Annals of Applied Biology最新文献

{"title":"Integration of transcriptional responses in cold contrasting sorghum genotypes under low temperatures","authors":"Pedro Fernando Vera Hernández,&nbsp;Vladimir Flores Benavides,&nbsp;Marcelino Martínez Núñez,&nbsp;Silvia Luna Suárez,&nbsp;Flor de Fátima Rosas Cárdenas","doi":"10.1111/aab.12938","DOIUrl":"10.1111/aab.12938","url":null,"abstract":"<p>Sorghum is a crop that is susceptible to low temperatures. However, due to the extreme global temperatures, new genotypes have been bred to adapt better to cold climates. Therefore, to better understand the metabolic and molecular differences that may contribute to the improved cold tolerance of these genotypes, it is essential to investigate whether specific molecules play a key role in determining the degree of sorghum tolerance. Furthermore, identifying genes associated with cold tolerance may assist in the development of more robust and productive sorghum cultivars. Here, we analysed the changes in selected elements of the antioxidant metabolism, genes, and miRNAs in two sorghum varieties with different degrees of tolerance to cold stress at different times of exposure to cold stress. The high expression of <i>SbCBF6</i> in response to cold treatment in early time in the tolerant variety suggests its involvement in the cold response, thereby facilitating a better adaptation of the plant to cold conditions. MiR398 and miR394 showed contrasting behaviour in both varieties in low temperatures, resulting in interesting miRNAs in the degree of sorghum tolerance. MiR319, a marker for the selection of cold-tolerant cultivars, presents a decrease, suggesting that the better capacity to repress the expression of several members of TCP family of transcription factor genes might be involved in better tolerance to cold stress. This study shows the changes of molecules of interest in response to cold stress in sorghum, marking the difference between cold-susceptible and cold-tolerant sorghum plants, which can contribute to selecting tolerant genotypes, allowing new strategies to enhance crop productivity and food security, in regions where low temperatures significantly constrain crop growth and yield.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"185 3","pages":"410-428"},"PeriodicalIF":2.2,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142214091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frond and mesophyll traits related to photosynthetic capacity and water-use efficiency in ferns with different life-forms ex situ","authors":"Larissa A. Ivanova,&nbsp;Alyona S. Tretyakova,&nbsp;Evgeniy Savitsky,&nbsp;Polina K. Yudina,&nbsp;Leonid A. Ivanov","doi":"10.1111/aab.12934","DOIUrl":"10.1111/aab.12934","url":null,"abstract":"<p>Leaf traits are known as indicative of species functional properties in angiosperms. Fern fronds are little studied in this concern, especially ex situ. We studied leaf mesophyll structure, pigments and gas exchange in four fern species grown in a glasshouse—<i>Sphaeropteris cooperi</i> (terrestrial tree fern), <i>Phlebodium aureum</i> (semi-epiphytic herbaceous), <i>Asplenium australasicum</i> (epiphytic shrubby) and <i>Platycerium bifurcatum</i> (epiphytic herbaceous). Saturated assimilation rate (<i>A</i>_sat) decreased and intrinsic water use efficiency (iWUE) increased from a terrestrial tree fern to epiphytic ferns. <i>A</i>_sat positively correlated with chloroplast number (<i>N</i>_chl/<i>A</i>) and their surface area per leaf area (<i>A</i>_chl/<i>A</i>). iWUE negatively related to <i>N</i>_chl/<i>A</i> and chlorophyll/carotenoid ratio. Most differences between species were found in the mesophyll thickness (MT) and mesophyll cell volume (<i>V</i>_cell) with the smallest values in <i>S. cooperi</i> and the largest ones in <i>P. bifurcatum</i>. We found that photosynthetic limitations in ferns were related to the chloroplast photosynthetic activity rather than to <i>N</i>_chl/<i>A</i> or pigment content which did not differ from most angiosperms. Epiphytic ferns showed larger values of <i>V</i>_cell per cell and per chloroplast compared to angiosperms and tree ferns. We concluded that an increase in MT and <i>V</i>_cell in the studied ferns was not associated with photosynthetic performance, but was related to volumetric cytoplasm–chloroplast ratio meaningful to light absorption and the water-storage function of fern fronds.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"185 3","pages":"396-409"},"PeriodicalIF":2.2,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141924360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of three pheromone lures on trapping the palm weevils Rhynchophorus palmarum and Dynamis borassi (Coleoptera: Curculionidae)","authors":"Mario S. Pinilla-Gallego,&nbsp;Jenny M. Santos-Holguín,&nbsp;Julián Roberto Mejía-Salazar,&nbsp;Vanessa Moncayo,&nbsp;Félix Alberto Guzmán","doi":"10.1111/aab.12931","DOIUrl":"10.1111/aab.12931","url":null,"abstract":"<p>In the Americas, the palm weevils <i>Rhynchophorus palmarum</i> and <i>Dynamis borassi</i> are two of the most economically important palm pests, attacking crops including oil palms, coconut palms, Canary Islands date palms and peach palms. Mass trapping with aggregation pheromone-baited traps has been used for several decades as the main way to monitor and manage palm weevil populations worldwide. Given that several species of palm weevils have the same main aggregation pheromone and that one species can be attracted to the aggregation pheromones of other species, it is important to compare the efficacy of multiple pheromone lures on several species of palm weevils. Here, we tested the commercial pheromone lure Dynalure (ferruginol) independently, and the lures Rhyncolure (rhynchophorol) and Metalure (89% ferruginol and 11% 2-methyl-4-heptanol) jointly, for their efficacy at attracting <i>R. palmarum</i> and <i>D. borassi</i> in a palm growing area of Tumaco, Colombia. We found that Dynalure and Rhyncolure are effective at attracting both species, but traps with Dynalure attract more <i>D. borassi</i>, while traps with Rhyncolure attract more <i>R. palmarum</i>. Metalure had a repellent effect for both species, highlighting the importance of minor components in pheromone lures. For <i>R. palmarum</i>, captures were female-biased in traps with the lures Rhyncolure and Metalure, while for <i>D. borassi</i> captures were male-biased with all lures tested. While both species showed similar numbers of captures per trap at the beginning of the experiment, at the end of the 14-month observation period, <i>R. palmarum</i> was the most abundant species captured in traps. Our results highlight the importance of evaluating the field efficacy of lures with different aggregation pheromones for mass trapping of palm weevils and to consider the effect of minor components in the lures, especially with the potential invasion of the red palm weevil to the Americas, and the urgent need to develop an integrated pest management programme that covers multiple species of sympatric palm weevils.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"185 2","pages":"264-273"},"PeriodicalIF":2.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141887275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological control of cat's claw creeper (Dolichandra unguis-cati; Bignoniaceae): Current status and future prospects","authors":"Kunjithapatham Dhileepan,&nbsp;Anthony M. King,&nbsp;Dianne B. J. Taylor,&nbsp;Kathryn M. Pollard,&nbsp;Marion K. Seier","doi":"10.1111/aab.12930","DOIUrl":"10.1111/aab.12930","url":null,"abstract":"<p>Cat's claw creeper (<i>Dolichandra unguis-cati</i>), native to tropical South America, is a major invasive species and a target for biological control in Australia, South Africa and some South Pacific Island countries. Native range surveys in Argentina, Brazil, Paraguay and Venezuela have identified eight insects and four fungal pathogens as potential agents. Five leaf-feeding insects a tortoise beetle <i>Charidotis auroguttata</i>, two tingids <i>Carvalhotingis visenda</i> and <i>C. hollandi</i>, a leaf-tying moth <i>Hypocosmia pyrochroma</i> and a leaf-mining beetle <i>Hedwigiella jureceki</i>, have been tested and all were released in South Africa. Four of these have become established but are not widespread and cause only limited damage. In Australia, only three of these, <i>C. visenda</i>, <i>H. pyrochroma</i> and <i>H. jureceki</i> were released, while <i>C. auroguttata</i> was not approved due to perceived non-target risks. All agents have become widely established, except for <i>H. pyrochroma</i> which is restricted to riparian corridors in southeast Queensland. In South Africa, an accidentally introduced leaf-spot pathogen, <i>Neoramulariopsis unguis-cati</i>, causes necrotic lesions and premature abscission of leaves in cat's claw creeper infestations, resulting in widespread defoliation. Based on its impact and field-host specificity in its native range and in South Africa, the pathogen has been prioritised for evaluation as a potential additional agent in Australia. The current priority is to seek approval for the introduction of this leaf-spot pathogen into Australia. Future research should focus on the gall-inducing rust <i>Uropyxis rickiana</i> and the seed-feeding weevil <i>Apteromechus notatus</i> as prospective agents.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"185 2","pages":"132-145"},"PeriodicalIF":2.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.12930","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141886595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil fauna changing in after-pine harvesting's areas along a temporal gradient of Atlantic Forest regeneration","authors":"Camila Elis Casaril,&nbsp;Luís Carlos Iuñes de Oliveira Filho,&nbsp;Mireli Moura Pitz Floriani,&nbsp;Dilmar Baretta,&nbsp;Osmar Klauberg-Filho","doi":"10.1111/aab.12932","DOIUrl":"10.1111/aab.12932","url":null,"abstract":"<p>Monoculture of pine alters the soil fauna community structure, but forest farms, mixed with natural areas, are restoring the original vegetation to protect, the diversity and ecosystem functions. In restoration initiatives it is necessary to consider the soil fauna. Thus, the aim of this study was to investigate how the process of restoration of the soil community occurs over time in areas after pine harvesting. Areas in natural regeneration with 4 and 10 years, pine plantations adjacent to these areas (representing previous use), and areas of natural forest (reference) were evaluated, it was evaluated three areas per treatment, with six sampling points spaced 30 m apart, totalling 18 sampling points for each treatment (<i>n</i> = 18). Soil mesofauna and macrofauna were collected through soil monoliths and pitfall traps. The comparison of the statistical analysis was to check for changes in the soil fauna over time (4 and 10 years) compared with that observed in secondary forest areas. The study showed that, along the natural regeneration time (4 and 10 years), it was observed increments in the diversity and evenness of the soil community and a reduction in the dominance of groups like Formicidae and increases in the community structure complexity with the resurgence of micropredators/regulators (Collembola and Acari), litter transformers (Oligochaeta, Diplopoda, Blattodea, Coleoptera (Larva), Diptera (Larva) and Coleoptera) and predators (Pseudoscorpiones, Opiliones, Symphyla and Coleoptera) after 10 years of natural regeneration, the areas have already recovered groups with important ecosystem functions, but are not yet at the level of abundance, functional diversity and community structure of native forest areas. The 4- and 10-year regeneration areas present major number of soil fauna groups of native forest (20 groups in regeneration areas and 17 in the native forest areas). Even so, the forest showed a major complexity of soil fauna community structure, evidenced by the diversity. This study contributes to the understanding of natural regeneration for soil fauna along a temporal gradient and these findings can guide the conservation of regional soil fauna diversity.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"185 3","pages":"383-395"},"PeriodicalIF":2.2,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141743858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uptake and transport of antibiotic kasugamycin from leaves to roots in rice (Oryza sativa L.) seedlings","authors":"Huayang Xu,&nbsp;Hongzhen Zhang,&nbsp;Guoyu Wei,&nbsp;Yuling Jiang,&nbsp;Meto Yao Charles,&nbsp;Anlong Hu","doi":"10.1111/aab.12928","DOIUrl":"10.1111/aab.12928","url":null,"abstract":"<p>Kasugamycin (KSM), an aminoglycoside antibiotic, has been widely used as a natural fungicide to control plant diseases, particularly for managing rice blast. However, its uptake mechanism and transport in rice remain to be explored. In this article, rice seedlings were treated by the foliar spraying method, and the content of KSM in rice leaves, stems and roots under different treatments was detected by high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS), respectively. Results showed that leaf-applied KSM could be transported to the phloem and migrate to roots and stems after uptake by leaves. Concentration, temperature and pH had significant effects on the uptake of KSM. Compared with the control, the competitive inhibitors <span>d</span>-glucose and phlorizin both inhibited the uptake of KSM, demonstrating that sugar transporter proteins were involved in the uptake process. The energy inhibitors dinitrophenol (DNP) and carbonyl cyanide chlorophenylhydrazone (CCCP) also significantly inhibited the uptake of KSM, indicating that the uptake of KSM required energy consumption. Thus, the uptake of KSM by rice was an active process involving sugar transporter proteins, and it could migrate downward through the phloem. This study contributes to the promotion of the scientific application of antibiotics and the biological control of crop diseases. It will also provide a theoretical basis for the development of root-targeted pesticides and transport pesticides with phloem mobility.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"185 3","pages":"360-370"},"PeriodicalIF":2.2,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141608541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A BBCH-scale code for the phenology of sand rice (Agriophyllum squarrosum (L.) Moq.)","authors":"Ruilan Ran,&nbsp;Guoxiong Chen,&nbsp;Pengshan Zhao","doi":"10.1111/aab.12924","DOIUrl":"10.1111/aab.12924","url":null,"abstract":"<p>Sand rice (<i>Agriophyllum squarrosum</i> (L.) Moq.), an annual psammophyte, is a promising wild plant for de novo domestication. Although many related studies have been documented recently, a basic phenological scale describing its growth stages is lacking. To address this gap, we have defined nine principal growth stages, each subdivided into several secondary stages according the international BBCH coding system. The growth stages mainly include seedling emergence, leaf development, formation of the primary branches, main stem elongation, inflorescence emergence, flowering, fruit development, ripening and senescence. Importantly, the growth sequence of sand rice reveals a distinctive stage, coded as BBCH21, characterized by the elongation of the first pair of primary branches, which occurs prior to main stem visible (BBCH30). This branch priority phenotype in sand rice strongly challenges the generally recognized growth pattern of ‘apical dominance’ in dicotyledons. The sequential and chronological progression of principal growth stages of sand rice was further determined in an experimental field with a representative genotype from the Tengger desert of Northwest China in 2023. The establishment of BBCH scale of sand rice lays a methodological foundation for morphological assessment among different germplasms and between wild-type and mutant plants. This will accelerate the de novo domestication process of sand rice using natural selection and artificial mutagenesis.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"185 3","pages":"323-332"},"PeriodicalIF":2.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141552279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive modelling of yellow stem borer population in rice using light trap: A comparative study of MLP and LSTM networks","authors":"Kiran Gandhi Bapatla,&nbsp;Basana Gowda Gadratagi,&nbsp;Naveenkumar B. Patil,&nbsp;Guru-Pirasanna Pandi Govindharaj,&nbsp;Lakshmi Narayana Thalluri,&nbsp;Bipin Bihari Panda","doi":"10.1111/aab.12927","DOIUrl":"10.1111/aab.12927","url":null,"abstract":"<p>The yellow stem borer (YSB), <i>Scirpophaga incertulas</i> (Walker), is a major insect pest that significantly damages rice crop. This study investigates methods to predict YSB populations in rice fields, aiming to develop an early warning system. Traditionally, rice farmers rely on light traps to monitor YSB presence. However, this study goes beyond this approach by combining light-trap data with weather information (temperature, humidity, rainfall) and utilizing powerful artificial intelligence (AI) techniques to forecast future YSB populations. Two AI methods, multilayer perceptron (MLP) and long short-term memory (LSTM), were employed to estimate YSB populations and assess their performance. The results revealed that the LSTM model outperformed the MLP model based on statistical metrics like RMSE, MAE, and <i>R</i>² values. Utilizing LSTM model with historical data, stakeholders in the Eastern Coastal Plains and Hills agro-climatic zone of India can gain a significant advantage in predicting YSB populations well in advance. This early warning system can alert stakeholders of potential YSB outbreaks, allowing them to take timely management actions and protect their rice crops from substantial yield losses.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"185 2","pages":"255-263"},"PeriodicalIF":2.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141531534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide study of OsWRKY gene in Oryza sativa subsp. japonica","authors":"Jhumishree Meher,&nbsp;Jeetu Narware,&nbsp;Prahlad Masurkar","doi":"10.1111/aab.12926","DOIUrl":"10.1111/aab.12926","url":null,"abstract":"<p>WRKY transcription factor (TF) family is well known to govern essential physiological functioning as well as regulate plant response to biotic and abiotic stress. In this study, we have identified 108 <i>OsWRKY</i> genes in the genome of <i>Oryza sativa</i> subsp. <i>japonica</i>, using the updated genomic data from the Rice Annotation Project Database and Oryzabase, which were further used to conduct the phylogenetic study, motif analysis, gene structure analysis, chromosomal mapping, and prediction of sub-cellular localization. The multiple sequence alignment OsWRKY proteins revealed the presence of nine different types of alterations in the conserved heptapeptide sequence WRKYGQK associated with 19 <i>OsWRKY</i> genes. Physiochemical analysis discloses the hydrophobic amino acid-rich, thermally stable, and polar nature of OsWRKY proteins. These genes were noted as highly conserved between the two cultivated sub-species of <i>Oryza sativa</i>, that is Indica and <i>japonica</i> type. Additionally, from motif analysis, we have found a new motif, which was categorized as hAT family C-terminal dimerization region associated with four members of group IIc. We have identified 21 stress-responsive <i>OsWRKY</i> genes, and their significance to the different biotic and abiotic stress-mediated cascades was further evaluated by analysing 1500 kb upstream sequences and this disclosed the presence of important phytohormone-responsive <i>cis</i>-elements in the OsWRKY gene, suggesting its direct involvement in defence against a wide range of external stressors and these 21 <i>OsWRKY</i> genes are tentatively listed as possible candidates for more study.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"185 3","pages":"345-359"},"PeriodicalIF":2.2,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141520518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crop age is the main driver affecting alfalfa mosaic virus: The predominant virus in the alfalfa virome","authors":"Roberto Meseguer,&nbsp;Alexandre Levi-Mourao,&nbsp;Eric Lucas,&nbsp;Xavier Pons,&nbsp;María Ángeles Achon","doi":"10.1111/aab.12929","DOIUrl":"10.1111/aab.12929","url":null,"abstract":"<p>The alfalfa virome has been understudied. Existing research dealing with viral incidences within the crop primarily concentrate on local factors, rather than considering a broader perspective. In this comprehensive 2-year study, we define the alfalfa virome and the main local and landscape factors affecting the incidence and annual increase of the Alfalfa mosaic virus (AMV), the most prevalent virus in alfalfa. The study was conducted in commercial alfalfa fields located along the highly productive northeast region of the Iberian Peninsula. For the first time in Europe, next-generation sequencing revealed the presence of 14 different viruses representing the genera <i>Cytorhabdovirus</i>, <i>Alphapartitivirus</i>, <i>Amalgavirus</i>, <i>Alfamovirus</i>, <i>Luteovirus</i>, <i>Enamovirus</i> and <i>Flavivirus</i>. AMV was the most prevalent species, accounting for 89% of the identified viral contigs. Enzyme-linked immunosorbent assays showed that the incidence of AMV varied between fields, with the average incidence doubling from 34% in 2019 to 65% in 2020. To assess the effect of local and landscape characteristics on the incidence of AMV and the observed annual increase, we selected different local variables and recorded landscape structure at three different buffer radii (250, 500 and 1000 m) from the centre of each field. Both the incidence and annual increase in AMV were driven mainly by local characteristics. The incidence of AMV showed a significant relationship with crop age and field area, whereas the annual increase was mainly influenced by crop age and the cumulative number of alate morphs of the aphid <i>Acyrthosiphon pisum</i>. Only one landscape composition variable, the percentage of alfalfa, showed a significant relationship with AMV incidence at the 250-m scale. These results confirm the effect of local variables on the population structure of generalist viruses.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"185 3","pages":"371-382"},"PeriodicalIF":2.2,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.12929","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141552304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}