{"title":"Trois réunions sur le thème du cancer","authors":"","doi":"10.1016/0769-2609(88)90170-6","DOIUrl":"https://doi.org/10.1016/0769-2609(88)90170-6","url":null,"abstract":"","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Page A1"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90170-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137183596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G Papierok , C Defives , A Daunizeau , P Wattré , J.-C Derieux
{"title":"Preparative electroelution of specific protein antigens from MicoplasmaPneumoniae: use in an enazyme-linked immunosorbent assay (Ellisa)","authors":"G Papierok , C Defives , A Daunizeau , P Wattré , J.-C Derieux","doi":"10.1016/0769-2609(88)90157-3","DOIUrl":"10.1016/0769-2609(88)90157-3","url":null,"abstract":"<div><p>A rapid, simple and preparative method is described for the recovery of the seven highest molecular weight proteins (HMWP) from <em>Mycoplasma pneumoniae</em> membrane. The yield of proteins obtained was approximately 90%. The method involved the separation of <em>M. pneumoniae</em> proteins by socium dodecyl sulphate polycrylamide gel electrophoresis (SDS-PAGE), followed by electroelution of HMWP.</p><p>These eluted antigens were used in an ELISA to measure IgG antibodies in sera 9 blood donors and 9 patients with <em>M. pneumoniae</em> infection.</p><p>The specificity fo <em>M. pneumoniae</em> HMWP was examined by competition ELISA and immunoblotting with different mycoplasma species encountered in teh respiratory tract.</p></div>","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Pages 589-603"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90157-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14282362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detection by molecular hybridization of pap, afa, and sea adherence systems in Escherichia coli strains associated with urinary and enteral infections","authors":"M Archambaud , P Courcoux , A Labigne-Roussel","doi":"10.1016/0769-2609(88)90156-1","DOIUrl":"10.1016/0769-2609(88)90156-1","url":null,"abstract":"<div><p>The genetic determinants resposible for the adherence of <em>Escherichia coli</em> to uroepithelial cells have been identified in recent years by genetic and molecular methods. Specific DNA probes for each of the three operons which have been cloned so far (<em>pap, afa, sfa/foc</em> operons) have been used in colony hybridization experiments to detect the presence of each of these operons in the chromosomal DNA of 443 strains of <em>E. coli</em>; 186 strains were frompatients with urinary tract infections (pyelonephritis, 106 strains; cystitis, 59; asymptomatic bacteriuria, 21) and 257 were strains from the stools of healthy subjects (61) or from patients with various enteral infections (196). <em>E.coli</em> strains harbouring the <em>pap</em> operon were found more frequently in the urine of patients with pyelonephritis (ifp < 0.001) and cystitis (p < 0.01) than in control stools. The presence of two operons (<em>pap</em>+<em>afa</em>) or (<em>pap</em>+<em>sfa/foc</em>) was only observed in uropathogenic strains. (p < 0.02).</p><p><em>Pap</em> and <em>sfa/foc</em> operons were never found in strains causing enteral infection; however, the <em>afa</em> operon was found in 7.6% of the enteropathogenic <em>E. coli</em>.</p></div>","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Pages 575-588"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90156-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14207623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mathematical modelling of bacterial growth at subinhibitory levels of aminoglycosides","authors":"S Comby , J.P Flandrois , G Carret , C Pichat","doi":"10.1016/0769-2609(88)90159-7","DOIUrl":"10.1016/0769-2609(88)90159-7","url":null,"abstract":"<div><p>The subinhibitory effect of antibiotics has often been studied without any clear theoretical framework; we have chosen to use mathematical bacterial growth modelling as a useful tool to analyse these biological states in a more rigorous manner. Since their mode of action and molecular target are relatively well known, aminoglycosides were well suited for this more sophisticated study of subinhibitory action. We have shown that two models (the Monod and the logistic models) regularly used in bacteriology, were adequate to describe these effects in a glucose-limited medium.</p><p>A change of model, according to antibiotic concentration, revealed the existence of two separate actions. At lower concentrations, inhibition affected mainly glucose use, the substrate remained limiting and growth mode did not change. As soon as the concentration exceeded a threshold, growth was totally disturbed, probably through a physiological ⪡catastrophe⪢. This threshold can be used to estimate bacterial susceptibility to these antibiotics.</p></div>","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Pages 613-629"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90159-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14207624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Nastasi, C Mammina, M.R Villafrate, M.F Massenti, G Scarlata, M Diquattro
{"title":"Multiple typing of strains of Salmonella enterica subsp. Bongori ser. 48:z35:- isolated in southern Italy","authors":"A Nastasi, C Mammina, M.R Villafrate, M.F Massenti, G Scarlata, M Diquattro","doi":"10.1016/0769-2609(88)90158-5","DOIUrl":"10.1016/0769-2609(88)90158-5","url":null,"abstract":"<div><p>In 1984–1987, 10 isolates of <em>Salmonella enterica</em> subsp. <em>bongori</em> ser. 48:<span>z</span><sub>35</sub>:-, 9 of human source, were identified at the Southern Italy Centre of Enterobacteriaceae. This serotype had never been identified in Southern Italy before 1984. The combined use of different typing methods, with particular reference to restriction enzyme fingerprinting of plasmid and chromosomal DNA, supports the hypothesis that all <em>Bongor</em> serovars derive from a single strain.</p></div>","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Pages 605-612"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90158-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13993492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}