Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry最新文献

{"title":"[Lectin histochemical study on human dental pulp. Special reference to odontoblasts and pulp cells].","authors":"K Endo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present study investigated some lectin affinities of human dental pulps, especially of odontoblasts and pulp cells. The materials were obtained from clinically intact teeth that were caries-free, attrition and/or abrasion-free. Mucopolysaccharide staining was carried out with applied PAS and alcian blue (AB) (pH 1.0 and 2.5). Lectins used were Con A, WGA, RCA-1, UEA-1, DBA, SBA, MPA, LFA, HPA, PNA, and GS-1, and the avidin-biotin peroxidase complex method was employed. Some specimens were tested for PNA binding after treatment with sialidase. The following results were obtained: 1) On PAS and AB staining, the pulp tissue was very weakly or borderline positive. 2) Lectin binding in odontoblasts was intensely positive with Con A, WGA, RCA-1, MPA, and LFA, but negative or very weakly positive with the other lectins examined. 3) Lectin localization in odontoblasts was localized diffusely throughout the cytoplasm. 4) On PNA staining, odontoblasts were negative, but changed to positive after treatment with sialidase. 5) Odontoblast processes showed negative or borderline staining with all lectins used in this study. 6) The pulp cells were clearly positive with Con A, MPA, LFA, RCA-1, and SBA and especially LFA showed an intense reaction with the pulp cells. 7) WGA affinity for odontoblasts was very strong but that for pulp cells was very weak. 8) Lectin binding in pulp cells was observed mainly in the processes of the cells. From the above results, it is clear that the lectin binding pattern of odontoblasts differs from that of pulp cells. The data suggest that D-mannose, N-acetyl-D-glucosamine, D-galactose, and N-acetyl-D-galactosamine residues are localized in the odontoblasts and sialic acid is localized in the pulp cells.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 3","pages":"360-81"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Study on obturating efficiency of Gutta-percha point as a root canal filler. Difference in results among combinational usages of hand reamers and files].","authors":"M Sato,&nbsp;Y Amano","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The last treatment step followings extirpation of pulp from an infected root canal is root canal filling. Before root canal filling is done, however, the canal must be enlarged enough and the infected dentin and debris removed thoroughly from the canal. Furthermore, re-infection from the apical foramen at the apex of the root canal should be prevented. In this study, we examined the root apex sealing ability of gutta-percha points applied by the lateral condensation method and the vertical condensation method, as well as the difference in root canal sealing ability of gutta-percha points when either a hand reamer or file or their combination was used for root canal enlargement. Extracted human upper incisors were used for root canal enlargement. The condition of the root canal wall after enlargement was observed by scanning electron microscope. The fitness between the wall and gutta-percha was determined by observation of replicas filled with gutta-percha. Furthermore, to assess the degree of apex sealing after root canal filling, we immersed the extracted teeth in Indian ink for 1 day, 3 days, 1 week, 2 weeks, or 4 weeks (Indian ink permeation test). The results obtained were as follows: 1. Reamer and K-file could smooth the root canal wall more than H-file by the scratch-up method. And also the instruments used by the rotation method formed a sure apical seat, which resulted in film apex filling. 2. By the observation of gutta-percha point-filled replicas we noted a border line between the apical seat and apical foramen when the lateral condensation method was used, whereas the line was not observed with the vertical condensation method. 3. Between the 2 filling methods there was no difference in the ink permeation by Indian ink permeation test, but high sealing ability was observed in the root canal in which a sure apical seat had been formed.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 3","pages":"390-410"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Biological characterization of Bacteroides intermedius specific monoclonal antibody].","authors":"T Satoh,&nbsp;S Hanazawa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>One monoclonal antibody (BIF6) directed against Bacteroides intermedius Group I was developed by hybridoma technology. In the present study, we used culture medium from BIF6 antibody-producing cells to characterize the antibody. Its reactivity against B. intermedius (Group I, II), B. gingivalis, B. corporis, B. melaninogenicus, B. loescheii, and B. asaccharolyticus was detected by the enzyme-linked immunosorbent assay. BIF6 monoclonal antibody specifically reacted with B. intermedius Group I, but not with B. intermedius Group II. Also, the BIF6 did not react with other black-pigmented Bacteroides. Since BIF6 antibody reacted with some clinical isolates from subgingival plaques of adult periodontal patients, we suggest that this antibody may be useful as a tool in the clinic to identify B. intermedius Group I. Specific antigen of B. intermedius Group I recognized by BIF6 antibody was located on the outer membrane of the organism. And the specific antigen was inactivated by treatment for 20 min at 80 degrees C and at pH2 and 12. Also the antigen was sensitive to trypsin treatment.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 2","pages":"187-96"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Some properties of hydrophilic addition-type silicone impression materials].","authors":"T Tokushima,&nbsp;K Nagayama,&nbsp;T Tawaragi,&nbsp;H Hashimoto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Addition-type silicone impression materials have the characteristic of low wetting by water; therefore, they are sometimes inconvenient in clinical use. However, hydrophilic addition-type silicone impression materials are said to be improved in this characteristic. In this study, the physical properties of two kinds of hydrophilic addition-type silicone impression materials, namely, Hydrophilic EXAFLEX and EXPRESS, were investigated and compared with the traditional addition-type silicone impression materials. Compression set, strain in compression, consistency, and contact angles of the salivary droplet on the these impression materials were measured in accordance with ADAS no. 19. The following results were obtained: 1. Each compression set of HEM, HEL, EPM, EPL, EPE (hydrophilic addition-type silicone impression materials), having superior property in compression recovering, was less than 0.1%, they were equivalent to one-seventh of that of the traditional addition-type silicone impression materials. 2. Strain in compression of HEM, HEL, EPM, EPF was bigger than that of the traditional addition-type silicone impression materials. 3. Consistency of HEM, HEL, EPM, EPL, EPF was smaller in fluidity than that of the traditional addition-type silicone impression materials. Among all impression materials in this study, only with HEM and HEL could a difference in consistency between wet films and dry films be recognized. 4. Contact angles of all hydrophilic addition-type silicone impression materials were smaller than those of the traditional addition-type silicone impression materials. They were equivalent to two-thirds of that of the traditional addition-type silicone impression materials, and improvement in wetting property of these hydrophilic addition-type silicone impression materials was observed.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 2","pages":"205-12"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Long-term post-treatment observation of a case with orthodontic treatment using occipito-mental anchorage only].","authors":"R Nakamura,&nbsp;M Nakamura,&nbsp;O Yoshimura,&nbsp;J Abe","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Occipito-mental anchorage (OMA) was used in a skeletal class III case with maxillary retrognathism. Long term observation of the patient post treatment revealed the following: 1. The facial profile was improved gradually until the end of treatment: but by 5 years after treatment, it had become slightly worse. 2. Improvement was seen in the maxillomandible relationship owing to the continuous slight advancement of the maxilla and the inhibition of the advancement of the mandible. And this relationship also showed long-term stability after treatment. Hardly any rotation of the mandible was found during the treatment. But clockwise rotation of the palatal plane during treatment and counterclockwise rotation after treatment were observed. 3. Regarding dental changes, labial inclination of upper incisors and lingual inclination of lower incisors owing to the improvement of overjet were seen during treatment. But they reverted to almost the same inclinations seen at the first examination by 5 years after treatment.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 2","pages":"276-86"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on vibrations of the mandible.","authors":"Y Okumura,&nbsp;H Yamada,&nbsp;N Takahashi,&nbsp;Y Kanai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In order to elucidate the phenomenon of vibrations of the mandible, a simplified plaster model was prepared and the fundamental vibrations were investigated. Studies using either dried human skulls or in situ mandible yielded similar results. The results obtained were as follows: 1. The vibrations generated by tapping a simplified mandible model were similar to those of the transverse type of a bar and tuning fork. 2. Both vibration models belonged to the transverse type bar vibrations. 3. Similar results were obtained by tapping the teeth of the mandible in situ. 4. The presence of fractures and cysts in the mandible might induce changes in vibrations.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 1","pages":"74-81"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13744934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Gingival response by the effect of brushing method and hardness of the toothbrush bristle].","authors":"K Iwakami,&nbsp;Y Watanabe","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of toothbrushing is plaque removal and gingival massage. However, intensive oral hygiene frequently results in gingival damage. The object of this study was to determine the damage to oral soft tissues resulting from three methods of brushing (roll, scrub, and bass method) and the use of toothbrushing with two different hardness (Hard: 10.2 kg/head, Soft: 3.2 kg/head). Six monkeys were used for this study. The mouth of each was divided into four sections: upper right for a control, upper left for the scrub method, lower right for the roll method, and lower left for the bass method. The roll method was run at 10 strokes per 10 seconds with a load of 800 grams, and the scrub and bass methods were run at 30 back and forth strokes per 10 seconds, with a load of 400 grams for each method. During the experimental period of five months, brushing by each of the three methods were done once a day. The gingival index, the gingival bleeding index, pocket depth, width of attached gingiva, and gingival recession were measured regularly. Analysis was performed by analysis of variance (ANOVA). The scrub method resulted in greater gingival recession and, at the same time, loss of attached gingiva than the roll and bass methods. The use of the hard brush resulted in greater gingival recession and loss of attached gingiva than the use of the soft brush. With the three methods of brushing, a significantly greater distance of gingival recession and loss of attached gingiva were recorded after brushing by the scrub method than by the roll and bass methods. The study also showed that the modest gingival recession and reduction width of attached gingiva seen with the scrub method and increased stiffness of toothbrush bristles were accompanied by increased damage to the soft gingival tissues.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 2","pages":"244-66"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Inducing effect of periodontopathic bacteria on activation of macrophage functions and production of interleukin-1 by mouse peritoneal macrophages].","authors":"K Honda,&nbsp;Y Ohmori","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Periodontal disease is thought to be initiated by a bacterial infection and subsequently developed by immunopathological mechanisms thorough host-parasite interactions. The macrophage and lymphocyte are the major functional cell types in the lesion of the disease and participate in tissue destruction and alteration of the periodontal connective tissue as well as in host defense mechanisms. However, the detailed implications of macrophages in development of the disease is still unclear. The aim of this study was to gain more understanding of the functional role of macrophages in periodontal disease. In this study, we examined the inducing effects of sonicated extracts from some gram-negative and gram-positive bacteria associated with the pathogenesis of periodontal disease, including Bacteroides gingivalis, Fusobacterium nucleatum, Haemophilus actinomycetemcomitans, and Actinomyces viscosus, on activation of macrophage functions and IL-1 production by the macrophages from the mouse peritoneum. At a dose as low as 1 microgram/ml (dry weight) sonicated extracts from B. gingivalis induced an increase in acid phosphatase activity and in glucose consumption of mouse peritoneal macrophages in vitro. A significant increase in the acid phosphatase and in glucose consumption was observed in the cultures at 24 h and 48 h, respectively, after the addition of the sonicate. Sonicated extracts from A. viscosus, a gram-positive bacterium, as well as B. gingivalis, F. nucleatum, and H. actinomycetemcomitans, gram-negative ones, were able to induce the increase in acid phosphatase activity and in glucose consumption of the macrophages. These periodontopathic bacteria were found to strongly induce IL-1 production by the macrophages as early as 24 h after addition of the sonicates. A significant increase in the IL-1 production was observed at a dose of 1 microgram/ml of the sonicates. The inducing ability was equivalent to 1 microgram/ml Escherichia coli lipopolysaccharide. The highest production of IL-1 was observed in the macrophages treated with H. actinomycetemcomitans among these sonicates. Sonicated extracts from both gram-negative and gram-positive bacteria were able to induce the IL-1 production by macrophages from C3H/HeJ mice, which are LPS low-responders. These results suggest that periodontopathic bacteria have potent ability to induce macrophage activation and IL-1 production and that the activated macrophages may play an important role in development of periodontal disease.(ABSTRACT TRUNCATED AT 400 WORDS)</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 1","pages":"1-20"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13744195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Genetic study of ovary development in Drosophila lutescens].","authors":"A Fukatami","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Experiments were carried out in order to clarify the genetic mechanism governing the development of ovaries of Drosophila lutescens female flies. Female flies of two strains obtained from northern (Niigata) and southern (Tokunoshima) parts of Japan, F1, F2, and backcrossed hybrids were examined. Female flies, one week after emergence at 14 degrees C, during which time they were kept under a 10 h 30 min light/13 h 30 min dark regimen, were dissected, and they were divided into three classes: those with mature, retarded, and undeveloped ovaries. The ratio of undeveloped ovary in the northern strain was much higher than that of the southern. From the results of all dissections, it is assumed that genes on chromosomes of the northern strain operate to stop ovarian development and that cytoplasmic factors of the southern strain act to suppress those genes. It may be, therefore, suggested that the development of the ovary of this species before hibernation is controlled genetically.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 1","pages":"131-6"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13744196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The effect of fluoride-containing tooth paste on dental plaque and on fluoride level in the mouth].","authors":"H Oomori","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Various kinds of fluoride have been used for a long time and there are many reports concerning fluorides and their effects. Recently, the caries-inhibiting action of fluoride-containing tooth paste has been given much attention. In this study, I tried to clarify the residual time and amount of fluoride derived from the fluoride-containing tooth paste in the mouth, as well as to assess possible variation in bacterial composition in the dental plaque bacteriologically and biochemically. In the study on the fluoride clearance from the mouth, both 1.0 g and 0.5 g of paste showed the same reduction rates; and about an 80% reduction was recognized between the value at 3 minutes and that at 30 minutes, and about a 40% reduction from the 30-minute to the 60-minute interval. Next, a study on the variation in plaque bacteria was carried out. The total number of the CFU on each plate was not different between samples obtained before and after the use of the tooth paste; moreover, no difference was noted between aerobic and anaerobic culture. However, when plaque before and after brushing with fluoride-containing tooth paste were cultured in 10% sucrose solution, the differences of acid production such as lactic acid, acetic acid, and formic acid were demonstrated. Namely, these acid productions were inhibited after the use of fluoride, especially lactic acid was strongly inhibited. On the other hand, when Str. mutans from the plaque obtained after the use of fluoride-containing tooth paste was cultured in fluoride-free BHI broth, the inhibition of acid from carbohydrates was not shown clearly.(ABSTRACT TRUNCATED AT 250 WORDS)</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 1","pages":"37-46"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13744198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}