Lívia Assis, Amanda Souza, Ingrid Regina Avanzi, Karolyne S Jorge Sousa, Giovanna E Santo, Gustavo O DO Amaral, João Pedro Mathias DE Souza, Julia Parisi, Cintia Cristina S Martignago, Daniel Araki Ribeiro, Ana Claudia Renno
{"title":"Peptides from frog skin for wound repair: a systematic review.","authors":"Lívia Assis, Amanda Souza, Ingrid Regina Avanzi, Karolyne S Jorge Sousa, Giovanna E Santo, Gustavo O DO Amaral, João Pedro Mathias DE Souza, Julia Parisi, Cintia Cristina S Martignago, Daniel Araki Ribeiro, Ana Claudia Renno","doi":"10.1590/0001-3765202520241037","DOIUrl":"https://doi.org/10.1590/0001-3765202520241037","url":null,"abstract":"<p><p>Skin wounds are common injuries, and many treatments have been proposed, especially membranes. Some studies have been exploring the potential of the skin of Amphibians as a source of peptides for wound treatment, which has been an extremely promising source of bioactives to be used, although there is a lack of effects on pre-clinical animal models, which makes it difficult to understand the results and the choice of the best protocol to use. In this context, the purpose of this study was to perform a systematic review of the literature to examine the effects of membranes manufactured with peptides from frogs on the process of healing skin wounds using in vivo experimental models. This systematic review was carried out based in the orientations of Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) utilizing PubMed, Scopus and Web of Science databases. This review confirms the significant benefits of using these peptides for injury repair and regeneration. Moreover, a faster healing and a full wound closure, with well-organized dermal and epidermal layers were found in the wounds treated peptides from frog skin.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20241037"},"PeriodicalIF":1.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lianderson F Franco, José Antônio M Fernandes, Gustavo C Tavares
{"title":"A new genus of long-legged cricket Luzarina (Orthoptera: Phalangopsidae) from the Brazilian Amazon Rainforest.","authors":"Lianderson F Franco, José Antônio M Fernandes, Gustavo C Tavares","doi":"10.1590/0001-3765202520250064","DOIUrl":"https://doi.org/10.1590/0001-3765202520250064","url":null,"abstract":"<p><p>In the neotropical region, the subtribe Luzarina is the most representative within Phalangopsini, currently with 128 valid species distributed in 49 genera (Cigliano et al. 2025). In this contribution, a new genus and species, <italic>Parapalpigera amazonica </italic>gen. et sp. nov., is described based on specimens collected in Northern Brazil, in the Amazon rainforest. The genus is closely related to <italic>Melanotes</italic> Desutter-Grandcolas, 1993 and <italic>Palpigera </italic>Hebard, 1928, but it can be distinguished by the following combination of characteristics: reduced tegmina without stridulatory apparatus; absent tympanum; very elongated and thin palps; shape of the endophallus sclerite; bifid endophallus apodeme; and shape of the female copulatory papilla.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20250064"},"PeriodicalIF":1.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thais G Carpanez, Livia Maria C Castro, Carolina R Dos Santos, Míriam Cristina S Amaral, Victor R Moreira
{"title":"Source, dynamics, and risks of microplastics and nanoplastics in agricultural groundwater systems.","authors":"Thais G Carpanez, Livia Maria C Castro, Carolina R Dos Santos, Míriam Cristina S Amaral, Victor R Moreira","doi":"10.1590/0001-3765202420241393","DOIUrl":"https://doi.org/10.1590/0001-3765202420241393","url":null,"abstract":"<p><p>Micro- and nanoplastics (MNPs) are emerging contaminants increasingly recognized for their environmental and health implications. While surface water systems have been extensively studied, the presence, behavior, and impacts of MNPs in groundwater remain underexplored, despite its critical role as water source worldwide. The findings in this review highlight that agricultural activities, particularly plastic mulches, pesticides containers, fertilizer bags, greenhouses, are major sources of MNP. Nanoplastics, predominant in groundwater due to their enhanced mobility in the vadose zone, exhibit a higher sorption capacity, facilitating the transport and accumulation of toxic substances such as hydrophobic organic pollutants and trace metals to/in groundwater. The review also discusses the hydrogeological and environmental factors influencing micro(nano)plastics distribution. In complement, synergistic and antagonistic effects arising from MNP interactions with other contaminants were reviewed, emphasizing the interactions with contaminants derived from agricultural practices (nitrate, herbicides, pesticides, and salts). Despite the generally low concentrations of MNPs in groundwater, their seasonal variability, combined with the aging of particles, underscores the need for long-term monitoring and risk assessments specific to groundwater systems. These findings reinforce the importance of research that addresses the unique challenges posed by micro- and nanoplastics in groundwater systems, ensuring their environmental protection.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97Suppl 3 Suppl 3","pages":"e20241393"},"PeriodicalIF":1.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of maceration time and fermentation technique on the phenolic compounds of Öküzgözü wines.","authors":"Hakan Dursun, Mustafa Bayram, Semra Topuz Türker","doi":"10.1590/0001-3765202520241467","DOIUrl":"https://doi.org/10.1590/0001-3765202520241467","url":null,"abstract":"<p><p>In this study, it was aimed to investigate the effects of different maceration times (5, 10, 15 days) and fermentation techniques [<italic>Saccharomyces cerevisiae</italic> inoculated (IF) and <italic>S. cerevisiae</italic> uninoculated (UF)] on the phenolic compounds of red wines produced from Öküzgözü grape variety. Wine samples were analyzed after maceration (AM) and after fermentation (AF) stages. Total phenolic compound (TPC) and total monomeric anthocyanin (TMA) values of wine at the AM stages were determined ranged between 1412.92-2083.75 mg gallic acid equivalent (GAE)/L; 68.69-98.08 mg/L, while the TPC and TMA values of wine at the AF stages were found ranged between 1496.20-2008.67 mg GAE/L; 66.46-77.32 mg/L. Depending on the maceration time, the highest TPC value was reached in 15 days, while the highest TMA value was reached in 10 days of maceration for NF and SF samples. The highest amount of phenolic compound determined in all wines was quinic acid (33.03-81.81 mg/L), following that gallic acid (3.86-24.71 mg/L) and (+)-catechin (3.94-34.35 mg/L). Vanillic acid was not detected in all wine samples taken at both stages. This study has importance in terms of determination of the fermentation technique of red vines produced using Öküzgözü grape variety and presents its potential for industrial scale production.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20241467"},"PeriodicalIF":1.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isaías Cabrini, Glenda B Porzani, Tiago Dos Santos, Maria Rangel, Andreia Leite, Marcelo B Molento, Eduardo José DE Arruda
{"title":"The toxic effect of glycerol-Cu(II) counterions on the free-living stages of Aedes aegypti.","authors":"Isaías Cabrini, Glenda B Porzani, Tiago Dos Santos, Maria Rangel, Andreia Leite, Marcelo B Molento, Eduardo José DE Arruda","doi":"10.1590/0001-3765202520240949","DOIUrl":"https://doi.org/10.1590/0001-3765202520240949","url":null,"abstract":"<p><p>New insecticidal products are needed to control mosquito populations reducing vector-borne diseases (VBD). Ovicidal control is essential to disease control because the egg bank replaces the population at each reproductive cycle in breeding grounds. The use of carriers and bioactive metals has already been proposed. In the present work, toxic glycerol mixtures with Cu(II) counterions (acetate, chloride, nitrate, and sulfate) were tested in different combinations, showing toxicity against eggs and larvae of <italic>A. aegypti</italic>. Glycerol is an efficient Cu(II) transporter to the larval gut's intracellular environment. When using the glycerol-Cu(II) acetate, chloride, and nitrate samples, the larval incubation rate was greater than 95%, and the larval mortality rate ranged from 17% to 65%. The glycerol-Cu(II) sulfate was more toxic to eggs, and the sample glycerol-Cu(II) acetate was more toxic to the larvae among the tested counterions. The toxic action of Cu(II) would occur through the rupture and disintegration of the peritrophic matrix, permeation, formation of complexes, and the induction of oxidative stress in the region of the midgut (mesentery) of larvae and eggs of <italic>A. aegypti</italic> in alkaline pH. Using counterions may prevent the outbreak of mosquito populations in endemic areas, positively impacting regions that suffer from VBD.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20240949"},"PeriodicalIF":1.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of agricultural-grade magnesium chloride as an anesthetic agent and a gonad sampling technique on white scar oysters (Crassostrea belcheri).","authors":"Parichart Ninwichian, Thidarat Khumrak, Pheerapon Chaikeeree, Nirandon Phuwan","doi":"10.1590/0001-3765202520241089","DOIUrl":"https://doi.org/10.1590/0001-3765202520241089","url":null,"abstract":"<p><p>The effects of agricultural-grade magnesium chloride (MgCl2) and gonad sampling on white scar oysters (<italic>Crassostrea belcheri</italic>) were evaluated. Oysters were anesthetized with 4 MgCl2 solutions at 4 concentrations (0, 30, 50, or 70 g L-1) for 24 h. The most effective solution was subsequently used to study the effects of gonad sampling using needles of different sizes (20G, 22G, or 24G) on <italic>C. belcheri</italic>. The results revealed no significant differences in the percentage of anesthetized oysters after exposure to 30, 50, or 70 g L-1 MgCl2 for 24 h. However, the 50 g L-1 MgCl2 solution was most effective at inducing anesthesia in <italic>C. belcheri,</italic> with 86.67±6.67% after 15 h. No significant differences in mortality rates were observed 7 days after anesthesia among the oysters immersed in 30, 50, or 70 g L-1 MgCl2. The 20G, 22G, and 24G needles enabled gonad sampling for sex determination, with no mortality in any treatment group after 15 days. Therefore, a cost-effective anesthesia protocol and gonad sampling method was established for <italic>C. belcheri</italic>. These protocols are valuable for <italic>C. belcheri</italic> broodstock management, facilitating effective breeding and other research studies requiring <italic>C. belcheri </italic>survival after tissue sampling.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20241089"},"PeriodicalIF":1.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antonio J S Rodrigues, Gabrielle M D Gomes, Raquel A F Neves, Carlos H S Caetano, Igor C Miyahira
{"title":"Population dynamics of Brachidontes darwinianus (Mytilidae) occurring in sympatry with the invasive Mytilopsis leucophaeata (Dreissenidae) in a coastal lagoon (Rio de Janeiro, Brazil).","authors":"Antonio J S Rodrigues, Gabrielle M D Gomes, Raquel A F Neves, Carlos H S Caetano, Igor C Miyahira","doi":"10.1590/0001-3765202520241535","DOIUrl":"https://doi.org/10.1590/0001-3765202520241535","url":null,"abstract":"<p><p>The native bivalve <italic>Brachidontes darwinianus</italic> occurs in sympatry with the invasive bivalve <italic>Mytilopsis leucophaeata</italic> in Rodrigo de Freitas Lagoon, an urban coastal lagoon located in the city of Rio de Janeiro, Brazil. Both species are found in hard substrates and form clusters around the lagoon. This study aimed to evaluate the population aspects, such as growth and mortality rates, of the native bivalve <italic>B. darwinianus</italic> and compare with data available for <italic>M. leucophaeata</italic> for this lagoon. Population data were analyzed and compared between bivalve species over two years: 2016-2017 and 2017-2018. In both years, the growth rate of <italic>B. darwinianus</italic> remained similar (K = 0.5), while the mortality rate showed a slight increase in the second year (Z2016-2017 = 2.25; Z2017-2018 = 2.9). The densities of <italic>M. leucophaeata</italic> were significantly higher than those of <italic>B. darwinianus</italic> regardless of the year analyzed. However, an increasing trend in the densities of the native bivalve was observed in the second year, suggesting a coexistence of native and invasive bivalves in the lagoon. Therefore, more studies are needed to understand the ecological interactions between these species, given that the invasive bivalve is established and can induce changes in the benthic community of this environment.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20241535"},"PeriodicalIF":1.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Danilo Marques, Aristônio M Teles, Marcelo Monge, Nádia Roque
{"title":"Lepidaploa bracteospinoflava: a new Asteraceae gem in the biodiversity of Atlantic Forest in Rio de Janeiro, Brazil.","authors":"Danilo Marques, Aristônio M Teles, Marcelo Monge, Nádia Roque","doi":"10.1590/0001-3765202520241085","DOIUrl":"https://doi.org/10.1590/0001-3765202520241085","url":null,"abstract":"<p><p><italic>Lepidaploa bracteospinoflava </italic>is a new species of Asteraceae from the Atlantic Forest in Rio de Janeiro, Brazil. The species was discovered in the high-altitude grasslands of Desengano State Park. It is characterized by the whitish sericeous on the abaxial leaf surface with prominent yellowish veins and involucral bracts with yellowish spines. We presented morphological comparisons among closely related species within the genus <italic>Lepidaploa</italic>, a dichotomous identification key for species in Rio de Janeiro state, illustrations, and a geographic distribution map. <italic>Lepidaploa bracteospinoflava</italic> is critically endangered due to its limited distribution, with an Area of Occupancy (AOO) of 3.000 km². The species is known from a single location, making it highly vulnerable to habitat loss and climate change. Field expeditions in Desengano State Park and surrounding areas are encouraged to locate additional populations. This discovery highlights the importance of continued botanical exploration and conservation efforts in critical biodiversity hotspots in Brazil.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20241085"},"PeriodicalIF":1.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Debora Luiza C Barreto, Charles L Cantrell, Seong J Kim, Camila R DE Carvalho, Sônia Claudia N DE Queiroz, Joanna Bajsa-Hirschel, Prabin Tamang, Stephen Oscar Duke, Luiz Henrique Rosa
{"title":"Phytotoxic and antifungal compounds for agriculture are interestingly produced by the endophytic fungus Fusarium sp. (Ascomycota) isolated from Piper sp. (Piperaceae).","authors":"Debora Luiza C Barreto, Charles L Cantrell, Seong J Kim, Camila R DE Carvalho, Sônia Claudia N DE Queiroz, Joanna Bajsa-Hirschel, Prabin Tamang, Stephen Oscar Duke, Luiz Henrique Rosa","doi":"10.1590/0001-3765202520241059","DOIUrl":"https://doi.org/10.1590/0001-3765202520241059","url":null,"abstract":"<p><p>Our study focused on the phytotoxic and antifungal activities of metabolites isolated from <italic>Fusarium</italic> sp., an endophyte associated with the medicinal plant <italic>Piper</italic> sp. Chromatographic separations of the <italic>Fusarium </italic>sp. crude extract led to the isolation of three analogous compounds: anhydrofusarubin (1), 5,10-dihydroxy-1,7-dimethoxy-3-methyl-1H-benzo[g]isochromene-6,9-dione (2), and javanicin (3). The structures of the isolated compounds were determined by high-resolution mass spectrometry (HRMS) analysis and direct comparison of 13C NMR data with that reported in the literature. The isolated compounds were evaluated for phytotoxic activity against <italic>Lactuca sativa</italic> and <italic>Agrostis stolonifera</italic>. All compounds exhibited strong phytotoxic activity on both plant species, completely inhibiting seed germination at a concentration of 1 mg mL-1. Additionally, the isolated compounds were evaluated against <italic>Lemna paucicostata</italic>, achieving a 50% growth inhibition (IC50) at concentration of 64, 28, and 31 μM for compounds 1, 2, and 3, respectively. The antifungal activity of these compounds was evaluated using a bioautography assay targeting the agricultural pathogen <italic>Colletotrichum fragariae</italic>. Among them, compound 2 demonstrated significant antifungal activity. Our results showed that tropical medicinal plants harbor an interesting endophyte that has a potential reservoir of bioactive compounds. Moreover, the structures of these compounds could serve as scaffold for the development of new pesticides.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20241059"},"PeriodicalIF":1.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Concepta McManus, Abilio Afonso Baeta Neves, Niels O S Câmara
{"title":"Policy from Scientific Publications from São Paulo Researchers: Analysis from the Overton Database.","authors":"Concepta McManus, Abilio Afonso Baeta Neves, Niels O S Câmara","doi":"10.1590/0001-3765202520240974","DOIUrl":"https://doi.org/10.1590/0001-3765202520240974","url":null,"abstract":"<p><p>Policy interventions often aim to address complex challenges (e.g, the UN Sustainable Development Goals) requiring input from various stakeholders. Funding organizations are placing a greater emphasis on the longer-term socioeconomic impacts of research and promoting scientific collaboration across these dimensions, assuming it will fuel such returns. This study examined São Paulo publications that influence public policies using the Overton database in SciVal (Elsevier). The data suggests that São Paulo research may have a significant influence on public policies in Brazil and worldwide. To capture this influence, there should be an increase in international and corporate collaborations, as well as a need for joint actions with policy bodies to increase usage. Open Access did not influence use in public policies, but publishing in top journals was fundamental. Impact increased when publications were used in policy documents. There was a significant influence on themes related to climate change and its control, with major contributors including intergovernmental agencies such as the World Bank, FAO, and the United Nations, followed by the USA, UK, and Germany. Brazil appears in 8th place. This study is based on Scopus data, and Overton itself has limitations, which are discussed in the paper and which may cause bias in the interpretations.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20240974"},"PeriodicalIF":1.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}