Annals of botany最新文献

{"title":"How important is important? A commentary on 'Temporal turnover of Ceratobasidiaceae orchid mycorrhizal fungal communities with ontogenetic and phenological development in Prasophyllum (Orchidaceae)'.","authors":"Hans Jacquemyn","doi":"10.1093/aob/mcae142","DOIUrl":"10.1093/aob/mcae142","url":null,"abstract":"","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":"i-ii"},"PeriodicalIF":3.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The smallest angiosperm genomes may be the price for effective traps of bladderworts.","authors":"František Zedek, Jakub Šmerda, Aneta Halasová, Lubomír Adamec, Adam Veleba, Klára Plačková, Petr Bureš","doi":"10.1093/aob/mcae107","DOIUrl":"10.1093/aob/mcae107","url":null,"abstract":"<p><strong>Background: </strong>Species of the carnivorous family Lentibulariaceae exhibit the smallest genomes in flowering plants. We explored the hypothesis that their minute genomes result from the unique mitochondrial cytochrome c oxidase (COX) mutation. The mutation may boost mitochondrial efficiency, which is especially useful for suction-bladder traps of Utricularia, but also increase DNA-damaging reactive oxygen species, leading to genome shrinkage through deletion-biased DNA repair. We aimed to explore the impact of this mutation on genome size, providing insights into genetic mutation roles in plant genome evolution under environmental pressures.</p><p><strong>Methods: </strong>We compiled and measured genome and mean chromosome sizes for 127 and 67 species, respectively, representing all three genera (Genlisea, Pinguicula and Utricularia) of Lentibulariaceae. We also isolated and analysed COX sequences to detect the mutation. Through phylogenetic regressions and Ornstein-Uhlenbeck models of trait evolution, we assessed the impact of the COX mutation on the genome and chromosome sizes across the family.</p><p><strong>Results: </strong>Our findings reveal significant correlations between the COX mutation and smaller genome and chromosome sizes. Specifically, species carrying the ancestral COX sequence exhibited larger genomes and chromosomes than those with the novel mutation. This evidence supports the notion that the COX mutation contributes to genome downsizing, with statistical analyses confirming a directional evolution towards smaller genomes in species harbouring these mutations.</p><p><strong>Conclusions: </strong>Our study confirms that the COX mutation in Lentibulariaceae is associated with genome downsizing, probably driven by increased reactive oxygen species production and subsequent DNA damage requiring deletion-biased repair mechanisms. While boosting mitochondrial energy output, this genetic mutation compromises genome integrity and may potentially affect recombination rates, illustrating a complex trade-off between evolutionary advantages and disadvantages. Our results highlight the intricate processes by which genetic mutations and environmental pressures shape genome size evolution in carnivorous plants.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":"1131-1138"},"PeriodicalIF":3.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11688529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal turnover of Ceratobasidiaceae orchid mycorrhizal fungal communities with ontogenetic and phenological development in Prasophyllum (Orchidaceae).","authors":"Marc Freestone, Noushka Reiter, Nigel D Swarts, Celeste C Linde","doi":"10.1093/aob/mcae089","DOIUrl":"10.1093/aob/mcae089","url":null,"abstract":"<p><strong>Background and aims: </strong>Plant-fungus symbioses may experience temporal turnover during the ontogenetic or phenological development of the host, which can influence the ecological requirements of the host plant. In this study, we investigate temporal turnover of Ceratobasidiaceae orchid mycorrhizal fungal (OMF) communities in Prasophyllum (Orchidaceae), asking whether OMF communities are subject to temporal change attributable to orchid phenology or ontogeny.</p><p><strong>Methods: </strong>Roots of adult Prasophyllum frenchii, Prasophyllum lindleyanum and Prasophyllum sp. aff. validum from Australia were sampled between autumn and spring. Seed was sown in situ as 'baits' to explore the mycorrhizal associations of germinating protocorms, which were compared with OMF in roots of co-occurring adult plants. Culture-dependent and -independent sequencing methods were used to amplify the internal transcribed spacer and mitochondrial large subunit loci, with sequences assigned to operational taxonomic units (OTUs) in phylogenetic analyses. Germination trials were used to determine whether fungal OTUs were mycorrhizal.</p><p><strong>Key results: </strong>A persistent core of OMF was associated with Prasophyllum, with Ceratobasidiaceae OMF dominant in all three species. Phenological turnover occurred in P. lindleyanum and P. sp. aff. validum, but not in P. frenchii, which displayed specificity to a single OTU. Ontogenetic turnover occurred in all species. However, phenological and ontogenetic turnover was typically driven by the presence or absence of infrequently detected OTUs in populations that otherwise displayed specificity to one or two dominant OTUs. Ex situ germination trials showed that 13 of 14 tested OTUs supported seed germination in their host orchid, including eight OTUs that were not found in protocorms in situ.</p><p><strong>Conclusions: </strong>An understanding of OMF turnover can have practical importance for the conservation of threatened orchids and their mycorrhizal partners. However, frameworks for classifying OMF turnover should focus on OTUs important to the life cycle of the host plant, which we suggest are likely to be those that are frequently detected or functionally significant.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":"933-948"},"PeriodicalIF":3.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetics and plasticity of white leaf mark variegation in white clover (Trifolium repens L.).","authors":"Wen-Hsi Kuo, Eimear Cunningham, Emily Guo, Kenneth M Olsen","doi":"10.1093/aob/mcae129","DOIUrl":"10.1093/aob/mcae129","url":null,"abstract":"<p><strong>Background and aims: </strong>Leaf variegation is common in plants and confers diverse adaptive functions. However, its genetic underpinnings remain largely unresolved; this is particularly true for variegation that arises through modified leaf tissue structure that affects light reflection. White clover is naturally polymorphic for structure-based white leaf mark variegation. It therefore provides a useful system in which to examine the genetic basis of this phenotype and to assess potential costs to photosynthetic efficiency resulting from modified leaf structures. In this study, we sought to map the loci controlling the white leaf mark in white clover and to evaluate the relationship between white leaf mark, leaf thickness and photosynthetic efficiency.</p><p><strong>Methods: </strong>We generated a high-density genetic linkage map from an F3 mapping population, using reference genome-based single nucleotide polymorphism markers. White leaf mark was quantified through detailed phenotypic evaluations alongside leaf thickness to test how tissue thickness might affect the variegation phenotype. Mapping of quantitative trait loci was performed to characterize their genetic basis. Photosynthetic efficiency measurements were used to test for physiological trade-offs between variegation and photosynthetic output.</p><p><strong>Key results: </strong>The V locus, a major gene responsible for the white leaf mark polymorphism, was mapped to the distal end of chromosome 5, and several modifier loci were also mapped that contribute additively to the intensity of variegation. The presence and intensity of white leaf mark were associated with greater leaf thickness; however, increased variegation did not affect photosynthetic efficiency detectably.</p><p><strong>Conclusions: </strong>We have successfully mapped the major locus governing the white leaf mark in white clover, along with several modifier loci, revealing a complex basis for this structure-based variegation. The apparent absence of compromised photosynthesis in variegated leaves challenges the notion that variegation creates fitness trade-offs between photosynthetic efficiency and other adaptive functions. This finding suggests that other factors might maintain the white leaf mark polymorphism in white clover.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":"949-958"},"PeriodicalIF":3.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141900747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of the basic helix-loop-helix transcription factor SPATULA and its role in gynoecium development.","authors":"Ana C Rivarola-Sena, Aurélie C Vialette, Amélie Andres-Robin, Pierre Chambrier, Loïc Bideau, Jose M Franco-Zorrilla, Charles P Scutt","doi":"10.1093/aob/mcae140","DOIUrl":"10.1093/aob/mcae140","url":null,"abstract":"<p><strong>Background and aims: </strong>SPATULA (SPT) encodes a basic helix-loop-helix transcription factor in Arabidopsis thaliana that functions in the development of the style, stigma and replum tissues, all of which arise from the carpel margin meristem of the gynoecium. Here we use a comparative approach to investigate the evolutionary history of SPT and identify changes that potentially contributed to its role in gynoecium development.</p><p><strong>Methods: </strong>We investigate SPT's molecular and functional evolution using phylogenetic reconstruction, yeast two-hybrid analyses of protein-protein interactions, microarray-based analyses of protein-DNA interactions, plant transformation assays, RNA in situ hybridization, and in silico analyses of promoter sequences.</p><p><strong>Key results: </strong>We demonstrate the SPT lineage to have arisen at the base of euphyllophytes from a clade of potentially light-regulated transcription factors through gene duplication followed by the loss of an active phytochrome binding (APB) domain. We also clarify the more recent evolutionary history of SPT and its paralogue ALCATRAZ (ALC), which appear to have arisen through a large-scale duplication within Brassicales. We find that SPT orthologues from diverse groups of seed plants share strikingly similar capacities for protein-protein and protein-DNA interactions, and that SPT coding regions from a wide taxonomic range of plants are able to complement loss-of-function spt mutations in transgenic Arabidopsis. However, the expression pattern of SPT appears to have evolved significantly within angiosperms, and we identify structural changes in SPT's promoter region that correlate with the acquisition of high expression levels in tissues arising from the carpel margin meristem in Brassicaceae.</p><p><strong>Conclusions: </strong>We conclude that changes in SPT's expression pattern made a major contribution to the evolution of its developmental role in the gynoecium of Brassicaceae. By contrast, the main biochemical capacities of SPT, as well as many of its immediate transcriptional targets, appear to have been conserved at least since the base of living angiosperms.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":"1037-1054"},"PeriodicalIF":3.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced seed viability in exchange for transgenerational plant protection in an endophyte-symbiotic grass: does the defensive mutualism concept pass the fitness test?","authors":"Benjamin Fuchs, Annelie Damerau, Baoru Yang, Anne Muola","doi":"10.1093/aob/mcae133","DOIUrl":"10.1093/aob/mcae133","url":null,"abstract":"<p><strong>Background and aims: </strong>Epichloë endophytes are vertically transmitted via grass seeds and chemically defend their hosts against herbivory. Endophyte-conferred plant defence via alkaloid biosynthesis might occur independently of costs for host plant growth. However, fitness consequences of endophyte-conferred defence and transgenerational effects on herbivore resistance of progeny plants are rarely studied. The aim of this study was to test whether severe defoliation in mother plants affects their seed production, seed germination rate and the endophyte-conferred resistance of progeny plants.</p><p><strong>Methods: </strong>In a field study, we tested the effects of defoliation and endophyte symbiosis (Epichloë uncinata) on host plant (Festuca pratensis) performance, loline alkaloid concentrations in leaves and seeds, seed biomass and seed germination rates. In a subsequent greenhouse study, we challenged the progeny of the plants from the field study to aphid herbivory and tested whether defoliation of mother plants affects endophyte-conferred resistance against aphids in progeny plants.</p><p><strong>Key results: </strong>Defoliation of the mother plants resulted in a reduction of alkaloid concentrations in leaves and elevated the alkaloid concentrations in seeds when compared with non-defoliated endophyte-symbiotic plants. Viability and germination rate of seeds of defoliated endophyte-symbiotic plants were significantly lower compared with those of non-defoliated endophyte-symbiotic plants and endophyte-free (defoliated and non-defoliated) plants. During 6 weeks of growth, seedlings of defoliated endophyte-symbiotic mother plants had elevated alkaloid concentrations, which was negatively correlated with aphid performance.</p><p><strong>Conclusions: </strong>Endophyte-conferred investment in higher alkaloid levels in seeds, elicited by defoliation, provided protection from herbivores in progenies during the first weeks of plant establishment. Better protection of seeds via high alkaloid concentrations was negatively correlated with seed germination, indicating a trade-off between protection and viability.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":"993-1002"},"PeriodicalIF":3.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141915983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How many species are there? Lineage diversification and hidden speciation in Solanaceae from highland grasslands in southern South America.","authors":"Luana S Soares, Aureliano Bombarely, Loreta B Freitas","doi":"10.1093/aob/mcae144","DOIUrl":"10.1093/aob/mcae144","url":null,"abstract":"<p><strong>Background and aims: </strong>Species delimitation can be challenging when analysing recently diverged species, especially those taxonomically synonymized owing to morphological similarities. We aimed to untangle the relationships between two grassland species, Petunia guarapuavensis and Petunia scheideana, exploring the dynamics of fast divergence and addressing their species delimitation.</p><p><strong>Methods: </strong>We used a low-coverage genome sequencing and population genomic approach to distinguish species and populations between P. guarapuavensis and P. scheideana. Our analysis focused on detecting structuration, hybridization/introgression and phylogenetic patterns. We used demographic models to support species delimitation while exploring potential phylogeographical barriers influencing gene flow.</p><p><strong>Key results: </strong>Our findings indicated differentiation between the two species and revealed another lineage, which was phylogenetically distinct from the others and had no evidence of gene flow with them. The presence of a river acted as a phylogeographical barrier, limiting gene flow and allowing for structuration between closely related lineages. The optimal species delimitation scenario involved secondary contact between well-established lineages.</p><p><strong>Conclusions: </strong>The rapid divergence observed in these Petunia species explains the lack of significant morphological differences, because floral diagnostic traits in species sharing pollinators tend to evolve more slowly. This study highlights the complexity of species delimitation in recently diverged groups and emphasizes the importance of genomic approaches in understanding evolutionary relationships and speciation dynamics.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":"1291-1305"},"PeriodicalIF":3.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11688538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142085942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High metabolic rates drive tiny genomes in plants (and birds): a commentary on 'The smallest angiosperm genomes may be the price for effective traps of bladderworts'.","authors":"Thomas J Givnish","doi":"10.1093/aob/mcae163","DOIUrl":"10.1093/aob/mcae163","url":null,"abstract":"","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":"i-iv"},"PeriodicalIF":3.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11688525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeographical patterns match the floristic subdivisions: the diversification history of a widespread herb in subtropical China.","authors":"Zhixia Sun, Lihua Yang, Hanghui Kong, Ming Kang, Jing Wang","doi":"10.1093/aob/mcae157","DOIUrl":"10.1093/aob/mcae157","url":null,"abstract":"<p><strong>Background and aims: </strong>Subtropical China is dominated by evergreen broad-leaved forests (EBLFs) and is acknowledged as a critical region for its high floristic richness and endemism. Our understanding of the evolutionary mechanisms of such global biodiversity hotspots comes almost exclusively from long-lived tree species. Herbaceous plants represent critical biodiversity components in forests, but the diversification history of understorey herbs in subtropical EBLFs remain poorly understood. Here, we investigated the phylogeographical patterns and demographic history of Oreocharis auricula, a widespread perennial herb endemic to the EBLFs of subtropical China.</p><p><strong>Methods: </strong>Both chloroplast DNA sequences and single-copy nuclear genes were used to investigate the genetic variation among 657 individuals from 68 populations. Evidence from molecular dating, demographic history construction and species distribution modelling was also combined to infer the phylogeography and evolutionary history of O. auricula.</p><p><strong>Key results: </strong>Strong phylogeographical signals have been congruently observed using nuclear and plastid DNA markers, with the diversification patterns generally consistent with the recognized floristic subdivisions of subtropical China. Notably, we revealed an important phylogeographical barrier along the Nanling mountain range, which is also around a climatic transition at 24-26°N latitude in subtropical China, separating the south monsoon subtropical EBLFs from the mid-subtropical EBLFs. Demographic expansion and significant niche divergence were detected among the extant lineages, which may have diverged during the early Pleistocene.</p><p><strong>Conclusions: </strong>The inherent characteristics of understorey herbs with limited dispersal and short generation time intensify the genetic divergence response of O. auricula to abiotic forces, contributing to the profound phylogeographical imprints of mountains and climate in such herbaceous flora. To further substantiate the generality of the identified patterns, it is paramount to extend phylogeographical investigations to other understorey herbaceous taxa in subtropical China. These results have expanded our understanding of the diversification processes of subtropical forests in China.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":"1263-1276"},"PeriodicalIF":3.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11688537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Confusing boundaries of the Labrador tea species: dispersal history explains the lack of clear species structure.","authors":"D R Iunusova, M A Polezhaeva, E A Marchuk","doi":"10.1093/aob/mcae130","DOIUrl":"10.1093/aob/mcae130","url":null,"abstract":"<p><strong>Background and aims: </strong>The Labrador teas (genus Rhododendron, subsection Ledum) are a complex of species widely distributed in the Northern Hemisphere. They occupy cold-resistant plant communities from highlands to forest understorey and wetland habitats almost circumboreally and they are especially abundant in Northeast Asia and northern North America, yet there are no clear species boundaries in this group. The genetic structure of species of subsect. Ledum from Eurasia and North America as well as the dispersal history of the group require clarification.</p><p><strong>Methods: </strong>The phylogeny and biogeography of subsect. Ledum of the genus Rhododendron were assessed using phylogenetic trees constructed based on the analysis of variation in chloroplast petB-petD, trnV-ndhC, trnH-psbA, K2R-K707, atpB oligo2 - rbcL oligo5 and nuclear (ITS1) markers of four Eurasian and one American species (65 populations, 408 individuals). The data were evaluated with maximum parsimony and Bayesian analysis. Molecular dating and reconstruction of ancestral areas were performed.</p><p><strong>Key results: </strong>Dense sampling revealed widespread presence of shared haplotypes and ribotypes among Ledum populations and species. Two American, three mixed and one Eurasian lineage diversified during the Neogene climate cooling and then rapidly dispersed during the Pleistocene. The ability to accumulate high genetic diversity and to preserve it across distribution ranges and generations prevented Ledum from lineage sorting. As a result, a species complex with a reserve of genetic variability appeared.</p><p><strong>Conclusions: </strong>Although no clear phylogenetic inference can be obtained at present, the plastid genealogy is consistent with the nuclear genealogy and demonstrates the processes involved in speciation in the Ledum species complex.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":"959-974"},"PeriodicalIF":3.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141900788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}