Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry最新文献

{"title":"[Measurement of oral and facial blood flow with a laser Doppler flowmeter. 4. Alteration of oral blood flow by position change].","authors":"T Shimazaki,&nbsp;J Shimada,&nbsp;Y Yamazaki,&nbsp;M Okitsu,&nbsp;Y Hiranuma,&nbsp;M Eba,&nbsp;E Sakamoto,&nbsp;Y Yamamoto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The laser doppler flowmeter was developed by Stern in 1975 and applied for practical use by Nilsson et al. in 1980. Since then, it has been used in various fields of medicine. The primary advantages of this device an that it is noninvasive and can monitor the blood flow continuously and rapidly. We used a Laser doppler flowmeter to continuously monitor the change in the oral peripheral blood flow due to changes in the body position of patients in a dental chair, since such changes are unavoidable during the course of dental treatment. A change from the sitting position to the supine position caused an increase in oral blood flow in 19 of 20 patients. In terms of the mean value per minute, oral blood flow was 23% greater in the supine position.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 3","pages":"302-6"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Production of IL-1 like cytokine by cultured bone cells: inducing effect of Haemophilus actinomycetemcomitans lipopolysaccharide on the cytokine production].","authors":"K Yoshimura,&nbsp;S Hanazawa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have studies the role that interleukin-1 (IL-1) and IL-1-like cytokine play in the mechanism of bone resorption that occurs in periodontal disease. To determine whether the bone cell itself produces IL-1-like cytokine, we examined bone cells migrating from fragments of newborn mouse calvaria. These bone cells were cultured in alpha-MEM with of without fetal calf serum. IL-1-like cytokine activity was measured by incorporation of [3H] thymidine into C3H/HeJ thymocytes treated with lipopolysaccharide (LPS) from Haemophilus actinomycetemcomitans, a bacterium found in juvenile periodontopathy. The bone cells produced a significant amount of IL-1-like cytokine. The maximum production of IL-1-like cytokine was observed at 24 hours with the LPS in serum-free alpha-MEM. IL-1-like cytokine production stimulated by LPS was marked in the bone cells from LPS high-responder C3H/HeN mice, but not in those from low-responder C3H/HeJ mice. Peak of IL-1-like cytokine activity in culture supernatants of the bone cells was detected in fractions with a molecular weight corresponding to 15,000 daltons.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 3","pages":"420-9"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanical study on the stability of multiform mini-plates. I. Double-T-type plate (90, regular, 6-holes) in mandibular body fracture].","authors":"T Kameyama,&nbsp;E Sakamoto,&nbsp;H Takeshima,&nbsp;J Shimada,&nbsp;Y Yamamoto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this present paper, we reported the stability of osteosynthesis using a special type of mini-plate for mandibular fractures as evaluated from a mechanical study with static loading. The materials used in this study were fifteen bars made of acrylic resin, each divided at the center of its length into two parts to simulate a condition of mandibular body fracture. Following the fixation of these materials with mini-plates firmly attached between their cut ends, the bars were secured in a vice at one end and a static load was applied from the vertical direction at the other end. The amount of displacement of the loaded end was investigated with a displacement-measuring device, and the strains on mini-plates and test bars were measured with strain gauges. A comparative study was conducted on three kinds of mini-plates: 1) Group S, fixed with one Champy mini-plate, 2) Group W, fixed with two Champy mini plates, 3) Group T, fixed with one double-T-type mini-plate. The following results were obtained: 1) The amount of displacement was largest in the vertical direction and smallest in the horizontal direction in the three groups. Especially in group T, it was smaller the longitudinal and horizontal axes than in the other two groups. 2) Longitudinal strain of the plate was smallest in group W. However, the strain on the test bar was smallest group S. Especially , in group T, the largest compressive strain was observed at the lower portion of the synthesized ends.(ABSTRACT TRUNCATED AT 250 WORDS)</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 1","pages":"88-96"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13744936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant interferon-gamma is a potent inhibitor of osteoblastic cell functions.","authors":"K Hirose,&nbsp;S Hanazawa,&nbsp;S Amano,&nbsp;H Kikuchi,&nbsp;A Takeshita,&nbsp;Y Murakami,&nbsp;H Hashimoto,&nbsp;T Yokoyama,&nbsp;R Suyama,&nbsp;S Kitano","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Interleukin-1 and tumor necrosis factor-stimulated bone resorption is mediated by osteoclast-activating factor elaborated by osteoblasts. Recombinant interferon-gamma inhibits stimulation of bone resorption by these cytokines. We examined here the effects of recombinant mouse interferon-gamma (rmIFN-G) on DNA and collagen synthesis, and on alkaline phosphatase (ALP) activity, in the osteoblastic cell line (MC3T3-E1) under confluent culture conditions. Addition of rmIFN-G to the cells markedly inhibited their DNA synthesis and ALP activity in dose- and culture time-related manner. Also we found that rmIFN-G decreased markedly collagen synthesis at day 3 after addition of the agent. These data indicate that rmIFN-G is a potent inhibitor of osteoblastic cell functions.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 3","pages":"296-301"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13663145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Histopathological and immunohistochemical studies on mucous cysts].","authors":"N Kuroda","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present study investigated the histopathology, histochemistry of mucopolysaccharides, and immunohistochemistry of oral mucous cysts. The materials were obtained from ninety cases that were histopathologically diagnosed as oral mucous cysts at the Department of Oral Pathology, Meikai University School of Dentistry. Mucopolysaccharide staining was done with PAS, alcian blue (AB, pH 2.5) and high iron diamine (HID). Immunohistochemical studies were focused on secretory component (SC), lactoferrin (Lf), alpha-amylase (Am), IgA, lysozyme (Ly), and keratin (Kr). The following results were obtained: 1. Histopathological findings. (1) Retention and/or retention-like type cysts occurred in was twenty-six cases and the extravasation type in sixty-four cases. (2) Cases showing epithelial lining of the cystic wall were only eight in number, and many cystic walls were contained granulation tissue (fifty cases). (3) As for inflammation of the cystic wall, the degree was slight, and infiltrated cells were mainly macrophages (so-called mucinophages) and lymphocytes. (4) Regarding adjoining salivary glands, acinar cells showed atrophic changes, and hypertrophy of mucous acinar cells was evident. Many ducts showed dilatation, and stromal connective tissue showed fibrosis and hyalinization. 2. Histochemical findings on mucopolysaccharides. (1) Mucous materials in cystic cavity, mucous acinar cells, and secretory materials in ductal lumens were intensely stained by PAS and AB. But stainability with AB was less than that with PAS staining. Serous acinar cells and ductal epithelium were negative to PAS and AB staining. (2) Stainability of the above with HID was less than at with PAS or AB. Cystic walls were not stained by HID. Mucous acinar cells reactive with HID were intensely stained, but the number of the positive cells was limited when compared with the numbers of PAS-and AB-positive cells. 3. Immunohistochemical findings. (1) As for mucous materials in the cystic cavity, reactions for Sc, Am, IgA, and Ly were all positive, whereas those for Lf and Kr were negative. (2) Staining of cystic walls was generally weak: the walls were negative for IgA, Ly and Kr showed and borderline or slightly positive for Sc, Lf, and Am. (3) Mucous acinar cells were negative for all markers examined in this study, but serous acinar cells and/or demilunes were markedly positive for Sc, Ly, and Am. (4) In ductal epithelial cells, Ly and Kr were negative, but IgA was borderline or slightly positive. Sc and Am gave intensely positive staining. (5) Secretory materials in ductal lumens, Sc were intensely positive for Lf, and Ly; slightly or moderately positive for IgA; and slightly positive or negative for Am.(ABSTRACT TRUNCATED AT 400 WORDS)</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 3","pages":"337-59"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13632397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Five cases referred to the First Department of Oral Surgery due to failure of impacted mandibular third molar extraction].","authors":"Y Hiranuma,&nbsp;M Okitsu,&nbsp;H Harada,&nbsp;Y Katoh,&nbsp;T Shimazaki,&nbsp;M Eba,&nbsp;Y Yamamoto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Of patients referred to our department over the post two years due to failure of tooth extraction 5 with impacated mandibular third molars were treated. We found that extraction had been hindered by the narrow operative field due to inadequate incision of the overlying mucosal tissue, insufficient bone resection and inadequate separation of the crown and root.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 3","pages":"430-7"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Study on retention of veneering materials to the facing crown made of hard-type resin. Application etching to Ni-Cr alloys].","authors":"M Ichikawa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In order to enhance adhesiveness between the resin and the metal in the facing crown made of hard-type resin, the bead application method has been widely utilized. A short-coming of this method is that the metal part of the facing must be made thicker for bead application. If, however, a retention is made beneath the surface of the metal part, the facing metal can be made thinner. The author studied the adhesiveness of metal to resin by producing pitting corrosion on the surface of four different kinds of Ni-Cr alloys, etching the surface with strong acid under various conditions, and compared the adhesiveness and failure conditions with those of the beaded specimens. The results were as follows: 1) The degree of corrosion of the surface of specimens was positively correlated with the duration of etching. However, the degree of corrosion differed depending on the kinds of etching solutions and specimen alloys. 2) In terms of adhesiveness, some of the etching groups were stronger than the beaded groups and some were weaker, depending on the kinds of alloys and etching conditions. 3) Condition of failure at the joint surface: in the beaded groups cohesive failure was the most prevalent type. In the etching groups, compound failure was the most prevalent. There was a positive correlation between adhesive strength and the amount of residual resin: the more residual resin, the greater the adhesive strength. 1) Electron microscopic observations: on the surface of specimens of the etching groups pitted roughness was observed, and the resin material was tightly attached to the metal surface. However, reciprocal diffusion of the elements from both materials was not indicated, nor was there any evidence of chemical bonding.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 2","pages":"213-33"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Studies on the relation between glucose metabolism and c-AMP formation in dental pulps in the presence of inflammatory chemical mediators in vitro].","authors":"H Kiyohara","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The relationship between glucose metabolism and cyclic-AMP production in dental pulp in the presence of chemical mediators was investigated in vitro. It is generally accepted that oxidation of glucose-6-14C is indicative of metabolism by the glycolytic pathway whereas that of glucose-1-14C occurs by the hexose monophosphate shunt. The 14CO2 productions from both routes were compared in dental pulp from cattle and rats in the presence of each of several chemical mediators: bradykinin (1.7-3.3 micrograms/ml), prostaglandin E1 (0.3 micrograms/ml), prostaglandin E2 (0.3 micrograms/ml), histamine (33 micrograms/ml), and 5-hydroxytryptamine (33 micrograms/ml). The effects of dental filling materials on glucose oxidation, and cyclic-AMP production by chemical mediators in pulp tissues were also investigated. The results obtained were as follows: 1) Glucose oxidation in dental pulp was stimulated by chemical mediators generally by way of the Embden-Meyerhof Parnas pathway, and was further stimulated by the medium containing bradykinin. However, it was depressed in the presence of higher concentrations of chemical mediators, especially depressed in the HMS pathway. 2) The oxidation ratio of glucose-1-14C to glucose-6-14C (G1/G6) in dental pulp was 4 to 8 in the cattle and 0.6 in the rat, showing clear differences in glucose oxidation between the two animals. 3) Moreover, glucose oxidation in rat dental pulp was 60 to 80 times higher in the EMP pathway and 5 to 10 times higher in the HMS pathway than those in the cattle. 4) Dental filling materials such as silicate cement, zinc phosphate cement, calcium hydroxide, and eugenol cement severely depressed glucose-6-14C oxidation in bovine dental pulp when used at high concentrations, but not at low concentrations. 5) The chemical mediators tested in this experiment (PGE1, PGE2, histamine, 5-HT, bradykinin, and substance P) stimulated cyclic AMP production in rat dental pulp. The production was highest with PGE1 and PGE2. The cyclic AMP production was further stimulated by addition of histamine or 5-HT to the medium containing PGE1 or PGE2.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 2","pages":"171-86"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13705919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical prospective study of surgical treatment of pterygium].","authors":"R Chujo,&nbsp;T Tashiro,&nbsp;H Masuda,&nbsp;T Ichioka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pterygium is a kind of degenerative lesion of the ocular conjunctiva and is thought to be hyperaemia of fibrovascular tissue onto the cornea caused by various factors. The most frequently used treatment for pterygium at present is surgery. Various surgical procedures and postoperative treatments for pterygium have been reported, however, the postoperative recurrence rates are somewhat high. Therefore, until now we have had no satisfactory clinical management of pterygium. The results of our method for surgical treatment of pterygium are given and compared with those described in many other reports. From this comparison, our clinical surgical treatment of pterygium, which is the so-called \"bare sclera\" technique, is evaluated to give the best management without serious complications and recurrences.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 1","pages":"47-52"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13744199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of experimental diabetes on epidermal growth factor (EGF) receptors in the rat liver].","authors":"Y Natsumi,&nbsp;M Kashimata,&nbsp;M Hiramatsu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Epidermal growth factor (EGF) is now well known as a potent mitogen and differentiation factor for a variety of cells both in vivo and in vitro. Like other polypeptide hormones, EGF initially binds to a specific plasma membrane receptor on the target cells. In this study, we investigated the effect of streptozotocin-induced diabetes on EGF receptors on rat liver plasma membranes. An apparent increase in serum glucose concentration was observed in diabetic rats, and treatment of diabetic animals with insulin normalized the glucose concentration to the control level. There was no marked difference in hepatic membrane markers among the control, diabetic and insulin-treated diabetic animals, as judged by protein, sialic acid contents, and phosphodiesterase I and 5'-nucleotidase activities. The binding of 125I-EGF to membranes was found to be significantly lower in diabetic than in control animals. The value in diabetic animals was about 55% of the control level. Insulin treatment of diabetic animals restored the binding of 125I-EGF to the control level, whereas triiodothyronine (T3) treatment had no effect. Scatchard analysis of the binding data clearly showed that the decrease in EGF binding was due to a decrease in the number of receptors rather than to a change in receptor affinity. The decrease in EGF receptor number in diabetic animals was also confirmed by an experiment on affinity labeling of EGF receptors. EGF stimulated the phosphorylation of hepatic EGF receptors (molecular weight = 170,000). The rates of basal and EGF-stimulated phosphorylation of the receptors were lower in diabetic than in control animals. Insulin treatment of diabetic animals restored the phosphorylation activity to control level, whereas T3 treatment had no apparent effect. There was no significant difference in serum EGF concentration among the control, diabetic and insulin-treated diabetic animals. These results indicate that insulin deficiency in vivo causes a decrease in hepatic EGF receptor number, and suggest that the actions of EGF on hepatocytes may also be affected by diabetes mellitus since the effects of EGF are receptor-mediated.</p>","PeriodicalId":77571,"journal":{"name":"Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry","volume":"18 1","pages":"21-36"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13675936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}