Mansoura abattoir, Mansoura City, Egypt. The sample collection comprised rectal swabs (n = 50) and meat swabs (n = 50) from cattle, in addition to 50 hand swabs obtained from abattoir workers. Salmonella isolation was done following standard microbiological techniques. Initially, pre-enrichment of the samples was conducted using buffered peptone water. Subsequently, selective enrichment was executed using Rappaport Vassiliadis broth, followed by cultivation on xylose-lysine-deoxycholate (XLD) agar to isolate suspected Salmonella colonies. These colonies were then subjected to a series of identification tests, including biochemical assays, slide agglutination tests, and polymerase chain reaction (PCR) targeting the invA gene, which is indicative of Salmonella presence. Furthermore, molecularly identified isolates were tested for the virulence gene spvC, which is related to the pathogenicity of Salmonella. The antimicrobial susceptibility of the isolates was assessed using the Kirby-Bauer disc diffusion method, providing insight into the resistance profiles of the observed isolates. In addition, a subset of 19 Salmonella isolates underwent multiplex PCR analysis to evaluate the presence of specific resistance genes: tetA, sul1, aadA1, and aac(3)-IV.</p><p><strong>Results: </strong>The overall occurrence of Salmonella isolates across all examined samples was 12.7%. This included 4% from cattle carcass swabs, 12% from rectal swabs, and a notable 22% from workers' hands. The most prevalent serotypes identified were Salmonella Enteritidis and Salmonella Typhimurium, exhibiting incidences of 26.3% (n = 5) and 21% (n = 4), respectively. Other serotypes included Salmonella Infantis at 15.8% (n = 3), Salmonella Kentucky and Salmonella Tsevie each at 10.5% (n = 2), and Salmonella Paratyphi A, Salmonella Haifa, and Salmonella Virchow at 5.3% ((n = 1) each). From the tested Salmonella isolates, 100% (19/19) were positive for the invA and 89.5% (17/19) carried Spvc genes. Resistance profiling ca","PeriodicalId":8052,"journal":{"name":"Annals of Clinical Microbiology and Antimicrobials","volume":"24 1","pages":"40"},"PeriodicalIF":3.6,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SARS-CoV-2 coinfection in patients with invasive pulmonary aspergillosis: clinical characteristics and prognosis.","authors":"Mengshu Xie, Xiaofeng Zhu, Ao Ma, Jiaqi Fan, Guangru Fei, Qianqian Zhou, Yan Zhang, Huimei Wu, Xuqin Jiang","doi":"10.1186/s12941-025-00805-8","DOIUrl":"10.1186/s12941-025-00805-8","url":null,"abstract":"<p><strong>Background: </strong>COVID-19 associated pulmonary aspergillosis (CAPA) has been globally reported to be a life-threatening complication of severe COVID-19. Previous studies primarily focused on an association between secondary Aspergillus infection and elevated mortality risk in COVID-19 patients, while potential confounding factors and alternative pathogenic mechanisms remain insufficiently investigated. The risk factors and outcomes of patients with secondary SARS-CoV-2 infection following invasive pulmonary aspergillosis (IPA) were not been well explored either.</p><p><strong>Methods: </strong>This retrospective monocentric study enrolled 152 hospitalized IPA patients with and without SARS-CoV-2 infection from 1 November 2022 to 31 October 2023. The characteristics of IPA patients and related risk factors were investigated, and the relationship between different SARS-CoV-2 infection status and the prognosis in IPA patients was further evaluated.</p><p><strong>Results: </strong>Our analysis demonstrated that IPA patients subsequently diagnosed with SARS-CoV-2 infection exhibited significantly elevated mortality risk compared to those without viral coinfection (53.6% vs. 22.9%, P < 0.001). SARS-CoV-2 infection status (OR 3.708; P = 0.001; 95%CI 1.674-8.212), albumin concentration (OR 0.885; P = 0.005; 95%CI 0.813-0.964), and C-reactive protein level (OR 1.007; P = 0.012; 95%CI 1.002-1.013) were statistically significant independent risk factors for prognosis of IPA patients. Subsequent analysis established a multivariate risk prediction model incorporating independent prognostic factors, which exhibited robust discriminative capacity for mortality risk stratification via ROC curve validation (AUC = 0.792, 95%CI 0.721-0.862, P < 0.0001). A statistically significant difference in mortality rate existed between IPA patients with secondary SARS-CoV-2 infection and CAPA patients (63.2% and 33.3%, P = 0.037). Notably, comparative analysis revealed no statistically significant differences in 28-day (22/96, 22.9% vs. 6/18, 33.3%) or 90-day mortality rates (22/96, 22.9% vs. 6/18, 33.3%) between patients with IPA without SARS-CoV-2 infection and IPA patients with secondary SARS-CoV-2 infection.</p><p><strong>Conclusions: </strong>IPA patients with secondary SARS-CoV-2 coinfection had a lower mortality compared to those with CAPA. Considering the high mortality rate, more medical cares are needed for these patients.</p>","PeriodicalId":8052,"journal":{"name":"Annals of Clinical Microbiology and Antimicrobials","volume":"24 1","pages":"38"},"PeriodicalIF":4.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12175473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of sublineage diversity on intrinsic susceptibility of Beijing genotype Mycobacterium tuberculosis.","authors":"Haoran Li, Guyue Zhang, Zichun Ma, Haiping Guo, Yuanyuan Shang, Cong Yao, Shanshan Li, Yu Pang, Junhua Pan","doi":"10.1186/s12941-025-00807-6","DOIUrl":"10.1186/s12941-025-00807-6","url":null,"abstract":"<p><p>Tuberculosis (TB) remains a significant global health issue, with drug-resistant TB posing a major challenge. The genetic lineage of Mycobacterium tuberculosis (Mtb) is known to influence various aspects, including drug resistance. Still, the relationship between different lineages and drug resistance levels, especially in the context of the Beijing genotype, requires further exploration. This study aimed to investigate the disparities in drug resistance among diverse lineages of Mtb. We analyzed 193 clinical isolates from drug-resistant TB patients, among them 91.2% were MDR/pre-XDR-TB. Samples were collected from patients at specific hospitals between 2014 and 2020. The isolates were subjected to smear microscopy, sputum culture, minimum inhibitory concentration (MIC) testing, and whole-genome sequencing (WGS). The MIC distributions and resistance levels of drugs like INH, AMK, RIF, EMB, and FQ were analyzed, and the association between lineages and drug resistance was determined using statistical tests. Our results showed significant differences in the MIC distributions and resistance levels of INH and AMK between lineages 2.2 and 2.3. Lineage 2.3.2 was a protective factor for high-level INH resistance, and lineage 2.3 was a protective factor for high-level AMK resistance. The L2.3.6 strain had a high proportion of high-level resistance to INH and AMK. This study provides evidence for the evolution and spread of the modern Beijing genotype of Mtb. It suggests that L2.3.6 will have the potential to become the main sublineage of tuberculosis for the spread of drug-resistant tuberculosis and the necessity of pedigree testing of drug-resistant strains in clinical treatment.</p>","PeriodicalId":8052,"journal":{"name":"Annals of Clinical Microbiology and Antimicrobials","volume":"24 1","pages":"37"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12168258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into the spread of vancomycin- and tigecycline-resistant Enterococcus faecium ST117.","authors":"Marie Brajerova, Otakar Nyc, Pavel Drevinek, Marcela Krutova","doi":"10.1186/s12941-025-00806-7","DOIUrl":"10.1186/s12941-025-00806-7","url":null,"abstract":"<p><strong>Background: </strong>Since the incidence of vancomycin-resistant enterococci (VRE) is increasing and treatment options remain limited, we aimed to investigate the epidemiology of vancomycin- and tigecycline-resistant enterococci in a university hospital using whole genome sequencing (WGS).</p><p><strong>Methods: </strong>Between April and December 2021, 102 VRE isolates were collected from a single tertiary care hospital in the Czech Republic. Forty selected isolates underwent antimicrobial susceptibility testing and WGS (Illumina short reads and long reads with MinION in selected isolates).</p><p><strong>Results: </strong>All Enterococcus faecium isolates were resistant to ampicillin, carrying the PBP5_Met485Ala, PBP5_Glu629Val, and fluoroquinolones carrying the GyrA_Ser83Ile and ParC_Ser80Ile substitutions. The vanA operon was found on pELF2-like plasmids and plasmids carrying rep17 and/or rep18b genes. The novel Tn1546 structural variants were identified in vanA-carrying isolates. The vanB operon was located on the chromosome within a Tn1549 structural variant. Linezolid resistance was detected in one isolate carrying the 23S rDNA_G2576T substitution. Twenty-two isolates were resistant to tigecycline (tet(L), tet(M) and rpsJ_del 155-166 or RpsJ_Lys57Arg). Discrepancies between phenotypic and genotypic resistance profiles were observed for daptomycin (RpoB_Ser491Phe), trimethoprim/sulfamethoxazole (dfrG gene), nitrofurantoin (NmrA_Gln48Lys substitution without the EF0404 and EF0648 genes) and tetracycline (truncated TetM). The two multilocus sequence typing (MLST) schemes identified different numbers of STs: 5 STs, with ST117 as the predominant one (n = 32, 80%), versus 10 STs, with ST138 (27.5%), ST136 (25%), and ST1067 (20%) being the most frequent, respectively. The whole genome MLST revealed clonal clustering (0-7 allele differences) among isolates of the same ST. When comparing ST117 isolates from our study with 2,204 ST117 isolates from 15 countries, only one Czech isolate clustered closely with strains from Germany and the Netherlands, differing by just 16 alleles.</p><p><strong>Conclusions: </strong>The spread of E. faecium isolates ST117 resistant to vancomycin and tigecycline was identified. The discrepancies between resistance genotypes and phenotypes highlight the importance of combining molecular and phenotypic surveillance in antimicrobial resistance monitoring.</p>","PeriodicalId":8052,"journal":{"name":"Annals of Clinical Microbiology and Antimicrobials","volume":"24 1","pages":"36"},"PeriodicalIF":4.6,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}