Applied Biosafety最新文献

{"title":"Decontamination of <i>Bacillus</i> Spores with Formaldehyde Vapor under Varied Environmental Conditions.","authors":"Young W Choi, Michelle M Sunderman, Martha W McCauley, William R Richter, Zachary J Willenberg, Joseph Wood, Shannon Serre, Leroy Mickelsen, Stuart Willison, Rich Rupert, Jorge G Muñiz-Ortiz, Sara Casey, M Worth Calfee","doi":"10.1089/apb.21.926975","DOIUrl":"10.1089/apb.21.926975","url":null,"abstract":"<p><strong>Introduction: </strong>This study investigated formaldehyde decontamination efficacy against dried <i>Bacillus</i> spores on porous and non-porous test surfaces, under various environmental conditions. This knowledge will help responders determine effective formaldehyde exposure parameters to decontaminate affected spaces following a biological agent release.</p><p><strong>Methods: </strong>Prescribed masses of paraformaldehyde or formalin were sublimated or evaporated, respectively, to generate formaldehyde vapor within a bench-scale test chamber. Adsorbent cartridges were used to measure formaldehyde vapor concentrations in the chamber at pre-determined times. A validated method was used to extract the cartridges and analyze for formaldehyde via liquid chromatography. Spores of <i>Bacillus globigii</i>, <i>Bacillus thuringiensis</i>, and <i>Bacillus anthracis</i> were inoculated and dried onto porous bare pine wood and non-porous painted concrete material coupons. A series of tests was conducted where temperature, relative humidity, and formaldehyde concentration were varied, to determine treatment efficacy outside of conditions where this decontaminant is well-characterized (laboratory temperature and humidity and 12 mg/L theoretical formaldehyde vapor concentration) to predict decontamination efficacy in applications that may arise following a biological incident.</p><p><strong>Results: </strong>Low temperature trials (approximately 10°C) resulted in decreased formaldehyde air concentrations throughout the 48-hour time-course when compared with formaldehyde concentrations collected in the ambient temperature trials (approximately 22°C). Generally, decontamination efficacy on wood was lower for all three spore types compared with painted concrete. Also, higher recoveries resulted from painted concrete compared to wood, consistent with historical data on these materials. The highest decontamination efficacies were observed on the spores subjected to the longest exposures (48 hours) on both materials, with efficacies that gradually decreased with shorter exposures. Adsorption or absorption of the formaldehyde vapor may have been a factor, especially during the low temperature trials, resulting in less available formaldehyde in the air when measured.</p><p><strong>Conclusion: </strong>Environmental conditions affect formaldehyde concentrations in the air and thereby affect decontamination efficacy. Efficacy is also impacted by the material with which the contaminants are in contact.</p>","PeriodicalId":7962,"journal":{"name":"Applied Biosafety","volume":"26 3","pages":"139-53"},"PeriodicalIF":0.5,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511015/pdf/apb.21.926975.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9101747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Room Temperature Wait and Reuse for Bioburden Reduction of SARS-CoV-2 on N95 Filtering Facepiece Respirators.","authors":"Sylvia J Smullin,&nbsp;Branden D Tarlow","doi":"10.1089/apb.20.0055","DOIUrl":"https://doi.org/10.1089/apb.20.0055","url":null,"abstract":"<p><p><b>Introduction:</b> During a pandemic, when the supply of N95 filtering facepiece respirators (FFRs) is limited, health care workers may reuse N95 FFRs. Room temperature storage of N95 FFRs-waiting before reuse-could be a simple low-cost method to reduce severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) bioburden in such a situation. The U.S. Centers for Disease Control and Prevention specify this as a strategy for reducing self-contamination risk during a time of N95 FFR shortage. <b>Objective:</b> To review the literature on persistence of SARS-CoV-2 on surfaces to assess room temperature waiting times for bioburden reduction on N95 FFRs. <b>Methods:</b> The literature was searched for studies evaluating room temperature persistence of SARS-CoV-2. A 3-log decay time was extracted from published data for quantitative comparison between different studies. Studies using surgical masks and non-peer-reviewed studies that include N95 FFRs were used to draw conclusions. <b>Key Findings:</b> Experimental and analytical choices vary between studies and impact the estimated 3-log decay time. There is not a clear understanding of which material properties are significant. There are no peer-reviewed studies of virus persistence on an N95 FFR. <b>Discussion and Conclusions:</b> SARS-COV-2 inactivation occurs spontaneously at room temperature. The precise timing depends on factors including humidity, temperature, and surface material. In reviewed studies, a 7-day waiting period encompasses the 3-log reduction in infectious titer of SARS-COV-2 on specific N95 FFRs and surgical masks. Owing to variations between studies and among N95 FFR materials and room temperature conditions, it is impossible to extrapolate from these limited data to assign a precise 3-log decay time for all used N95 FFRs.</p>","PeriodicalId":7962,"journal":{"name":"Applied Biosafety","volume":"26 2","pages":"103-111"},"PeriodicalIF":1.5,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134324/pdf/apb.20.0055.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10255756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen Peroxide Methods for Decontaminating N95 Filtering Facepiece Respirators.","authors":"David Rempel,&nbsp;John Henneman,&nbsp;James Agalloco,&nbsp;Jill Crittenden","doi":"10.1089/apb.20.0042","DOIUrl":"https://doi.org/10.1089/apb.20.0042","url":null,"abstract":"<p><p><b>Introduction:</b> During a pandemic, when the supply of N95 filtering facepiece respirators (FFRs) is limited, FFRs may be decontaminated by methods that inactivate pathogens as long as they do not damage FFR function. Hydrogen peroxide (H₂O₂) is widely used for decontamination in medical settings. <b>Objective:</b> To review the literature on the use of H₂O₂ to decontaminate N95 FFRs and identify methods that inactivate virus and preserve FFR filtration efficiency and fit. <b>Methods:</b> The literature was searched for studies evaluating H₂O₂ decontamination methods on inactivating SARS-CoV-2 and other viruses and microorganisms inoculated on N95 FFRs and the effects on respirator filtration efficiency and fit. Current U.S. Federal guidelines are also presented. <b>Results:</b> Findings from relevant laboratory studies (<i>N</i> = 24) are summarized in tables. Commercially available H₂O₂ decontamination systems differ on how H₂O₂ is delivered, the temperature, the duration of treatment, and other factors that can impact N95 FFR filtration efficiency and fit. Some methods inactivate SARS-CoV-2 virus-contaminated N95 FFRs with >3 log attenuation, whereas other methods are yet to be evaluated. <b>Discussion and Conclusion:</b> Most of the H₂O₂ methods reviewed effectively decontaminate N95 FFRs without damaging FFR function. However, some methods adversely impact N95 fit or filtration efficiency, which could go undetected by the end user and compromise their protection from pathogen inhalation. When making decisions about H₂O₂ decontamination of respirators, it is important to understand differences in methods, effects on different FFR models, and potential hazards to workers who manage the decontamination process.</p>","PeriodicalId":7962,"journal":{"name":"Applied Biosafety","volume":"26 2","pages":"71-79"},"PeriodicalIF":1.5,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134325/pdf/apb.20.0042.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9532517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Evaluation of Single-Use Particle Filtering Half Masks for SARS-CoV-2 Protection.","authors":"Lillemor Örebrand,&nbsp;Max Bäckman,&nbsp;Oscar Björnham,&nbsp;Marianne Thunéll,&nbsp;Andreas Fredman,&nbsp;Niklas Brännström","doi":"10.1089/apb.2020.0082","DOIUrl":"https://doi.org/10.1089/apb.2020.0082","url":null,"abstract":"<p><p><b>Background:</b> The SARS-CoV-2 pandemic put the entire healthcare sector under severe strain due to shortages of personal protection equipment. A large number of new filtering mask models were introduced on the market, claiming effectiveness that had undergone little or no objective and reliable verifications. <b>Methods and Materials:</b> Filter materials were tested against sodium chloride particles according to the EN149 §7.9.2 standard for particle penetration. Particle counters were used to measure the particle penetration of the filtering mask models, resolved over sizes in the range of 27-1000 nm. <b>Results:</b> We report on the results for 86 different filtering mask models. The majority of the tested models showed <3% penetration, whereas almost one third (i.e., 27 of 86) of the models performed poorly. <b>Discussion:</b> Interestingly, the poorest performing masks showed a tendency to have worse filtering effectiveness for larger particles than for smaller sized particles, following the opposite tendency of the best filtering masks. <b>Conclusion:</b> Almost one third of the filtering mask models tested failed the specified pass criteria as specified in the temporary EU COVID-19 standard. This fact, and the high health risks of COVID-19, highlights the need for independent testing.</p>","PeriodicalId":7962,"journal":{"name":"Applied Biosafety","volume":"26 2","pages":"58-65"},"PeriodicalIF":1.5,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/83/fa/apb.2020.0082.PMC9134329.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10248471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applied Biosafety's Themed Issue on Methods for Testing, Decontaminating, and Reducing Bioburden on Respiratory Protective Devices and Filters","authors":"Barbara Johnson, K. Byers, S. Patlovich, David R Gillum","doi":"10.1089/APB.2021.29000.INTRO","DOIUrl":"https://doi.org/10.1089/APB.2021.29000.INTRO","url":null,"abstract":"","PeriodicalId":7962,"journal":{"name":"Applied Biosafety","volume":"26 1","pages":"57-57"},"PeriodicalIF":1.5,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49536173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formaldehyde Vapor Characteristics in Varied Decontamination Environments.","authors":"Young W Choi, Michelle M Sunderman, Martha W McCauley, William R Richter, Zachary J Willenberg, Joseph Wood, Shannon Serre, Leroy Mickelsen, Stuart Willison, Rich Rupert, Jorge G Muñiz Ortiz, Sara Casey, M Worth Calfee","doi":"10.1089/apb.21.926968","DOIUrl":"10.1089/apb.21.926968","url":null,"abstract":"<p><strong>Introduction: </strong>This effort investigated formaldehyde vapor characteristics under various environmental conditions by the analyses of air samples collected over a time-course. This knowledge will help responders achieve desired formaldehyde exposure parameters for decontamination of affected spaces after a biological contamination incident.</p><p><strong>Methods: </strong>Prescribed masses of paraformaldehyde and formalin were sublimated or evaporated, respectively, to generate formaldehyde vapor. Adsorbent cartridges were used to collect air samples from the test chamber at predetermined times. A validated method was used to extract the cartridges and analyze for formaldehyde via liquid chromatography. In addition, material demand for the formaldehyde was evaluated by inclusion of arrays of Plexiglas panels in the test chamber to determine the impact of varied surface areas within the test chamber. Temperature was controlled with a circulating water bath connected to a radiator and fan inside the chamber. Relative humidity was controlled with humidity fixed-point salt solutions and water vapor generated from evaporated water.</p><p><strong>Results: </strong>Low temperature trials (approximately 10°C) resulted in decreased formaldehyde air concentrations throughout the 48-hour time-course when compared with formaldehyde concentrations in the ambient temperature trials (approximately 22°C). The addition of clear Plexiglas panels to increase the surface area of the test chamber interior resulted in appreciable decreases of formaldehyde air concentration when compared to an empty test chamber.</p><p><strong>Conclusion: </strong>This work has shown that environmental variables and surface-to-volume ratios in the decontaminated space may affect the availability of formaldehyde in the air and, therefore, may affect decontamination effectiveness.</p>","PeriodicalId":7962,"journal":{"name":"Applied Biosafety","volume":"26 1","pages":"33-41"},"PeriodicalIF":0.5,"publicationDate":"2021-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129920/pdf/apb.21.926968.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38921667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decontamination of <i>Bacillus anthracis</i> Spores at Subzero Temperatures by Complete Submersion.","authors":"Chad Laing,&nbsp;Timothy Janzen,&nbsp;Vladimir Blinov,&nbsp;Konstantin Volchek,&nbsp;Noriko Goji,&nbsp;Matthew Thomas,&nbsp;Melissa Telfer,&nbsp;Elizabeth Rohonczy,&nbsp;Kingsley K Amoako","doi":"10.1089/apb.20.0067","DOIUrl":"https://doi.org/10.1089/apb.20.0067","url":null,"abstract":"<p><p><b>Introduction:</b> <i>Bacillus anthracis</i>, the etiological agent of anthrax, produces long-lived spores, which are resistant to heat, cold, pH, desiccation, and chemical agents. The spores maintain their ability to produce viable bacteria even after decades, and when inhaled can cause fatal disease in over half of the clinical cases. Owing to these characteristics, anthrax has been repeatedly selected for both bioweapon and bioterrorism use. In the event of a bioterrorism attack, surfaces in the vicinity of the attack will be contaminated, and recovering from such an event requires rapid and effective decontamination. Previous decontamination method development has focused mainly on temperatures >0°C, and have shown poor efficacy at subzero temperatures. <b>Methods:</b> In this study, we demonstrate the use of calcium chloride (CaCl₂) as a freezing point depression agent for pH-adjusted sodium hypochlorite (NaOCl) for the effective and rapid decontamination of <i>B. anthracis</i> Sterne strain spores at subzero temperatures. <b>Results:</b> We show the complete decontamination of 10⁶ <i>B. anthracis</i> Sterne strain spores at temperatures as low as -20°C within 2.5 min by submersion in solution containing 25% (w/v) CaCl₂, 0.50% NaOCl, and 0.40% (v/v) acetic acid. We also demonstrate significant reduction in number of spores at -28°C. <b>Conclusions:</b> The results show promise for rapidly decontaminating equipment and materials used in the response to bioterrorism events using readily available consumer chemicals. Future study should examine the efficacy of these results on complex surfaces.</p>","PeriodicalId":7962,"journal":{"name":"Applied Biosafety","volume":"26 1","pages":"6-13"},"PeriodicalIF":1.5,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869638/pdf/apb.20.0067.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33442785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological Validation of a Chemical Effluent Decontamination System.","authors":"Christopher K Cote,&nbsp;Jessica M Weidner,&nbsp;Christopher Klimko,&nbsp;Ashley E Piper,&nbsp;Jeremy A Miller,&nbsp;Melissa Hunter,&nbsp;Jennifer L Shoe,&nbsp;Jennifer C Hoover,&nbsp;Brian R Sauerbry,&nbsp;Tony Buhr,&nbsp;Joel A Bozue,&nbsp;David E Harbourt,&nbsp;Pamela J Glass","doi":"10.1089/apb.21.937967","DOIUrl":"https://doi.org/10.1089/apb.21.937967","url":null,"abstract":"<p><p><b>Introduction:</b> Failure of an existing effluent decontamination system (EDS) prompted the consideration of commercial off-the-shelf solutions for decontamination of containment laboratory waste. A bleach-based chemical EDS was purchased to serve as an interim solution. <b>Methods:</b> Studies were conducted in the laboratory to validate inactivation of <i>Bacillus</i> spores with bleach in complex matrices containing organic simulants including fetal bovine serum, humic acid, and animal room sanitation effluent. <b>Results:</b> These studies demonstrated effective decontamination of >10⁶ spores at a free chlorine concentration of ≥5700 parts per million with a 2-hour contact time. Translation of these results to biological validation of the bleach-based chemical EDS required some modifications to the system and its operation. <b>Discussion:</b> The chemical EDS was validated for the treatment of biosafety levels 3 and 4 waste effluent using laboratory-prepared spore packets along with commercial biological indicators; however, several issues and lessons learned identified during the process of onboarding are also discussed, including bleach product source, method of validation, dechlorination, and treated waste disposal.</p>","PeriodicalId":7962,"journal":{"name":"Applied Biosafety","volume":"26 1","pages":"23-32"},"PeriodicalIF":1.5,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869648/pdf/apb.21.937967.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33442786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Hitchhiker's Guide to Hydrogen Peroxide Fumigation, Part 2: Verifying and Validating Hydrogen Peroxide Fumigation Cycles.","authors":"Daniel Kümin, Monika Gsell Albert, Benjamin Weber, Kathrin Summermatter","doi":"10.1089/apb.21.921099","DOIUrl":"10.1089/apb.21.921099","url":null,"abstract":"<p><p><b>Introduction:</b> Part 1 of this two-part series describes the use of hydrogen peroxide as a fumigant and compares it with other fumigants on the market. Technical requirements are outlined while considering physical and biological limitations of the system. This second part focuses primarily on the use of process controls to verify and validate hydrogen peroxide fumigations. Finally, a model encompassing the entire fumigation process is presented. <b>Methods:</b> Part 2 of the series focuses on the authors' long-time personal experiences in room and filter fumigation using various fumigation systems and is supplemented with relevant literature searches. <b>Results:</b> The reader is introduced to the planning and implementation of fumigation process validations. Biological indicators help users develop safe and efficient processes. Chemical indicators can be used as process controls, while measuring physical parameters will help avoid condensation of hydrogen peroxide. How many biological and chemical indicators and what type should be applied for cycle development are additionally explained. <b>Discussion:</b> It is important to consider numerous technical requirements when planning to implement hydrogen peroxide fumigation at an institution. Also, considerable thought needs to go into the verification and validation of the fumigation process. <b>Conclusions:</b> Part 1 of this series presents an overview of different fumigation systems based on hydrogen peroxide on the market and their technical requirements. Part 2 focuses on validation and verification of hydrogen peroxide fumigation while considering the entire fumigation process. The two parts together will serve users as a guide to establishing hydrogen peroxide fumigations at their facilities.</p>","PeriodicalId":7962,"journal":{"name":"Applied Biosafety","volume":"26 1","pages":"42-51"},"PeriodicalIF":0.5,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869643/pdf/apb.21.921099.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33443247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Technical and Anatomical Considerations for Reproducible Inactivation of Large Animal Carcasses by Steam Sterilization.","authors":"Jan Schinköthe, Benjamin Bartram-Sitzius, Jens-Peter Teifke, Ute Pfitzner, Sven Reiche","doi":"10.1089/apb.21.919637","DOIUrl":"10.1089/apb.21.919637","url":null,"abstract":"<p><p><b>Introduction:</b> The complete inactivation of infectious tissues of large animal carcasses is one of the most challenging tasks in high-containment facilities. Steam sterilization is a method frequently in use to achieve biological inactivation of liquid and solid waste. <b>Objective:</b> This study aims to highlight parameters most effective in creating reproducible cycles for steam sterilization of pig and calf carcasses. <b>Methods:</b> Two pigs or 1 calf were sterilized by running a liquid cycle (n = 3) at 121°C for at least 120 minutes in a pass-through autoclave. To assess the physical and biological parameters, temperature data loggers and biological indicators (BIs) with spores of <i>Geobacillus stearothermophilus</i> (ATCC 7953) were placed at defined positions within animal carcasses. After completion of each cycle, data loggers were analyzed and BIs were incubated for 7 days at 60°C. <b>Results:</b> Initial testing with an undissected pig carcass resulted in suboptimal temperatures at the tissue level with growth on 1 BI. After modifications of the used stainless-steel boxes and by placing the reference probe of the autoclave in the animal carcass, reproducible cycles could be created. A complete inactivation of BIs and a temperature profile of >121°C for at least 20 minutes could be achieved in almost all probed tissues. <b>Conclusion:</b> Only minor modifications in carcass preparation and the used sterilization equipment resulted in effective and reproducible cycles to inactivate large animal carcasses by using a steam autoclave.</p>","PeriodicalId":7962,"journal":{"name":"Applied Biosafety","volume":"26 1","pages":"14-22"},"PeriodicalIF":0.5,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869644/pdf/apb.21.919637.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33442788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}