Detection by molecular hybridization of pap, afa, and sea adherence systems in Escherichia coli strains associated with urinary and enteral infections

Annales de l'Institut Pasteur. Microbiology Pub Date : 1988-09-01 DOI:10.1016/0769-2609(88)90156-1

M Archambaud , P Courcoux , A Labigne-Roussel

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引用次数: 58

Abstract

The genetic determinants resposible for the adherence of Escherichia coli to uroepithelial cells have been identified in recent years by genetic and molecular methods. Specific DNA probes for each of the three operons which have been cloned so far (pap, afa, sfa/foc operons) have been used in colony hybridization experiments to detect the presence of each of these operons in the chromosomal DNA of 443 strains of E. coli; 186 strains were frompatients with urinary tract infections (pyelonephritis, 106 strains; cystitis, 59; asymptomatic bacteriuria, 21) and 257 were strains from the stools of healthy subjects (61) or from patients with various enteral infections (196). E.coli strains harbouring the pap operon were found more frequently in the urine of patients with pyelonephritis (ifp < 0.001) and cystitis (p < 0.01) than in control stools. The presence of two operons (pap+afa) or (pap+sfa/foc) was only observed in uropathogenic strains. (p < 0.02).

Pap and sfa/foc operons were never found in strains causing enteral infection; however, the afa operon was found in 7.6% of the enteropathogenic E. coli.

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pap, afa和sea粘附系统在与尿路和肠内感染相关的大肠杆菌菌株中的分子杂交检测

近年来，通过遗传和分子方法已经确定了大肠杆菌粘附于尿上皮细胞的遗传决定因素。目前已克隆的三种操纵子(pap、afa、sfa/foc操纵子)的特异性DNA探针已用于集落杂交实验，检测了443株大肠杆菌染色体DNA中这三种操纵子的存在;泌尿系感染186株(肾盂肾炎106株;膀胱炎,59岁;无症状菌尿21例，257例为健康人(61例)和各种肠道感染患者(196例)的粪便菌尿。携带pap操纵子的大肠杆菌菌株在肾盂肾炎患者尿液中更为常见(ifp <0.001)和膀胱炎(p <0.01)。两个操纵子(pap+afa)或(pap+sfa/foc)仅在尿路致病菌株中存在。(p & lt;0.02)。在引起肠道感染的菌株中未发现Pap和sfa/foc操作子;然而，在7.6%的致病性大肠杆菌中发现了afa操纵子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Annales de l'Institut Pasteur. Microbiology

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