A Béji , F Mégraud , P Vincent , F Gavini , D Izard , H Leclerc
{"title":"GC content of DNA of Campylobacter pylori and other species belonging or related to the genus Campylobacter","authors":"A Béji , F Mégraud , P Vincent , F Gavini , D Izard , H Leclerc","doi":"10.1016/0769-2609(88)90152-4","DOIUrl":"10.1016/0769-2609(88)90152-4","url":null,"abstract":"<div><p>DNA of type strain <em>Campylobacter pylori</em> NCTC 11637 and 32 other strains of <em>C. pylori</em> recovered from gastric biopsy specimens was examined by thermal denaturation for its guanine-plus-cytosine (GC) content.</p><p>The GC content of strain NCTC 11637 was 35.6 mol % (standard deviation (SD) 0.3), and the GC content of the 32 other <em>C. pylori</em> strains ranged from 34.1 to 37.5 mol % (average value 35.2, SD 1.0). A total of 14 type strains of other <em>Campylobacter</em> and <em>Wolinella</em> species were included in this study and the results obtained were compared with those cited in the literature.</p></div>","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Pages 527-534"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90152-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14379612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cours et travaux pratiques de microbiologie des aliments institut pasteur","authors":"","doi":"10.1016/0769-2609(88)90179-2","DOIUrl":"https://doi.org/10.1016/0769-2609(88)90179-2","url":null,"abstract":"","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Page A4"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90179-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137185617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"2es Journées internationales de biologie médicale et de biotechnologie","authors":"","doi":"10.1016/0769-2609(88)90178-0","DOIUrl":"https://doi.org/10.1016/0769-2609(88)90178-0","url":null,"abstract":"","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Page A3"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90178-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137185618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Conférence internationale sur les porteurs d'infections sanguines sur le lieu de travail","authors":"","doi":"10.1016/0769-2609(88)90175-5","DOIUrl":"https://doi.org/10.1016/0769-2609(88)90175-5","url":null,"abstract":"","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Page A3"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90175-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137185620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Influence d'une dose binhibitrice d'amphotéricine B sur la composition en acides gras et stérols cellulaires de Candida albicans","authors":"M Mpona-Minga, A Hakkou, J Coulon, R Bonaly","doi":"10.1016/0769-2609(88)90154-8","DOIUrl":"https://doi.org/10.1016/0769-2609(88)90154-8","url":null,"abstract":"<div><p>Modifications in the cellular lipids of <em>Candida albicans</em> VC32 during its growth in the presence of a subinhibitory dose of amphotericin B were studied. Intracellular accumulation of fatty acids which appeared during growth was retarded in the presence of this antifungal agent after initial stimulation of insaturated fatty acids. During the growth stage of the yeasts, sterols accumulated in cells through the action of amphotericin B. This accumulation was 80% during the growth state, but was only 13% in the stationary state. The relative level of ergosterol decreased through the action of this antifungal agent, but its precursors, namely ergosta-5,7-dien-3β-ol, episterol and fecosterol increased. The higher oxygenation of the cells due to the effect of this antifungal agent appeared to be responsible for the accumulation of unsaturated fatty acids and sterols in growing cells.</p></div>","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Pages 547-555"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90154-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91635340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diffusion veineuse et capillaire de enterococcus faecalis lors de bactériémies expérimentales chez le poulet","authors":"J.C Labarthe , J.F Guillot , A Brée , C Mouline","doi":"10.1016/0769-2609(88)90160-3","DOIUrl":"10.1016/0769-2609(88)90160-3","url":null,"abstract":"<div><p>Capillary and venous diffusion of bacteria were studied in chickens after intravenous inoculation with a strain of <em>Enterococcus faecais</em> isolated from a case of septicaemia in a human neonate.</p><p>One group of 13 chickens was inoculated with 10<sup>8</sup> bacteria/animal and another group of 20 chickens with 10<sup>7</sup> bacteria/animal. The level of bacteraemia in the first group of chickens was similar in capillary and venous blood. With 10<sup>8</sup><em>E. faecalis</em>, the clearance of bacteria was very weak. The level of bacteraemia in the second group was significantly higher (after 180 min) in capillary than in venous blood, which suggests slower bacterial clearance in capillary than in venous blood. However, clearance was more efficient with the inoculum of 10<sup>7</sup><em>E. faecalis</em> than with the first type of inoculum.</p><p>These experimental data confirm the greated sensitivity of capillary rather than venous sampling, which we have already observed in chickens, under similar experimental conditions, with <em>Escherichia coli</em> K12. Persistance of bacteria in the capillaries could account for the higher level of sensitivity of capillary microblood culture than venous blood culture, which is in agreement with the capillary trapping hypothesis of Knudson and Alden.</p></div>","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Pages 631-636"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90160-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14282363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Heat shock proteins in the moderately halophilic bacterium deleya halophila: Protective effect of high salt concentration against thermal shock","authors":"S Karamanou, P Katinakis","doi":"10.1016/0769-2609(88)90150-0","DOIUrl":"10.1016/0769-2609(88)90150-0","url":null,"abstract":"<div><p>The moderately halophilic eubacterium <em>Deleya halophila</em> grown in medium containing 1 M or 2.5 M NaCl was heat-shocked at various temperatures and the electrophoretic patterns of pulse-labelled proteins were examined. Several polypeptides were induced (heat shock proteins, or hsp) at all temperatures. However, the level of induction of some hsp was dependent on the severity of the thermal shock as well as the salt concentration of the growth medium. Time course studies revealed that synthesis of some of the hsp was transient when cells were grown in 1 M NaCl, while growth at 2.5 M NaCl resulted in the synthesis of most of the hsp at almost maximal level for at least 60 min following temperature shift-up. When cells were returned to normal growth temperature (30°C) after a heat shock treatment (47°C for 5 min), normal protein synthesis resumed faster when cells were grown in 1 M than in 2.5 M NaCl. During the recovery period, several major hsp appeared to be synthesized at near maximal level at both salt concentrations.</p></div>","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Pages 505-514"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90150-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14381206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cours international: Les nouvelles générations de vaccins destinés à l'homme et aux animaux","authors":"","doi":"10.1016/0769-2609(88)90177-9","DOIUrl":"https://doi.org/10.1016/0769-2609(88)90177-9","url":null,"abstract":"","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Page A3"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90177-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137200760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}