Eksperimental'naia onkologiia最新文献

Downregulation of estrogen receptor alpha and beta expression in carcinogen-induced mammary gland tumors of rats. 雌激素受体α和β在癌变大鼠乳腺肿瘤中的表达下调。

Eksperimental'naia onkologiia Pub Date : 2004-03-01

Jin Seok Kang, Na Jin Jung, Seyl Kim, Dae Joong Kim, Dong Deuk Jang, Ki-Hwa Yang

{"title":"Downregulation of estrogen receptor alpha and beta expression in carcinogen-induced mammary gland tumors of rats.","authors":"Jin Seok Kang, Na Jin Jung, Seyl Kim, Dae Joong Kim, Dong Deuk Jang, Ki-Hwa Yang","doi":"","DOIUrl":"","url":null,"abstract":"Aim and methods: The recent discovery of a new isoform of estrogen receptor (ER), ER beta, has promoted the investigation of its expression on mammary gland. This study was carried out to examine the expression of ER alpha, ER beta and proliferating cell nuclear antigen (PCNA) in the carcinogen-induced mammary tumors induced by N-methyl-N-nitrosourea (MNU) or 7,12-dimethylbenz[a]anthracene (DMBA), and to compare these expression with those of age-matched normal mammary glands.Results: There was significant decrease of expression of ER alpha and ER beta in the mammary gland tumors compared with age-matched normal mammary glands (p < 0.05). In mammary gland tumors, ER alpha expression was mainly located in epithelial cells, showing intranuclear staining pattern. The decrease of ER beta expression was so distant that some tumor cells did not show any expression. There was a complete loss of ER beta expression in 50% (7/14) of MNU-induced mammary gland tumors, and 68.2% (15/22) of DMBA-induced mammary gland tumors. However, there was no difference in PCNA expression between mammary gland tumors and normal mammary glands.Conclusion: This study represents that the decrease of expression of ER alpha and ER beta is associated with mammary carcinogenesis, and suggests that modulation of ER alpha and ER beta may be the target for the treatment of mammary gland tumors.","PeriodicalId":77530,"journal":{"name":"Eksperimental'naia onkologiia","volume":"26 1","pages":"31-5"},"PeriodicalIF":0.0,"publicationDate":"2004-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24492071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of antitumor response to sarcoma 45 in rats by combination of whole-body hyperthermia and interleukin-2. 全身热疗联合白介素-2增强大鼠对45型肉瘤的抗肿瘤反应。

Eksperimental'naia onkologiia Pub Date : 2004-03-01

Michael P Potapnev, Yuri P Istomin, Reiman S Ismail-zade, Edvard A Zhavrid

引用次数: 0

Detection of polyomavirus DNA in human brain tumors. 人脑肿瘤中多瘤病毒DNA的检测。

Eksperimental'naia onkologiia Pub Date : 2004-03-01

Iryna G Vasilyeva, Mikhail I Shamaev, Alexandr Ya Glavatskiy, Natalya G Chopick, Natalya P Olexenko, Olga I Tsyubko, Elena S Galanta, Tatyana A Malisheva

引用次数: 0

Characterization of a new type HPV16 E7 variant isolated from cervical cancer highest incidence area in Hubei Province of China. 湖北省宫颈癌高发区hpv16e7新型变异株的鉴定

Eksperimental'naia onkologiia Pub Date : 2004-03-01

Hui Li, Min Zhao, Xiaoping Qiu, Xiaohua Ding, Yun Tan, Xinxing Wu

{"title":"Characterization of a new type HPV16 E7 variant isolated from cervical cancer highest incidence area in Hubei Province of China.","authors":"Hui Li, Min Zhao, Xiaoping Qiu, Xiaohua Ding, Yun Tan, Xinxing Wu","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To investigate the variation and biological properties of HPV16 E7 isolated from cervical cancer biopsy samples from highest incidence area in HuBei province of China.Methods: HVP16 E7 sequences isolated from the cervical cancer biopsies of 10 local patients were amplified, sequenced and compared with prototype E7 gene. Then the variant gene was cloned into different vectors to study the antigenicity, expression and immunogenicity of its protein by Western blot, immunofluorescence and genetic immunization in vitro or in vivo.Results: The results showed that 7 of 10 samples had the same mutations which led to a nonsense mutation at codon 43 of E7 sequence. The truncated E7 protein could be recognized by standard E7 monoclonal antibody in Western blot and expressed in NIH3T3 cells. In the blood sera of mice immunized intramuscularly by the plasmid DNA expressing the variant E7 gene specific E7 antibodies could be detected at week 2, 3, 5 and 6 after inoculation. However, no specific lymphoproliferation after E7 protein stimulation in vitro was detected by MTT colorimetric assay in comparison to the prototype E7 protein.Conclusion: HPV16 E7 gene may show variation in China and the variant protein could be expressed and induce host humoral immune response, but could not elicit special cellular-immune response against it. These data might hold the key for future development of HPV16 vaccine in HuBei province of China.","PeriodicalId":77530,"journal":{"name":"Eksperimental'naia onkologiia","volume":"26 1","pages":"48-54"},"PeriodicalIF":0.0,"publicationDate":"2004-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24492566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of two different high doses of irradiation on antioxidant system in the liver of guinea pigs. 两种不同高剂量辐照对豚鼠肝脏抗氧化系统的影响。

Eksperimental'naia onkologiia Pub Date : 2004-03-01

Yildiz Guney, Neslihan Bukan, Aysen Dizman, Ayse Hicsonmez, Ayse Bilgihan

{"title":"Effects of two different high doses of irradiation on antioxidant system in the liver of guinea pigs.","authors":"Yildiz Guney, Neslihan Bukan, Aysen Dizman, Ayse Hicsonmez, Ayse Bilgihan","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To examine the state of the oxidant-antioxidant system in the liver of guinea pig caused by high doses of ionizing radiation in the early period.Methods: The research was carried out on guinea pigs irradiated with the doses of 8 Gy (group 2) or 15 Gy (group 3) (single dose/whole body) in comparison with control group (group 1). The levels of thiobarbituric acid reactive substances (TBARS) and glutathione (GSH), the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and the levels of selenium in the liver were measured.Results: TBARS levels in the irradiated animals were markedly higher than those in controls. In group 3, GSH levels and GSH-Px activity were significantly increased while activity of SOD and CAT were significantly decreased compared to groups 1 and 2. Liver selenium levels were not influenced by irradiation.Conclusion: The data have shown that gamma-irradiation at the doses of 8 Gy or 15 Gy results in significant increase in free radical formation while antioxidant enzymes were affected only at a dose of 15 Gy.","PeriodicalId":77530,"journal":{"name":"Eksperimental'naia onkologiia","volume":"26 1","pages":"71-4"},"PeriodicalIF":0.0,"publicationDate":"2004-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24492570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Poly-drug cancer therapy based on ceramide. 基于神经酰胺的多药癌症治疗。

Eksperimental'naia onkologiia Pub Date : 2004-03-01

Norman S Radin

{"title":"Poly-drug cancer therapy based on ceramide.","authors":"Norman S Radin","doi":"","DOIUrl":"","url":null,"abstract":"Thousands of research studies have reported that many kinds of cancer cells and tumors can be killed by treatments that increase the concentration of a simple cellular sphingolipid, ceramide (Cer). While there are many ways to elevate tumor Cer levels, this approach is complicated by the central, complex role of Cer in cell homeostasis: Cer is readily metabolized to form other sphingolipids that increase the tumor's growth rate, metastasis, and resistance to the patient's immune system. This review points out the need to prevent this metabolic conversion while simultaneously stimulating the enzymes that increase the formation of Cer. I describe here many of the enzymes that need stimulation or inhibition, and drugs or metabolites or dietary components that modify each of the enzymes. The review also points to the importance of the allylic alcohol group in Cer and in many cancer drugs, suggesting that the hydroxyl group participates in phosphate transfer to and from proteins by forming a temporary phosphate ester. The allylic hydroxyl may also reduce the ketone moieties in mitochondrial ubiquinone, with formation of reactive oxygen species and apoptogenic breakdown. The level of Cer in tumors can be increased by: (1) direct administration of Cer or a Cer analogue, and (2) stimulation of Cer synthesis from its elementary precursors, or from (3) sphingomyelin by hydrolysis, or from (4) the glucosphingolipids by hydrolysis, or (5) by acylation of sphingosine. In addition, Cer concentration can be raised by slowing its conversion to (6) sphingomyelin, (7) glucosylCer, (8) Cer phosphate, and (9) sphingosine + fatty acid by hydrolysis. Therapeutic radiation stimulates the de novo synthesis of Cer in tumors. Conversion of sphingosine (from Cer) to sphingosine phosphate probably also ought to be blocked.","PeriodicalId":77530,"journal":{"name":"Eksperimental'naia onkologiia","volume":"26 1","pages":"3-10"},"PeriodicalIF":0.0,"publicationDate":"2004-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24491583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental immunotherapy of mice with transplanted MC-rhabdomyosarcoma resistant to doxorubicin. 多柔比星耐药小鼠移植mc -横纹肌肉瘤的实验性免疫治疗。

Eksperimental'naia onkologiia Pub Date : 2004-03-01

Ninel M Berezhnaya, Elena V Kovalchuk, Yulya D Vinnichuk, Svetlana I Spivak, Oxana B Belova

引用次数: 0

Oxidative metabolism of rat blood in the course of alveolar hepatic carcinoma PC-1 growth. 肺泡型肝癌PC-1生长过程中大鼠血液氧化代谢的变化。

Eksperimental'naia onkologiia Pub Date : 2004-03-01

R M Smolyakova, V I Prokhorova, E A Zhavrid, A A Mashevsky, Yu P Istomin

{"title":"Oxidative metabolism of rat blood in the course of alveolar hepatic carcinoma PC-1 growth.","authors":"R M Smolyakova, V I Prokhorova, E A Zhavrid, A A Mashevsky, Yu P Istomin","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To evaluate oxidative metabolism of rat blood in the course of alveolar hepatic cancer growth in vivo.Methods: The oxidation imbalance was assessed by the rise in the values of the integral index of oxidation stress. The structural and functional state of erythrocyte membranes was investigated by spin electron paramagnetic resonance spectroscopy.Results: The growth of alveolar carcinoma was found to be associated with intensification of lipid peroxidation processes with increased blood content of conjugated dienes, malonic dialdehyde against the background of decreased concentration of endogenous antioxidants tocopherol and retinol. Destabilization of the structural state of erythrocyte membranes of rat tumor hosts at the development of oxidation stress was studied, which was characterized by nonspecific structural changes of membrane sorption centres, reduction in specific capacity in the protein-lipid contact area and its increase in the phospholipid bilayer, rise in the degree of order and polarity.Conclusion: Alveolar carcinoma growth in rats resulted in intensification of free radical lipid peroxidation processes with a shift of the prooxidant-antioxidant balance to the left and development of oxidation stress.","PeriodicalId":77530,"journal":{"name":"Eksperimental'naia onkologiia","volume":"26 1","pages":"36-9"},"PeriodicalIF":0.0,"publicationDate":"2004-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24492072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear factor-kappa B regulates cyclooxygenase-2 expression and cell proliferation in human colorectal carcinoma tissue. 核因子κ B调节人结直肠癌组织中环氧合酶-2的表达和细胞增殖。

Eksperimental'naia onkologiia Pub Date : 2004-03-01

Liang-Liang Yu, Hong-Gang Yu, Jie-Ping Yu, He-Sheng Luo

{"title":"Nuclear factor-kappa B regulates cyclooxygenase-2 expression and cell proliferation in human colorectal carcinoma tissue.","authors":"Liang-Liang Yu, Hong-Gang Yu, Jie-Ping Yu, He-Sheng Luo","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Activation of transcription factor nuclear factor-kappa B (NF-kappa B) has been shown to play a role in cell proliferation, apoptosis, cytokine production, and oncogenesis. The purpose of this study was to determine whether NF-kappa B is constitutively activated in human colorectal tumor tissues and, if so, to determine the role of NF-kappa B in colorectal tumorigenesis, furthermore, to determine the association of RelA expression with the expression of cyclooxygenase-2 (COX-2) and tumor cell proliferation.Methods: Paraffin sections of the normal epithelial, adenomatous and adenocarcinoma tissue were analysed immunohistochemically for RelA, COX-2, Ki-67 protein expression. EMSA (electrophoretic mobility shift assay) was used to confirm the increased nuclear translocation of RelA in colorectal tumor tissues. The expression of COX-2 mRNA was determined by RT-PCR (reverse transcription polymerase chain reaction) analysis.Results: Activation of NF-kappa B was significantly higher in adenocarcinoma tissue in comparison to that in adenomatous and normal epithelial tissue. The colon tumor cell proliferation, mRNA expression and protein level of COX-2 were significantly increased in the transition from normal to tumor tissue.Conclusion: Our results suggest that NF-kappa B may promote proliferation via enhancing the expression of COX-2, and the increased expression of RelA/nuclear factor-kappa B plays an important role in the pathogenesis of colorectal carcinoma.","PeriodicalId":77530,"journal":{"name":"Eksperimental'naia onkologiia","volume":"26 1","pages":"40-7"},"PeriodicalIF":0.0,"publicationDate":"2004-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24492073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different antitumor immunity roles of cytokine activated T lymphocytes from naive murine splenocytes and from dendritic cells-based vaccine primed splenocytes: implications for adoptive immunotherapy. 细胞因子激活的T淋巴细胞的抗肿瘤免疫作用不同，这些T淋巴细胞来自幼年小鼠脾细胞和基于树突状细胞疫苗的脾细胞:过继免疫治疗的意义

Eksperimental'naia onkologiia Pub Date : 2004-03-01

Sheng Zhang, Qin Wang, Wen-Feng Li, Hong-Yi Wang, Hong-Jun Zhang, Jing-Juan Zhu

{"title":"Different antitumor immunity roles of cytokine activated T lymphocytes from naive murine splenocytes and from dendritic cells-based vaccine primed splenocytes: implications for adoptive immunotherapy.","authors":"Sheng Zhang, Qin Wang, Wen-Feng Li, Hong-Yi Wang, Hong-Jun Zhang, Jing-Juan Zhu","doi":"","DOIUrl":"","url":null,"abstract":"Aim: The aim of the study is to explore the antitumor capacity of effector cells generated from murine splenocytes with sequential addition of a cocktail of cytokines and the possible contribution of dendritic cells to the antitumor capacity of these effector cells.Methods and results: Interferon-gamma, interleukin (IL)-1 beta, anti-CD3 mAb and IL-2 were used to activate murine splenocytes either from naive mice (termed cytokine activated T cells, CAT) or from DC based vaccine primed mice (termed specific effector T cells, SET). The antitumor roles of SET and CAT were analyzed in murine L615 T lymphocytic leukemia. Both CAT and SET were CD4(+)-predominant phenotypically and didn't show any significant cytotoxicity against a variety of syngeneic and allogeneic target cell lines using 51Cr release assay. When injected in vivo in combination with CY, CAT can cure a large proportion of leukemia mice. The cured mice couldn't establish specific antitumor immunity. However, in contrast to the roles of CAT, SET show far superior antitumor efficacy on a per cell basis compared with CAT. Moreover, the SET cured mice developed tumor specific long term memory immunity which was sufficient to reject a subsequent otherwise lethal tumor cells rechallenge and was transferable to naive immunocompetent mice.Conclusion: Our data demonstrate that there remain fundamentally different antitumor functions of CAT and SET which might be useful in the immunotherapy strategy choices.","PeriodicalId":77530,"journal":{"name":"Eksperimental'naia onkologiia","volume":"26 1","pages":"55-62"},"PeriodicalIF":0.0,"publicationDate":"2004-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24492567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0