Anais da Academia Brasileira de Ciencias最新文献

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Isolation and identification of a potent probiotic Bacillus paramycoides strain SCS_MS2 from cheese whey-microbial fuel cell. 从干酪乳清微生物燃料电池中分离鉴定一株强效副芽孢杆菌。
IF 1.2 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2026-09-07 eCollection Date: 2026-01-01 DOI: 10.1590/0001-3765202620260046
Tun Rumman Jadid, Sazia Afrin Munni, Kamal Krishna Biswas, Narayan Roy, Jiangwei Li, Chang-Ping Yu, Subed Chandra Dev Sharma
{"title":"Isolation and identification of a potent probiotic Bacillus paramycoides strain SCS_MS2 from cheese whey-microbial fuel cell.","authors":"Tun Rumman Jadid, Sazia Afrin Munni, Kamal Krishna Biswas, Narayan Roy, Jiangwei Li, Chang-Ping Yu, Subed Chandra Dev Sharma","doi":"10.1590/0001-3765202620260046","DOIUrl":"https://doi.org/10.1590/0001-3765202620260046","url":null,"abstract":"<p><p>Due to the increasing prevalence of antibiotic-resistant pathogens, probiotics as alternatives to antibiotics are receiving growing attention from scientists and healthcare providers as a strategy to reduce the emergence of antibiotic-resistant microbes. Accordingly, this work is carried out for the isolation of a potent probiotic from a non-conventional source, cheese whey-microbial fuel cell. A bacterial strain SCS_MS2 isolated from bioanode of the cheese whey-microbial fuel cell was a Gram-positive bacterium having more than 98% 16S rRNA gene sequence similarity with Bacillus paramycoides. Therefore, the isolate was named Bacillus paramycoides strain SCS_MS2. Strain SCS_MS2 exhibited remarkable inhibitory activity against Shigella boydii, Escherichia coli, Shigella dysenteriae, and Staphylococcus aureus. The strain was able to grow at pH 2, with a survival rate of 77%, and tolerated salt concentrations of up to 6.5% NaCl. Additionally, strain SCS_MS2 showed good cell surface hydrophobicity and auto-aggregation capability, enhancing its potential for biofilm formation. The strain also showed notable antioxidant activity with low cytotoxicity. These findings indicate that Bacillus paramycoides strain SCS_MS2 has strong probiotic potential and may be applicable for improving human health as well as for use in poultry and cattle farming.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 3","pages":"e20260046"},"PeriodicalIF":1.2,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of Volatile Organic Compounds by HS-SPME/GC-MS and Descriptive Sensory Profile of Dark Chocolates from Different Cocoa Varieties. 不同可可品种黑巧克力挥发性有机物的HS-SPME/GC-MS测定及感官特征分析。
IF 1.2 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2026-09-07 eCollection Date: 2026-01-01 DOI: 10.1590/0001-3765202620250646
Calionara W B DE Melo, Manuela B Nascimento, Tamires R Ferreira, Joelaine J Santana, Ivia A Virgens, Lucimara M Martins, Carolina O DE Souza, Paulo R R Mesquita, Sérgio E Soares
{"title":"Determination of Volatile Organic Compounds by HS-SPME/GC-MS and Descriptive Sensory Profile of Dark Chocolates from Different Cocoa Varieties.","authors":"Calionara W B DE Melo, Manuela B Nascimento, Tamires R Ferreira, Joelaine J Santana, Ivia A Virgens, Lucimara M Martins, Carolina O DE Souza, Paulo R R Mesquita, Sérgio E Soares","doi":"10.1590/0001-3765202620250646","DOIUrl":"https://doi.org/10.1590/0001-3765202620250646","url":null,"abstract":"<p><p>Chocolate sensory quality is influenced by volatile organic compounds (VOCs) that vary among cocoa varieties. This study investigated the volatile and sensory profiles of dark chocolates made from nine different cocoa types. VOCs were analyzed using headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME/GC-MS). Sensory characteristics were evaluated through Quantitative Descriptive Analysis (QDA) by a trained panel of 12 tasters. The cocoa varieties studied were SR162, TSH188, CEPEC2002, BN34, PH16, PS1319, CCN51, IPIRANGA I of the Trinitario species, and PARÁ/PARAZINHO of the Forastero species. A total of 45 VOCs were identified. Among them, acetic acid (34.3-64.5%) and tetramethylpyrazine (4.7-16.9%) were the most abundant in all samples. Multivariate analysis highlighted key compounds differentiating the different chocolate samples. The results showed that the cocoa variety had a significant impact on the VOC content and the sensory profile of the chocolates. Sample SR162 contained higher levels of pyrazines, contributing to a pronounced aroma. In terms of sensory perception, chocolates made with BN34 and SR162 had more intense fruity aroma, sweetness, and fruity flavor. These findings suggest that SR162 offers a more appealing sensory profile, showing how cocoa genetics can affect the quality of dark chocolate.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 suppl 1","pages":"e20250646"},"PeriodicalIF":1.2,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of Bench Geometry for Efficient Slope Stability and Overburden Removal in Open Pit Mining. 露天开采有效边坡稳定和覆岩清除的台阶几何优化。
IF 1.2 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2026-09-07 eCollection Date: 2026-01-01 DOI: 10.1590/0001-3765202620251145
Tuğçe Öngen, Çağri Çerik, Mustafa Emre Yetkin
{"title":"Optimization of Bench Geometry for Efficient Slope Stability and Overburden Removal in Open Pit Mining.","authors":"Tuğçe Öngen, Çağri Çerik, Mustafa Emre Yetkin","doi":"10.1590/0001-3765202620251145","DOIUrl":"https://doi.org/10.1590/0001-3765202620251145","url":null,"abstract":"<p><p>Open-pit mining operations require a careful balance between maintaining slope stability and minimizing the amount of overburden to be removed. In this study, different bench geometries were examined to determine the most efficient configuration that satisfies both safety and economic considerations. Thirteen pit models were created and analyzed through finite element simulations, and the results were further evaluated using the Response Surface Methodology (RSM) based on a three-factor, three-level Box-Behnken experimental design. The analysis focused on three key parameters: bench height (H), berm width (W), and bench slope angle (β). Optimization was carried out to achieve a factor of safety (FoS) of at least 1.2 while minimizing overburden removal. The optimal design was obtained for H = 8 m, W = 8 m, and β = 75°, yielding a FoS of 1.23 and a stripping ratio of about 20.6 m³/ton. Verification through finite element modeling confirmed the reliability of the proposed model with 98.3% accuracy. The findings emphasize that integrating numerical modeling with experimental optimization techniques can effectively guide safe and economically viable open-pit designs.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 3","pages":"e20251145"},"PeriodicalIF":1.2,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
One stage anchor-based Semi-Supervised Learning for Fish Disease Detection and Classification. 基于锚定的半监督学习的鱼类疾病检测与分类。
IF 1.2 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2026-09-07 eCollection Date: 2026-01-01 DOI: 10.1590/0001-3765202620250727
Guangming He, Zhenchang Gao, Honghao Cai
{"title":"One stage anchor-based Semi-Supervised Learning for Fish Disease Detection and Classification.","authors":"Guangming He, Zhenchang Gao, Honghao Cai","doi":"10.1590/0001-3765202620250727","DOIUrl":"https://doi.org/10.1590/0001-3765202620250727","url":null,"abstract":"<p><p>To overcome the prohibitive annotation cost of monitoring diseases in intensive aquaculture, we propose SSFDD, a semi-supervised one-stage detector for fish disease. Using only 10% labeled data, it achieves 81% AP50 and 32% AP50:95 at 3.2 ms inference speed. This is enabled by three key components: (1) A dense anchor sampling strategy, combining RetinaNet's architecture with YOLOv5's anchor design, which improves detection of small lesions; (2) A confidence-guided pseudo-label filter that reduces teacher-student bias through dual thresholds; (3) An epoch adaptation mechanism that automatically adjusts training length based on label scarcity, stabilizing convergence without manual tuning. Experiments show SSFDD achieves competitive performance versus YOLOv5/v8/v11 baselines, which require fully labeled data. This approach enhances robustness while reducing annotation costs, offering a practical solution for real-world fish disease detection.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 suppl 1","pages":"e20250727"},"PeriodicalIF":1.2,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changes in fishing production in the northern Brazil. 巴西北部渔业生产的变化。
IF 1.2 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2026-09-04 eCollection Date: 2026-01-01 DOI: 10.1590/0001-3765202620251207
Ítalo Lutz, Julliany Freire, Gabriela Sarmento, Wellington Lima, Bianca Bentes
{"title":"Changes in fishing production in the northern Brazil.","authors":"Ítalo Lutz, Julliany Freire, Gabriela Sarmento, Wellington Lima, Bianca Bentes","doi":"10.1590/0001-3765202620251207","DOIUrl":"https://doi.org/10.1590/0001-3765202620251207","url":null,"abstract":"<p><p>This study analyzed temporal changes in fishery production along the northern coast of Brazil to assess evidence of fishing down marine food webs. Landings data from Pará State between 1997 and 2007 were used to evaluate variations in catch composition and mean trophic levels (MTL). Mean annual landings (t) by combined taxa peaked around 1998 and 2002, followed by a steady decline in later years. High-trophic groups, including large pelagic, demersal fish, and elasmobranchs, showed marked reductions in mean annual landings. Although no significant annual differences in MTL were detected, linear regression revealed a significant negative temporal trend, indicating a gradual shift toward species of lower trophic levels. These results suggest that fisheries in northern Brazil are undergoing a process of fishing down where high-value, top-predator species such as Cynoscion acoupa and Scomberomorus brasiliensis are being replaced by smaller, faster-growing taxa. This scenario is reinforced by decreases in catch volumes of the main target fishery resources. Despite limitations in data reliability, the consistent decline in trophic level underscores the need for improved monitoring systems, accurate species identification, and the implementation of ecosystem-based management strategies.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 suppl 1","pages":"e20251207"},"PeriodicalIF":1.2,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunological and Hepatic Responses to ChAdOx1-S/nCoV-19 Vaccination in Brazilian Academic Volunteers. 巴西学术志愿者对ChAdOx1-S/nCoV-19疫苗的免疫和肝脏反应
IF 1.2 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2026-08-28 eCollection Date: 2026-01-01 DOI: 10.1590/0001-3765202620251157
Viktória Aparecida G S Coelho, Graziele F DE Sousa, Amanda Cristina V F DA Silva, Flávia B Mury, Cintia M DE Barros
{"title":"Immunological and Hepatic Responses to ChAdOx1-S/nCoV-19 Vaccination in Brazilian Academic Volunteers.","authors":"Viktória Aparecida G S Coelho, Graziele F DE Sousa, Amanda Cristina V F DA Silva, Flávia B Mury, Cintia M DE Barros","doi":"10.1590/0001-3765202620251157","DOIUrl":"https://doi.org/10.1590/0001-3765202620251157","url":null,"abstract":"<p><p>COVID-19 is caused by the SARS-CoV-2 coronavirus. Vaccine development was essential for reducing hospitalizations and mortality during the pandemic. In Brazil, the ChAdOx1-S/nCoV-19 (AstraZeneca) vaccine was widely administered during the initial phase of the national immunization campaign. Despite a well-established safety profile, continuous surveillance of adverse events remains important. Mild adverse reactions occurred more frequently after the first dose and were generally self-limiting. The vaccine induced a strong IgG antibody response, demonstrating robust immunogenicity while producing minimal changes in liver function parameters. These findings support the favorable safety, tolerability, and immunogenic profile of the ChAdOx1-S/nCoV-19 vaccine in this population.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 3","pages":"e20251157"},"PeriodicalIF":1.2,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
How do abiotic stresses influence the seed germination and vigor of tree species from the Pampa biome? 非生物胁迫如何影响潘帕草原生物群系树种的种子萌发和活力?
IF 1.2 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2026-08-28 eCollection Date: 2026-01-01 DOI: 10.1590/0001-3765202620250073
Júlia Luiza Stahl, Jocimar C Milagre, Lucas José Mendes, Max Vinícios R DE Sousa, Jana S Noronha, Regina Pase, Matheus D Gazzola, Suelen C Aimi, Ezequiel Gasparin, Maristela M Araujo
{"title":"How do abiotic stresses influence the seed germination and vigor of tree species from the Pampa biome?","authors":"Júlia Luiza Stahl, Jocimar C Milagre, Lucas José Mendes, Max Vinícios R DE Sousa, Jana S Noronha, Regina Pase, Matheus D Gazzola, Suelen C Aimi, Ezequiel Gasparin, Maristela M Araujo","doi":"10.1590/0001-3765202620250073","DOIUrl":"https://doi.org/10.1590/0001-3765202620250073","url":null,"abstract":"<p><p>The Pampa biome is increasingly threatened by anthropogenic pressure and climate change, which intensify thermal extremes, water scarcity, and soil salinity, negatively affecting seed production and dispersal. This study evaluated the effects of thermal, water, and salinity stresses on the germination and vigor of Neltuma affinis and Vachellia caven, two ecologically and economically important Fabaceae species native to the Pampa parklands. The seeds were subjected to different temperatures (15, 25, 30, and 35 °C), polyethylene glycol (PEG) concentrations (0.0, -0.41, -0.58, and -0.79 MPa), and NaCl concentrations (0.0, -0.46, -0.92, and -1.37 MPa). Germination of N. affinis was not observed at 15 or 35 °C, whereas V. caven failed to germinate only at 15 °C. Under water stress, both species showed reduced germination and germination speed index (GSI). While V. caven demonstrated tolerance to salinity, N. affinis germination and GSI were inversely proportional to salinity. Overall, V. caven showed tolerance to thermal and saline stress, whereas N. affinis was significantly more sensitive to abiotic stresses. Given the limited distribution of N. affinis in Brazil, together with ongoing anthropogenic pressure and increasing climatic extremes, this species may be at risk of extinction due to reduced germination capacity and seed vigor.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 3","pages":"e20250073"},"PeriodicalIF":1.2,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regional Inequalities in Basic Sanitation and Public Health Impacts in Brazil's capitals. 巴西首都基本卫生和公共卫生影响方面的区域不平等。
IF 1.2 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2026-08-28 eCollection Date: 2026-01-01 DOI: 10.1590/0001-3765202620241531
Daniela P Vaiss, Livia S Freitas, Fernanda B DE Miranda, Denise S Brião, Stálin S Damasceno, Gabriella M G V DE Azevedo, Fabiana Finger-Jardim, Flavio Manoel R DA Silva Júnior
{"title":"Regional Inequalities in Basic Sanitation and Public Health Impacts in Brazil's capitals.","authors":"Daniela P Vaiss, Livia S Freitas, Fernanda B DE Miranda, Denise S Brião, Stálin S Damasceno, Gabriella M G V DE Azevedo, Fabiana Finger-Jardim, Flavio Manoel R DA Silva Júnior","doi":"10.1590/0001-3765202620241531","DOIUrl":"https://doi.org/10.1590/0001-3765202620241531","url":null,"abstract":"<p><p>Regional inequalities in basic sanitation remain a major public health challenge in Brazil. This study assessed the relationship between sanitation indicators and health outcomes related to Diseases Related to Inadequate Environmental Sanitation (DRSAI) in Brazilian capitals from 2010 to 2019. Mortality and hospitalization data were obtained from DATASUS, and water and sewerage coverage indicators from the National Sanitation Information System (SNIS). Pearson correlations and hierarchical cluster analyses were used to explore spatial patterns and associations. Pronounced regional disparities were observed. Northern and Northeastern capitals, including Macapá, Recife, and Salvador, had the highest DRSAI mortality and hospitalization rates, coinciding with the lowest sewerage coverage (<30%) and limited potable water access (<60%). In contrast, Southern and Southeastern capitals, including Curitiba, Porto Alegre, and São Paulo, showed better sanitation and health indicators. Sewage coverage was significantly and negatively correlated with hospitalization and mortality rates (p<0.05), indicating an inverse association between sanitation coverage and DRSAI outcomes. The findings highlight the central role of sanitation in reducing inequalities in DRSAI-related hospitalizations and mortality. Despite progress, universal access remains distant and requires targeted investments and integrated public policies to improve sanitation and public health outcomes in Brazil, particularly in the country's most socially vulnerable urban areas.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 suppl 1","pages":"e20241531"},"PeriodicalIF":1.2,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Alkaline lignin from Crataeva tapia leaves: in vitro antimicrobial, antibiofilm, cytotoxic evaluation and potential application in pharmaceutical tablets. 山楂叶碱性木质素:体外抗菌、抗生物膜、细胞毒性评价及在片剂中的潜在应用。
IF 1.2 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2026-08-28 eCollection Date: 2026-01-01 DOI: 10.1590/0001-3765202620240855
Lisandra S Lima, Diego S C Marques, Rejane P Neves, Maria Daniela S Buonafina-Paz, Andressa Nathally R Leal, Mary Angela A DE Souza, Maria Carmo A DE Lima, Iranildo José DA Cruz Filho
{"title":"Alkaline lignin from Crataeva tapia leaves: in vitro antimicrobial, antibiofilm, cytotoxic evaluation and potential application in pharmaceutical tablets.","authors":"Lisandra S Lima, Diego S C Marques, Rejane P Neves, Maria Daniela S Buonafina-Paz, Andressa Nathally R Leal, Mary Angela A DE Souza, Maria Carmo A DE Lima, Iranildo José DA Cruz Filho","doi":"10.1590/0001-3765202620240855","DOIUrl":"https://doi.org/10.1590/0001-3765202620240855","url":null,"abstract":"<p><p>The lignin extracted from the leaves of Crataeva tapia was investigated for its antimicrobial activity and potential use in solid pharmaceutical formulations. The leaves contained 35% cellulose, 23.4% hemicellulose, 17.3% lignin, 19.1% extractives, and 4.7% ash. The extraction yield after acid precipitation was 67.8%. Cytotoxicity assays in RAW 264.7, J774, Vero, V79, and HepG2 cells showed low toxicity (IC50 >1000 µg/mL) and no hemolytic activity (<10%). Lignin exhibited MIC values ranging from 512 to >1024 µg/mL, with no MBC or MFC detected. The best antibacterial result was observed against Staphylococcus aureus UFPEDA-709 (MIC 512 µg/mL), comparable to oxacillin. Among Candida strains, notable results were obtained for HAM11 (128 µg/mL; fluconazole 0.5 µg/mL) and HAM13 (128 µg/mL; fluconazole 4 µg/mL). No synergism was observed against bacteria, but additive and synergistic effects were detected in HAM11 and HAM13, respectively. Lignin reduced HAM13 biofilm formation by 16%. Ergosterol assays suggested its action on fungal cell walls and membranes. Tablets containing lignin showed hardness of 5.5 Kgf/cm², friability of 0.93%, and disintegration in 36.2 min, while those without lignin showed 4.6 Kgf/cm², 0.15%, and 31 min, respectively. These findings highlight C. tapia lignin as a versatile and promising biomaterial for sustainable fluconazole-based formulations and adjuvant therapies.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 suppl 1","pages":"e20240855"},"PeriodicalIF":1.2,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Vivo Assessment of Cardiotoxicity Induced by Difenoconazole and Prothioconazole in a Rodent Model. 二苯醚康唑和原硫康唑对啮齿动物模型心脏毒性的体内评价。
IF 1.2 4区 综合性期刊
Anais da Academia Brasileira de Ciencias Pub Date : 2026-08-28 eCollection Date: 2026-01-01 DOI: 10.1590/0001-3765202620250754
Jorge Lucas Teixeira-Fonseca, Polyana L DA Silva, Leisiane P Marques, Diego Jose B Orts, Michael Ramon L Conceição, Aline Naomi Ito, Danilo Roman-Campos
{"title":"In Vivo Assessment of Cardiotoxicity Induced by Difenoconazole and Prothioconazole in a Rodent Model.","authors":"Jorge Lucas Teixeira-Fonseca, Polyana L DA Silva, Leisiane P Marques, Diego Jose B Orts, Michael Ramon L Conceição, Aline Naomi Ito, Danilo Roman-Campos","doi":"10.1590/0001-3765202620250754","DOIUrl":"https://doi.org/10.1590/0001-3765202620250754","url":null,"abstract":"<p><p>The application of triazole fungicides in agriculture has raised concerns regarding their potential deleterious effects on human health. Thus, the present study aimed to evaluate the in vivo cardiotoxicity of fungicides difenoconazole (DFZ) and prothioconazole (PRZ), in Wistar rats subjected to daily administration of 1 mg/kg/day, corresponding to the non-observed adverse effect level (NOAEL), by gavage over a period of 28 consecutive days. No significant alterations were observed in the weights of the heart, right atrium, left atrium, spleen, liver, and lungs of the animals. Histopathological analyses of the myocardium revealed no evidence of cardiac hypertrophy. Electrocardiographic assessments did not indicate significant changes in P wave duration, PR and QT intervals, QRS complex, or RR interval. Furthermore, during the cardiac stress tests induced by caffeine and dobutamine, DFZ and PRZ did not increase the likelihood of developing cardiac arrhythmias, nor did they augment the infarct area as measured by TTC/Evans Blue staining. Also, serum levels of the cardiac biomarkers CK-MB and LDH were comparable to those of the control group. Collectively, these results suggest that DFZ and PRZ, at the administered dose and time frame, do not induce structural or electrical alterations in the hearts of rats.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"98 suppl 1","pages":"e20250754"},"PeriodicalIF":1.2,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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