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Methods in cell science : an official journal of the Society for In Vitro Biology最新文献

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Preparation of dissociated zebrafish spinal neuron cultures. 分离斑马鱼脊髓神经元培养物的制备。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1023/a:1016349232389
S S Andersen
{"title":"Preparation of dissociated zebrafish spinal neuron cultures.","authors":"S S Andersen","doi":"10.1023/a:1016349232389","DOIUrl":"https://doi.org/10.1023/a:1016349232389","url":null,"abstract":"<p><p>The method described here explains a simple protocol for how to prepare dissociated Zebrafish spinal neuron cultures. The neurons grow fast in a simple culture medium and at room temperature. Considering the advantages afforded by the optical transparency of the Zebrafish embryo combined with the powerful molecular perturbation techniques available, this technique has potential to further advance molecular analysis of axon growth and guidance.</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"23 4","pages":"205-9"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1016349232389","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22159250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative karyotype using bidirectional chromosome painting: how and why? 使用双向染色体绘制的比较核型:如何以及为什么?
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_17
M. Guilly, L. Dano, P. de Chamisso, P. Fouchet, B. Dutrillaux, S. Chevillard
{"title":"Comparative karyotype using bidirectional chromosome painting: how and why?","authors":"M. Guilly, L. Dano, P. de Chamisso, P. Fouchet, B. Dutrillaux, S. Chevillard","doi":"10.1007/978-94-010-0330-8_17","DOIUrl":"https://doi.org/10.1007/978-94-010-0330-8_17","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"36 1","pages":"163-70"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82184757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Identification and analysis of sex chromosomes by comparative genomic hybridization (CGH). 性染色体比较基因组杂交(CGH)鉴定与分析。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_16
W. Traut, U. Eickhof, J. C. Schorch
{"title":"Identification and analysis of sex chromosomes by comparative genomic hybridization (CGH).","authors":"W. Traut, U. Eickhof, J. C. Schorch","doi":"10.1007/978-94-010-0330-8_16","DOIUrl":"https://doi.org/10.1007/978-94-010-0330-8_16","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"1 1","pages":"155-61"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79907995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 29
The use of FISH in chromosomal localization of transgenes in rice. FISH在水稻转基因染色体定位中的应用。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_10
J. Dong, P. Kharb, M. Cervera, T. Hall
{"title":"The use of FISH in chromosomal localization of transgenes in rice.","authors":"J. Dong, P. Kharb, M. Cervera, T. Hall","doi":"10.1007/978-94-010-0330-8_10","DOIUrl":"https://doi.org/10.1007/978-94-010-0330-8_10","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"38 1","pages":"105-13"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76354908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Isolation of vascular smooth muscle cells from a single murine aorta. 从单个小鼠主动脉中分离出血管平滑肌细胞。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1023/a:1016357510143
J L Ray, R Leach, J M Herbert, M Benson
{"title":"Isolation of vascular smooth muscle cells from a single murine aorta.","authors":"J L Ray, R Leach, J M Herbert, M Benson","doi":"10.1023/a:1016357510143","DOIUrl":"10.1023/a:1016357510143","url":null,"abstract":"<p><p>The vascular smooth muscle cell plays a significant role in many important cardiovascular disorders, and smooth muscle biology is therefore important to cardiovascular research. The mouse is critical to basic cardiovascular research, largely because techniques for genetic manipulation are more fully developed in the mouse than in any other mammalian species. We describe here a technique for isolating smooth muscle cells from a single mouse aorta. This technique is particularly useful when material is limiting, as is frequently the case when genetically modified animals are being characterized.</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"23 4","pages":"185-8"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1016357510143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22159852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 260
Telomere length measurement by Q-FISH. 用Q-FISH测定端粒长度。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_3
P. Slijepcevic
{"title":"Telomere length measurement by Q-FISH.","authors":"P. Slijepcevic","doi":"10.1007/978-94-010-0330-8_3","DOIUrl":"https://doi.org/10.1007/978-94-010-0330-8_3","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"44 1","pages":"17-22"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84564996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 66
Comparing qualitative and quantitative spectroscopic techniques for the detection of the effect of direct iron loading of mammalian cell cultures. 比较定性和定量光谱技术对哺乳动物细胞培养物直接铁负载效果的检测。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1023/a:1016313221027
H N Traore, D Meyer
{"title":"Comparing qualitative and quantitative spectroscopic techniques for the detection of the effect of direct iron loading of mammalian cell cultures.","authors":"H N Traore,&nbsp;D Meyer","doi":"10.1023/a:1016313221027","DOIUrl":"https://doi.org/10.1023/a:1016313221027","url":null,"abstract":"<p><p>Iron overload augments diseases of the liver and microorganism infection as well as deregulates the immune system. In vitro analysis of the effects of iron loading and its chelation involves determining the amount of iron constituting overload, which metal sources and cell lines to use and reliable assay methods. The uptake of 500 microM FeSO4 or FeEDTA by CEMss, U937 or leukocytes was confirmed by inductively coupled plasma-atomic emission spectroscopy (ICP-AES). Excess iron increased CEMss viability (assessed by MTT, XTT, Trypan- and Alamar Blue) by an average of 18% (P = 0.034). Flow cytometry indicated dye-viable cells to be undergoing apotosis/necrosis while still confirming an increase (9%, P < 0.001) in excess iron-induced viability. The iron chelator desferioxamine (DFO) when added in addition to Fe, reversed the effects of excess iron (and vice versa) and had detrimental effects when used on its own (33% inhibition of viability as measured by dyes and 10.85%; P = 0.0427 assessed by flow cytometry). The 4 dyes demonstrated different levels of sensitivity in detecting the influence of iron or DFO but gave a related, qualitative picture while flow cytometry and ICP-AES data was more quantitative.</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"23 4","pages":"175-84"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1016313221027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22159851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
High resolution FISH to delineate contiguous and small DNA sequences. 高分辨率FISH描绘连续和小的DNA序列。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_15
U. C. Lavania
{"title":"High resolution FISH to delineate contiguous and small DNA sequences.","authors":"U. C. Lavania","doi":"10.1007/978-94-010-0330-8_15","DOIUrl":"https://doi.org/10.1007/978-94-010-0330-8_15","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"142 1","pages":"149-54"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78780460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Relationship of telomere sequence and constitutive heterochromatin in the human and apes as detected by PRINS. 人类和类人猿端粒序列与组成异染色质的关系。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1023/a:1013181213276
H. Hirai
{"title":"Relationship of telomere sequence and constitutive heterochromatin in the human and apes as detected by PRINS.","authors":"H. Hirai","doi":"10.1023/a:1013181213276","DOIUrl":"https://doi.org/10.1023/a:1013181213276","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"100 1","pages":"29-35"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74827955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Chromosome painting in plants. 植物的染色体绘画。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1023/A:1013137415093
Ingo Schubert, P. Fransz, Jörg Fuchs, J. de Jong
{"title":"Chromosome painting in plants.","authors":"Ingo Schubert, P. Fransz, Jörg Fuchs, J. de Jong","doi":"10.1023/A:1013137415093","DOIUrl":"https://doi.org/10.1023/A:1013137415093","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"5 1","pages":"57-69"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83622951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 142
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