G Papierok , C Defives , A Daunizeau , P Wattré , J.-C Derieux
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引用次数: 4
Abstract
A rapid, simple and preparative method is described for the recovery of the seven highest molecular weight proteins (HMWP) from Mycoplasma pneumoniae membrane. The yield of proteins obtained was approximately 90%. The method involved the separation of M. pneumoniae proteins by socium dodecyl sulphate polycrylamide gel electrophoresis (SDS-PAGE), followed by electroelution of HMWP.
These eluted antigens were used in an ELISA to measure IgG antibodies in sera 9 blood donors and 9 patients with M. pneumoniae infection.
The specificity fo M. pneumoniae HMWP was examined by competition ELISA and immunoblotting with different mycoplasma species encountered in teh respiratory tract.
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