Annals of Biomedical Engineering最新文献

{"title":"Potential Use of ChatGPT for the Treatment of Infectious Diseases in Vulnerable Populations.","authors":"Heqing Tao, Ligang Liu, Jiayu Cui, Kunkun Wang, Liang Peng, Milap C Nahata","doi":"10.1007/s10439-024-03600-2","DOIUrl":"https://doi.org/10.1007/s10439-024-03600-2","url":null,"abstract":"<p><strong>Background: </strong>ChatGPT could be a useful tool in the infectious disease field. However, the application of ChatGPT for the treatment of infectious diseases in vulnerable population has not been determined.</p><p><strong>Methods: </strong>We designed questions about antibiotic use, including the choice of antibiotics, dose, and treatment duration for prevalent infectious disease in vulnerable populations. Each query was posed to ChatGPT-4, and the answers were independently evaluated by two authors. When there were significant differences in the final scores between the two authors, they discussed the case and answers to obtain results.</p><p><strong>Results: </strong>Our analysis revealed that 38.1% of responses were comprehensive and correct, with 11.9% containing errors for medication use for patients during pregnancy. For the antibiotic allergy-related questions, 36.1% of responses were comprehensive and correct, and 18.1% contained errors. For older adults, 27.5% of responses were comprehensive and correct, while 25% contained errors. The error rate in patients with kidney disease was 79.2%. For children, 43.8% of answers contained errors.</p><p><strong>Conclusion: </strong>ChatGPT produced high rates of inaccurate information for treating infectious diseases in special population. Thus, recommendations generated by ChatGPT should be used with caution and checked by healthcare professionals to ensure accuracy and comprehensiveness prior to use.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141915976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calibration of Aseptic Loosening Simulation for Coatings Osteoinductive Effect.","authors":"Sofia Baroni, Sara Oliviero, Antonino Amedeo La Mattina, Melania Maglio, Lucia Martini, Milena Fini, Marco Viceconti","doi":"10.1007/s10439-024-03588-9","DOIUrl":"https://doi.org/10.1007/s10439-024-03588-9","url":null,"abstract":"<p><p>The risk of aseptic loosening in cementless hip stems can be reduced by improving osseointegration with osteoinductive coatings favoring long-term implant stability. Osseointegration is usually evaluated in vivo studies, which, however, do not reproduce the mechanically driven adaptation process. This study aims to develop an in silico model to predict implant osseointegration and the effect of induced micromotion on long-term stability, including a calibration of the material osteoinductivity with conventional in vivo studies. A Finite Element model of the tibia implanted with pins was generated, exploiting bone-to-implant contact measures of cylindrical titanium alloys implanted in rabbits' tibiae. The evolution of the contact status between bone and implant was modeled using a finite state machine, which updated the contact state at each iteration based on relative micromotion, shear and tensile stresses, and bone-to-implant distance. The model was calibrated with in vivo data by identifying the maximum bridgeable gap. Afterward, a push-out test was simulated to predict the axial load that caused the macroscopic mobilization of the pin. The bone-implant bridgeable gap ranged between 50 μm and 80 μm. Predicted push-out strength ranged from 19 N to 21 N (5.4 MPa-3.4 MPa) depending on final bone-to-implant contact. Push-out strength agrees with experimental measurements from a previous animal study (4 ± 1 MPa), carried out using the same implant material, coated, or uncoated. This method can partially replace in vivo studies and predict the long-term stability of cementless hip stems.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Image-Based Computational Fluid Dynamics to Compare Two Repair Techniques for Mitral Valve Prolapse.","authors":"Lorenzo Bennati, Giovanni Puppini, Vincenzo Giambruno, Giovanni Battista Luciani, Christian Vergara","doi":"10.1007/s10439-024-03597-8","DOIUrl":"https://doi.org/10.1007/s10439-024-03597-8","url":null,"abstract":"<p><p>OBJECTIVE : The treatment of mitral valve prolapse involves two distinct repair techniques: chordal replacement (Neochordae technique) and leaflet resection (Resection technique). However, there is still a debate in the literature about which is the optimal one. In this context, we performed an image-based computational fluid dynamic study to evaluate blood dynamics in the two surgical techniques. METHODS : We considered a healthy subject (H) and two patients (N and R) who underwent surgery for prolapse of the posterior leaflet and were operated with the Neochordae and Resection technique, respectively. Computational Fluid Dynamics (CFD) was employed with prescribed motion of the entire left heart coming from cine-MRI images, with a Large Eddy Simulation model to describe the transition to turbulence and a resistive method for managing valve dynamics. We created three different virtual scenarios where the operated mitral valves were inserted in the same left heart geometry of the healthy subject to study the differences attributed only to the two techniques. RESULTS : We compared the three scenarios by quantitatively analyzing ventricular velocity patterns and pressures, transition to turbulence, and the ventricle ability to prevent thrombi formation. From these results, we found that the operative techniques affected the ventricular blood dynamics in different ways, with variations attributed to the reduced mobility of the Resection posterior leaflet. Specifically, the Resection technique resulted in turbulent forces, related with the risk of hemolysis formation, up to 640 Pa, while the other two scenarios exhibited a maximum of 240 Pa. Moreover, in correspondence of the ventricular apex, the Resection technique reduced the areas with low velocity to 15%, whereas the healthy case and the Neochordae case maintained these areas at 30 and 48%, respectively. Our findings suggest that the Neochordae technique developed a more physiological flow with respect to the Resection technique. CONCLUSION: Resection technique gives rise to a different direction of the mitral jet during diastole increasing the ability to washout the ventricular apex preventing from thrombi formation, but at the same time it promotes turbulence formation that is associated with ventricular effort and risk of hemolysis.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Simple Analytical Model to Facilitate Preoperative Surgical Planning in Valve-Sparing Aortic Root Replacement.","authors":"Dominic P Recco, Shannen B Kizilski, Wen Zhang, Nicholas E Kneier, Patrick D Earley, David M Hoganson, Christopher W Baird, Peter E Hammer","doi":"10.1007/s10439-024-03593-y","DOIUrl":"https://doi.org/10.1007/s10439-024-03593-y","url":null,"abstract":"<p><strong>Purpose: </strong>Valve-sparing root replacement (VSRR) is attractive for aortic root dilation as it preserves the native aortic valve (AoV). Low effective height (eH) after reconstruction is a risk factor for repair failure and reoperation. We developed and validated a quantitative AoV repair strategy to reliably restore normal valve proportions to promote long-term function.</p><p><strong>Methods: </strong>Normal AoV proportions were used to derive geometric relationships for sinotubular junction diameter (D_STJ), free edge length (FEL), free edge angle, and commissure height. These relationships informed two models for predicting eH following VSRR: (1) assuming valve symmetry and (2) accounting for valve asymmetry. Porcine heart (n = 6) ex vivo validation was performed under 4 VSRR scenarios: \"Ideal\" (tube graft size targeting FEL/D_STJ = 1.28), \"Oversized\" (one graft size larger than Ideal), \"Undersized\" (two sizes smaller), and \"Undersized + Plicated\" (FEL/D_STJ = 1.28 restored with leaflet plication).</p><p><strong>Results: </strong>Our analytical models predicted eH using preoperative measurements and estimated reconstructed dimensions. The Oversized graft exhibited similar eH to Ideal but higher regurgitation in the ex vivo model, whereas the Undersized graft demonstrated lower eH and regurgitation. Plication in the Undersized graft restored valve function (regurgitation & eH) similar to Ideal in the ex vivo model and above Ideal in the analytical models. Both analytical models predicted ex vivo eH well except in the Oversized and Undersized + Plicated conditions.</p><p><strong>Conclusion: </strong>Utilizing measurements from preoperative imaging and simple mathematical models, patient-specific operative plans for VSRR can be created by estimating valve dimensions necessary to achieve favorable valve features post-repair. Clinical application of this approach promises to improve consistency in achieving optimal long-term dimensions and durability.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141896584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Energy and Pressure in the Sinus with Different Blood Pressures after Bioprosthetic Aortic Valve Replacement.","authors":"Brennan Vogl, Agata Sularz, Sunyoung Ahn, Rajat Gadhave, Scott Lilly, Vinod Thourani, Brian Lindman, Mohamad Alkhouli, Hoda Hatoum","doi":"10.1007/s10439-024-03587-w","DOIUrl":"https://doi.org/10.1007/s10439-024-03587-w","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the effect of changing systolic and diastolic blood pressures (SBP and DBP, respectively) on sinus flow and valvular and epicardial coronary flow dynamics after TAVR and SAVR.</p><p><strong>Methods: </strong>SAPIEN 3 and Magna valves were deployed in an idealized aortic root model as part of a pulse duplicating left heart flow loop simulator. Different combinations of SBP and DBP were applied to the test setup and the resulting change in total coronary flow from baseline (120/60 mmHg), effective orifice area (EOA), and left ventricular (LV) workload, with each combination, was assessed. In addition, particle image velocimetry was used to assess the Laplacian of pressure ( <math> <mrow> <msup><mrow><mi>∇</mi></mrow> <mn>2</mn></msup> <mtext>P</mtext></mrow> </math> ) in the sinus, coronary and main flow velocities, the energy dissipation rate (EDR) in the sinus and the LV workload.</p><p><strong>Results: </strong>This study shows that under an elevated SBP, there is an increase in the total coronary flow, EOA, LV workload, peak velocities downstream of the valve, <math> <mrow> <msup><mrow><mi>∇</mi></mrow> <mn>2</mn></msup> <mtext>P</mtext></mrow> </math> , and EDR. With an elevated DBP, there was an increase in the total coronary flow and <math> <mrow> <msup><mrow><mi>∇</mi></mrow> <mn>2</mn></msup> <mtext>P</mtext></mrow> </math> . However, EOA and LV workload decreased with an increase in DBP, and EDR increased with a decrease in DBP.</p><p><strong>Conclusions: </strong>Blood pressure alters the hemodynamics in the sinus and downstream flow following aortic valve replacement, potentially influencing outcomes in some patients.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141892687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclic Mechanism Affects Lumbar Spine Creep Response.","authors":"Elizabeth D Dimbath, Concetta Morino, Shea Middleton, Jason Kait, Maria Ortiz-Paparoni, Theodore A Slotkin, Jason F Luck, Cameron R 'Dale' Bass","doi":"10.1007/s10439-024-03595-w","DOIUrl":"https://doi.org/10.1007/s10439-024-03595-w","url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to explore how cyclic loading influences creep response in the lumbar spine under combined flexion-compression loading.</p><p><strong>Methods: </strong>Ten porcine functional spinal units (FSUs) were mechanically tested in cyclic or static combined flexion-compression loading. Creep response between loading regimes was compared using strain-time histories and linear regression. High-resolution computed tomography (µCT) visualized damage to FSUs. Statistical methods, ANCOVA and ANOVA, assessed differences in behavior between loading regimes.</p><p><strong>Results: </strong>Cyclic and static loading regimes exhibited distinct creep response patterns and biphasic response. ANCOVA and ANOVA analyses revealed significant differences in slopes of creep behavior in both linear phases. Cyclic tests consistently showed endplate fractures in µCT imaging.</p><p><strong>Conclusion: </strong>The study reveals statistically significant differences in creep response between cyclic and static loading regimes in porcine lumbar spinal units under combined flexion-compression loading. The observed biphasic behavior suggests distinct phases of tissue response, indicating potential shifts in load transfer mechanisms. Endplate fractures in cyclic tests suggest increased injury risk compared to static loading. These findings underscore the importance of considering loading conditions in computational models and designing preventive measures for occupations involving repetitive spinal loading.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational-Aided Approach for the Optimization of Microfluidic-Based Nanoparticles Manufacturing Process.","authors":"Marco Bellotti, Enrica Chiesa, Bice Conti, Ida Genta, Michele Conti, Ferdinando Auricchio, Alessandro Caimi","doi":"10.1007/s10439-024-03590-1","DOIUrl":"10.1007/s10439-024-03590-1","url":null,"abstract":"<p><p>In the last few years, the microfluidic production of nanoparticles (NPs) is becoming a promising alternative to conventional industrial approaches (e.g., nanoprecipitation, salting out, and emulsification-diffusion) thanks to the production efficiency, low variability, and high controllability of the production parameters. Nevertheless, the development of new formulations and the switching of the production process toward microfluidic platforms requires expensive and time-consuming number of experiments for the tuning of the formulation to obtain NPs with specific morphological and functional characteristics. In this work, we developed a computational fluid dynamic pipeline, validated through an ad hoc experimental strategy, to reproduce the mixing between the solvent and anti-solvent (i.e., acetonitrile and TRIS-HCl, respectively). Moreover, beyond the classical variables able to describe the mixing performances of the microfluidic chip, novel variables were described in order to assess the region of the NPs formation and the changing of the amplitude of the precipitation region according to different hydraulic conditions. The numerical approach proved to be able to capture a progressive reduction of the nanoprecipitation region due to an increment of the flow rate ratio; in parallel, through the experimental production, a progressive increment of the NPs size heterogeneity was observed with the same fluid dynamic conditions. Hence, the preliminary comparison between numerical and experimental evidence proved the effectiveness of the computational strategy to optimize the NPs manufacturing process.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting Knee Joint Contact Force Peaks During Gait Using a Video Camera or Wearable Sensors.","authors":"Jere Lavikainen, Lauri Stenroth, Paavo Vartiainen, Tine Alkjær, Pasi A Karjalainen, Marius Henriksen, Rami K Korhonen, Mimmi Liukkonen, Mika E Mononen","doi":"10.1007/s10439-024-03594-x","DOIUrl":"https://doi.org/10.1007/s10439-024-03594-x","url":null,"abstract":"<p><strong>Purpose: </strong>Estimating loading of the knee joint may be helpful in managing degenerative joint diseases. Contemporary methods to estimate loading involve calculating knee joint contact forces using musculoskeletal modeling and simulation from motion capture (MOCAP) data, which must be collected in a specialized environment and analyzed by a trained expert. To make the estimation of knee joint loading more accessible, simple input predictors should be used for predicting knee joint loading using artificial neural networks.</p><p><strong>Methods: </strong>We trained feedforward artificial neural networks (ANNs) to predict knee joint loading peaks from the mass, height, age, sex, walking speed, and knee flexion angle (KFA) of subjects using their existing MOCAP data. We also collected an independent MOCAP dataset while recording walking with a video camera (VC) and inertial measurement units (IMUs). We quantified the prediction accuracy of the ANNs using walking speed and KFA estimates from (1) MOCAP data, (2) VC data, and (3) IMU data separately (i.e., we quantified three sets of prediction accuracy metrics).</p><p><strong>Results: </strong>Using portable modalities, we achieved prediction accuracies between 0.13 and 0.37 root mean square error normalized to the mean of the musculoskeletal analysis-based reference values. The correlation between the predicted and reference loading peaks varied between 0.65 and 0.91. This was comparable to the prediction accuracies obtained when obtaining predictors from motion capture data.</p><p><strong>Discussion: </strong>The prediction results show that both VCs and IMUs can be used to estimate predictors that can be used in estimating knee joint loading outside the motion laboratory. Future studies should investigate the usability of the methods in an out-of-laboratory setting.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-field Head Acceleration Exposure Measurements Using Instrumented Mouthguards: Multi-stage Screening to Optimize Data Quality","authors":"Adam C. Clansey,&nbsp;Daniel Bondi,&nbsp;Rebecca Kenny,&nbsp;David Luke,&nbsp;Zaryan Masood,&nbsp;Yuan Gao,&nbsp;Marko Elez,&nbsp;Songbai Ji,&nbsp;Alexander Rauscher,&nbsp;Paul van Donkelaar,&nbsp;Lyndia C. Wu","doi":"10.1007/s10439-024-03592-z","DOIUrl":"10.1007/s10439-024-03592-z","url":null,"abstract":"<div><p>Instrumented mouthguards (iMGs) are widely applied to measure head acceleration event (HAE) exposure in sports. Despite laboratory validation, on-field factors including potential sensor skull-decoupling and spurious recordings limit data accuracy. Video analysis can provide complementary information to verify sensor data but lacks quantitative kinematics reference information and suffers from subjectivity. The purpose of this study was to develop a rigorous multi-stage screening procedure, combining iMG and video as independent measurements, aimed at improving the quality of on-field HAE exposure measurements. We deployed iMGs and gathered video recordings in a complete university men’s ice hockey varsity season. We developed a four-stage process that involves independent video and sensor data collection (Stage I), general screening (Stage II), cross verification (Stage III), and coupling verification (Stage IV). Stage I yielded 24,596 iMG acceleration events (AEs) and 17,098 potential video HAEs from all games. Approximately 2.5% of iMG AEs were categorized as cross-verified and coupled iMG HAEs after Stage IV, and less than 1/5 of confirmed or probable video HAEs were cross-verified with iMG data during stage III. From Stage I to IV, we observed lower peak kinematics (median peak linear acceleration from 36.0 to 10.9 g; median peak angular acceleration from 3922 to 942 rad/s²) and reduced high-frequency signals, indicative of potential reduction in kinematic noise. Our study proposes a rigorous process for on-field data screening and provides quantitative evidence of data quality improvements using this process. Ensuring data quality is critical in further investigation of potential brain injury risk using HAE exposure data.</p></div>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endothelial Cell Transcription Modulation in Cerebral Aneurysms After Endovascular Flow Diversion.","authors":"Guilherme Barros, Emma Federico, Patrick Fillingham, Pritha Chanana, Naoki Kaneko, Ying Zheng, Louis J Kim, Michael R Levitt","doi":"10.1007/s10439-024-03591-0","DOIUrl":"10.1007/s10439-024-03591-0","url":null,"abstract":"<p><strong>Purpose: </strong>Flow diverting stents (FDS) are used to treat cerebral aneurysms, by promoting thrombosis and occlusion of the aneurysm sac. However, retreatment is required in some cases, and the biologic basis behind treatment outcome is not known. The goal of this study was to understand how changes in hemodynamic flow after FDS placement affect aneurysmal endothelial cell (EC) activity.</p><p><strong>Methods: </strong>Three-dimensional models of patient-specific aneurysms were created to quantify the EC response to FDS placement. Computational fluid dynamic simulations were used to determine the hemodynamic impact of FDS. Two identical models were created for each patient; into one a FDS was inserted. Each model was then populated with human carotid ECs and subjected to patient-specific pulsatile flow for 24 h. ECs were isolated from aneurysm dome from each model and bulk RNA sequencing was performed.</p><p><strong>Results: </strong>Paired untreated and treated models were created for four patients. Aneurysm dome EC analysis revealed 366 (2.6%) significant gene changes between the untreated and FDS conditions, out of 13909 total expressed genes. Gene set enrichment analysis of the untreated models demonstrated enriched gene ontology terms related to cell adhesion, growth/tensile activity, cytoskeletal organization, and calcium ion binding. In the FDS models, enriched terms were related to cellular proliferation, ribosomal activity, RNA splicing, and protein folding.</p><p><strong>Conclusion: </strong>Treatment of cerebral aneurysms with FDS induces significant EC gene transcription changes related to aneurysm hemodynamics in patient-specific in vitro 3D-printed models subjected to pulsatile flow. Further investigation is needed into the relationship between transcriptional change and treatment outcome.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141878243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}