G Papierok , C Defives , A Daunizeau , P Wattré , J.-C Derieux
{"title":"制备性电洗脱肺炎微质体特异性蛋白抗原:用于酶联免疫吸附试验(elisa)","authors":"G Papierok , C Defives , A Daunizeau , P Wattré , J.-C Derieux","doi":"10.1016/0769-2609(88)90157-3","DOIUrl":null,"url":null,"abstract":"<div><p>A rapid, simple and preparative method is described for the recovery of the seven highest molecular weight proteins (HMWP) from <em>Mycoplasma pneumoniae</em> membrane. The yield of proteins obtained was approximately 90%. The method involved the separation of <em>M. pneumoniae</em> proteins by socium dodecyl sulphate polycrylamide gel electrophoresis (SDS-PAGE), followed by electroelution of HMWP.</p><p>These eluted antigens were used in an ELISA to measure IgG antibodies in sera 9 blood donors and 9 patients with <em>M. pneumoniae</em> infection.</p><p>The specificity fo <em>M. pneumoniae</em> HMWP was examined by competition ELISA and immunoblotting with different mycoplasma species encountered in teh respiratory tract.</p></div>","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 5","pages":"Pages 589-603"},"PeriodicalIF":0.0000,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90157-3","citationCount":"4","resultStr":"{\"title\":\"Preparative electroelution of specific protein antigens from MicoplasmaPneumoniae: use in an enazyme-linked immunosorbent assay (Ellisa)\",\"authors\":\"G Papierok , C Defives , A Daunizeau , P Wattré , J.-C Derieux\",\"doi\":\"10.1016/0769-2609(88)90157-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A rapid, simple and preparative method is described for the recovery of the seven highest molecular weight proteins (HMWP) from <em>Mycoplasma pneumoniae</em> membrane. The yield of proteins obtained was approximately 90%. The method involved the separation of <em>M. pneumoniae</em> proteins by socium dodecyl sulphate polycrylamide gel electrophoresis (SDS-PAGE), followed by electroelution of HMWP.</p><p>These eluted antigens were used in an ELISA to measure IgG antibodies in sera 9 blood donors and 9 patients with <em>M. pneumoniae</em> infection.</p><p>The specificity fo <em>M. pneumoniae</em> HMWP was examined by competition ELISA and immunoblotting with different mycoplasma species encountered in teh respiratory tract.</p></div>\",\"PeriodicalId\":77666,\"journal\":{\"name\":\"Annales de l'Institut Pasteur. Microbiology\",\"volume\":\"139 5\",\"pages\":\"Pages 589-603\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0769-2609(88)90157-3\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annales de l'Institut Pasteur. Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0769260988901573\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annales de l'Institut Pasteur. Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0769260988901573","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Preparative electroelution of specific protein antigens from MicoplasmaPneumoniae: use in an enazyme-linked immunosorbent assay (Ellisa)
A rapid, simple and preparative method is described for the recovery of the seven highest molecular weight proteins (HMWP) from Mycoplasma pneumoniae membrane. The yield of proteins obtained was approximately 90%. The method involved the separation of M. pneumoniae proteins by socium dodecyl sulphate polycrylamide gel electrophoresis (SDS-PAGE), followed by electroelution of HMWP.
These eluted antigens were used in an ELISA to measure IgG antibodies in sera 9 blood donors and 9 patients with M. pneumoniae infection.
The specificity fo M. pneumoniae HMWP was examined by competition ELISA and immunoblotting with different mycoplasma species encountered in teh respiratory tract.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
