Analytical Chemistry Letters最新文献_第4页

A promising hydrotropic effect of Tetra n-octyl ammonium bromide (TOAB) for thermodynamic investigation of surface tension, conductivity of indole in water 四正辛基溴化铵（TOAB）对吲哚在水中的表面张力和电导率的热力学研究具有良好的亲水效应

Analytical Chemistry Letters Pub Date : 2023-07-04 DOI: 10.1080/22297928.2023.2256339

S. Vhatkar, Kumar Abhisek, Helen Treasa Mathew, Ramesh Oraon

{"title":"A promising hydrotropic effect of Tetra n-octyl ammonium bromide (TOAB) for thermodynamic investigation of surface tension, conductivity of indole in water","authors":"S. Vhatkar, Kumar Abhisek, Helen Treasa Mathew, Ramesh Oraon","doi":"10.1080/22297928.2023.2256339","DOIUrl":"https://doi.org/10.1080/22297928.2023.2256339","url":null,"abstract":"Abstract Hydrotropes are a class of compounds that improve aqueous solubility of certain organic compounds. The aqueous solubility arising from interfacial incompatibility has been a critical industrial concern, limiting various applications. Among many such hydrotropes, the purpose of this work is to study the effect of n-octyl tetra ammonium bromide (TOAB) over the organic-inorganic incompatibility of an indole water system experimentally through conductometry and stalagmometry, for the first time. The study focussed on conductometry to determine minimum hydrotrope concentration (MHC) through specific conductivity at different temperatures. The MHC is essential in ensuring the spontaneous self-assembly of organic molecules (such as indole) in water systems. In addition to this, MHC was also determined using stalagmometry at room temperature to analyse the hydrotropic effect of TOAB. Consequently, the Hydrotropy-assisted cooperative solubilization of indole in water was investigated by specific conductivity (7.64 µs/cm), surface tension (40.29 mN/m) and Gibb’s free energy at the minimum hydrotrope concentration (MHC∼ 0.95 mM) at room temperature. In conclusion, the experimental observation over the MHC is in good agreement with thermodynamic studies, suggesting the promising nature of TOAB in improving the aqueous solubility of Indole in water. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"8 1","pages":"451 - 460"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139362890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of in-situ surfactant-based solid phase extraction for determination of low concentrations of CrIII in infusions of medicinal plants 使用基于表面活性剂的原位固相萃取法测定药用植物输液中的低浓度 CrIII

Analytical Chemistry Letters Pub Date : 2023-07-04 DOI: 10.1080/22297928.2023.2256337

Alêssa Gomes Siqueira, Bruno Elias dos Santos Costa, Lincoln Lucílio Romualdo, Vanessa Nunes Alves

{"title":"Use of in-situ surfactant-based solid phase extraction for determination of low concentrations of CrIII in infusions of medicinal plants","authors":"Alêssa Gomes Siqueira, Bruno Elias dos Santos Costa, Lincoln Lucílio Romualdo, Vanessa Nunes Alves","doi":"10.1080/22297928.2023.2256337","DOIUrl":"https://doi.org/10.1080/22297928.2023.2256337","url":null,"abstract":"Abstract A new solid phase extraction procedure, known as in situ surfactant-based solid phase extraction, has been proposed to detect the presence of CrIII in infusions of medicinal plants. In this work, a small amount of cetyltrimethylammonium bromide was injected into a sample containing CrIII, which was complexed by 1-(2-pyridylazo)-2-naphthol. After shaking, a small volume of NaClO Was added to the solution as an ion-pairing agent. After preconcentration, the settled phase was dissolved in a specific volume of HCl and then analyzed by Flame Atomic Absorption Spectrometry. A Doehlert matrix was used to optimize the CrIII complex formation conditions, such as the chelating agent concentration (0.18 mmol L-1) and pH of 9.5 in the reaction medium. A 23 factorial design was used to screen the variables that influence the extraction of the complex. The concentration of ionic-pairing agent (0.26 mol L-1) and the vortex mixer agitation time (7.51 minutes) were significant and optimized using response surface methodology. The limit of detection, limit of quantification, and preconcentration factor were 1.66 μg L-1, 5.5 μg L-1 and 12, respectively. The linear range was 5.5-5000 μg L-1. The method proved accuracy by the comparison with certified reference material APS-1071 (p>0.05), and the optimized conditions were successfully applied for the determination of CrIII in samples of medicinal plant infusions. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"23 1","pages":"355 - 368"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139363351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Removal of heavy metal ions using nano-cellulose prepared from rice husk: validation by differential pulse voltammetry 利用稻壳制备的纳米纤维素去除重金属离子：差分脉冲伏安法验证

Analytical Chemistry Letters Pub Date : 2023-07-04 DOI: 10.1080/22297928.2023.2258132

P.N. Baraskar, R.A. Samant, V. L. Gurav

{"title":"Removal of heavy metal ions using nano-cellulose prepared from rice husk: validation by differential pulse voltammetry","authors":"P.N. Baraskar, R.A. Samant, V. L. Gurav","doi":"10.1080/22297928.2023.2258132","DOIUrl":"https://doi.org/10.1080/22297928.2023.2258132","url":null,"abstract":"Abstract In this work, Cellulose was extracted from rice husk and chemically modified to produce Nano-Cellulose. It was characterized by FTIR, XRD, SEM/EDS, TEM, BET surface area analyzer, Particle size analyzer and tested for the removal of metal ions i.e. Zn(II), Cd(II), As(III), Pb(II) and Sn(II) from aqueous solution. The removal method was validated by differential pulse voltammetry (DPV). An adsorption experiment was performed to investigate the effects of initial pH, adsorbent dose, contact time and initial concentration of heavy metal ions on the adsorption capacity of nanocellulose. The experimental results revealed that the removal of metal ions on the nanocellulose was a pH-dependent process with the maximum adsorption capacity at the initial solution pH of 5–6. An adsorbent dose of 8mg/mL was sufficient for effective adsorption. In addition, the kinetics and equilibrium data are well described by pseudo-second-order kinetics and the Freundlich adsorption isotherm model at 50 min contact time and 50 mg/L initial concentration. Moreover, the desorption and re-adsorption performance was also studied, and the results revealed that the Nano-cellulose still showed good adsorption performance up to eight cycles of regeneration. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"380 1","pages":"432 - 450"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139363035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality assessment and analytical quality by design-driven RP-HPLC method development for estimation of Gymnemagenin in Gymnema sylvestre 通过设计驱动的 RP-HPLC 方法开发估算 Gymnemagenin in Gymnema sylvestre 的质量评估和分析质量

Analytical Chemistry Letters Pub Date : 2023-07-04 DOI: 10.1080/22297928.2023.2266726

V. P. Gaonkar, Vinodh Kumar Mannur, Kirankumar Hullatti

{"title":"Quality assessment and analytical quality by design-driven RP-HPLC method development for estimation of Gymnemagenin in Gymnema sylvestre","authors":"V. P. Gaonkar, Vinodh Kumar Mannur, Kirankumar Hullatti","doi":"10.1080/22297928.2023.2266726","DOIUrl":"https://doi.org/10.1080/22297928.2023.2266726","url":null,"abstract":"Abstract Gymnemic acids are the group of phytocompounds from Gymnema sylvestre known to possess hypoglycemic activity. Quantitative estimation of Gymnemic acid in terms of Gymnemagenin is essential for the standardization of G. sylvestre. In the present work, methodology combining reversed-phase HPLC with an Analytical Quality by-design approach was utilized for quantitative analysis of Gymnemagenin in G. sylvestre leaves. The quality assessment was performed by evaluating various pharmacognostic parameters as per WHO guidelines. Initially, the Analytical Target Profile and Critical Quality Attributes were identified. The method was optimized using 22 full factorial designs with a concentration of orthophosphoric acid in the aqueous phase (X1) and mobile phase ratio (X2) selected as an independent variable and, tailing factor (R1) and theoretical plates (R2) chosen as dependent variables. The optimized chromatographic conditions were identified with mobile phase, Methanol and Water (0.1% OPA) in the ratio of 85:15 v/v with flow rate of 0.8 mL/min and detection wavelength of 210 nm. Further, the amount of Gymnemagenin in crude G. sylvestre leaves and its extract was found to be 5.108% and 6.23% w/w respectively. The quality evaluation parameters were found to be within the given standard limit. Finally, it can be concluded that the combination of the HPLC method with the AQbD approach resulted in a more precise and accurate method for quantification of total Gymnemic acids in terms of Gymnemagenin. The developed method involves advantages such as efficient separation, well-defined peaks, and reduced analytical time and can be successfully applied for the analysis of Gymnemagenin in different Gymnema species and their herbal products. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"132 1","pages":"475 - 486"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139363116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of gamma irradiation on medicinal plants for both qualitative and quantitative analysis of phenolic acid 伽马辐照对药用植物酚酸定性和定量分析的评估

Analytical Chemistry Letters Pub Date : 2023-07-04 DOI: 10.1080/22297928.2023.2263449

Muhammad Hidhir Khawory, Azimah Amanah, N. Salin, Mohd Ferdaues Mohd Subki, Nurul Nadieya Mohd Nasim, M. I. Noordin, H. Wahab

{"title":"Evaluation of gamma irradiation on medicinal plants for both qualitative and quantitative analysis of phenolic acid","authors":"Muhammad Hidhir Khawory, Azimah Amanah, N. Salin, Mohd Ferdaues Mohd Subki, Nurul Nadieya Mohd Nasim, M. I. Noordin, H. Wahab","doi":"10.1080/22297928.2023.2263449","DOIUrl":"https://doi.org/10.1080/22297928.2023.2263449","url":null,"abstract":"Abstract The aim of this study is to assess how gamma irradiation affects the chemical profiles of medicinal plants. Two different forms; methanol leaf extracts and dried leaves, of the three medicinal plants were investigated. Utilising FTIR, HPLC, and LCMS techniques, the qualitative measurement of gallic acid (GA) in three different medicinal plants for both plant extract and dried plant before and after the gamma radiation treatment was carried out. In the meantime, HPLC has been used to do a quantitative analysis of GA in these plants in order to show the percentage concentration of phenolic acid. For these medicinal herbs, the FTIR study revealed that both plant extract and dried plant had comparable functional groups both before and after gamma radiation therapy. The chromatogram of the HPLC profile for these medicinal plants was similar in chemical constituents before and after gamma irradiation, but the samples that were treated with gamma irradiation had a slight increase in intensity (between 0.0002-0.0009 Au). Additionally, gamma irradiation at levels of 6-12 kGy and 9-13 kGy caused the mass peak of GA to deteriorate in mass spectrometry analysis. Furthermore, the percentage concentration of GA increased significantly as the gamma radiation dose increased for both forms. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"1 1","pages":"369 - 393"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139363250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green synthesis, structural elucidation, antimicrobial, and cytotoxic activities of a Schiff base and its Pd(II) complexes 一种希夫碱及其钯（II）配合物的绿色合成、结构解析、抗菌和细胞毒活性

Analytical Chemistry Letters Pub Date : 2023-07-04 DOI: 10.1080/22297928.2023.2256333

Akshita Jain, Sarita Beniwal, Naveen Sharma, N. Fahmi

{"title":"Green synthesis, structural elucidation, antimicrobial, and cytotoxic activities of a Schiff base and its Pd(II) complexes","authors":"Akshita Jain, Sarita Beniwal, Naveen Sharma, N. Fahmi","doi":"10.1080/22297928.2023.2256333","DOIUrl":"https://doi.org/10.1080/22297928.2023.2256333","url":null,"abstract":"Abstract This paper includes the synthesis of a Schiff base ligand and its palladium(II) complexes by conventional and green microwave-assisted techniques. They were characterized by conductivity measurements, mass spectrometry, FTIR, electronic spectroscopy, 1H NMR, and X-ray powder diffraction techniques. Based on analytical and spectral data, a square planar geometry was proposed for the metal complexes. The ligand(LH) and its metal complex[Pd(L) ] were screened for antimicrobial activity against pathogenic bacterial(Escherichia coli, Staphylococcus aureus) and fungal(Aspergillus niger, Penicillium chrysogenum) strains. Ciprofloxacin and Ketoconazole were used as standard drugs for antibacterial and antifungal activities respectively. The compounds showed moderate activity. The starting material(PdCl ), ligand(LH), and the metal complex [Pd(L) ] were screened for in vitro cytotoxic activity by MTT assay against cancerous cell lines (HeLa, MCF-7). Cisplatin was used as the standard drug. The metal complex, [Pd(L) ] was also screened for cytotoxic activity against normal cell line(HEK 293) to determine the selectivity index. The values of S.I(3.70-4.58) indicated that the complex [Pd(L) ] was more sensitive towards cancerous cell lines than normal cell line and may be studied further to assess its potential as cytotoxic agent. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"16 1","pages":"416 - 431"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139362915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioanalytical method development and Validation for determination of efonidipine hydrochloride ethanolate in rat plasma by high-performance liquid chromatography: applications for pharmacokinetic study 高效液相色谱法测定大鼠血浆中盐酸乙醇依非地平的生物分析方法开发与验证：在药代动力学研究中的应用

Analytical Chemistry Letters Pub Date : 2023-07-04 DOI: 10.1080/22297928.2023.2263012

Swati Bharati, Vinod Gaikwad, Bothiraja Chellampillai

{"title":"Bioanalytical method development and Validation for determination of efonidipine hydrochloride ethanolate in rat plasma by high-performance liquid chromatography: applications for pharmacokinetic study","authors":"Swati Bharati, Vinod Gaikwad, Bothiraja Chellampillai","doi":"10.1080/22297928.2023.2263012","DOIUrl":"https://doi.org/10.1080/22297928.2023.2263012","url":null,"abstract":"Abstract Efonidipine hydrochloride ethanolate (EFE), a dihydropyridine calcium channel blocker, is used for the treatment of hypertension. To find the EFE in pre-clinical samples (rat plasma), an HPLC method was devised for the current experiment. The biological samples were prepared using the methanolic extraction procedure to eliminate solvent effects. Using a C18 column, a pH-adjusted mobile phase of methanol and water (90:10, v/v) at a flow rate of 1 mL/min, and UV detection at 252 nm were utilized for chromatographic separation. The retention time of EFE was found to be 5.2 min. The developed bioanalytical method was validated with different validation parameters. The validation parameter results are within the acceptable range of the International Council for Harmonization guidelines. The method was successfully employed to evaluate the pharmacokinetic properties in Wistar albino rats following a single oral administration of 10 mg/kg of EFE. Therefore, the simple method would make it possible to detect EFE quickly and affordably from biological material. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"2015 1","pages":"394 - 402"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139363067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial activity of new flavanone glycoside isolated from Tradescantia spathacea Sw. 肉苁蓉中新型黄酮苷的抗菌活性研究。

Analytical Chemistry Letters Pub Date : 2023-05-04 DOI: 10.1080/22297928.2023.2243267

Kesari Singh, R. Yadava, R. Yadav

{"title":"Antibacterial activity of new flavanone glycoside isolated from Tradescantia spathacea Sw.","authors":"Kesari Singh, R. Yadava, R. Yadav","doi":"10.1080/22297928.2023.2243267","DOIUrl":"https://doi.org/10.1080/22297928.2023.2243267","url":null,"abstract":"Abstract Here we are reporting a new bioactive flavanone glycoside. The compound having molecular formula C21H22O11, melting point 123-125°C, and ESI-MS m/z 451 was isolated from the methanolic extract of Tradescantia Spathacea Sw. Its characterization and structure elucidation was done with the help of various chemical degradation, color reactions, and spectral data like UV-Visible, FT-IR, electrospray ionization mass spectrometry technique, nuclear magnetic resonance spectroscopy, etc. It was recognized as 5,7,3’4’-tetrahydroxy flavanone 3’-O-D-mannopyranoside. This study was also designed to examine the efficacy of an isolated compound against various bacteria which are responsible for food spoilage and pathogens in humans. The organic flavanone glycoside of methanolic extract exhibited a promising antibacterial activity towards Escherichia coli (gram negative) MTCC 724, Bacillus subtilis MTCC 441, Staphylococcus aureus MTCC 737, Pseudomonas aerugenosa MTCC 424, and Salmonella typhimurium MTCC 3224. A new flavanone glycoside possesses potent anti-bacterial activity so it can be utilized as a potent source of antibacterial agents for food and pharmaceutical interest. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"17 1","pages":"289 - 296"},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83227373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of RP-HPLC based analytical method for determination of cariprazine hydrochloride in bulk drug and pharmaceutical dosage form using box-behnken statistical design 建立了基于反相高效液相色谱法测定原料药和制剂制剂中盐酸卡里帕嗪含量的方法

Analytical Chemistry Letters Pub Date : 2023-05-04 DOI: 10.1080/22297928.2023.2242857

Pallavi Chiprikar, V. Mastiholimath

{"title":"Development of RP-HPLC based analytical method for determination of cariprazine hydrochloride in bulk drug and pharmaceutical dosage form using box-behnken statistical design","authors":"Pallavi Chiprikar, V. Mastiholimath","doi":"10.1080/22297928.2023.2242857","DOIUrl":"https://doi.org/10.1080/22297928.2023.2242857","url":null,"abstract":"Abstract A robust as well as precise technique aimed at determining the concentration of Cariprazine in bulk drugs along with formulations using RP-HPLC has been developed. The robustness study was optimized using Box Behnken statistical design. Separation was achieved using an Agilent 1260 Infinity II HPLC with an Agilent Zorbax Bonus RP column, as well as the mobile phase contained of methanol and 0.1% trifluoroacetic acid buffer (52.5:47.5) at a flow rate of 1 ml/min. The wavelength was set at 248 nm, and the method had an overall running period of 8 minutes, with the Cariprazine peak retention time at 3.83 min. The method exhibited linearity and accuracy for concentrations ranging from 80 to 120 μg/ml, with an R2 of 0.999 and accuracy RSD of 0.2%, 0.13%, and 0.05% for 80%, 100%, and 120%, respectively. Stress stability testing under basic conditions revealed 53.32% degradation. The technique’s limit of detection (LOD) and limit of quantification (LOQ) were very low, at 44.80 ng/ml and 135.60 ng/ml, correspondingly. These findings suggest that this method may be helpful during regular quality control evaluation of Cariprazine Hydrochloride in a variety of pharmaceutical formulations. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"59 1","pages":"297 - 312"},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84291453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Forced degradation studies and development of RP-HPLC method for related substances of Tenofovir alafenamide in tablet dosage form 替诺福韦阿拉那胺片剂中有关物质的强制降解研究及RP-HPLC法的建立

Analytical Chemistry Letters Pub Date : 2023-05-04 DOI: 10.1080/22297928.2023.2242866

Binal Patel, Hitesh J Vekaria

{"title":"Forced degradation studies and development of RP-HPLC method for related substances of Tenofovir alafenamide in tablet dosage form","authors":"Binal Patel, Hitesh J Vekaria","doi":"10.1080/22297928.2023.2242866","DOIUrl":"https://doi.org/10.1080/22297928.2023.2242866","url":null,"abstract":"Abstract The development and validation of an easy and rapid stability-indicating RP-HPLC technique for Tenofovir alafenamide and its impurities. In this newly developed method, chromatographic separation of Tenofovir alafenamide and its impurities was achieved on an Inertsil ODS-3V C18 (250 mm x 4.6 mm, 5 μ) column. The impurities were extracted by a mixture of Mobile Phase A: buffer solution: Acetonitrile: Purified water (20:02:78) and Mobile Phase B: Solvent Mixture and Purified water (75:25), The injection volume was 20 μL, the column temperature was 40°C, the flow rate was 1 mL/min, and the detection was carried out at 262 nm. The retention time of Tenofovir alafenamide, PMPA, PMPA anhydrate, Phenyl PMPA and PMPA isopropyl alaninate were 56.24, 5.69, 8.71, 25.89 and 37.13 respectively. The Correlation Coefficient within the acceptance criteria is not less than 0.999. The evaluated concentrations for Tenofovir alafenamide and its impurities were in the range of 0.5–7.5 ppm. The average recovery value was in the range of 90.2–113.9%. Tenofovir alafenamide’s LOD and LOQ were determined to be 0.1 μg/mL and 0.5 μg/mL respectively. Tenofovir alafenamide solution degradation behaviour was assessed using solution stability studies. A new, accurate, and precise method has been developed, evaluated, and elevated for the detection of impurities in Tenofovir alafenamide tablet dosage form. The results obtained from the validation study established that this method is specific, reliable, precise and effective. As a result, the proposed strategy can serve as an alternate method to determine related substances in routine analysis of tablet dosage form. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"7 1","pages":"321 - 336"},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76158176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0