Annual review of biochemistry最新文献_第9页

Quantifying Target Occupancy of Small Molecules Within Living Cells. 活细胞内小分子目标占用的定量分析。

IF 16.6 1区生物学

Annual review of biochemistry Pub Date : 2020-06-20 Epub Date: 2020-03-24 DOI: 10.1146/annurev-biochem-011420-092302

M B Robers, R Friedman-Ohana, K V M Huber, L Kilpatrick, J D Vasta, B-T Berger, C Chaudhry, S Hill, S Müller, S Knapp, K V Wood

{"title":"Quantifying Target Occupancy of Small Molecules Within Living Cells.","authors":"M B Robers, R Friedman-Ohana, K V M Huber, L Kilpatrick, J D Vasta, B-T Berger, C Chaudhry, S Hill, S Müller, S Knapp, K V Wood","doi":"10.1146/annurev-biochem-011420-092302","DOIUrl":"https://doi.org/10.1146/annurev-biochem-011420-092302","url":null,"abstract":"The binding affinity and kinetics of target engagement are fundamental to establishing structure-activity relationships (SARs) for prospective therapeutic agents. Enhancing these binding parameters for operative targets, while minimizing binding to off-target sites, can translate to improved drug efficacy and a widened therapeutic window. Compound activity is typically assessed through modulation of an observed phenotype in cultured cells. Quantifying the corresponding binding properties under common cellular conditions can provide more meaningful interpretation of the cellular SAR analysis. Consequently, methods for assessing drug binding in living cells have advanced and are now integral to medicinal chemistry workflows. In this review, we survey key technological advancements that support quantitative assessments of target occupancy in cultured cells, emphasizing generalizable methodologies able to deliver analytical precision that heretofore required reductionist biochemical approaches.","PeriodicalId":7980,"journal":{"name":"Annual review of biochemistry","volume":"89 ","pages":"557-581"},"PeriodicalIF":16.6,"publicationDate":"2020-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1146/annurev-biochem-011420-092302","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37768803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

How Does the Ribosome Fold the Proteome? 核糖体如何折叠蛋白质组?

IF 16.6 1区生物学

Annual review of biochemistry Pub Date : 2020-06-20 DOI: 10.1146/annurev-biochem-062917-012226

Anaïs M E Cassaignau, Lisa D Cabrita, John Christodoulou

引用次数: 36

Molecular Mechanisms of Natural Rubber Biosynthesis. 天然橡胶生物合成的分子机制。

IF 16.6 1区生物学

Annual review of biochemistry Pub Date : 2020-06-20 Epub Date: 2020-03-30 DOI: 10.1146/annurev-biochem-013118-111107

Satoshi Yamashita, Seiji Takahashi

{"title":"Molecular Mechanisms of Natural Rubber Biosynthesis.","authors":"Satoshi Yamashita, Seiji Takahashi","doi":"10.1146/annurev-biochem-013118-111107","DOIUrl":"https://doi.org/10.1146/annurev-biochem-013118-111107","url":null,"abstract":"Natural rubber (NR), principally comprising cis-1,4-polyisoprene, is an industrially important natural hydrocarbon polymer because of its unique physical properties, which render it suitable for manufacturing items such as tires. Presently, industrial NR production depends solely on latex obtained from the Pará rubber tree, Hevea brasiliensis. In latex, NR is enclosed in rubber particles, which are specialized organelles comprising a hydrophobic NR core surrounded by a lipid monolayer and membrane-bound proteins. The similarity of the basic carbon skeleton structure between NR and dolichols and polyprenols, which are found in most organisms, suggests that the NR biosynthetic pathway is related to the polyisoprenoid biosynthetic pathway and that rubber transferase, which is the key enzyme in NR biosynthesis, belongs to the cis-prenyltransferase family. Here, we review recent progress in the elucidation of molecular mechanisms underlying NR biosynthesis through the identification of the enzymes that are responsible for the formation of the NR backbone structure.","PeriodicalId":7980,"journal":{"name":"Annual review of biochemistry","volume":"89 ","pages":"821-851"},"PeriodicalIF":16.6,"publicationDate":"2020-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1146/annurev-biochem-013118-111107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37784516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47

Christopher Dobson, 1949-2019: Mentor, Friend, Scientist Extraordinaire. 克里斯托弗·多布森，1949-2019:导师、朋友、非凡的科学家。

IF 16.6 1区生物学

Annual review of biochemistry Pub Date : 2020-06-20 Epub Date: 2020-04-28 DOI: 10.1146/annurev-biochem-011520-105226

Carol V Robinson

引用次数: 2

Single-Molecule Studies of Protein Folding with Optical Tweezers. 用光学镊子对蛋白质折叠进行单分子研究。

IF 16.6 1区生物学

Annual review of biochemistry Pub Date : 2020-06-20 DOI: 10.1146/annurev-biochem-013118-111442

Carlos Bustamante, Lisa Alexander, Kevin Maciuba, Christian M Kaiser

引用次数: 0

Mechanisms of Mitochondrial Iron-Sulfur Protein Biogenesis. 线粒体铁硫蛋白生物生成机制。

IF 16.6 1区生物学

Annual review of biochemistry Pub Date : 2020-06-20 Epub Date: 2020-01-14 DOI: 10.1146/annurev-biochem-013118-111540

Roland Lill, Sven-A Freibert

{"title":"Mechanisms of Mitochondrial Iron-Sulfur Protein Biogenesis.","authors":"Roland Lill, Sven-A Freibert","doi":"10.1146/annurev-biochem-013118-111540","DOIUrl":"10.1146/annurev-biochem-013118-111540","url":null,"abstract":"Mitochondria are essential in most eukaryotes and are involved in numerous biological functions including ATP production, cofactor biosyntheses, apoptosis, lipid synthesis, and steroid metabolism. Work over the past two decades has uncovered the biogenesis of cellular iron-sulfur (Fe/S) proteins as the essential and minimal function of mitochondria. This process is catalyzed by the bacteria-derived iron-sulfur cluster assembly (ISC) machinery and has been dissected into three major steps: de novo synthesis of a [2Fe-2S] cluster on a scaffold protein; Hsp70 chaperone-mediated trafficking of the cluster and insertion into [2Fe-2S] target apoproteins; and catalytic conversion of the [2Fe-2S] into a [4Fe-4S] cluster and subsequent insertion into recipient apoproteins. ISC components of the first two steps are also required for biogenesis of numerous essential cytosolic and nuclear Fe/S proteins, explaining the essentiality of mitochondria. This review summarizes the molecular mechanisms underlying the ISC protein-mediated maturation of mitochondrial Fe/S proteins and the importance for human disease.","PeriodicalId":7980,"journal":{"name":"Annual review of biochemistry","volume":"89 ","pages":"471-499"},"PeriodicalIF":16.6,"publicationDate":"2020-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1146/annurev-biochem-013118-111540","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37541295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 170

Mucins and the Microbiome. 粘液和微生物组。

IF 16.6 1区生物学

Annual review of biochemistry Pub Date : 2020-06-20 Epub Date: 2020-04-03 DOI: 10.1146/annurev-biochem-011520-105053

Gunnar C Hansson

{"title":"Mucins and the Microbiome.","authors":"Gunnar C Hansson","doi":"10.1146/annurev-biochem-011520-105053","DOIUrl":"10.1146/annurev-biochem-011520-105053","url":null,"abstract":"Generating the barriers that protect our inner surfaces from bacteria and other challenges requires large glycoproteins called mucins. These come in two types, gel-forming and transmembrane, all characterized by large, highly O-glycosylated mucin domains that are diversely decorated by Golgi glycosyltransferases to become extended rodlike structures. The general functions of mucins on internal epithelial surfaces are to wash away microorganisms and, even more importantly, to build protective barriers. The latter function is most evident in the large intestine, where the inner mucus layer separates the numerous commensal bacteria from the epithelial cells. The host's conversion of MUC2 to the outer mucus layer allows bacteria to degrade the mucin glycans and recover the energy content that is then shared with the host. The molecular nature of the mucins is complex, and how they construct the extracellular complex glycocalyx and mucus is poorly understood and a future biochemical challenge. Expected final online publication date for the Annual Review of Biochemistry, Volume 89 is June 22, 2020. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.","PeriodicalId":7980,"journal":{"name":"Annual review of biochemistry","volume":"89 ","pages":"769-793"},"PeriodicalIF":16.6,"publicationDate":"2020-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1146/annurev-biochem-011520-105053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37797839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 140

Chemical Biology Framework to Illuminate Proteostasis. 阐明蛋白质静止的化学生物学框架。

IF 16.6 1区生物学

Annual review of biochemistry Pub Date : 2020-06-20 Epub Date: 2020-02-25 DOI: 10.1146/annurev-biochem-013118-111552

Rebecca M Sebastian, Matthew D Shoulders

引用次数: 20

Double the Fun, Double the Trouble: Paralogs and Homologs Functioning in the Endoplasmic Reticulum. 乐趣加倍，麻烦加倍:内质网中的类似物和同源物功能。

IF 16.6 1区生物学

Annual review of biochemistry Pub Date : 2020-06-20 DOI: 10.1146/annurev-biochem-011520-104831

Emma J Fenech, Shifra Ben-Dor, Maya Schuldiner

{"title":"Double the Fun, Double the Trouble: Paralogs and Homologs Functioning in the Endoplasmic Reticulum.","authors":"Emma J Fenech, Shifra Ben-Dor, Maya Schuldiner","doi":"10.1146/annurev-biochem-011520-104831","DOIUrl":"https://doi.org/10.1146/annurev-biochem-011520-104831","url":null,"abstract":"The evolution of eukaryotic genomes has been propelled by a series of gene duplication events, leading to an expansion in new functions and pathways. While duplicate genes may retain some functional redundancy, it is clear that to survive selection they cannot simply serve as a backup but rather must acquire distinct functions required for cellular processes to work accurately and efficiently. Understanding these differences and characterizing gene-specific functions is complex. Here we explore different gene pairs and families within the context of the endoplasmic reticulum (ER), the main cellular hub of lipid biosynthesis and the entry site for the secretory pathway. Focusing on each of the ER functions, we highlight specificities of related proteins and the capabilities conferred to cells through their conservation. More generally, these examples suggest why related genes have been maintained by evolutionary forces and provide a conceptual framework to experimentally determine why they have survived selection.","PeriodicalId":7980,"journal":{"name":"Annual review of biochemistry","volume":"89 ","pages":"637-666"},"PeriodicalIF":16.6,"publicationDate":"2020-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1146/annurev-biochem-011520-104831","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38071306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Transcription in Living Cells: Molecular Mechanisms of Bursting. 活细胞转录:爆发的分子机制。

IF 16.6 1区生物学

Annual review of biochemistry Pub Date : 2020-06-20 Epub Date: 2020-03-24 DOI: 10.1146/annurev-biochem-011520-105250

Joseph Rodriguez, Daniel R Larson

引用次数: 112