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Methods in cell science : an official journal of the Society for In Vitro Biology最新文献

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Localisation of DNA sequences on plant chromosomes using PRINS and C-PRINS. 利用PRINS和C-PRINS定位植物染色体DNA序列。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_8
M. Kubaláková, J. Vrána, J. Číhalíková, M. Lysak, J. Doležel
{"title":"Localisation of DNA sequences on plant chromosomes using PRINS and C-PRINS.","authors":"M. Kubaláková, J. Vrána, J. Číhalíková, M. Lysak, J. Doležel","doi":"10.1007/978-94-010-0330-8_8","DOIUrl":"https://doi.org/10.1007/978-94-010-0330-8_8","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"4 1","pages":"71-82"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84545220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 31
Culture methods for turtle lymphocytes. 龟淋巴细胞培养方法。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2000-12-01 DOI: 10.1023/A:1017559301372
B. Ulsh, J. Congdon, T. Hinton, F. W. Whicker, J. Bedford
{"title":"Culture methods for turtle lymphocytes.","authors":"B. Ulsh, J. Congdon, T. Hinton, F. W. Whicker, J. Bedford","doi":"10.1023/A:1017559301372","DOIUrl":"https://doi.org/10.1023/A:1017559301372","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"84 1","pages":"285-97"},"PeriodicalIF":0.0,"publicationDate":"2000-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90928925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
Methods for animal satellite cell culture under a variety of conditions. 动物卫星细胞在各种条件下的培养方法。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2000-03-01 DOI: 10.1023/a:1009830114804
N M Burton, J Vierck, L Krabbenhoft, K Bryne, M V Dodson
{"title":"Methods for animal satellite cell culture under a variety of conditions.","authors":"N M Burton,&nbsp;J Vierck,&nbsp;L Krabbenhoft,&nbsp;K Bryne,&nbsp;M V Dodson","doi":"10.1023/a:1009830114804","DOIUrl":"https://doi.org/10.1023/a:1009830114804","url":null,"abstract":"<p><p>Primary and clonal culture systems have been devised and refined for animal-derived satellite cells. Satellite cell (SC) culture development includes efficient cell isolation techniques, establishment of effective plating and growth conditions, formulation of media requirements and thorough evaluation of experimental limitations. As the field of muscle cell culture has expanded, the number of animal species from which satellite cells have been isolated has increased. The focus of this paper is to compare and contrast SC culture conditions presently used by a variety of researchers and to introduce a new source of SC from wapiti (elk).</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"22 1","pages":"51-61"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1009830114804","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21505568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 48
List of contributors 贡献者名单
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2000-03-01 DOI: 10.1023/A:1017247208927
{"title":"List of contributors","authors":"","doi":"10.1023/A:1017247208927","DOIUrl":"https://doi.org/10.1023/A:1017247208927","url":null,"abstract":"","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"22 1","pages":"25"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/A:1017247208927","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21505563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ten commandments for preventing contamination of primary cell cultures. 防止原代细胞培养物污染的十诫。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2000-03-01 DOI: 10.1023/a:1009826012986
J L Vierck, K Byrne, P S Mir, M V Dodson
{"title":"Ten commandments for preventing contamination of primary cell cultures.","authors":"J L Vierck,&nbsp;K Byrne,&nbsp;P S Mir,&nbsp;M V Dodson","doi":"10.1023/a:1009826012986","DOIUrl":"https://doi.org/10.1023/a:1009826012986","url":null,"abstract":"<p><p>Procedures for preventing contamination in primary cell cultures must be carefully defined and strictly followed in order to obtain healthy cells. Protocols have been developed and refined in our laboratory for establishing primary cultures of muscle and fat stem cells without contamination from a variety of animals. Contamination of cell cultures is not only frustrating, but is also very expensive both in time and loss of materials. Through the consistent use of proper aseptic techniques, most instances of contamination may be avoided. We suggest that the basic principles detailed here will find wide applicability in the culturing of primary cells without contamination from many different types of animals and tissues.</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"22 1","pages":"33-41"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1009826012986","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21505566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Inhibition of prostaglandin synthesis reduces cyclic AMP levels and inhibits chondrogenesis in cultured chick limb mesenchyme. 抑制前列腺素合成可降低环AMP水平并抑制培养鸡肢体间质软骨形成。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2000-03-01 DOI: 10.1023/a:1009824106368
D M Biddulph, M M Dozier, A A Capehart
{"title":"Inhibition of prostaglandin synthesis reduces cyclic AMP levels and inhibits chondrogenesis in cultured chick limb mesenchyme.","authors":"D M Biddulph,&nbsp;M M Dozier,&nbsp;A A Capehart","doi":"10.1023/a:1009824106368","DOIUrl":"https://doi.org/10.1023/a:1009824106368","url":null,"abstract":"<p><p>The present study investigated effects of inhibiting the synthesis of prostaglandins (PGs) on cyclic AMP concentrations and chondrogenesis in cultured chick limb mesenchyme. Indomethacin produced concentration-dependent inhibition of both PGE(2) synthesis and chondrogenesis over a concentration range of 50--200 microM. Half maximal inhibition of PGE(2) was achieved with 50 microM concentrations of the drug which also produced visibly reduced amounts of cartilage matrix in cell cultures as evaluated by Alcian green staining on day 6 of culture. The inhibitory effects of indomethacin on chondrogenesis were largely reversed by addition of 1 mM dibutyryl cAMP, indicating that cells could still respond to cyclic AMP stimulation. Endogenous levels of cyclic AMP, which increased by 6 fold during the six days of culture in control cells, did not increase significantly from dissociated cells at the time of plating (day 0) in indomethacin- treated cultures. The results indicate that inhibition of the prechondrogenic rise in PGE(2) concentrations in limb mesenchyme prevents the increase in cyclic AMP levels which occur during this same period resulting in inhibition of chondrogenesis. The data provide further support for the hypothesis that PGE(2), through its effects on the adenylate cyclase-cAMP system, plays an important role in the differentiation of cartilage.</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"22 1","pages":"9-16"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1009824106368","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21505664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Generation of useful cell culture data. 生成有用的细胞培养数据。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2000-03-01 DOI: 10.1023/a:1009886125765
M V Dodson, W I Schaeffer
{"title":"Generation of useful cell culture data.","authors":"M V Dodson,&nbsp;W I Schaeffer","doi":"10.1023/a:1009886125765","DOIUrl":"https://doi.org/10.1023/a:1009886125765","url":null,"abstract":"<p><p>As the need for viable and interpretable cell culture systems increases, it is not sufficient to simply be successful at growing cells in vitro. Rather, vigilance is required to obtain repeatable data from these systems, especially if mechanistic or developmental experimental designs are attempted. We suggest that all aspects of basic cell culture are as important as growing cells. We offer the papers of this issue to help the cell scientist scrutinize and identify problems in many of these important areas, including obtaining tissue, eliminating microbial contamination, formulating a defined medium, isolating specific cell types from tissue and then using them for in vitro studies, cloning cells, selecting and developing methods for cell culture analyses, and recognizing abnormal cell culture activity.</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"22 1","pages":"27-8"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1009886125765","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21505564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Thoughts on the source of tissue on subsequent cell culture success. 组织来源对后续细胞培养成功的思考。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2000-03-01 DOI: 10.1023/a:1009876618921
S E Reedy, D M Powell, N M Williams, M V Dodson, B P Fitzgerald
{"title":"Thoughts on the source of tissue on subsequent cell culture success.","authors":"S E Reedy,&nbsp;D M Powell,&nbsp;N M Williams,&nbsp;M V Dodson,&nbsp;B P Fitzgerald","doi":"10.1023/a:1009876618921","DOIUrl":"https://doi.org/10.1023/a:1009876618921","url":null,"abstract":"<p><p>This paper describes attempts to initiate equine adipocyte cultures from necropsy cases with varying intervals from time of death to isolation and culture. Equine adipocytes were isolated from 21 necropsy cases, regardless of the interval from time after death to establishment in primary ceiling cultures. However, while all cultures produced adipocytes, only 2 attempts to produce long-term equine adipocyte cultures from the subcutaneous rump fat depots were successful and not contaminated. Findings from these experiments indicate that it is possible to collect and culture equine adipocytes from necropsy cases with varying intervals of time of death to culturing provided that the issue of contamination is addressed. Viable cells were produced from tissue with an interval of 38.5 hours as well as 45 minutes. This result encourages the continuation of research using equine necropsy cases as a source of adipose tissue.</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"22 1","pages":"29-32"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1009876618921","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21505565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Traditional and emerging methods for analyzing cell activity in cell culture. 分析细胞培养中细胞活性的传统方法和新兴方法。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2000-03-01 DOI: 10.1023/a:1009839501174
N T Stewart, K M Byrne, H L Hosick, J L Vierck, M V Dodson
{"title":"Traditional and emerging methods for analyzing cell activity in cell culture.","authors":"N T Stewart,&nbsp;K M Byrne,&nbsp;H L Hosick,&nbsp;J L Vierck,&nbsp;M V Dodson","doi":"10.1023/a:1009839501174","DOIUrl":"https://doi.org/10.1023/a:1009839501174","url":null,"abstract":"<p><p>The selection of appropriate techniques to assay for markers of cell activity is important for obtaining optimal results in cell culture-based research. This paper is intended as a guide to many of the assays currently available and new techniques that have been recently introduced in the literature. This paper addresses both manual assay techniques, including the use of hemocytometers, phase contrast microscopy, cell staining, and the immunofluorescent antibody assay (IFA), and automated assays for cell activity, including stained optical density, proliferating cell nuclear antigen, creatine kinase assay, DNA quantification, electronic cell counting, flow cytometry, magnetic cell sorting, image analysis, chemiluminescence, radioisotope labeling, precursor incorporation, in-situ hybridization/ligand binding, and enzyme-linked immuno-culture assay (ELICA). Advantages/disadvantages and applicability of these assays to different areas of cell culture research are discussed, and guidelines for selecting an appropriate assay are suggested.</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"22 1","pages":"67-78"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1009839501174","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21505570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
Establishment of a phagocytic cell line from Bombina orientalis. 东方bombomina orientalis吞噬细胞系的建立。
Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2000-03-01 DOI: 10.1023/a:1009879808494
S C Park, S H Lee, S S Han
{"title":"Establishment of a phagocytic cell line from Bombina orientalis.","authors":"S C Park,&nbsp;S H Lee,&nbsp;S S Han","doi":"10.1023/a:1009879808494","DOIUrl":"https://doi.org/10.1023/a:1009879808494","url":null,"abstract":"<p><p>Continuous serum-free culture of Bok-2 cells was generated from primary culture of the tail bud stage embryos of the toad, Bombina orientalis. Bok-2 cells can be maintained in modified L-15 serum-free medium prepared by mixing L-15 medium, lactalbumin enzymatic hydrolysate, sucrose and sodium bicarbonate. Bok-2 cells have an ameboid behavior and morphology. When Bok-2 and viable Candida albicans were co-cultured, Bok-2 cells showed an immune response characterized by chemotaxis, phagocytosis and partial clearing activity of Candida cells and colonies. And Bok-2 cells also showed phagocytosis of latex beads without serum treatment and displayed numerous pseudopodia, membrane evagination for phagocytosis and phagosome activity. On the basis of these results, Bok-2 cells were identified as professional phagocytes.</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"22 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1009879808494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21505663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
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