Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology最新文献_第7页

Effects of hyperthermia on human natural killer cells. 热疗对人体自然杀伤细胞的影响。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1983-02-01

M Onsrud

{"title":"Effects of hyperthermia on human natural killer cells.","authors":"M Onsrud","doi":"","DOIUrl":"","url":null,"abstract":"Lymphocytes from healthy blood donors were exposed to temperatures between 37 degrees C and 42 degrees C for up to three hours and then tested for natural killer (NK) activity using K562 cells as targets in a 3-h 51Cr-release assay. For a given level of hyperthermia a semilogarithmic decrease in NK activity relative to the treatment period was seen. NK effectors exposed to 42 degrees C for one hour lost 90% of their cytotoxic capacity compared to effectors kept at 37 degrees C. The depression in NK activity could Not be repaired by overnight incubation at 37 degrees C or by interferon treatment. Heating also inhibited the induction of NK-like cells during mixed lymphocyte culture (MLC), while exposure of either responder or stimulator cells to hyperthermia did not affect the degree of MLC-proliferation. The heating only slightly decreased lymphocyte viability--as determined by trypan blue exclusion--whereas a marked and permanent reduction in the number of cells bearing Fc-receptors for IgG occurred. The content of E-rosetting cells decreased initially, but was normalized after overnight incubation. The findings indicate that NK cells and T cells are differentially sensitive to in vitro hyperthermic treatment.","PeriodicalId":77653,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","volume":"91 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"1983-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17286788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Phenotypical, functional and chromosomal studies of lymphocytes from patients with infectious mononucleosis. 传染性单核细胞增多症患者淋巴细胞的表型、功能和染色体研究。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1983-02-01

J R Jensen, S Bisballe, E B Pedersen, K D Christensen, K Thestrup-Pedersen

引用次数: 0

Secretion of IgM-rheumatoid factor and IgM by blood lymphocytes in rheumatoid arthritis. 类风湿关节炎患者外周血淋巴细胞分泌IgM-类风湿因子及IgM。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1983-02-01

C Heilmann, J Petersen, V Andersen, O J Bjerrum, B Dinesen

引用次数: 0

An easy method for comparing antibody affinities to related antigens. 一种比较抗体与相关抗原亲和力的简便方法。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1983-02-01

B Aasted, B Avery

引用次数: 0

The heterologous immune complex glomerulonephritis. A dose dependent glomerulonephritis with acute, latent and chronic phases in a long-term study. 异源免疫复合物肾小球肾炎。剂量依赖性肾小球肾炎急性、潜伏期和慢性期的长期研究。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1982-10-01

B M Iversen, R Matre, J Ofstad

{"title":"The heterologous immune complex glomerulonephritis. A dose dependent glomerulonephritis with acute, latent and chronic phases in a long-term study.","authors":"B M Iversen, R Matre, J Ofstad","doi":"","DOIUrl":"","url":null,"abstract":"The pathophysiology, histology and immunohistology of acute and chronic heterologous immune complex glomerulonephritis were investigated in a long-term study in male Wistar rats. The glomerulonephritis showed 3 phases: an initial nephrotic syndrome, a latent phase with stable proteinuria (40 mg/24 h), and a terminal phase with increasing proteinuria and blood pressure, and declining serum protein concentration and creatinine clearance. Antiserum doses of 1.0, 1.5 and 2.0 ml induced maximal proteinuria (112, 257 and 272 mg/24 h respectively) after 14 days whereas normal rabbit serum and 0.5 ml antiserum gave no proteinuria. After 100 days, the rats injected with 1.0 ml of antiserum did not show physiological signs of renal disease; in the rats injected with 1.5 ml of antiserum the disease run a chronic course. Equal amounts of rabbit IgG, rat IgG and rat C3 were found in 10 glomeruli from rats 100 days after injection of 0.5, 1.0 and 2.0 ml (p greater than 0.10). Intramembranous deposits and spike formation were observed in all groups. All changes increased with greater antiserum doses. Chronically diseased animals observed from 500 to 750 days showed deposits of rabbit IgG in the basement membrane, and in most animals small amounts of rat IgG and rat C3 were also observed. This is compatible with a sustained stimulus for antibody formation throughout the course of this type of glomerulonephritis.","PeriodicalId":77653,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","volume":"90 5","pages":"241-50"},"PeriodicalIF":0.0,"publicationDate":"1982-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17281473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Function of polymorphonuclear neutrophilic leucocytes. Comparison of leucocytes from blood and exudate in healthy volunteers. 多形核嗜中性白细胞的功能。健康志愿者血液与渗出液白细胞的比较。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1982-02-01

J H Wandall

{"title":"Function of polymorphonuclear neutrophilic leucocytes. Comparison of leucocytes from blood and exudate in healthy volunteers.","authors":"J H Wandall","doi":"","DOIUrl":"","url":null,"abstract":"The function of polymorphonuclear neutrophils from blood (B-PMN) and from exudates (E-PMN) was studied in healthy volunteers. The E-PMNs were isolated from skin windows with chambers and the chemotactic, phagocytic and Nitro Blue Tetrazolium (NBT) reducing activity measured and compared to that of simultaneously obtained B-PMNs. The chemotactic and random migration of E-PMNs in Boyden chambers measured by the leading front and by the chemotactic index were reduced compared with B-PMNs (p less than 0.01). Serum independent phagocytosis of paraffin oil emulsions was significantly increased (p less than 0.01) by E-PMNs after 12, 24 and 48 hours and not correlated to the function of B-PMNs. Opsonization with autologous serum increased the phagocytosis by both E-PMNs and B-PMNs, but mainly the latter. The NBT reduction by E-PMNs was increased (p less than 0.01) and positively correlated to the NBT reduction by resting B-PMNs. In contrast, there was no difference in NBT reduction between phagocytosing E-PMNs and B-PMNs. Mobilization of PMNs to an inflammatory focus in healthy subjects induces marked changes in the function, and B-PMN function cannot always be assumed to reflect the function of PMNs from inflammatory sites.","PeriodicalId":77653,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","volume":"90 1","pages":"7-13"},"PeriodicalIF":0.0,"publicationDate":"1982-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18091301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0