Genome‐wide study of OsWRKY gene in Oryza sativa subsp. japonica

IF 2.2 3区农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY

Annals of Applied Biology Pub Date : 2024-07-01 DOI:10.1111/aab.12926

Jhumishree Meher, Jeetu Narware, Prahlad Masurkar

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引用次数: 0

Abstract

WRKY transcription factor (TF) family is well known to govern essential physiological functioning as well as regulate plant response to biotic and abiotic stress. In this study, we have identified 108 OsWRKY genes in the genome of Oryza sativa subsp. japonica, using the updated genomic data from the Rice Annotation Project Database and Oryzabase, which were further used to conduct the phylogenetic study, motif analysis, gene structure analysis, chromosomal mapping, and prediction of sub‐cellular localization. The multiple sequence alignment OsWRKY proteins revealed the presence of nine different types of alterations in the conserved heptapeptide sequence WRKYGQK associated with 19 OsWRKY genes. Physiochemical analysis discloses the hydrophobic amino acid‐rich, thermally stable, and polar nature of OsWRKY proteins. These genes were noted as highly conserved between the two cultivated sub‐species of Oryza sativa, that is Indica and japonica type. Additionally, from motif analysis, we have found a new motif, which was categorized as hAT family C‐terminal dimerization region associated with four members of group IIc. We have identified 21 stress‐responsive OsWRKY genes, and their significance to the different biotic and abiotic stress‐mediated cascades was further evaluated by analysing 1500 kb upstream sequences and this disclosed the presence of important phytohormone‐responsive cis‐elements in the OsWRKY gene, suggesting its direct involvement in defence against a wide range of external stressors and these 21 OsWRKY genes are tentatively listed as possible candidates for more study.

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Oryza sativa subsp. japonica 中 OsWRKY 基因的全基因组研究

众所周知，WRKY 转录因子（TF）家族调控植物的基本生理功能，并调节植物对生物和非生物胁迫的反应。本研究利用水稻注释项目数据库（Rice Annotation Project Database）和Oryzabase的最新基因组数据，鉴定了Oryza sativa subsp.多序列比对 OsWRKY 蛋白发现，与 19 个 OsWRKY 基因相关的保守七肽序列 WRKYGQK 存在 9 种不同类型的改变。理化分析表明，OsWRKY 蛋白富含疏水氨基酸，具有热稳定性和极性。这些基因在籼型和粳型两个栽培亚种之间高度保守。此外，通过基团分析，我们发现了一个新基团，它被归类为与 IIc 组四个成员相关的 hAT 家族 C 端二聚化区域。通过分析上游 1500 kb 的序列，我们发现 OsWRKY 基因中存在重要的植物激素响应顺式元件，这表明该基因直接参与了抵御各种外部胁迫的过程。

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来源期刊

Annals of Applied Biology 生物-农业综合

CiteScore

5.50

自引率

0.00%

发文量

审稿时长

18-36 weeks

期刊介绍： Annals of Applied Biology is an international journal sponsored by the Association of Applied Biologists. The journal publishes original research papers on all aspects of applied research on crop production, crop protection and the cropping ecosystem. The journal is published both online and in six printed issues per year. Annals papers must contribute substantially to the advancement of knowledge and may, among others, encompass the scientific disciplines of: Agronomy Agrometeorology Agrienvironmental sciences Applied genomics Applied metabolomics Applied proteomics Biodiversity Biological control Climate change Crop ecology Entomology Genetic manipulation Molecular biology Mycology Nematology Pests Plant pathology Plant breeding & genetics Plant physiology Post harvest biology Soil science Statistics Virology Weed biology Annals also welcomes reviews of interest in these subject areas. Reviews should be critical surveys of the field and offer new insights. All papers are subject to peer review. Papers must usually contribute substantially to the advancement of knowledge in applied biology but short papers discussing techniques or substantiated results, and reviews of current knowledge of interest to applied biologists will be considered for publication. Papers or reviews must not be offered to any other journal for prior or simultaneous publication and normally average seven printed pages.