Isolation of bioactive natural products from Rumex dentatus and their antimicrobial evaluation: A comparative study against various pathogenic bacteria

Analytical Chemistry Letters Pub Date : 2023-05-04 DOI:10.1080/22297928.2023.2232786

Tahirah Khaliq, Sadaqat Farooq, M. Waseem, P. Sultan, Sabiyah Akhter, Q. P. Hassan

{"title":"Isolation of bioactive natural products from Rumex dentatus and their antimicrobial evaluation: A comparative study against various pathogenic bacteria","authors":"Tahirah Khaliq, Sadaqat Farooq, M. Waseem, P. Sultan, Sabiyah Akhter, Q. P. Hassan","doi":"10.1080/22297928.2023.2232786","DOIUrl":null,"url":null,"abstract":"Abstract Rumex dentatus is traditionally used to treat different ailments including bacterial and fungal infections. The presented study disclosed isolation of five bioactive molecules from Rumex dentatus. All the isolates were evaluated for antimicrobial activities. In this protocol the comparative study has been made on various extracts and isolated compounds in order to identify the lead molecule. Thus the activity carried, resulted, compound X2 with strong anti-microbial potential and exhibiting IC50 value between 14–32 μM. While rest of isolates showed moderate antibacterial potential. Further, the investigation of antimicrobial activity on various plant extracts against six human pathogens (five bacteria and a fungal pathogen), most of the extracts showed strong to moderate antimicrobial activity with IC50 ranging from 24–35 μM. Out of all the extracts which were evaluated for antimicrobial activities, the hexane and methanol: DCM (1:1) extracts were found to have good antibacterial potential in microtiter plate assay. The IC50 value of compound X2 was found to be favorable and presented a strong antimicrobial potential. Thus the results inferred that the plant has strong antibacterial value and hence can be used in medication for the treatment of various bacterial infections. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"52 5 1","pages":"226 - 233"},"PeriodicalIF":0.0000,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry Letters","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/22297928.2023.2232786","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Abstract Rumex dentatus is traditionally used to treat different ailments including bacterial and fungal infections. The presented study disclosed isolation of five bioactive molecules from Rumex dentatus. All the isolates were evaluated for antimicrobial activities. In this protocol the comparative study has been made on various extracts and isolated compounds in order to identify the lead molecule. Thus the activity carried, resulted, compound X2 with strong anti-microbial potential and exhibiting IC50 value between 14–32 μM. While rest of isolates showed moderate antibacterial potential. Further, the investigation of antimicrobial activity on various plant extracts against six human pathogens (five bacteria and a fungal pathogen), most of the extracts showed strong to moderate antimicrobial activity with IC50 ranging from 24–35 μM. Out of all the extracts which were evaluated for antimicrobial activities, the hexane and methanol: DCM (1:1) extracts were found to have good antibacterial potential in microtiter plate assay. The IC50 value of compound X2 was found to be favorable and presented a strong antimicrobial potential. Thus the results inferred that the plant has strong antibacterial value and hence can be used in medication for the treatment of various bacterial infections. GRAPHICAL ABSTRACT

查看原文本刊更多论文

牙狸生物活性天然产物的分离及其抑菌活性评价:对多种致病菌的比较研究

摘要牙乳传统上用于治疗各种疾病，包括细菌和真菌感染。本研究从牙鼠中分离得到5个生物活性分子。所有分离株均进行了抗菌活性评价。在本方案中，对各种提取物和分离化合物进行了比较研究，以确定铅分子。结果表明，化合物X2具有较强的抑菌活性，IC50值在14 ~ 32 μM之间。其余菌株抗菌潜力中等。此外，对不同植物提取物对6种人体病原菌(5种细菌和1种真菌)的抑菌活性进行了研究，结果表明，大部分植物提取物的抑菌活性在24 ~ 35 μM之间。在所有的抗菌活性评价中，正己烷和甲醇:DCM(1:1)提取物在微滴板试验中具有良好的抗菌潜力。化合物X2的IC50值较好，具有较强的抗菌潜力。由此推测，该植物具有较强的抗菌价值，可用于治疗各种细菌感染的药物。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Analytical Chemistry Letters

CiteScore

2.30

自引率

0.00%

发文量