Methods in cell science : an official journal of the Society for In Vitro Biology最新文献_第7页

Analysis of heterogeneous red cell populations by flow cytometry. 流式细胞术分析异质红细胞群。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01

M Nelson

引用次数: 0

Cell array coupled with laser scanning cytometry allows easy analysis of changes in cyclin expression during the cell cycle. An application of cell array system. 细胞阵列与激光扫描细胞术相结合，可以轻松分析细胞周期蛋白表达的变化。小区阵列系统的一个应用。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1023/a:1024181512088

Tomoko Furuya, Morihito Takita, Shin-ichi Tsunoda, Shigeto Kawauchi, Takashi Hirano, Atsunori Oga, Kohsuke Sasaki

{"title":"Cell array coupled with laser scanning cytometry allows easy analysis of changes in cyclin expression during the cell cycle. An application of cell array system.","authors":"Tomoko Furuya, Morihito Takita, Shin-ichi Tsunoda, Shigeto Kawauchi, Takashi Hirano, Atsunori Oga, Kohsuke Sasaki","doi":"10.1023/a:1024181512088","DOIUrl":"https://doi.org/10.1023/a:1024181512088","url":null,"abstract":"To assess the potential of cell array technology, a cell array slide with 50 spots was designed specifically for automated analysis of changes in expression of cyclins A and B1 during the cell cycle at the cellular level. Cells harvested every 1 hour from 0 through 23 hours after synchronization by mitotic selection were spotted in duplicate on a glass slide. Each slide contained 48 spots representing 24 different cell cycle phases, and the remaining two spots were peripheral blood lymphocytes, which were included as negative control. This cell array provided temporal and spatial information related to changes in cyclin expression during the cell cycle in a single experiment. The present study indicates that expression analysis by cell array is novel approach for cell cycle studies. Furthermore, sophisticated multiplexed cell array technology has great potential for analyses of expression of specific genes during diverse cellular events at the cellular level.","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"24 1-3","pages":"41-7"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1024181512088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22444201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measurement of protein synthesis and degradation in C2C2) myoblasts using extracts of muscle from hormone treated bovine. 用激素处理牛肌肉提取物测定C2C2成肌细胞蛋白质合成和降解。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1023/a:1024498316958

J L Montgomery, W M Harper, M F Miller, K J Morrow, J R Blanton

{"title":"Measurement of protein synthesis and degradation in C2C2) myoblasts using extracts of muscle from hormone treated bovine.","authors":"J L Montgomery, W M Harper, M F Miller, K J Morrow, J R Blanton","doi":"10.1023/a:1024498316958","DOIUrl":"https://doi.org/10.1023/a:1024498316958","url":null,"abstract":"A detailed methodology is described for determination of treatment effects on muscle cell protein synthesis and muscle cell protein degradation in a cell culture system. C(2)C(22) mouse myoblasts were treated with growth media containing muscle extracts from bovine treated with different pharmaceutical agents. Radiolabeled amino acids were added to the growth media to determine treatment effects on protein synthesis and protein degradation. Percent protein synthesis was calculated by measuring amino acid uptake as a percentage over internal control. Percent protein degradation was measured using a pulse chase technique. These procedures will allow researchers to determine treatment effects on overall protein synthesis and degradation in vitro in a relatively short amount of time without excessive costs. A second benefit is that animals do not have to be fed radiolabeled feedstuffs. These procedures are not intended to elucidate the mechanisms behind pharmaceutical enhancement of muscle cell protein synthesis or protein degradation.","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"24 4","pages":"123-9"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1024498316958","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22468986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Analysis of intracellular cytokines using flowcytometry. 流式细胞术分析细胞内细胞因子。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1007/978-94-017-0623-0_5

S. Arora

引用次数: 23

Nitric oxide mediated modulation of free radical generation response in the rat polymorphonuclear leukocytes: a flowcytometric study. 一氧化氮介导的大鼠多形核白细胞自由基生成反应的调节:一项流式细胞术研究。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1007/978-94-017-0623-0_11

M. Dikshit, Prashant Sharma

引用次数: 11

Cellular and serological markers of disease activity in Indian patients with HIV/AIDS. 印度艾滋病毒/艾滋病患者疾病活动的细胞和血清学标志物。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01

S Sehgal, U Datta, S Mujtaba, A Sood, V K Vinayak

{"title":"Cellular and serological markers of disease activity in Indian patients with HIV/AIDS.","authors":"S Sehgal, U Datta, S Mujtaba, A Sood, V K Vinayak","doi":"","DOIUrl":"","url":null,"abstract":"There has been an exponential rise of HIV positive patients as observed at the surveillance center of Nehru Hospital. Most patients are poor and cannot afford repeated viral load assays. Therefore, there is a need to identify cost effective and reliable surrogate markers of disease activity. In the present study absolute number of CD4 cells, beta2 micro-globulin, circulating nucleosomes were studied in 30 patients of AIDS, 30 seropositives and 30 healthy controls. In addition viral load, P-24 assay, and TNFR-II assays were done in seropositive and AIDS patients. The mean CD4 cells in patients with AIDS were 69.66 +/- 68.25 mm3 while in seropositives values was 370 +/- 201.29 mm3. The mean CD4 cells in healthy controls were however 690 +/- 198 mm3. The differences in all the groups were highly significant (p<0.001). The mean CD4 values in Indians are significantly lower than reported from the west. The lower number of CD4 cells in healthy population is interpreted to be due to immune activation. The CD8 cell number in controls was 650 +/- 207 mm3 this figure is also higher than that observed in the west. P-24 assay failed to delineate between seropositives and patients with AIDS. Although, beta2 microglobulin levels were significantly higher in AIDS than in seropositives and higher in seropositives than in controls yet with the best possible cut off, it had a sensitivity of only 70% in delineating the two conditions. The correlation between CD4 cells and viral load was more significant when the CD4 cells were below 200 mm3. Five out of 30 patients with a CD4 of 300-600 mm3 had a viral load of over 1 x 10(5) cop/ml. The difference in TNF R-II levels between seropositives and AIDS was however more impressive. With a cut off of 550 pg/ml it had a sensitivity of 95% in delineating HIV from AIDS. It is concluded that a combination of absolute number of CD4 cells and TNF R-II assay along with clinical evaluation may be used to monitor therapy in resource poor countries where frequent viral load assay is unaffordable.","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"24 1-3","pages":"107-14"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22444715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of heterogeneous red cell populations by flow cytometry. 流式细胞术分析异质红细胞群。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1007/978-94-017-0623-0_3

M. Nelson

引用次数: 4

Effects of temperature on the susceptibility of insect cells to infection by baculoviruses. 温度对昆虫细胞对杆状病毒感染敏感性的影响。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1023/a:1016394421408

D E Lynn

{"title":"Effects of temperature on the susceptibility of insect cells to infection by baculoviruses.","authors":"D E Lynn","doi":"10.1023/a:1016394421408","DOIUrl":"https://doi.org/10.1023/a:1016394421408","url":null,"abstract":"Three insect cell lines were tested for susceptibility to baculovirus infection by use of a typical endpoint assay procedure. Cell lines from Spodoptera frugiperda (IPLB-Sf21AE), Lymantria dispar (IPLB-LdEIta), and Heliothis virescens (IPLB-HvE6s) in 96-well tissue culture plates were each infected with dilutions of extra cellular virus suspensions of the Autographa californica nucleopolyhedrovirus (AcMNPV). In addition, the L. dispar and H. virescens cells were also infected with L. dispar nucleopolyhedrovirus, and Helicoverpa zea nucleopolyhedrovirus, respectively. Each cell/virus combination was incubated at three temperatures: 22, 27 and 32 degrees C and wells were scored for positive infection (presence of occlusion bodies in cell nuclei) at 2 to 4 day intervals for up to 4 weeks. The resulting data were analyzed by the Spearman-Kärber method, providing virus titers for each combination of virus, cell line, and temperature. The results were categorized by accuracy (assuming the highest titer achieved was the most accurate) and by rapidity of maximum titer. AcMNPV reached the highest titer in each line at 22 degrees C although equivalent titers were reached with both AcMNPV and HzSNPV in the HvE6a line at all three temperatures. This line actually reported about 100-fold less AcMNPV than the other two lines with the same virus sample. Alternatively, the Sf21AE and LdEIta lines reached 10-fold higher titers at the lowest temperature as compared with the higher temperatures, although also at a slower rate.","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"23 4","pages":"221-5"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1016394421408","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22159252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Immunolocalization of HP1 proteins in metaphasic mammalian chromosomes. 哺乳动物中期染色体中HP1蛋白的免疫定位。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_18

E. Minc, Yves Allory, J. Courvalin, Brigitte Buendia

引用次数: 46

Refined characterisation of chromosome aberrations in tumours by multicolour banding and electronic mapping resources. 利用多色带和电子图谱资源对肿瘤染色体畸变进行精细表征。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2001-01-01 DOI: 10.1007/978-94-010-0330-8_4

D. Gisselsson

引用次数: 23