Animal Cells and Systems最新文献

{"title":"Identifying candidate genes associated with hippocampal dysfunction in a hemiparkinsonian rat model by transcriptomic profiling.","authors":"Bohye Kim, Sungmoo Hong, Jeongmin Lee, Sohi Kang, Joong-Sun Kim, Chaeyong Jung, Taekyun Shin, BuHyun Youn, Changjong Moon","doi":"10.1080/19768354.2024.2348671","DOIUrl":"https://doi.org/10.1080/19768354.2024.2348671","url":null,"abstract":"<p><p>Parkinson's disease (PD) often results in hippocampal dysfunction, which leads to cognitive and emotional challenges and synaptic irregularities. This study attempted to assess behavioral anomalies and identify differentially expressed genes (DEGs) within the hippocampus of a hemiparkinsonian rat model to potentially uncover novel genetic candidates linked to hippocampal dysfunction. Striatal 6-hydroxydopamine (6-OHDA) infusions were performed unilaterally in the brains of adult SD rats, while dopaminergic impairments were verified in rats with 6-OHDA-lesioned striata. RNA sequencing and gene expression analysis unveiled 1018 DEGs in the ipsilateral rat hippocampus following 6-OHDA infusion: 631 genes exhibited upregulation, while 387 genes were downregulated (with FDR-adjusted <i>p</i>-value < 0.05 and absolute fold-change > 1.5). Gene ontology analysis of DEGs indicated that alterations in the hippocampi of 6-OHDA-lesioned rats were primarily associated with synaptic signaling, axon development, behavior, postsynaptic membrane, synaptic membrane, neurotransmitter receptor activity, and peptide receptor activity. The Kyoto Encyclopedia of Genes and Genomes analysis of DEGs demonstrated significant enrichment of the neuroactive ligand-receptor interaction, calcium signaling pathway, cAMP signaling pathway, axon guidance, and notch signaling pathway in rat hippocampi that had been subjected to striatal 6-OHDA infusion. STRING analysis confirmed a notable upregulation of eight hub genes (<i>Notch3</i>, <i>Gng4</i>, <i>Itga3</i>, <i>Grin2d</i>, <i>Hgf</i>, <i>Fgf11</i>, <i>Htr3a</i>, and <i>Col6a2</i>), along with a significant downregulation of two hub genes (<i>Itga11</i> and <i>Plp1</i>), as validated by reverse transcription-quantitative polymerase chain reaction. This study provides a comprehensive transcriptomic profile of the hippocampi in a hemiparkinsonian rat model, thereby offering insights into the signaling pathways underlying hippocampal dysfunction.</p>","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"28 1","pages":"198-215"},"PeriodicalIF":2.9,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11062273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-29 and MiR-140 regulate TRAIL-induced drug tolerance in lung cancer","authors":"Suyeon Kim, Ki Wook Lee, Yongjin Yoo, Sang Hee Park, Ji Won Lee, Suhyun Jeon, Shaginyan Illia, Pooja Joshi, Hyun Woo Park, Han-En Lo, Jimin Seo, Yeonwoo Kim, Min Chang, Tae Jin Lee, Jong Bae Seo, Sung-Hak Kim, Carlo M. Croce, Inki Kim, Sung-Suk Suh, Young-Jun Jeon","doi":"10.1080/19768354.2024.2345644","DOIUrl":"https://doi.org/10.1080/19768354.2024.2345644","url":null,"abstract":"Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has chemotherapeutic potential as a regulator of an extrinsic apoptotic ligand, but its effect as a drug is limited by innate and acq...","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"27 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ca2+ homeostasis and male fertility: a target for a new male contraceptive system","authors":"Kyung-Ha Lee, Jae Yeon Hwang","doi":"10.1080/19768354.2024.2345647","DOIUrl":"https://doi.org/10.1080/19768354.2024.2345647","url":null,"abstract":"Ca2+ is a key secondary messenger that determines sperm motility patterns. Mammalian sperm undergo capacitation, a process to acquire fertilizing ability, in the female reproductive tract. Capacita...","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"36 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140810074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sonic vibration ameliorates inflammatory diseases via the up-regulation of IL-10","authors":"Huijeong Ahn, Eui-Man Jung, Min-Woo Cho, Meoung-Geun Shin, Jae-Yeong Choi, Geun-Shik Lee","doi":"10.1080/19768354.2024.2346598","DOIUrl":"https://doi.org/10.1080/19768354.2024.2346598","url":null,"abstract":"Sonic vibration (SV), or vibroacoustic therapy, is applied to enhance local and systemic blood circulation and alleviate pain using low-frequency sine wave vibrations. However, there is limited sci...","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"26 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140842353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginkgo biloba extract ameliorates skin fibrosis in a bleomycin-induced mouse model of systemic sclerosis","authors":"Beomgu Lee, Jong Seong Roh, Hoim Jeong, Yerin Kim, Jihyeon Lee, Changun Yun, Jiyoung Park, Da-sol Kim, Jungsoo Lee, Min Wook So, Aran Kim, Dong Hyun Sohn, Seung-Geun Lee","doi":"10.1080/19768354.2024.2337761","DOIUrl":"https://doi.org/10.1080/19768354.2024.2337761","url":null,"abstract":"Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by skin and internal organ fibrosis and obliterative vasculopathy. Few effective treatments are currently available for fibros...","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"9 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140625788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of aerobic exercise and particulate matter exposure duration on the diversity of gut microbiota","authors":"Saba Imdad, Jin-Hee Kim, Byunghun So, Junho Jang, Jinhan Park, Wonchung Lim, Yoon-Kwang Lee, Woo Shik Shin, Trae Hillyer, Chounghun Kang","doi":"10.1080/19768354.2024.2338855","DOIUrl":"https://doi.org/10.1080/19768354.2024.2338855","url":null,"abstract":"Inhalation of ambient particulate matter (PM) can disrupt the gut microbiome, while exercise independently influences the gut microbiome by promoting beneficial bacteria. In this study, we analyzed...","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"93 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140579544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reprogramming of tumor-associated macrophages by metabolites generated from tumor microenvironment","authors":"Seung Woo Kim, Chan Woo Kim, Young-Ah Moon, Hong Seok Kim","doi":"10.1080/19768354.2024.2336249","DOIUrl":"https://doi.org/10.1080/19768354.2024.2336249","url":null,"abstract":"The tumor microenvironment comprises both tumor and non-tumor stromal cells, including tumor-associated macrophages (TAMs), endothelial cells, and carcinoma-associated fibroblasts. TAMs, major comp...","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"49 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140586989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tortoise-shell glue ameliorates male infertility through the hypothalamic-pituitary-gonadal axis in obese rats","authors":"Wen Sheng, Yingqiu Li, Lumei Liu, Bonan Li, Tiansong Sun, Congxu Zhu, Qinghu He","doi":"10.1080/19768354.2024.2329684","DOIUrl":"https://doi.org/10.1080/19768354.2024.2329684","url":null,"abstract":"Male infertility is a worldwide population health concern, but its etiology remains poorly understood. Tortoise-shell glue (TSG) is widely utilized as a pharmaceutical or dietary supplement for var...","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"46 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140146563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of <i>MEIS1</i> TALE homeodomain transcription factor knockdown on glioma stem cell growth.","authors":"Hyun-Jin Kim, Don Carlo Batara, Young-Jun Jeon, Seongsoo Lee, Samuel Beck, Sung-Hak Kim","doi":"10.1080/19768354.2024.2327340","DOIUrl":"10.1080/19768354.2024.2327340","url":null,"abstract":"<p><p>Myeloid ecotropic virus insertion site 1 (<i>MEIS1</i>) is a HOX co-factor necessary for organ development and normal hematopoiesis. Recently, <i>MEIS1</i> has been linked to the development and progression of various cancers. However, its role in gliomagenesis particularly on glioma stem cells (GSCs) remains unclear. Here, we demonstrate that <i>MEIS1</i> is highly upregulated in GSCs compared to normal, and glioma cells and to its differentiated counterparts. Inhibition of <i>MEIS1</i> expression by shRNA significantly reduced GSC growth in both <i>in vitro</i> and <i>in vivo</i> experiments. On the other hand, integrated transcriptomics analyses of glioma datasets revealed that <i>MEIS1</i> expression is correlated to cell cycle-related genes. Clinical data analysis revealed that <i>MEIS1</i> expression is elevated in high-grade gliomas, and patients with high <i>MEIS1</i> levels have poorer overall survival outcomes. The findings suggest that <i>MEIS1</i> is a prognostic biomarker for glioma patients and a possible target for developing novel therapeutic strategies against GBM.</p>","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"28 1","pages":"93-109"},"PeriodicalIF":2.9,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10939110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140130558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors affecting the cleavage efficiency of the CRISPR-Cas9 system.","authors":"Won Jun Jung, Soo-Ji Park, Seongkwang Cha, Kyoungmi Kim","doi":"10.1080/19768354.2024.2322054","DOIUrl":"10.1080/19768354.2024.2322054","url":null,"abstract":"<p><p>The CRISPR-Cas system stands out as a promising genome editing tool due to its cost-effectiveness and time efficiency compared to other methods. This system has tremendous potential for treating various diseases, including genetic disorders and cancer, and promotes therapeutic research for a wide range of genetic diseases. Additionally, the CRISPR-Cas system simplifies the generation of animal models, offering a more accessible alternative to traditional methods. The CRISPR-Cas9 system can be used to cleave target DNA strands that need to be corrected, causing double-strand breaks (DSBs). DNA with DSBs can then be recovered by the DNA repair pathway that the CRISPR-Cas9 system uses to edit target gene sequences. High cleavage efficiency of the CRISPR-Cas9 system is thus imperative for effective gene editing. Herein, we explore several factors affecting the cleavage efficiency of the CRISPR-Cas9 system. These factors include the GC content of the protospacer-adjacent motif (PAM) proximal and distal regions, single-guide RNA (sgRNA) properties, and chromatin state. These considerations contribute to the efficiency of genome editing.</p>","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"28 1","pages":"75-83"},"PeriodicalIF":2.9,"publicationDate":"2024-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140027187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}