Effects of two different high doses of irradiation on antioxidant system in the liver of guinea pigs.

Eksperimental'naia onkologiia Pub Date : 2004-03-01

Yildiz Guney, Neslihan Bukan, Aysen Dizman, Ayse Hicsonmez, Ayse Bilgihan

{"title":"Effects of two different high doses of irradiation on antioxidant system in the liver of guinea pigs.","authors":"Yildiz Guney, Neslihan Bukan, Aysen Dizman, Ayse Hicsonmez, Ayse Bilgihan","doi":"","DOIUrl":null,"url":null,"abstract":"Aim: To examine the state of the oxidant-antioxidant system in the liver of guinea pig caused by high doses of ionizing radiation in the early period.Methods: The research was carried out on guinea pigs irradiated with the doses of 8 Gy (group 2) or 15 Gy (group 3) (single dose/whole body) in comparison with control group (group 1). The levels of thiobarbituric acid reactive substances (TBARS) and glutathione (GSH), the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and the levels of selenium in the liver were measured.Results: TBARS levels in the irradiated animals were markedly higher than those in controls. In group 3, GSH levels and GSH-Px activity were significantly increased while activity of SOD and CAT were significantly decreased compared to groups 1 and 2. Liver selenium levels were not influenced by irradiation.Conclusion: The data have shown that gamma-irradiation at the doses of 8 Gy or 15 Gy results in significant increase in free radical formation while antioxidant enzymes were affected only at a dose of 15 Gy.","PeriodicalId":77530,"journal":{"name":"Eksperimental'naia onkologiia","volume":"26 1","pages":"71-4"},"PeriodicalIF":0.0000,"publicationDate":"2004-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Eksperimental'naia onkologiia","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Aim: To examine the state of the oxidant-antioxidant system in the liver of guinea pig caused by high doses of ionizing radiation in the early period.

Methods: The research was carried out on guinea pigs irradiated with the doses of 8 Gy (group 2) or 15 Gy (group 3) (single dose/whole body) in comparison with control group (group 1). The levels of thiobarbituric acid reactive substances (TBARS) and glutathione (GSH), the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and the levels of selenium in the liver were measured.

Results: TBARS levels in the irradiated animals were markedly higher than those in controls. In group 3, GSH levels and GSH-Px activity were significantly increased while activity of SOD and CAT were significantly decreased compared to groups 1 and 2. Liver selenium levels were not influenced by irradiation.

Conclusion: The data have shown that gamma-irradiation at the doses of 8 Gy or 15 Gy results in significant increase in free radical formation while antioxidant enzymes were affected only at a dose of 15 Gy.

本刊更多论文

两种不同高剂量辐照对豚鼠肝脏抗氧化系统的影响。

目的:探讨早期高剂量电离辐射对豚鼠肝脏氧化-抗氧化系统的影响。方法:采用8 Gy(2组)或15 Gy(3组)单剂量/全身照射豚鼠，与对照组(1组)比较，测定肝组织中硫代巴比妥酸活性物质(TBARS)和谷胱甘肽(GSH)水平、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH- px)活性及硒含量。结果:辐照动物TBARS水平明显高于对照组。与1、2组相比，3组GSH水平和GSH- px活性显著升高，SOD和CAT活性显著降低。肝脏硒水平不受辐照影响。结论:数据表明，8 Gy或15 Gy剂量的γ辐照可显著增加自由基的形成，而抗氧化酶仅在15 Gy剂量时受到影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Eksperimental'naia onkologiia

自引率

0.00%

发文量