Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology最新文献

Function of polymorphonuclear neutrophilic leucocytes. Comparison of leucocytes from blood and exudate in healthy volunteers. 多形核嗜中性白细胞的功能。健康志愿者血液与渗出液白细胞的比较。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1982.TB01410.X

J. Wandall

{"title":"Function of polymorphonuclear neutrophilic leucocytes. Comparison of leucocytes from blood and exudate in healthy volunteers.","authors":"J. Wandall","doi":"10.1111/J.1699-0463.1982.TB01410.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1982.TB01410.X","url":null,"abstract":"The function of polymorphonuclear neutrophils from blood (B-PMN) and from exudates (E-PMN) was studied in healthy volunteers. The E-PMNs were isolated from skin windows with chambers and the chemotactic, phagocytic and Nitro Blue Tetrazolium (NBT) reducing activity measured and compared to that of simultaneously obtained B-PMNs. The chemotactic and random migration of E-PMNs in Boyden chambers measured by the leading front and by the chemotactic index were reduced compared with B-PMNs (p less than 0.01). Serum independent phagocytosis of paraffin oil emulsions was significantly increased (p less than 0.01) by E-PMNs after 12, 24 and 48 hours and not correlated to the function of B-PMNs. Opsonization with autologous serum increased the phagocytosis by both E-PMNs and B-PMNs, but mainly the latter. The NBT reduction by E-PMNs was increased (p less than 0.01) and positively correlated to the NBT reduction by resting B-PMNs. In contrast, there was no difference in NBT reduction between phagocytosing E-PMNs and B-PMNs. Mobilization of PMNs to an inflammatory focus in healthy subjects induces marked changes in the function, and B-PMN function cannot always be assumed to reflect the function of PMNs from inflammatory sites.","PeriodicalId":77653,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","volume":"49 1","pages":"7-13"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77924444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

The heterologous immune complex glomerulonephritis. A dose dependent glomerulonephritis with acute, latent and chronic phases in a long-term study. 异源免疫复合物肾小球肾炎。剂量依赖性肾小球肾炎急性、潜伏期和慢性期的长期研究。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1982.TB01445.X

B. Iversen, R. Matre, J. Ofstad

{"title":"The heterologous immune complex glomerulonephritis. A dose dependent glomerulonephritis with acute, latent and chronic phases in a long-term study.","authors":"B. Iversen, R. Matre, J. Ofstad","doi":"10.1111/J.1699-0463.1982.TB01445.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1982.TB01445.X","url":null,"abstract":"The pathophysiology, histology and immunohistology of acute and chronic heterologous immune complex glomerulonephritis were investigated in a long-term study in male Wistar rats. The glomerulonephritis showed 3 phases: an initial nephrotic syndrome, a latent phase with stable proteinuria (40 mg/24 h), and a terminal phase with increasing proteinuria and blood pressure, and declining serum protein concentration and creatinine clearance. Antiserum doses of 1.0, 1.5 and 2.0 ml induced maximal proteinuria (112, 257 and 272 mg/24 h respectively) after 14 days whereas normal rabbit serum and 0.5 ml antiserum gave no proteinuria. After 100 days, the rats injected with 1.0 ml of antiserum did not show physiological signs of renal disease; in the rats injected with 1.5 ml of antiserum the disease run a chronic course. Equal amounts of rabbit IgG, rat IgG and rat C3 were found in 10 glomeruli from rats 100 days after injection of 0.5, 1.0 and 2.0 ml (p greater than 0.10). Intramembranous deposits and spike formation were observed in all groups. All changes increased with greater antiserum doses. Chronically diseased animals observed from 500 to 750 days showed deposits of rabbit IgG in the basement membrane, and in most animals small amounts of rat IgG and rat C3 were also observed. This is compatible with a sustained stimulus for antibody formation throughout the course of this type of glomerulonephritis.","PeriodicalId":77653,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","volume":"63 1","pages":"241-50"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74137497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The alternative complement pathway in chickens. Purification of factor B and production of a monospecific antibody against it. 鸡的替代补体途径。因子B的纯化及单特异性抗体的制备。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1986-12-01

C Koch

引用次数: 0

Autoreactive lymphocytes in thyroid disorders. II. Comparison of anti-thyroglobulin antibody production by plaque-forming cell, radio-immunological and enzyme-linked immunosorbent assays. 自身反应性淋巴细胞在甲状腺疾病中的作用。2斑块形成细胞法、放射免疫法和酶联免疫吸附法抗甲状腺球蛋白抗体产生的比较。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1986-06-01

J Petersen, U Feldt-Rasmussen, M Høier-Madsen, F Larsen, S Husby, K Siersbaek-Nielsen

{"title":"Autoreactive lymphocytes in thyroid disorders. II. Comparison of anti-thyroglobulin antibody production by plaque-forming cell, radio-immunological and enzyme-linked immunosorbent assays.","authors":"J Petersen, U Feldt-Rasmussen, M Høier-Madsen, F Larsen, S Husby, K Siersbaek-Nielsen","doi":"","DOIUrl":"","url":null,"abstract":"Blood mononuclear cells (MNC) from 9 randomly selected patients with autoimmune thyroiditis were stimulated in vitro with pokeweed mitogen (PWM), a polyclonal B lymphocyte activator. The secretion of immunoglobulins (Ig) and anti-thyroglobulin antibodies (TgAb) was assayed by means of haemolytic plaque-forming cell (PFC) assays, radio-immune assay (RIA) and enzyme-linked immunosorbent assays (ELISA). Total Ig and TgAb production was maximal using MNC cultured at 1.0 X 10(6)/ml as estimated by PFC, RIA and ELISA. The Ig and TgAb production as measured by RIA and ELISA was 1.5 - 3 times higher after 12 days' culture compared to 6 days' culture. Ig and TgAb production measured by PFC-assays at day 6 correlated positively to the results obtained by RIA and ELISA at day 12. PWM-induced TgAb secretion correlated positively to TgAb titres in serum. As judged by PFC, TgAb production was found in 8/9 patients; about 5% (range 0 - 7.9%) of the total PWM-stimulated IgG-secreting cells were involved in TgAb secretion. TgAb production as measured by ELISA and RIA was found in 6/9 patients. By reference to an affinity-purified human TgAb preparation, the TgAb secretion was about 0.7% (range 0 - 21.3%) of the total PWM-induced IgG secretion.","PeriodicalId":77653,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","volume":"94 3","pages":"113-7"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14652181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison between homogeneous phase radioassay and enzyme-linked immunosorbent assay for measurement of antithyroglobulin antibody content in serum. Relation to presence of thyroglobulin. 均相放射法与酶联免疫吸附法测定血清中抗甲状腺球蛋白含量的比较。与甲状腺球蛋白的存在有关。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1986-02-01

U Feldt-Rasmussen, M Høier-Madsen, H S Hansen, M Blichert-Toft

引用次数: 0

Augmentation of cytotoxicity by natural killer (NK) cells after adrenaline administration in man. 肾上腺素对人体NK细胞毒性的增强作用。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1984-02-01

E Tønnesen, J Tønnesen, N J Christensen

引用次数: 0

Complement factor D concentrations in normal sera: comparison of immunochemical and functional determinations. 正常血清中补体因子D浓度:免疫化学测定和功能测定的比较。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1983-12-01

G Sturfelt, L Truedsson

引用次数: 0

Nitroblue tetrazolium slide test. Use of the phorbol-myristate-acetate-stimulated NBT-reduction slide test for routine and prenatal detection of chronic granulomatous disease and diagnosis of heterozygous carriers. 硝蓝四氮唑玻片试验。利用磷酚-肉豆酸酯刺激的nbt还原玻片试验进行常规和产前慢性肉芽肿疾病的检测和杂合携带者的诊断。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1983-12-01

K S Johansen

{"title":"Nitroblue tetrazolium slide test. Use of the phorbol-myristate-acetate-stimulated NBT-reduction slide test for routine and prenatal detection of chronic granulomatous disease and diagnosis of heterozygous carriers.","authors":"K S Johansen","doi":"","DOIUrl":"","url":null,"abstract":"Chronic granulomatous disease (CGD) in utero has recently been detected by a new qualitative nitro blue tetrazolium (NBT) reduction slide test using phorbol-myristate-acetate (PMA) as a stimulus. The technique is simple and inexpensive and requires only a few microlitres of blood. Reported here is an evaluation of this method as applied to routine clinical diagnosis. The blood granulocytes from 300 normal individuals and 49 CGD patients and their relatives were tested and the results compared to the conventional in vitro function tests. In normal individuals the number of abnormal cells was very low never exceeding 2% of granulocytes. CGD patients (14 out of 15) diagnosed by conventional functional tests showed no positive cells in the NBT-PMA test. One patient diagnosed by functional tests had 4% positive cells. Thirty-four relatives of these patients were tested and eleven were found to have fewer positive cells than normal in the NBT-PMA slide test ranging from 16-88% of all granulocytes. These are presumably carriers, a finding supported by granulocyte function tests. All these individuals were female, mothers, sisters or maternal aunts of male CGD patients, thus presumably X-linked heterozygote carriers of CGD. An example of successful prenatal diagnosis using the PMA-NBT test is described. The results show that the PMA-NBT test provides a simple and reproducible method for routine diagnosis of CGD and CGD X-linked heterozygote carriers.","PeriodicalId":77653,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","volume":"91 6","pages":"349-54"},"PeriodicalIF":0.0,"publicationDate":"1983-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17727335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of temperature on polymorphonuclear leukocyte function. 温度对多形核白细胞功能的影响。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1983-12-01

K S Johansen, E M Berger, J E Repine

{"title":"Effect of temperature on polymorphonuclear leukocyte function.","authors":"K S Johansen, E M Berger, J E Repine","doi":"","DOIUrl":"","url":null,"abstract":"The effect of temperature on the function of polymorphonuclear leukocytes (PMN) has been investigated in vitro. Increases in temperature from 37 degrees C to 40 degrees C progressively increased chemiluminescence (CL) responses by PMN after stimulation by Staphylococcus aureus, zymosan or phorbol myristate acetate (PMA) while increases above 40 degrees C decreased these functions. Temperature increases from 37 degrees C to 40 degrees C also produced increased PMN bactericidal activity against S. aureus. In contrast, similar increases in temperature did not change superoxide production by PMN stimulated by PMA. Incubation of PMN at the various temperatures did not cause release of LDH indicating that damage to PMN was not the cause of reduced PMN chemiluminescence and bactericidal activity seen within the temperature range studied. The discrepancy between the influence of temperature on PMN chemiluminescence and bactericidal activity of PMN compared to superoxide anion production by PMN suggests that superoxide anion production may not be solely, or at least directly, responsible for killing of bacteria. Careful temperature control is needed when assaying PMN function. Febrile responses up to 40 degrees C may play a beneficial role in host defense.","PeriodicalId":77653,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","volume":"91 6","pages":"355-9"},"PeriodicalIF":0.0,"publicationDate":"1983-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17385476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Degranulation and enzyme release during phagocytosis of inert particles and of bacteria by polymorphonuclear neutrophil granulocytes. 多形核中性粒细胞吞噬惰性颗粒和细菌时的脱粒和酶释放。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1983-12-01

I Talstad, H Dalen, V Lehmann

{"title":"Degranulation and enzyme release during phagocytosis of inert particles and of bacteria by polymorphonuclear neutrophil granulocytes.","authors":"I Talstad, H Dalen, V Lehmann","doi":"","DOIUrl":"","url":null,"abstract":"The degranulation and release of lysosomal (myeloperoxidase, beta-glucuronidase, lysozyme) and cytoplasmic (lactate dehydrogenase-LDH) enzymes from polymorphonuclear neutrophil granulocytes (PMG) during phagocytosis of inert latex particles or bacteria were studied. Degranulation was much faster and more pronounced by phagocytosis of bacteria than of inert particles. A high frequency of lysosome-lysosome as well as lysosome-phagosome fusions suggested that granular material was transported by lysosome- lysosome- phagosome fusions. During bacterial phagocytosis there was evidence of release of granular material into cytoplasm causing enzymatic disintegration. After 60 minutes cell lysis occurred in about 5 per cent of the cells during bacterial phagocytosis. There was non-specific release of LDH during phagocytosis of inert particles, probably due to erythro-phagocytosis. After 60 minutes the release during bacterial phagocytosis amounted to 20-30 per cent of the enzyme content of the cells. A nearly equal release of lysosomal and cytoplasmic enzymes gave support for the idea that cell lysis was the main mechanism of enzyme release.","PeriodicalId":77653,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","volume":"91 6","pages":"403-11"},"PeriodicalIF":0.0,"publicationDate":"1983-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17385478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0