American Journal of Tropical Medicine and Hygiene最新文献_第6页

Intravenous Fluid Bolus Resuscitation Increases Mortality Risk in Malawian Children with Cerebral Malaria. 静脉输液复苏增加马拉维脑型疟疾儿童的死亡风险。

IF 1.9 4区医学

American Journal of Tropical Medicine and Hygiene Pub Date : 2025-06-03 Print Date: 2025-07-02 DOI: 10.4269/ajtmh.24-0700

Meredith G Sherman, Pallavi Dwivedi, Ronke Olowojesiku, Rami Imam, Kennedy M Chastang, Eduardo A Trujillo-Rivera, James Bost, Amina M Mukadam, Nicole O'Brien, Christopher Alan Moxon, Alice M Liomba, Karl B Seydel, Douglas G Postels

{"title":"Intravenous Fluid Bolus Resuscitation Increases Mortality Risk in Malawian Children with Cerebral Malaria.","authors":"Meredith G Sherman, Pallavi Dwivedi, Ronke Olowojesiku, Rami Imam, Kennedy M Chastang, Eduardo A Trujillo-Rivera, James Bost, Amina M Mukadam, Nicole O'Brien, Christopher Alan Moxon, Alice M Liomba, Karl B Seydel, Douglas G Postels","doi":"10.4269/ajtmh.24-0700","DOIUrl":"10.4269/ajtmh.24-0700","url":null,"abstract":"The Fluid Expansion as Supportive Therapy (FEAST) clinical trial determined that African children with impaired perfusion receiving bolus intravenous (IV) fluids had increased mortality compared to children with impaired perfusion not receiving bolus IV fluids. Malaria was common in FEAST enrollees, but no stratified analysis for children with cerebral malaria (CM), a common cause of febrile coma in Africa, was reported. We investigated whether bolus fluid expansion changed mortality risk in children with CM. To evaluate this association, we performed a propensity score matched retrospective cohort study with data collected from 1,674 children with CM admitted to Queen Elizabeth Central Hospital in Blantyre, Malawi from 2000 to 2018. After matching of participants by covariate balancing propensity score weighting, children who received an IV fluid bolus had increased mortality risk (odds ratio [OR]: 1.92; 95% CI: 1.36-2.71) compared with those who did not. When stratified by admission systolic blood pressure (SBP), children with an SBP greater than 100 mm Hg receiving bolus fluids had increased mortality (OR: 3.15; 95% CI: 1.81-5.48) compared with those not receiving bolus fluids. In children with an SBP ≤100 mm Hg at admission, receiving bolus IV fluids did not change mortality (OR: 1.44; 95% CI: 0.91-2.26). Bolus IV fluids are an ineffective therapeutic intervention in children with CM and are harmful in those with normal or elevated admission SBPs. Our results confirm the lack of efficacy and potential harm of IV bolus fluid administration in Malawian children with CM.","PeriodicalId":7752,"journal":{"name":"American Journal of Tropical Medicine and Hygiene","volume":" ","pages":"42-48"},"PeriodicalIF":1.9,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12225554/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strongyloides stercoralis, but Not Hookworm, First-Stage Larvae Require Stool-Soluble Components for Growth and Development. 粪圆线虫，但不是钩虫，第一阶段幼虫需要粪溶性成分生长发育。

IF 1.9 4区医学

American Journal of Tropical Medicine and Hygiene Pub Date : 2025-05-27 DOI: 10.4269/ajtmh.25-0167

Patthanasak Khammaneechan, Prasit Na-Ek, Attarat Pattanawongsa, Blego Sedionoto, Witthaya Anamnart

{"title":"Strongyloides stercoralis, but Not Hookworm, First-Stage Larvae Require Stool-Soluble Components for Growth and Development.","authors":"Patthanasak Khammaneechan, Prasit Na-Ek, Attarat Pattanawongsa, Blego Sedionoto, Witthaya Anamnart","doi":"10.4269/ajtmh.25-0167","DOIUrl":"https://doi.org/10.4269/ajtmh.25-0167","url":null,"abstract":"In regions with prolonged rainy seasons where hookworm and Strongyloides stercoralis (S. stercoralis) are co-endemic, the prevalence of hookworm infection frequently exceeds that of strongyloidiasis, despite both parasites sharing a similar route of infection via skin penetration by third-stage larvae (L3). Climate factors may contribute because rainwater can dilute stool, reducing bacterial abundance and stool-soluble component concentrations that may affect first-stage larvae (L1) development. In this study, we aimed to compare the stool-soluble component requirements of S. stercoralis and hookworm L1 in water-diluted stool and soil. Four sets of stool suspensions at 1:20, 1:480, and 1:30,720 dilutions, each containing 30-40 hookworm or S. stercoralis L1, were prepared. Two sets were adjusted to a minimal volume for soil addition, with and without bacteria, while the other two remained unadjusted. All suspensions were incubated at room temperature for 6 days, and the L3 were counted. In 1:20 suspensions, both hookworm and S. stercoralis L1 developed into or produced L3. However, in 1:480 suspensions, only hookworm L1 progressed to L3, regardless of bacterial addition. Neither species developed into L3 in 1:30,720 suspensions without bacteria, although hookworm L1 successfully developed into L3 when bacteria were added. Additionally, hookworm L1 developed into L3 in soil, whereas S. stercoralis L1 did not. These findings indicate that hookworm L1 require only bacteria, whereas S. stercoralis L1 depend on both bacteria and sufficient stool-soluble components. Water-diluting stool suppresses S. stercoralis L1 growth and development but not hookworm L1, which explains the higher prevalence of hookworm infection in regions with long rainy seasons.","PeriodicalId":7752,"journal":{"name":"American Journal of Tropical Medicine and Hygiene","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paederus Dermatitis Acquired in the Gambia. 冈比亚获得性拟足性皮炎。

IF 1.9 4区医学

American Journal of Tropical Medicine and Hygiene Pub Date : 2025-05-27 DOI: 10.4269/ajtmh.25-0071

Olabisi Ogunbiyi, Natasha M Mawani, Stephen L Walker

引用次数: 0

Pulmonary Paragonimiasis: A Pitfall in IgG4-Related Disease Diagnosis. 肺吸虫病：igg4相关疾病诊断中的一个陷阱。

IF 1.9 4区医学

American Journal of Tropical Medicine and Hygiene Pub Date : 2025-05-27 DOI: 10.4269/ajtmh.25-0112

Hidenori Takahashi, Takumi Yasuda, Mio Kokubo-Tanaka

引用次数: 0

Comparison between Saliva and Nasopharyngeal Swabs for the Rapid Detection of Respiratory Viruses Using the Roche Cobas® Liat® Polymerase Chain Reaction System in Rural Guatemala. 用罗氏Cobas®Liat®聚合酶链反应系统快速检测危地马拉农村呼吸道病毒的唾液和鼻咽拭子的比较

IF 1.9 4区医学

American Journal of Tropical Medicine and Hygiene Pub Date : 2025-05-27 DOI: 10.4269/ajtmh.24-0184

Claudia Paiz-Reyes, Kareen Arias, Julio Del Cid-Villatoro, Daniel Vásquez, Melissa Gomez, Neudy Rojop, Wanda Mejia, Molly M Lamb, May Chu, Diva M Calvimontes Barrientos, Edwin J Asturias, Daniel Olson

{"title":"Comparison between Saliva and Nasopharyngeal Swabs for the Rapid Detection of Respiratory Viruses Using the Roche Cobas® Liat® Polymerase Chain Reaction System in Rural Guatemala.","authors":"Claudia Paiz-Reyes, Kareen Arias, Julio Del Cid-Villatoro, Daniel Vásquez, Melissa Gomez, Neudy Rojop, Wanda Mejia, Molly M Lamb, May Chu, Diva M Calvimontes Barrientos, Edwin J Asturias, Daniel Olson","doi":"10.4269/ajtmh.24-0184","DOIUrl":"https://doi.org/10.4269/ajtmh.24-0184","url":null,"abstract":"We evaluated the performance of saliva compared with nasopharyngeal (NP) swabs for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A/B, and respiratory syncytial virus (RSV) using the Roche cobas® Liat® point-of-care platform (Roche, Basel, Switzerland). Within an existing adult cohort in Guatemala, consenting participants with influenza-like illness (ILI) provided paired saliva and NP swabs, which were tested for SARS-CoV-2, influenza A/B, and RSV on the Liat platform. Among 138 ILI episodes from 123 enrolled subjects, 35/124 (28.2%), 5/135 (3.7%), 6/135 (4.4%), and 7/135 (5.2%) samples were reverse transcription polymerase chain reaction-positive for SARS-CoV-2, influenza A, influenza B, and RSV, respectively. For SARS-CoV-2, saliva/NP agreement was 90.3% (Cohen's kappa = 0.731); 12 (9.7%) of the samples were discordant (three saliva-positive only, nine NP-positive only); and the median cycle threshold (Ct) for saliva was higher than that for NP (26.53 versus 23.54; P = 0.005). In our setting, saliva had a lower viral load correlate (Ct) but maintained a good correlation with NP swabs for the detection of SARS-CoV-2 on the Liat platform.","PeriodicalId":7752,"journal":{"name":"American Journal of Tropical Medicine and Hygiene","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correct Knowledge about Tuberculosis and Determinants among People Living with HIV Attending a District Antiretroviral Treatment Center in the State of Karnataka, India. 印度卡纳塔克邦地区抗逆转录病毒治疗中心艾滋病毒感染者对结核病和决定因素的正确认识。

IF 1.9 4区医学

American Journal of Tropical Medicine and Hygiene Pub Date : 2025-05-27 DOI: 10.4269/ajtmh.24-0600

Jeni Jacob, Ankeeta Menona Jacob, Avinash K Shetty

{"title":"Correct Knowledge about Tuberculosis and Determinants among People Living with HIV Attending a District Antiretroviral Treatment Center in the State of Karnataka, India.","authors":"Jeni Jacob, Ankeeta Menona Jacob, Avinash K Shetty","doi":"10.4269/ajtmh.24-0600","DOIUrl":"https://doi.org/10.4269/ajtmh.24-0600","url":null,"abstract":"Tuberculosis (TB) is a major global public health concern. People living with HIV (PLHIV) are at an increased risk of developing active TB, with a 20-fold higher risk compared with those without HIV infection. Gaps in TB knowledge among PLHIV patients have been linked to delays in the diagnosis of TB and poor adherence to TB medications, resulting in substantial morbidity and mortality. In the current study, the correct knowledge of TB and its determinants among PLHIV patients attending a district antiretroviral therapy (ART) center in Karnataka, India, was assessed. A cross-sectional study was conducted at a district ART facility in Karnataka between October 2022 and April 2023. A pretested questionnaire was used to gather data, and SPSS version 29.0 (IBM Corp., Armonk, NY) was used for analysis. The proportion of PLHIV with correct TB knowledge and sources was expressed in percentages. Associations between correct TB knowledge and sociodemographic variables were assessed using χ2 and Fisher's tests. Among 432 participants, 222 (51.4%) were male, and 191 (44.1%) had education beyond high school. Correct TB knowledge was found in 253 (58.5%) participants and was significantly associated with educational status, monthly family income, source of TB information, TB preventive treatment intake, and public attitude stigma in multivariate analysis. Healthcare workers were the primary source of correct TB knowledge for 388 (97.4%) participants. This study highlights the need to strengthen TB education and awareness among PLHIV to improve their health-seeking behavior and facilitate early TB detection.","PeriodicalId":7752,"journal":{"name":"American Journal of Tropical Medicine and Hygiene","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing Molecular Techniques to Inform Species-Specific Hookworm Prevalence and Infection Intensity and Detect Strongyloides in Dak Lak Province, Vietnam. 利用分子技术了解越南达克湖省特定种类钩虫流行率和感染强度并检测类圆线虫。

IF 1.9 4区医学

American Journal of Tropical Medicine and Hygiene Pub Date : 2025-05-27 DOI: 10.4269/ajtmh.24-0670

Wenyu Liu, Adam W Bartlett, Katrina Blazek, Sze Fui Hii, Clare E F Dyer, Vito Colella, Rebecca J Traub, Dinh Ng-Nguyen, Susana Vaz Nery

{"title":"Harnessing Molecular Techniques to Inform Species-Specific Hookworm Prevalence and Infection Intensity and Detect Strongyloides in Dak Lak Province, Vietnam.","authors":"Wenyu Liu, Adam W Bartlett, Katrina Blazek, Sze Fui Hii, Clare E F Dyer, Vito Colella, Rebecca J Traub, Dinh Ng-Nguyen, Susana Vaz Nery","doi":"10.4269/ajtmh.24-0670","DOIUrl":"https://doi.org/10.4269/ajtmh.24-0670","url":null,"abstract":"Quantitative polymerase chain reaction (qPCR) is an increasingly recognized diagnostic tool for soil-transmitted helminths (STHs), overcoming limitations of microscopy. We compared qPCR with Kato-Katz (KK) and sodium nitrate flotation (SNF; specific gravities 1.2 and 1.3) in determining STH prevalence and infection intensity using samples from a community survey of 351 people in Dak Lak province, Vietnam. Established correlation formulas were used to convert qPCR cycle threshold values to eggs per gram to compare infection intensity. Sensitivity, specificity, and agreement between methods were also investigated. Hookworm was the predominant STH detected, with prevalences of 70.3% (95% CI: 63.6-76.9) by qPCR, 59.1% (95% CI: 46.3-72.4) by 1.2SNF, 56.5% (95% CI: 43.2-69.4) by 1.3SNF, and 47.8% (95% CI: 34.6-57.6) by KK. Detection of Ascaris lumbricoides and Trichuris trichiura was low across methods. Prevalences of zoonotic hookworm Ancylostoma ceylanicum and Strongyloides species by qPCR were 3.0% (95% CI: 1.3-6.4) and 17.3% (95% CI: 8.1-22.3), respectively. qPCR detected higher prevalence of moderate- (8.3% versus <2%) and heavy-intensity (9.4% versus 0%) infections than microscopy. The sensitivities of microscopy methods were 81.0% (1.2SNF), 75.8% (1.3SNF), and 67.4% (KK). Diagnostic agreement for hookworm was moderate between qPCR and KK and between qPCR and 1.3SNF, and it was good between qPCR and 1.2SNF. The superior performance of qPCR in detecting hookworm coupled with the capacity to differentiate hookworm species and detect Strongyloides spp. supports the role of qPCR to inform control strategies, particularly for zoonotic reservoirs and use of macrocyclic lactones.","PeriodicalId":7752,"journal":{"name":"American Journal of Tropical Medicine and Hygiene","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proactive Tethering to Prevent Guinea Worm Infections among Dogs in Chad: An Analysis of the Impacts of Timing and Dog Selection. 主动拴绳预防乍得狗的麦地那龙线虫感染：时间和狗选择的影响分析。

IF 1.9 4区医学

American Journal of Tropical Medicine and Hygiene Pub Date : 2025-05-27 DOI: 10.4269/ajtmh.24-0673

Hannah Smalley, Pinar Keskinocak, Julie Swann, Maryann G Delea, Obiora A Eneanya, Adam Weiss

{"title":"Proactive Tethering to Prevent Guinea Worm Infections among Dogs in Chad: An Analysis of the Impacts of Timing and Dog Selection.","authors":"Hannah Smalley, Pinar Keskinocak, Julie Swann, Maryann G Delea, Obiora A Eneanya, Adam Weiss","doi":"10.4269/ajtmh.24-0673","DOIUrl":"https://doi.org/10.4269/ajtmh.24-0673","url":null,"abstract":"Guinea worm, or dracunculiasis, has been targeted for eradication. Since the detection of the disease in animals, particularly domestic dogs in Chad, eradication efforts have become more challenging as animal infections perpetuate the cycle of infection and continue to put humans at risk. A wide range of community-based interventions is used to reduce infections, and the proactive tethering of dogs was introduced in 2020 to aid in the eradication efforts. This approach aims to contain dogs to prevent them from infecting water sources and to minimize exposure to water sources harboring infective Guinea worm larvae. However, tethering guidelines are not uniformly adhered to across communities. We adapted an agent-based simulation model to analyze various proactive tethering scenarios that consider dog selection methods, time of year, and time of day. Tethering 100% of dogs year-round but releasing them for part of the day (e.g., at night) results in 19-24% more infections compared with the full-day tethering of 60% of dogs year-round. Tethering 80% of dogs during the 3 months of peak infectivity, from April to June, results in 5-9% fewer infections than tethering 100% of dogs for 6 months during the dry season, from November to April. Because of the protracted prepatent period of Guinea worm disease (∼10-14 months), the results of interventions are not known until 1 year after they are implemented. Understanding the long-term impacts of tethering decisions when they are made is extremely important for achieving the ultimate goal of Guinea worm eradication.","PeriodicalId":7752,"journal":{"name":"American Journal of Tropical Medicine and Hygiene","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a Lassa Fever Specimen Biobank in Nigeria. 在尼日利亚建立拉沙热标本生物库。

IF 1.9 4区医学

American Journal of Tropical Medicine and Hygiene Pub Date : 2025-05-27 DOI: 10.4269/ajtmh.24-0527

Hanesh F Chi, Johnson Etafo, Fritz Fonkeng, Gbenga-Ayeni Bosede Olufunke, Ronke Ireneh, Chukwuyem Abejegah, Sampson Owhin, Stella Somiari, Aurélia Vessière, Daniel G Bausch, Imane El Idrissi, Warren Fransman, Cassandra Kelly-Cirino, Emmanuel Agogo, Devy M Emperador, Nelson A Adedosu

{"title":"Establishment of a Lassa Fever Specimen Biobank in Nigeria.","authors":"Hanesh F Chi, Johnson Etafo, Fritz Fonkeng, Gbenga-Ayeni Bosede Olufunke, Ronke Ireneh, Chukwuyem Abejegah, Sampson Owhin, Stella Somiari, Aurélia Vessière, Daniel G Bausch, Imane El Idrissi, Warren Fransman, Cassandra Kelly-Cirino, Emmanuel Agogo, Devy M Emperador, Nelson A Adedosu","doi":"10.4269/ajtmh.24-0527","DOIUrl":"https://doi.org/10.4269/ajtmh.24-0527","url":null,"abstract":"Despite being a dangerous disease found across West Africa, with occasional cases imported elsewhere, few medical countermeasures for Lassa fever are available, with no vaccine or validated treatment, and limited regulatory-approved diagnostics. Scientific research on the Lassa virus (LASV) is needed to accelerate the development of such tools but is dependent on access to high-quality biological samples. To meet this need, we have established a well-curated biobank to prospectively collect, process, and store high-quality, well-characterized clinical specimens from patients with Lassa fever at the Federal Medical Center Owo (FMCO) in Nigeria. Patients presenting to FMCO with symptoms of Lassa fever were tested for LASV by polymerase chain reaction and screened for eligibility. Samples of whole blood, plasma, and serum were collected at baseline, and at 4- and 8-weeks post-baseline from LASV-positive (LASV+) participants. Of 197 participants enrolled, 99 were LASV+ and 98 LASV-negative. In total, 3,599 sample aliquots were stored, comprised of 264 whole blood, 1,572 serum, and 1,763 plasma samples. The Lassa fever biobank now provides support for scientific research, including the evaluation of diagnostics and biomarkers, as well as the development and validation of other medical countermeasures for the detection, prevention, and control of Lassa fever. Processes for accessing samples are described.","PeriodicalId":7752,"journal":{"name":"American Journal of Tropical Medicine and Hygiene","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Dengue and Chikungunya Viruses in Mosquitoes from Nepal. 尼泊尔蚊子中登革热和基孔肯雅病毒的检测。

IF 1.9 4区医学

American Journal of Tropical Medicine and Hygiene Pub Date : 2025-05-27 DOI: 10.4269/ajtmh.24-0685

Maurine Mumo Mutua, Sandesh Rimal, Sabin Shrestha, Anjana Kharbuja, Anjali Dulal, Suprabha Subedi, Renu Bhandari Dumre, Ashma Khadka, Ishan Gautam, Himal Luitel, Merveille Kapandji, Shingo Inoue, Yuki Takamatsu, Basu Dev Pandey, Satoshi Kaneko, Takeshi Urano, Kouichi Morita, Shyam Prakash Dumre, Mya Myat Ngwe Tun

{"title":"Detection of Dengue and Chikungunya Viruses in Mosquitoes from Nepal.","authors":"Maurine Mumo Mutua, Sandesh Rimal, Sabin Shrestha, Anjana Kharbuja, Anjali Dulal, Suprabha Subedi, Renu Bhandari Dumre, Ashma Khadka, Ishan Gautam, Himal Luitel, Merveille Kapandji, Shingo Inoue, Yuki Takamatsu, Basu Dev Pandey, Satoshi Kaneko, Takeshi Urano, Kouichi Morita, Shyam Prakash Dumre, Mya Myat Ngwe Tun","doi":"10.4269/ajtmh.24-0685","DOIUrl":"https://doi.org/10.4269/ajtmh.24-0685","url":null,"abstract":"The dengue virus (DENV) and chikungunya virus (CHIKV) infection present with similar symptoms and are transmitted by Aedes mosquitoes. Until now, there have been no reported cases of DENV and CHIKV being detected in mosquitoes in Nepal. This study identified circulating arboviruses from mosquitoes collected in the capital city Kathmandu, and Dhading, Bhaktapur, and Lalitpur districts of Central Nepal in 2023. Of 105 Aedes pooled samples, 16 (15.2%) were positive for DENV, and six (5.7%) were positive for CHIKV by quantitative real-time polymerase chain reaction. DENV-2 was the most common serotype (11.4%), aligning with recent outbreaks in Nepal in 2023, followed by DENV-3 and DENV-4 (1.9% each). To the best of our knowledge, our study is the first report to detect DENV and CHIKV in Aedes mosquitoes circulating in Nepal. This study highlights the need for mosquito surveillance to prevent future outbreaks of mosquito-borne diseases in Nepal.","PeriodicalId":7752,"journal":{"name":"American Journal of Tropical Medicine and Hygiene","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0