{"title":"[中间拟杆菌特异性单克隆抗体的生物学特性研究]。","authors":"T Satoh, S Hanazawa","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>One monoclonal antibody (BIF6) directed against Bacteroides intermedius Group I was developed by hybridoma technology. In the present study, we used culture medium from BIF6 antibody-producing cells to characterize the antibody. Its reactivity against B. intermedius (Group I, II), B. gingivalis, B. corporis, B. melaninogenicus, B. loescheii, and B. asaccharolyticus was detected by the enzyme-linked immunosorbent assay. BIF6 monoclonal antibody specifically reacted with B. intermedius Group I, but not with B. intermedius Group II. Also, the BIF6 did not react with other black-pigmented Bacteroides. Since BIF6 antibody reacted with some clinical isolates from subgingival plaques of adult periodontal patients, we suggest that this antibody may be useful as a tool in the clinic to identify B. intermedius Group I. Specific antigen of B. intermedius Group I recognized by BIF6 antibody was located on the outer membrane of the organism. And the specific antigen was inactivated by treatment for 20 min at 80 degrees C and at pH2 and 12. Also the antigen was sensitive to trypsin treatment.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 2","pages":"187-96"},"PeriodicalIF":0.0000,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Biological characterization of Bacteroides intermedius specific monoclonal antibody].\",\"authors\":\"T Satoh, S Hanazawa\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>One monoclonal antibody (BIF6) directed against Bacteroides intermedius Group I was developed by hybridoma technology. In the present study, we used culture medium from BIF6 antibody-producing cells to characterize the antibody. Its reactivity against B. intermedius (Group I, II), B. gingivalis, B. corporis, B. melaninogenicus, B. loescheii, and B. asaccharolyticus was detected by the enzyme-linked immunosorbent assay. BIF6 monoclonal antibody specifically reacted with B. intermedius Group I, but not with B. intermedius Group II. Also, the BIF6 did not react with other black-pigmented Bacteroides. Since BIF6 antibody reacted with some clinical isolates from subgingival plaques of adult periodontal patients, we suggest that this antibody may be useful as a tool in the clinic to identify B. intermedius Group I. Specific antigen of B. intermedius Group I recognized by BIF6 antibody was located on the outer membrane of the organism. And the specific antigen was inactivated by treatment for 20 min at 80 degrees C and at pH2 and 12. Also the antigen was sensitive to trypsin treatment.</p>\",\"PeriodicalId\":77571,\"journal\":{\"name\":\"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry\",\"volume\":\"18 2\",\"pages\":\"187-96\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1989-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
利用杂交瘤技术制备了一种针对中间拟杆菌(Bacteroides intermedius Group I)的单克隆抗体(BIF6)。在本研究中，我们使用BIF6抗体产生细胞的培养基来鉴定抗体。采用酶联免疫吸附法检测其对中间芽孢杆菌(ⅰ、ⅱ群)、牙龈芽孢杆菌、企业芽孢杆菌、黑素原芽孢杆菌、干酪芽孢杆菌和溶糖芽孢杆菌的反应性。BIF6单克隆抗体对中间芽胞杆菌ⅰ群有特异性反应，对中间芽胞杆菌ⅱ群无特异性反应。此外，BIF6不与其他黑色拟杆菌发生反应。由于BIF6抗体与成人牙周病患者龈下斑块的临床分离物发生反应，我们建议该抗体可作为临床鉴定中间芽胞杆菌I群的工具。BIF6抗体识别的中间芽胞杆菌I群的特异性抗原位于生物体的外膜上。在80℃、pH2和12条件下灭活特异性抗原20 min。该抗原对胰蛋白酶处理敏感。
[Biological characterization of Bacteroides intermedius specific monoclonal antibody].
One monoclonal antibody (BIF6) directed against Bacteroides intermedius Group I was developed by hybridoma technology. In the present study, we used culture medium from BIF6 antibody-producing cells to characterize the antibody. Its reactivity against B. intermedius (Group I, II), B. gingivalis, B. corporis, B. melaninogenicus, B. loescheii, and B. asaccharolyticus was detected by the enzyme-linked immunosorbent assay. BIF6 monoclonal antibody specifically reacted with B. intermedius Group I, but not with B. intermedius Group II. Also, the BIF6 did not react with other black-pigmented Bacteroides. Since BIF6 antibody reacted with some clinical isolates from subgingival plaques of adult periodontal patients, we suggest that this antibody may be useful as a tool in the clinic to identify B. intermedius Group I. Specific antigen of B. intermedius Group I recognized by BIF6 antibody was located on the outer membrane of the organism. And the specific antigen was inactivated by treatment for 20 min at 80 degrees C and at pH2 and 12. Also the antigen was sensitive to trypsin treatment.
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