Analytical Chemistry Letters最新文献_第5页

Antibacterial activity of new flavanone glycoside isolated from Tradescantia spathacea Sw. 肉苁蓉中新型黄酮苷的抗菌活性研究。

Analytical Chemistry Letters Pub Date : 2023-05-04 DOI: 10.1080/22297928.2023.2243267

Kesari Singh, R. Yadava, R. Yadav

{"title":"Antibacterial activity of new flavanone glycoside isolated from Tradescantia spathacea Sw.","authors":"Kesari Singh, R. Yadava, R. Yadav","doi":"10.1080/22297928.2023.2243267","DOIUrl":"https://doi.org/10.1080/22297928.2023.2243267","url":null,"abstract":"Abstract Here we are reporting a new bioactive flavanone glycoside. The compound having molecular formula C21H22O11, melting point 123-125°C, and ESI-MS m/z 451 was isolated from the methanolic extract of Tradescantia Spathacea Sw. Its characterization and structure elucidation was done with the help of various chemical degradation, color reactions, and spectral data like UV-Visible, FT-IR, electrospray ionization mass spectrometry technique, nuclear magnetic resonance spectroscopy, etc. It was recognized as 5,7,3’4’-tetrahydroxy flavanone 3’-O-D-mannopyranoside. This study was also designed to examine the efficacy of an isolated compound against various bacteria which are responsible for food spoilage and pathogens in humans. The organic flavanone glycoside of methanolic extract exhibited a promising antibacterial activity towards Escherichia coli (gram negative) MTCC 724, Bacillus subtilis MTCC 441, Staphylococcus aureus MTCC 737, Pseudomonas aerugenosa MTCC 424, and Salmonella typhimurium MTCC 3224. A new flavanone glycoside possesses potent anti-bacterial activity so it can be utilized as a potent source of antibacterial agents for food and pharmaceutical interest. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"17 1","pages":"289 - 296"},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83227373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of RP-HPLC based analytical method for determination of cariprazine hydrochloride in bulk drug and pharmaceutical dosage form using box-behnken statistical design 建立了基于反相高效液相色谱法测定原料药和制剂制剂中盐酸卡里帕嗪含量的方法

Analytical Chemistry Letters Pub Date : 2023-05-04 DOI: 10.1080/22297928.2023.2242857

Pallavi Chiprikar, V. Mastiholimath

{"title":"Development of RP-HPLC based analytical method for determination of cariprazine hydrochloride in bulk drug and pharmaceutical dosage form using box-behnken statistical design","authors":"Pallavi Chiprikar, V. Mastiholimath","doi":"10.1080/22297928.2023.2242857","DOIUrl":"https://doi.org/10.1080/22297928.2023.2242857","url":null,"abstract":"Abstract A robust as well as precise technique aimed at determining the concentration of Cariprazine in bulk drugs along with formulations using RP-HPLC has been developed. The robustness study was optimized using Box Behnken statistical design. Separation was achieved using an Agilent 1260 Infinity II HPLC with an Agilent Zorbax Bonus RP column, as well as the mobile phase contained of methanol and 0.1% trifluoroacetic acid buffer (52.5:47.5) at a flow rate of 1 ml/min. The wavelength was set at 248 nm, and the method had an overall running period of 8 minutes, with the Cariprazine peak retention time at 3.83 min. The method exhibited linearity and accuracy for concentrations ranging from 80 to 120 μg/ml, with an R2 of 0.999 and accuracy RSD of 0.2%, 0.13%, and 0.05% for 80%, 100%, and 120%, respectively. Stress stability testing under basic conditions revealed 53.32% degradation. The technique’s limit of detection (LOD) and limit of quantification (LOQ) were very low, at 44.80 ng/ml and 135.60 ng/ml, correspondingly. These findings suggest that this method may be helpful during regular quality control evaluation of Cariprazine Hydrochloride in a variety of pharmaceutical formulations. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"59 1","pages":"297 - 312"},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84291453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Forced degradation studies and development of RP-HPLC method for related substances of Tenofovir alafenamide in tablet dosage form 替诺福韦阿拉那胺片剂中有关物质的强制降解研究及RP-HPLC法的建立

Analytical Chemistry Letters Pub Date : 2023-05-04 DOI: 10.1080/22297928.2023.2242866

Binal Patel, Hitesh J Vekaria

{"title":"Forced degradation studies and development of RP-HPLC method for related substances of Tenofovir alafenamide in tablet dosage form","authors":"Binal Patel, Hitesh J Vekaria","doi":"10.1080/22297928.2023.2242866","DOIUrl":"https://doi.org/10.1080/22297928.2023.2242866","url":null,"abstract":"Abstract The development and validation of an easy and rapid stability-indicating RP-HPLC technique for Tenofovir alafenamide and its impurities. In this newly developed method, chromatographic separation of Tenofovir alafenamide and its impurities was achieved on an Inertsil ODS-3V C18 (250 mm x 4.6 mm, 5 μ) column. The impurities were extracted by a mixture of Mobile Phase A: buffer solution: Acetonitrile: Purified water (20:02:78) and Mobile Phase B: Solvent Mixture and Purified water (75:25), The injection volume was 20 μL, the column temperature was 40°C, the flow rate was 1 mL/min, and the detection was carried out at 262 nm. The retention time of Tenofovir alafenamide, PMPA, PMPA anhydrate, Phenyl PMPA and PMPA isopropyl alaninate were 56.24, 5.69, 8.71, 25.89 and 37.13 respectively. The Correlation Coefficient within the acceptance criteria is not less than 0.999. The evaluated concentrations for Tenofovir alafenamide and its impurities were in the range of 0.5–7.5 ppm. The average recovery value was in the range of 90.2–113.9%. Tenofovir alafenamide’s LOD and LOQ were determined to be 0.1 μg/mL and 0.5 μg/mL respectively. Tenofovir alafenamide solution degradation behaviour was assessed using solution stability studies. A new, accurate, and precise method has been developed, evaluated, and elevated for the detection of impurities in Tenofovir alafenamide tablet dosage form. The results obtained from the validation study established that this method is specific, reliable, precise and effective. As a result, the proposed strategy can serve as an alternate method to determine related substances in routine analysis of tablet dosage form. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"7 1","pages":"321 - 336"},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76158176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of a chiral LC-MS method for the enantiomeric resolution of (+) and (-)-Mebeverine Hydrochloride in bulk drug by using polysaccharide-based chiral stationary phase 基于多糖的手性固定相手性LC-MS分离原料药中(+)和(-)-盐酸美贝弗林对映体方法的建立与验证

Analytical Chemistry Letters Pub Date : 2023-05-04 DOI: 10.1080/22297928.2023.2244492

B. Gowramma, S. Ramachandran, Kaviarasan Lakshmanan, R. Kalirajan, S. N. Meyyanathan, G. Swaminathan

{"title":"Development and validation of a chiral LC-MS method for the enantiomeric resolution of (+) and (-)-Mebeverine Hydrochloride in bulk drug by using polysaccharide-based chiral stationary phase","authors":"B. Gowramma, S. Ramachandran, Kaviarasan Lakshmanan, R. Kalirajan, S. N. Meyyanathan, G. Swaminathan","doi":"10.1080/22297928.2023.2244492","DOIUrl":"https://doi.org/10.1080/22297928.2023.2244492","url":null,"abstract":"Abstract The aim of this study was to develop a simple, specific, sensitive and precise LC-MS method for the determination of mebeverine enantiomers in pharmaceutical formulations and it was validated as per ICH guidelines. The chromatographic separation was achieved on Phenomenex® Lux Cellulose 1 (250 mm x 4.6 mm i.d, 5μm particle size) column using mobile phase system containing acetonitrile: 10 mM ammonium acetate (85:15) at the flow rate of 0.8 mL/min. In the spectral investigation by LC-MS, the standard solution of Mebeverine showed major peak at m/z of 430.70, which was assigned to the [M+H] ion of Mebeverine. The described method was linear over the range of 15–75 ng /mL for (±) Mebeverine enantiomers with a correlation coefficient (r2 = 0.999). Detection limits and quantification limits of (+) Mebeverine and (-) Mebeverine were found to be 5 ng/mL and 15 ng/mL respectively. The recovery study of mebeverine from tablet formulation was found to be 99.16%. Mebeverine standard solution and mobile phase were found to be stable for at least 48h. The Mebeverine enantiomers were well resolved with mean retention times of about 1.16 min and 1.20 min respectively. The developed method was extensively validated and proved to be robust, accurate, precise, and suitable for analysis of Mebeverine enantiomers in tablet dosage form. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"5 1","pages":"313 - 320"},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75031554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of bioactive natural products from Rumex dentatus and their antimicrobial evaluation: A comparative study against various pathogenic bacteria 牙狸生物活性天然产物的分离及其抑菌活性评价:对多种致病菌的比较研究

Analytical Chemistry Letters Pub Date : 2023-05-04 DOI: 10.1080/22297928.2023.2232786

Tahirah Khaliq, Sadaqat Farooq, M. Waseem, P. Sultan, Sabiyah Akhter, Q. P. Hassan

{"title":"Isolation of bioactive natural products from Rumex dentatus and their antimicrobial evaluation: A comparative study against various pathogenic bacteria","authors":"Tahirah Khaliq, Sadaqat Farooq, M. Waseem, P. Sultan, Sabiyah Akhter, Q. P. Hassan","doi":"10.1080/22297928.2023.2232786","DOIUrl":"https://doi.org/10.1080/22297928.2023.2232786","url":null,"abstract":"Abstract Rumex dentatus is traditionally used to treat different ailments including bacterial and fungal infections. The presented study disclosed isolation of five bioactive molecules from Rumex dentatus. All the isolates were evaluated for antimicrobial activities. In this protocol the comparative study has been made on various extracts and isolated compounds in order to identify the lead molecule. Thus the activity carried, resulted, compound X2 with strong anti-microbial potential and exhibiting IC50 value between 14–32 μM. While rest of isolates showed moderate antibacterial potential. Further, the investigation of antimicrobial activity on various plant extracts against six human pathogens (five bacteria and a fungal pathogen), most of the extracts showed strong to moderate antimicrobial activity with IC50 ranging from 24–35 μM. Out of all the extracts which were evaluated for antimicrobial activities, the hexane and methanol: DCM (1:1) extracts were found to have good antibacterial potential in microtiter plate assay. The IC50 value of compound X2 was found to be favorable and presented a strong antimicrobial potential. Thus the results inferred that the plant has strong antibacterial value and hence can be used in medication for the treatment of various bacterial infections. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"52 5 1","pages":"226 - 233"},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77784290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

QbD based RP-HPLC method for simultaneous determination of a emtricitabine, tenofovir diproxil fumarate and efavirenz in tablet dosage form-an application to stability indicating assay 基于QbD的反相高效液相色谱法同时测定恩曲他滨、富马酸替诺福韦和依非韦伦片剂的含量——在稳定性指示试验中的应用

Analytical Chemistry Letters Pub Date : 2023-05-04 DOI: 10.1080/22297928.2023.2243279

Bhagavan Rajesh Babu Koppisetty, Hemant Kumar Tatapudi, Vasudha Dadi, Puthi Renuka Gayathri, P.N.Varalakshmi.K D. Komali, G. N. Challa, Varaprasada Rao Kollabathula, S. R. Yarraguntla

{"title":"QbD based RP-HPLC method for simultaneous determination of a emtricitabine, tenofovir diproxil fumarate and efavirenz in tablet dosage form-an application to stability indicating assay","authors":"Bhagavan Rajesh Babu Koppisetty, Hemant Kumar Tatapudi, Vasudha Dadi, Puthi Renuka Gayathri, P.N.Varalakshmi.K D. Komali, G. N. Challa, Varaprasada Rao Kollabathula, S. R. Yarraguntla","doi":"10.1080/22297928.2023.2243279","DOIUrl":"https://doi.org/10.1080/22297928.2023.2243279","url":null,"abstract":"Abstract The current studies entail Quality by Design (QbD)-enabled development of a simple, rapid, sensitive and cost-effective high-performance liquid chromatographic method for estimation of emtricitabine (EMT), tenofovir diproxil fumarate (TEN), and efavirenz (EFA) in combined dosage form. Systematic optimization was performed employing Box-Behnken design by selecting the flow rate, buffer molarity, and acetonitrile volume as the critical method parameters (CMPs) identified from screening studies, thus evaluating the critical analytical attributes (CAAs), namely, retention duration, theoretical plates, resolution, and asymmetry factor as the parameters of method robustness. The optimal chromatographic separation was achieved using acetonitrile and phosphate buffer (pH 3.0) 74.1:25.9 v/v as the mobile phase with a flow rate 0.91 mL/min, and UV detection at 260 nm. The method was validated as per the ICH recommended conditions, which revealed high degree of linearity, accuracy, precision, sensitivity and robustness over the existing liquid chromatographic methods of the drug. Studies on forced degradation under acid, basic, oxidative, photolytic, and thermal conditions revealed the drug’s well-resolved peak and the degradation products’ peaks as well. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"1 1","pages":"267 - 288"},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89771019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total phenolic content and antioxidant activity of date palm (Phoenix dactylifera L.) seed extracts using soxhlet method 索氏法测定枣椰树种子提取物的总酚含量及抗氧化活性

Analytical Chemistry Letters Pub Date : 2023-05-04 DOI: 10.1080/22297928.2023.2242862

Sahena Ferdosh, Kashif Ghafoor, Md. Zaidul Islam Sarker

{"title":"Total phenolic content and antioxidant activity of date palm (<i>Phoenix dactylifera</i> L.) seed extracts using soxhlet method","authors":"Sahena Ferdosh, Kashif Ghafoor, Md. Zaidul Islam Sarker","doi":"10.1080/22297928.2023.2242862","DOIUrl":"https://doi.org/10.1080/22297928.2023.2242862","url":null,"abstract":"The date palm (Phoenix dactylifera L.) is one of the oldest fruit crops grown in the arid regions of the Arabian Peninsula, North Africa, and the Middle East. It has numerous uses in medicine, pharmacognosy, and even religion, making it more than just a valuable food source. The aim of the study is to assess the yield percentage, total phenolic content (TPC), phenolic compositions, and antioxidant activity of three P. dactylifera cultivars, namely, Khudori, Sabaka, and Nabtat Ali, using methanol and hexane as solvents for Soxhlet extraction. The antioxidant potential of the extract was evaluated based on total phenolic content, the 2,2, diphenyl-2-picryl-hydroxyl (DPPH) assay, and the ferric-reducing antioxidant power (FRAP) assay, whereas phenolic compounds were identified and quantified using HPLC. Methanolic extracts of various seed cultivars showed higher yield recovery and TPC than hexane extracts. Khudori seeds demonstrated the highest antioxidant capacities compared to the other cultivars, with 124.54 ± 2.89 mg GAE /100 g for TPC, 79.67 ± 4.53 percentage inhibition for DPPH, and 1.49 ± 0.53 mMTE/100g for FRAP. It shows the potential of the Khudori seed extracts as a good source of antioxidant nutraceutical food and useful drug development that can help with free radical-related diseases.","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136375138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of a simple stability-indicating HPLC method for the quantitation of berberine in pharmaceuticals, ayurvedic, homeopathic products and novel nanoformulation 建立一种简便、稳定指示的高效液相色谱法，用于药物、阿育吠陀、顺势疗法产品和新型纳米制剂中小檗碱的定量

Analytical Chemistry Letters Pub Date : 2023-05-04 DOI: 10.1080/22297928.2023.2193588

Moazzim Soudagar, Bhaskar Kurangi, H. B. Chethan Kumar, S. Chimagave, Umesh Patil, Shruti Kolambkar

{"title":"Development and validation of a simple stability-indicating HPLC method for the quantitation of berberine in pharmaceuticals, ayurvedic, homeopathic products and novel nanoformulation","authors":"Moazzim Soudagar, Bhaskar Kurangi, H. B. Chethan Kumar, S. Chimagave, Umesh Patil, Shruti Kolambkar","doi":"10.1080/22297928.2023.2193588","DOIUrl":"https://doi.org/10.1080/22297928.2023.2193588","url":null,"abstract":"Abstract Berberine has been shown to have therapeutic advantages in the management of different diseases. Due to its actions and therapeutic applications, in compliance with “The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use”. (ICH) recommendations; a quick, precise and specific HPLC method was developed and validated. The chromatographic separation was carried out with Column C18 (4.6 mm X 250 mm), (5 microns) using 0.1% orthophosphoric acid: acetonitrile (60:40) as mobile phase, with a flow rate of 1 ml/min and at a wavelength of 349 nm. The developed method was also used for the estimation of berberine in various commercial preparations and also with formulated berberine-loaded cubosomal nano formulation according to ICH guidelines. Furthermore, forced degradation studies of berberine were investigated. The novel analytical technique was identified to be linear over the concentration ranging from 2 μg/ml to 14 μg/ml with R2 > 0.997. The method developed was accurate (with % recovery of 98.24% to 99.31%), precise (%RSD < 2.0%), and robust. The limit of quantification and limit of detection for berberine was found to be 0.064 μg/ml and 0.021 μg/ml respectively. In the presence of degradation products, the drug peak was obtained. The peaks for berberine were detected in the marketed formulations containing berberine and hence, the developed method could be applied to the estimation of berberine in berberine-containing formulations. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"53 1","pages":"244 - 256"},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74761854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emulsification-assisted spectroscopic analysis of black seed oil in alginate beads: method development and validation 海藻酸珠中黑籽油的乳化辅助光谱分析:方法开发与验证

Analytical Chemistry Letters Pub Date : 2023-05-04 DOI: 10.1080/22297928.2023.2243276

Hamzeh Alkhatib, F. Mohamed, Awis Sukarni Mohmad Sabere, Hazem Choukaife, A. Doolaanea

{"title":"Emulsification-assisted spectroscopic analysis of black seed oil in alginate beads: method development and validation","authors":"Hamzeh Alkhatib, F. Mohamed, Awis Sukarni Mohmad Sabere, Hazem Choukaife, A. Doolaanea","doi":"10.1080/22297928.2023.2243276","DOIUrl":"https://doi.org/10.1080/22297928.2023.2243276","url":null,"abstract":"Abstract This study aimed to develop and validate an ultrasonication-aided UV-Vis spectroscopy method for quantifying black seed oil (BSO) in alginate beads. The method utilized sonication at 10% power rate for 120 seconds to stabilize the emulsion droplet size of 10 mL of the emulsion diluents derived from breaking BSO-alginate beads in phosphate buffer. Absorbance measurements were taken at 400 nm, which was found to be the optimal wavelength for measuring the absorbance of turbid emulsion diluents. The method showed excellent linearity between 0.8 - 5 mg/mL (R2 = 0.9977), with Limit of Detection (LOD) and Limit of Quantification (LOQ) values of 0.082 and 0.249 mg/mL, respectively. The method also exhibited good accuracy and precision, with recovery percentages ranging from 98.02 - 101.9% (RSD = 0.15 - 1.24%) and intra-day/inter-day precision with RSD less than 2%. These findings suggest that the ultrasonication-aided spectroscopy method is a quick, accurate, and precise way to quantify encapsulated oils and could be useful for oils analysis during R&D, In Process Quality Control (IPQC), and Quality Control (QC) tests, as well as in other applications such as industrial manufacturing and academic research. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"117 1","pages":"234 - 243"},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86466962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Validation of a Simple Stability-Indicating HPLC Method for the Quantitation of Acyclovir in Pharma-ceuticals and Novel Nanoformulation 新型纳米制剂中阿昔洛韦的高效液相色谱定量方法的建立与验证

Analytical Chemistry Letters Pub Date : 2023-03-04 DOI: 10.1080/22297928.2023.2213262

Vishal U. Mane, Bhaskar Kurangi, Neha Bhasagi, S. Patil, Moazzim Soudagar, Umesh Patil

{"title":"Development and Validation of a Simple Stability-Indicating HPLC Method for the Quantitation of Acyclovir in Pharma-ceuticals and Novel Nanoformulation","authors":"Vishal U. Mane, Bhaskar Kurangi, Neha Bhasagi, S. Patil, Moazzim Soudagar, Umesh Patil","doi":"10.1080/22297928.2023.2213262","DOIUrl":"https://doi.org/10.1080/22297928.2023.2213262","url":null,"abstract":"Abstract Acyclovir has been shown to have therapeutic advantages in the management of different viral diseases. Owing to its tremendous and attractive therapeutic applications, a quick, precise, and specific HPLC method was developed and validated in compliance with ICH guidelines. The chromatographic separation was carried out with Column C18 (4.6mm X 250mm), (5 microns) by using 0.1% orthophosphoric acid (OPA) buffer: Methanol (55:45) as mobile phase, with a flow rate of 1 ml/min and at a wavelength of 253 nm. The novel analytical technique showed a peak of acyclovir at 2.4 min. The method was identified to be linear over the concentration ranging from 2 to 14 μg/ml. The method developed was accurate (with 98% recovery), precise, and robust. The developed method was also used for the estimation of acyclovir in various commercial formulations and acyclovir-loaded cubosomal nanoformulation. Furthermore, forced degradation studies for acyclovir were estimated under different stress conditions. In the presence of degradation products, the acyclovir peak was unaffected indicating the suitability of the developed method. Therefore, the developed method could be applied to the estimation of acyclovir in acyclovir-containing formulations. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"11 1","pages":"191 - 201"},"PeriodicalIF":0.0,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84942862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0