Animal Cells and Systems最新文献_第7页

TRIM22 functions as a scaffold protein for autophagy initiation. TRIM22作为自噬启动的支架蛋白。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2025-05-06 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2498926

Hyungsun Park, Hansol Heo, Yeongseo Song, Myung Shin Lee, Yebin Cho, Jae-Seon Lee, Jaerak Chang, Seongju Lee

{"title":"TRIM22 functions as a scaffold protein for autophagy initiation.","authors":"Hyungsun Park, Hansol Heo, Yeongseo Song, Myung Shin Lee, Yebin Cho, Jae-Seon Lee, Jaerak Chang, Seongju Lee","doi":"10.1080/19768354.2025.2498926","DOIUrl":"https://doi.org/10.1080/19768354.2025.2498926","url":null,"abstract":"Tripartite motif (TRIM) family proteins are increasingly recognized as important regulators of autophagy under various physiological and pathological conditions. TRIM22 has been previously shown to mediate autophagosome-lysosome fusion, but its potential role in earlier stages of autophagy remained unexplored. In this study, we investigated the function of TRIM22 in autophagy initiation. Overexpression of TRIM22 increased LC3-II levels and enhanced autophagic flux without affecting mTOR and AMPK activity. We found that TRIM22 interacts with components of both the ULK1 complex and the class III PI3K complex through distinct domains, recruiting them into punctate structures that represent autophagosome formation sites. Domain mapping revealed that the SPRY domain mediates interactions with ATG13 and FIP200, while the N-terminal region interacts with ULK1 and ATG101. The B-box domain of TRIM22 was identified as crucial for its interaction with Beclin-1, a key component of the class III PI3K complex. Deletion of this domain impaired the ability of TRIM22 to assemble the class III PI3K complex and induce autophagic flux. Interestingly, competitive binding assays revealed that Beclin-1 and PLEKHM1 bind to the same region of TRIM22, suggesting a mechanism for coordinating different stages of autophagy. The Alzheimer's disease-associated TRIM22 variant R321K maintained autophagy initiation function in both cell lines and primary neurons. These findings demonstrate that TRIM22 acts as a scaffold protein to promote autophagy initiation, in addition to its previously described role in autophagosome-lysosome fusion. Our study provides new insights into the molecular mechanisms by which TRIM proteins regulate multiple stages of the autophagy process.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"296-311"},"PeriodicalIF":2.5,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early postnatal exposure to bicuculline modulates E/I balance and induces ASD-like behavioral phenotypes in mice. 出生后早期暴露于双核碱可调节小鼠E/I平衡并诱导asd样行为表型。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2025-04-28 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2493258

Dongpil Shin, Eunbi Cho, Kwanghoon Park, ChiHye Chung, Dong Hyun Kim, Se Jin Jeon, Chan Young Shin

{"title":"Early postnatal exposure to bicuculline modulates E/I balance and induces ASD-like behavioral phenotypes in mice.","authors":"Dongpil Shin, Eunbi Cho, Kwanghoon Park, ChiHye Chung, Dong Hyun Kim, Se Jin Jeon, Chan Young Shin","doi":"10.1080/19768354.2025.2493258","DOIUrl":"10.1080/19768354.2025.2493258","url":null,"abstract":"Autism spectrum disorder (ASD) is a neurodevelopmental condition characterized by social interaction deficits and repetitive behaviors. While precise causes of ASD remain elusive, growing evidence highlights that an imbalance in excitatory and inhibitory (E/I) signaling is a pivotal factor in ASD development and modulation. Balanced E/I neurotransmission is critical for circuit formation, synaptic plasticity, and developmental timing. However, key questions persist, including the critical perturbation window, neurological and neurodevelopmental effects, and clinical implications of E/I imbalance. This study investigated early-life modulation of the GABAergic system's impact on E/I balance and ASD-like behaviors in mice. Mice were treated with bicuculline, a GABAA receptor antagonist, from postnatal days 7-11, and behavioral tests were conducted during adolescence. Results revealed deficits in social interaction in both male and female mice and increased repetitive behaviors in bicuculline-treated male mice. Electrophysiological recordings in the mPFC indicated reduced resting membrane potential, heightened neuronal excitability, and a shift in the E/I ratio. In the hippocampus, recordings displayed enhanced LTP and altered synaptic plasticity. DEG analysis of the PFC in bicuculline-treated mice unveiled aberrant gene profiles related to the regulation of synaptic function. Clinical significance and underlying mechanisms of abnormal brain activity, neurodevelopment, and ASD-related behaviors prompted by neonatal bicuculline treatment require further investigation. Nevertheless, these results suggest that GABAergic signaling disruption during the neonatal period might contribute to ASD-related brain pathophysiological changes.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"264-281"},"PeriodicalIF":2.5,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12124418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling ER dimerization dynamics in endocrine disruption based on a BRET-focused approach. 基于bret聚焦方法揭示内分泌干扰中的内质网二聚化动力学。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2025-04-28 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2481984

Soomin Yum, Haksoo Lee, Yong-Kook Kwon, Gunyoung Lee, Hye-Young Lee, HyeSook Youn, BuHyun Youn

{"title":"Unraveling ER dimerization dynamics in endocrine disruption based on a BRET-focused approach.","authors":"Soomin Yum, Haksoo Lee, Yong-Kook Kwon, Gunyoung Lee, Hye-Young Lee, HyeSook Youn, BuHyun Youn","doi":"10.1080/19768354.2025.2481984","DOIUrl":"https://doi.org/10.1080/19768354.2025.2481984","url":null,"abstract":"Endocrine-disrupting chemicals (EDCs) are exogenous compounds that interact with the estrogen receptor (ER), thereby disrupting estrogen-mediated signaling. In a previous study, we employed a bioluminescence resonance energy transfer (BRET) system to assess ER dimerization for detecting EDCs. To further determine whether the BRET assay could be used independently to identify EDCs, we investigated ER-EDC interactions before and after dimerization. Results from isothermal titration calorimetry (ITC) and dynamic light scattering (DLS) revealed that ER dimerization can be mediated by EDCs. Consequently, the BRET assay proved effective in detecting dimerization and clarifying its relevance to EDC-induced signaling disruption. Additionally, to examine EDC-induced transcriptional changes, we performed chromatin immunoprecipitation sequencing (ChIP-seq), followed by gene ontology (GO) analysis. These analyses demonstrated that EDCs affect various signaling pathways, including those involved in antibody-dependent cytotoxicity, bone morphogenetic protein (BMP) signaling in cardiac induction, and hepatocyte growth factor receptor signaling. Overall, this study elucidates the molecular mechanisms by which EDCs influence ER dimerization and signaling. These findings highlight the utility of the BRET-based assay for EDC detection and contribute to a deeper understanding of the systemic effects of EDCs on endocrine disruption.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"282-295"},"PeriodicalIF":2.5,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12039421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of an in vitro erythroid differentiation system from canine peripheral blood mononuclear cells. 犬外周血单个核细胞体外红细胞分化系统的建立。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2025-04-28 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2492148

Sung Ah Park, Arim Shin, Eunji Im, Do Hyeon Yu, Minji Kim, Chul Geun Kim, Eun Jung Baek

{"title":"Establishment of an in vitro erythroid differentiation system from canine peripheral blood mononuclear cells.","authors":"Sung Ah Park, Arim Shin, Eunji Im, Do Hyeon Yu, Minji Kim, Chul Geun Kim, Eun Jung Baek","doi":"10.1080/19768354.2025.2492148","DOIUrl":"https://doi.org/10.1080/19768354.2025.2492148","url":null,"abstract":"Blood transfusion is a critical, lifesaving medical procedure for dogs. However, the limited availability of blood donors and ethical concerns highlight the need for alternative solutions, such as in vitro-produced red blood cells (RBCs), which remain unexplored in canines. This study aimed to produce canine erythrocytes in vitro from peripheral blood (PB) mononuclear cells (MNCs), optimize culture conditions using either human or canine reagents, and identify relevant cell markers. Results indicated that canine erythropoiesis can be induced by human or canine cytokines, producing RBCs within approximately 20 days. Although cell numbers decreased during the first seven days, immature erythroid cells proliferated, reaching peak expansion and RBC production by day 17. Despite the smaller cell size of the cultured RBCs than that of humans, the morphology at each stage of erythroid maturation was analogous to that of human erythropoiesis. Furthermore, the expression patterns of canine alpha hemoglobin stabilizing protein and erythropoietin receptor mirrored those observed in human erythropoiesis. Oxygen-hemoglobin (oxygen-Hb) association and dissociation curves of cultured RBCs closely resembled those of native canine RBCs, indicating an appropriate oxygen-carrying capacity. This study presents the first evidence of successful in vitro production of canine RBCs, offering a promising tool for research and potential therapeutic applications.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"251-263"},"PeriodicalIF":2.5,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12039424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144061400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asxl1 loss in mice leads to microcephaly by regulating neural stem cell survival. 小鼠中Asxl1缺失通过调节神经干细胞存活导致小头畸形。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2025-04-23 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2481979

Hyeju Kim, A-Reum Kim, Sukyoung Byun, Soo-Jong Um

{"title":"Asxl1 loss in mice leads to microcephaly by regulating neural stem cell survival.","authors":"Hyeju Kim, A-Reum Kim, Sukyoung Byun, Soo-Jong Um","doi":"10.1080/19768354.2025.2481979","DOIUrl":"https://doi.org/10.1080/19768354.2025.2481979","url":null,"abstract":"Additional sex comb-like 1 (ASXL1) is a chromatin-associated factor essential for transcriptional regulation. De novo truncating mutations in the ASXL1 gene are linked to Bohring-Opitz syndrome, a developmental disorder characterized by microcephaly; however, the role of Asxl1 in brain development remains unclear. In this study, we demonstrate that Asxl1 deletion in mice induces microcephaly, primarily caused by a reduction in the size and number of cortical neurons. Asxl1 ablation disrupts neural stem cell (NSC) maintenance, as evidenced by decreased proliferation and increased apoptosis. Transcriptomic analysis of Asxl1-deficient NSCs revealed 4,635 differentially expressed genes, including 2,262 upregulated and 2,373 downregulated genes. Gene ontology analysis indicated that Asxl1 regulates NSC survival through the histone methyltransferase Ezh2, a core component of the Polycomb Repressive Complex 2 (PRC2). Inhibition of H3K27me3 using GSK343 significantly reduced the viability of wild-type NSCs, but had a markedly diminished effect on Asxl1-deficient NSCs. Furthermore, Ezh2 target genes associated with apoptosis, such as Epha7 and Osr1, were upregulated in wild-type NSCs following GSK343 treatment but not significantly affected in Asxl1-deficient NSCs. These findings establish Asxl1 as a critical regulator of NSC survival and neurogenesis via Ezh2-mediated chromatin modification and provide insights into the mechanisms underlying microcephaly in developmental disorders.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"241-250"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-read sequencing reveals novel transcript diversity in Polypterus senegalus. 长读测序揭示了塞内加尔多叶蛇新的转录多样性。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2479057

Jeong Woen Shin, Bo-Mi Kim, Seung Hwan Lee, Jun Kim, Jae-Sung Rhee

{"title":"Long-read sequencing reveals novel transcript diversity in Polypterus senegalus.","authors":"Jeong Woen Shin, Bo-Mi Kim, Seung Hwan Lee, Jun Kim, Jae-Sung Rhee","doi":"10.1080/19768354.2025.2479057","DOIUrl":"10.1080/19768354.2025.2479057","url":null,"abstract":"Polypterus senegalus is a fish species characterized by primitive traits and unique physiological-anatomical features, representing a transitional stage toward terrestrial vertebrates. Diverging from other ray-finned fishes approximately 420 million years ago, it serves as a key model for vertebrate evolutionary studies. In this study, we identified 19,295 unique P. senegalus transcripts using Pacific Biosciences Iso-Seq, with most classified as novel exon combinations, intron retentions, or novel start and stop codons. This high-quality, full-length transcriptome revealed previously unreported isoforms and investigated their functional roles to enhance our understanding of the genomic complexity and evolutionary traits of the species. Notably, these isoforms were identified in genes associated with Polypterus's distinctive features, including limb flexibility (ACTN3B, MYBPC1), lung function (FAM13A, ERBB2, TCF7), and circulation (FBN1, MYH11B, MEF2CB). These findings offer molecular insights into early vertebrate terrestrial adaptation and the functional integration of key tissues in extant species.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"643-652"},"PeriodicalIF":3.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12581780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145443727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative epigenomics to clinical trials in human breast cancer and canine mammary tumor. 比较表观基因组学在人类乳腺癌和犬乳腺肿瘤临床试验中的应用。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2025-03-19 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2477024

Su-Jin Jeong, Kang-Hoon Lee, Je-Yoel Cho

{"title":"Comparative epigenomics to clinical trials in human breast cancer and canine mammary tumor.","authors":"Su-Jin Jeong, Kang-Hoon Lee, Je-Yoel Cho","doi":"10.1080/19768354.2025.2477024","DOIUrl":"10.1080/19768354.2025.2477024","url":null,"abstract":"Epigenetics and epigenomics are captivating fields of molecular biology, dedicated to the exploration of heritable alterations in gene expression and cellular phenotypes, which transpire devoid of any discernible modifications to the fundamental DNA sequence. This intricate regulatory apparatus encompasses multiple mechanisms, prominently featuring DNA methylation, histone modifications, and the involvement of non-coding RNA molecules in pivotal roles. To achieve a comprehensive grasp of these diverse mechanisms, it is imperative to conduct research employing animal models as proxies for human studies. Since experimental animal models like mice and rats struggle to replicate the diverse environmental conditions experienced by humans, this review focuses on comparing common epigenetic alterations in naturally occurring tumors in canine models, which share the human environment, with those in humans. Through this, we emphasize the importance of an epigenetic regulation in the comparative medical approach to a deeper understanding of cancers and further development of cancer treatments. Additionally, we elucidate epigenetic modifications pertinent to specific developmental stages, the ageing process, and the progression of various diseases.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"12-30"},"PeriodicalIF":2.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Germline expression of Imp-L2 in Drosophila females enhances reproductive activity and longevity. 雌性果蝇种系表达Imp-L2可提高生殖活性和寿命。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2025-03-17 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2480150

Sujin Noh, Sungjoon Na, Xinge Song, Seogang Hyun

{"title":"Germline expression of Imp-L2 in Drosophila females enhances reproductive activity and longevity.","authors":"Sujin Noh, Sungjoon Na, Xinge Song, Seogang Hyun","doi":"10.1080/19768354.2025.2480150","DOIUrl":"10.1080/19768354.2025.2480150","url":null,"abstract":"The Imaginal morphogenesis protein-Late 2 (Imp-L2) in Drosophila is recognized as a functional homolog of the insulin-like growth factor (IGF) binding protein family. In this study, we report that Imp-L2 expression in germline cells during oogenesis simultaneously enhances both fecundity and lifespan in female Drosophila. Loss of Imp-L2, either through knockout or germline-specific knockdown, resulted in decreased reproductive activity, as evidenced by reduced ovary size and fecundity, along with a higher proportion of infertile flies. Conversely, overexpression of Imp-L2 specifically in germline cells enhanced reproductive activity. Imp-L2 appears to regulate germline stem cell proliferation and differentiation independently of IGF signaling. Interestingly, germline-specific knockdown of Imp-L2 shortened the lifespan of female flies, whereas its overexpression extended it. Thus, Imp-L2 expression in the germline promotes both reproductive activity and longevity, presenting an exception to the typical trade-off between reproduction and lifespan.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"31-40"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11915738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRAP1 functions in the morphogenesis of the embryonic kidney. TRAP1在胚胎肾的形态发生中起作用。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2025-03-12 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2477789

Ha Eun Kim, Taejoon Kwon, Hyo Jung Sim, Tae Joo Park

{"title":"TRAP1 functions in the morphogenesis of the embryonic kidney.","authors":"Ha Eun Kim, Taejoon Kwon, Hyo Jung Sim, Tae Joo Park","doi":"10.1080/19768354.2025.2477789","DOIUrl":"10.1080/19768354.2025.2477789","url":null,"abstract":"TNF receptor-associated protein1 (TRAP1) is a mitochondrial molecular chaperon with high homology with a cytosolic chaperon HSP90. It has been shown that TRAP1 functions as an inhibitor for apoptosis by preventing cytochrome-c release from mitochondria. In addition, TRAP1 seems to play critical roles in metabolic processes for energy production, such as glycolysis and β-oxidation. It has also been reported that TRAP1 is a direct target of PTEN-induced kinase 1 (PINK1) and may be a cause of Parkinson's disease (PD) in humans. Although the biochemical functions of TRAP1 are under intense study for the physiology and treatment of various cancers, its roles in vertebrate development have not been reported. This study shows that Xenopus TRAP1 is strongly expressed in the developing muscle, kidney, and brain tissues. Perturbation of TRAP1 function by treating TRAP1 inhibiter GTPP or microinjection of antisense-morpholino oligo (MO) caused mild defects in striated muscle fiber formation. Furthermore, the looping patterns of developing kidney tubules were perturbed, indicating that TRAP1 function is necessary for proper kidney development.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"9-18"},"PeriodicalIF":2.5,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of dasatinib and quercetin promotes osteogenic differentiation and stemness maintenance of hPDLSCs via YAP/TAZ. 达沙替尼与槲皮素联用可通过YAP/TAZ促进hPDLSCs的成骨分化和干性维持。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2025-03-12 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2477050

Yunge Qiu, Yajun Zhao, Linglu Jia, Han Xiao, Shaoqing Sun, Weiting Gu, Yong Wen

{"title":"Combination of dasatinib and quercetin promotes osteogenic differentiation and stemness maintenance of hPDLSCs via YAP/TAZ.","authors":"Yunge Qiu, Yajun Zhao, Linglu Jia, Han Xiao, Shaoqing Sun, Weiting Gu, Yong Wen","doi":"10.1080/19768354.2025.2477050","DOIUrl":"10.1080/19768354.2025.2477050","url":null,"abstract":"Human periodontal ligament stem cells (hPDLSCs) are candidate seed cells for periodontal tissue regeneration. Enhancing the stemness maintenance and osteogenic differentiation potential of hPDLSCs is conducive to their role in periodontal tissue regeneration. The combination of dasatinib and quercetin, a type of senolytic, has been reported to affect cell senescence. However, whether it can regulate the osteogenic differentiation and stemness maintenance of hPDLSCs, and the related mechanisms, remain unknown. The present study analyzed the optimal concentrations of dasatinib and quercetin in combination for hPDLSCs and found that the combination of dasatinib and quercetin enhanced osteogenic differentiation and promoted the expression of stemness-related markers in hPDLSCs. The expression levels of TAZ and YAP were improved when hPDLSCs were incubated with dasatinib and quercetin. However, the osteogenesis-promoting effects of dasatinib plus quercetin were partly attenuated when TAZ was knocked down, and their effects on stemness-related markers were suppressed when YAP was inhibited. Taken together, the combination of dasatinib and quercetin promotes the osteogenic differentiation and stemness maintenance of hPDLSCs, and YAP/TAZ may be involved in this process. This combination may hold promise for improving hPDLSCs function in periodontal tissue regeneration.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"19-29"},"PeriodicalIF":2.5,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0