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Methods in cell science : an official journal of the Society for In Vitro Biology最新文献

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Chromosome painting in farm, pet and wild animal species. 农场、宠物和野生动物的染色体绘制。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_6
B. Chowdhary, T. Raudsepp
{"title":"Chromosome painting in farm, pet and wild animal species.","authors":"B. Chowdhary, T. Raudsepp","doi":"10.1007/978-94-010-0330-8_6","DOIUrl":"https://doi.org/10.1007/978-94-010-0330-8_6","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"117 1","pages":"37-55"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79319281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 47
Human chromosomal banding by in situ hybridization of isochores. 用同工染色体原位杂交法测定人类染色体带。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_2
S. Saccone, G. Bernardi
{"title":"Human chromosomal banding by in situ hybridization of isochores.","authors":"S. Saccone, G. Bernardi","doi":"10.1007/978-94-010-0330-8_2","DOIUrl":"https://doi.org/10.1007/978-94-010-0330-8_2","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"26 1","pages":"7-15"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83273371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
GISH technology in plant genome research. 植物基因组研究中的GISH技术。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_9
S. Raina, V. Rani
{"title":"GISH technology in plant genome research.","authors":"S. Raina, V. Rani","doi":"10.1007/978-94-010-0330-8_9","DOIUrl":"https://doi.org/10.1007/978-94-010-0330-8_9","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"85 1","pages":"83-104"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81238162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 66
Induction of reduced endothelial permeability to horseradish peroxidase by factor(s) of human astrocytes and bladder carcinoma cells: detection in multi-well plate culture. 人星形胶质细胞和膀胱癌细胞因子诱导根过氧化物酶内皮通透性降低:多孔平板培养检测。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1023/a:1016399822589
H Fukushima, M Iwasaki, O Yosie, H Igarashi
{"title":"Induction of reduced endothelial permeability to horseradish peroxidase by factor(s) of human astrocytes and bladder carcinoma cells: detection in multi-well plate culture.","authors":"H Fukushima,&nbsp;M Iwasaki,&nbsp;O Yosie,&nbsp;H Igarashi","doi":"10.1023/a:1016399822589","DOIUrl":"https://doi.org/10.1023/a:1016399822589","url":null,"abstract":"<p><p>Endothelial permeability to horseradish peroxidase (HRP) was assayed in multi-well plate culture. Confluent cobblestone-monolayers of endothelial cells were incubated with HRP, and the amounts of HRP that permeated the monolayer and reached the bottom substrate were estimated by extraction of HRP with deoxycholate and following addition of chromogenic substrate of HRP into the extracts. Using this in situ assay method, we detected the reduced permeability to HRP in aortic and brain microvascular endothelial cells after 4-5 days culture with conditioned media of human astrocytes or bladder carcinoma cells. Preliminary characterization of the active factors(s) released from these cells was performed. This method will be useful for monitoring impermeable endothelial cell monolayers and identifying the active factor(s).</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"23 4","pages":"211-9"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1016399822589","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22159251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
An improved method for culture of epidermal keratinocytes from newborn mouse skin. 一种培养新生小鼠表皮角质形成细胞的改进方法。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1023/a:1016385109922
L Häkkinen, L Koivisto, H Larjava
{"title":"An improved method for culture of epidermal keratinocytes from newborn mouse skin.","authors":"L Häkkinen,&nbsp;L Koivisto,&nbsp;H Larjava","doi":"10.1023/a:1016385109922","DOIUrl":"https://doi.org/10.1023/a:1016385109922","url":null,"abstract":"<p><p>Reproducible isolation and long term culture of epidermal keratinocytes from transgenic mouse lines is critically needed but most techniques have been unsuccessful. In this report we describe in detail a simplified method to isolate putative keratinocyte stem cells from newborn mouse skin and to maintain them for long term in culture. The cell cultures were established by enzymatically separating keratinocytes from newborn mouse skin. For selecting the putative keratinocyte stem cells for culture, the cells are allowed to attach for 10 minutes on a composite matrix made of type I collagen and fibronectin. Unattached cells were discarded and the attached cells were cultured in a defined culture medium containing low Ca2+ concentration, 9% FBS, conditioned medium from newborn mouse skin fibroblasts, and EGF. For subculturing, the cells were seeded on tissue culture plastic. The isolated cells showed the typical basal keratinocyte morphology and expressed the epithelial cell specific integrin alpha v beta 6. The expression level of alpha v beta 6 integrin was comparable to human skin keratinocytes. The keratinocytes were also able to differentiate to form an epidermis in an organotypic culture model. By using the described protocol, the keratinocytes from frozen stocks have been subcultured up to 26 times without change in cell viability, proliferation rate or morphology.</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"23 4","pages":"189-96"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1016385109922","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22159248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 60
Fluorescent in situ hybridization in plant polytene chromosomes. 植物多丝染色体的荧光原位杂交。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_13
M. Guerra
{"title":"Fluorescent in situ hybridization in plant polytene chromosomes.","authors":"M. Guerra","doi":"10.1007/978-94-010-0330-8_13","DOIUrl":"https://doi.org/10.1007/978-94-010-0330-8_13","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"27 1","pages":"133-8"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89864655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Chromosome painting as a tool for rice genetics and breeding. 染色体绘画作为水稻遗传育种的工具。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_12
R. Shishido, N. Ohmido, K. Fukui
{"title":"Chromosome painting as a tool for rice genetics and breeding.","authors":"R. Shishido, N. Ohmido, K. Fukui","doi":"10.1007/978-94-010-0330-8_12","DOIUrl":"https://doi.org/10.1007/978-94-010-0330-8_12","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"1 1","pages":"125-32"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90289776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Modification of cell perifusion for extended study of hormone release in the rat pituitary. 大鼠垂体激素释放的细胞灌注修饰研究。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1023/a:1016339932088
S Vella, J Gussick, M Woller, D Waechter-Brulla
{"title":"Modification of cell perifusion for extended study of hormone release in the rat pituitary.","authors":"S Vella,&nbsp;J Gussick,&nbsp;M Woller,&nbsp;D Waechter-Brulla","doi":"10.1023/a:1016339932088","DOIUrl":"https://doi.org/10.1023/a:1016339932088","url":null,"abstract":"<p><p>We use a flow-through cell perifusion system allowing continuous sampling of Luteinizing Hormone (LH) release from excised pituitary tissue. With this system, we are able to sample from viable tissue in primary culture for three days as opposed to a more typical of primary culture of 6-12 hours. Rat pituitary tissue was perifused and challenged with a physiological dose of LHRH. Samples were collected for 3.5 hours on each of 3 consecutive days in a refrigerated fraction collector and assayed for LH concentration by radioimmunoassay. In these experiments we tested two types of media, each with or without fetal calf serum supplementation, for their ability to support pituitary tissue for extended lengths of perifusion. In addition, we broadened the use of aseptic technique, sterile media and equipment to limit microbial contamination. Our results indicate that perifusion of pituitary tissue can be done successfully for 3 or more days at a modest cost, greatly increasing the amount of information that can be collected from each piece of tissue harvested.</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"23 4","pages":"197-204"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1016339932088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22159249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Microdissection and chromosome painting of plant B chromosomes. 植物B染色体的显微解剖与染色。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_11
A. Houen, B. Field, V. Saunders
{"title":"Microdissection and chromosome painting of plant B chromosomes.","authors":"A. Houen, B. Field, V. Saunders","doi":"10.1007/978-94-010-0330-8_11","DOIUrl":"https://doi.org/10.1007/978-94-010-0330-8_11","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"54 1","pages":"115-24"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86602296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Molecular cytogenetics of introgressive hybridization in plants. 植物渐渗杂交的分子细胞遗传学。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_14
K. Anamthawat-Jónsson
{"title":"Molecular cytogenetics of introgressive hybridization in plants.","authors":"K. Anamthawat-Jónsson","doi":"10.1007/978-94-010-0330-8_14","DOIUrl":"https://doi.org/10.1007/978-94-010-0330-8_14","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"132 1","pages":"139-48"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88481763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 37
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