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Free-radical generation by monocytes and neutrophils: a possible cause of plasma lipoprotein modification. 由单核细胞和中性粒细胞产生自由基:血浆脂蛋白改变的可能原因。
Biomedical science Pub Date : 1991-01-01
O M Panasenko, T V Vol'nova, A N Osipov, O A Azizova, Vladimirov YuA
{"title":"Free-radical generation by monocytes and neutrophils: a possible cause of plasma lipoprotein modification.","authors":"O M Panasenko,&nbsp;T V Vol'nova,&nbsp;A N Osipov,&nbsp;O A Azizova,&nbsp;Vladimirov YuA","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The activation of freshly isolated human blood monocytes and neutrophils monitored by oxidized human plasma lipoproteins (LP) was measured by detecting luminol-amplified chemiluminescence. The activation was accompanied by production of superoxide radicals. This finding was confirmed by measuring superoxide dismutase-sensitive reduction of cytochrome c. Incubation of monocytes or neutrophils with low-density lipoproteins (LDL) resulted in the accumulation of lipid peroxidation (LPO) products which were assayed by the 2-thiobarbituric acid test. Data from inhibitory analysis suggest that the hydroxyl radical scavenger, mannitol, had no appreciable effect on the accumulation of LPO products during the incubation of LDL with either cell type. However, catalase, superoxide dismutase, the metal ion chelators desferrioxamine and EDTA, as well as the free radical scavenger, butylated hydroxytoluene, markedly decreased the accumulation of LPO products in the medium--by 88%, 67%, 38%, 52%, and 47%, respectively, after incubation of LDL with monocytes, and by 65%, 47%, 41%, 65%, and 100% after incubation of LDL with neutrophils. These results indicate that activation of monocytes and neutrophils by oxidized LP intensifies LPO which proceeds via a free-radical mechanism that is superoxide-dependent and is catalyzed by transition metals.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":"2 6","pages":"581-9"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13002794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification and characterization of a thymocyte growth factor. 1. Purification. 胸腺细胞生长因子的纯化及特性研究。1. 净化。
Biomedical science Pub Date : 1991-01-01
Talaev VYu, T M Shuvaeva, V M Lipkin, A A Yarilin, V P Shichkin
{"title":"Purification and characterization of a thymocyte growth factor. 1. Purification.","authors":"Talaev VYu,&nbsp;T M Shuvaeva,&nbsp;V M Lipkin,&nbsp;A A Yarilin,&nbsp;V P Shichkin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A thymocyte growth factor has been purified by gel filtration and reverse-phase hplc from the culture medium of a T-lymphocyte-precursor cell line TC.SC-1/2.0. The purified thymocyte growth factor possesses high growth-stimulating activity and has a relative molecular mass of 20,000, as determined by SDS-PAGE.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":"2 5","pages":"511-4"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13003029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transformation of rat-embryo immortalized fibroblasts by the E6-E7 region of human papillomavirus type 18. 18型人乳头瘤病毒E6-E7区转染大鼠胚胎永活成纤维细胞的研究。
Biomedical science Pub Date : 1991-01-01
E V Komissarova, D D Spitkovsky, F L Kisseljov
{"title":"Transformation of rat-embryo immortalized fibroblasts by the E6-E7 region of human papillomavirus type 18.","authors":"E V Komissarova,&nbsp;D D Spitkovsky,&nbsp;F L Kisseljov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Plasmids containing the E6 and E7 open reading frames of human papillomavirus type 18 transformed rat-embryo fibroblasts when expressed under the cytomegalovirus promoter. The fibroblasts had been previously immortalized with the large T-antigen gene of the polyomavirus to produce rat embryo fibroblast (large T-antigen) [REF(LT)] cells. REF(LT) cells were transformed by the E6 and E7 sequences to anchorage independence and tumourigenicity, but there were no significant morphological alterations. Transformation by these sequences of REF(LT) cells differed from that achieved by pEJras, in which case significant morphological changes and tumourigenicity in nude mice did occur.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":"2 3","pages":"305-8"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12826781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The possible involvement of glucocorticoids in the development of genetically determined autoimmune pathology in NZB mice. 糖皮质激素在NZB小鼠遗传决定的自身免疫病理发展中的可能参与。
Biomedical science Pub Date : 1991-01-01
V M Chesnokova, V L Chukhlib, E V Ignat'eva, L N Ivanova
{"title":"The possible involvement of glucocorticoids in the development of genetically determined autoimmune pathology in NZB mice.","authors":"V M Chesnokova,&nbsp;V L Chukhlib,&nbsp;E V Ignat'eva,&nbsp;L N Ivanova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The total blood corticosterone concentration, the reactivity of the adrenal glands to exogenous adrenocorticotrophic hormone, and the binding capacity of corticosteroid-binding globulin (CBG), were determined in male NZB mice that spontaneously develop an autoimmune disease. The appearance of antibodies to endogenous erythrocytes was also monitored. This study was performed on animals of the following age groups: (1) 2-3-month-old animals prior to the appearance of autoantibodies; (2) 6-7-month-old animals in the period when the disease developed; and (3) 10-12-month-old animals, i.e. the period when the autoimmune disease developed. It was demonstrated that in the 10-12-month-old mice there was a sharp decrease in the total corticosterone level in the plasma (from 152 +/- 32 to about 20 micrograms l-1), and the reactivity of the adrenal glands to exogenous corticotrophin decreased (from 400% to 120%). However, the binding capacity of CBG increased with age which led to a decrease in the pool of active corticosterone in the blood. The increase in this capacity was especially pronounced in affected 10-12-month-old animals. (The number of binding sites increased from 0.64 +/- 0.05 to 0.8 +/- 0.03 microM and the values of the association constant for the binding of corticosterone to CBG increased from 0.29 +/- 0.05 to 0.91 +/- 0.07 microM-1). The combination of the above-mentioned changes suggests that there is a direct correlation between the decrease in the glucocorticoid background in the NZB mice and in the appearance of autoantibodies.(ABSTRACT TRUNCATED AT 250 WORDS)</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":"2 6","pages":"557-61"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12834265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interhemispheric asymmetry of EEG coherence as a reflection of different functional states of the human brain. 脑电相干性的半球间不对称是人脑不同功能状态的反映。
Biomedical science Pub Date : 1991-01-01
G N Boldyreva, L A Zhavoronkova
{"title":"Interhemispheric asymmetry of EEG coherence as a reflection of different functional states of the human brain.","authors":"G N Boldyreva,&nbsp;L A Zhavoronkova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>EEG coherence analysis was used to study aspects of the synchronisation of electrical processes in the left and right cerebral hemispheres of right-handed and left-handed healthy subjects in a state of calm wakefulness. Right-handed subjects showed a greater coherence in the left hemisphere and left-handed subjects in the right hemisphere. There were also differences between right-handed and left-handed subjects in the regional profiles of interhemispheric asymmetry and in the interhemispheric asymmetry of individual spectral bands. These differences may reflect variations in the involvement of cortical and subcortical cerebral structures in the formation of the hemispheric specificity. Changes in interhemispheric coherence with the onset of drowsiness were also observed. This suggests that changes in the interconnections of neural networks are also involved in changes in arousal. Certain characteristic regional interhemispheric asymmetry patterns and asymmetries in the spectral bands appear to be necessary for normal human brain function.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":"2 3","pages":"266-70"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12914624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HIV-1 gag proteins in virions and in infected cell fractions. 病毒粒子和感染细胞片段中的HIV-1 gag蛋白。
Biomedical science Pub Date : 1991-01-01
N K Sharova, V B Grigor'ev, A G Bukrinskaya
{"title":"HIV-1 gag proteins in virions and in infected cell fractions.","authors":"N K Sharova,&nbsp;V B Grigor'ev,&nbsp;A G Bukrinskaya","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The relation of the initial products of the HIV-1 gag gene to the final products was determined in virus samples and cell fractions of infected H9 and Jurkat-tat cell cultures. The proteins were identified by immunoblotting with pooled sera from AIDS patients or monoclonal antibodies. The proportion in the virions of gag precursor proteins and the products of their proteolytic cleavage varied according to the maturity of the virus particles as determined by electron microscopy. The distribution of viral gag proteins in the cell fractions was determined 2, 4, and 24 h after infection. Treatment of cells with cycloheximide to block de novo protein synthesis did not significantly affect the results. Gag proteins containing the N terminus of the precursor p55 (including p55, the intermediate precursors p41(45) and p39, and mature protein p17) were found in the cell nuclei up to 24 h after infection. The major core protein p24 was located in the cytoplasmic fraction. These data strongly suggest that gag precursors from the p55 N terminus and the matrix protein p17 enter the infected cell separately from the major core protein p24, or become separated from it in the cytoplasm.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":"2 3","pages":"279-84"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12914626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Karyological approach to the identification of true cell lines susceptible to the human immunodeficiency virus (HIV). 鉴定对人类免疫缺陷病毒(HIV)敏感的真细胞系的核生物学方法。
Biomedical science Pub Date : 1991-01-01
L A Glukhova, I A Kiseleva, M N Korneeva, D N Nosik, A A Kushch, S E Mamaeva, B Ashe
{"title":"Karyological approach to the identification of true cell lines susceptible to the human immunodeficiency virus (HIV).","authors":"L A Glukhova,&nbsp;I A Kiseleva,&nbsp;M N Korneeva,&nbsp;D N Nosik,&nbsp;A A Kushch,&nbsp;S E Mamaeva,&nbsp;B Ashe","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A karyological analysis of twenty-two variants of eight cell lines, which differed in their susceptibility to the human immunodeficiency virus (HIV) and which had been obtained from different sources, was carried out by means of differential chromosome staining for G and C bands. The karyotypes of eight T-lymphoblastoid cell lines were identified, including five (MT-4, Molt-3, CEM, H-9, and Hut-78) not previously studied by cytogenetic methods. Karyotyping confirmed the identity of seventeen variants of the eight cell lines, and five variants of four lines were found to be misidentified. Comparative analysis of the cytogenetic characteristics of the three CEM-line variants demonstrates the need for karyotype evaluation in the course of in vitro cell cultivation. Fourteen identical marker chromosomes were revealed in H-9 and Hut-78 cell karyotypes, confirming the common origin of these two lines. It was found that the cells of the HIV-susceptible lines had a tendency to undergo polyploidisation both during the initial stages after isolation and in the course of cultivation.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":"2 3","pages":"293-7"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12914628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunopotentiometric electrodes based on bioelectrocatalysis in the absence of mediators. 在没有介质的情况下，基于生物电催化的免疫电位电极。
Biomedical science Pub Date : 1991-01-01
A L Ghindilis, O V Skorobogat'ko, A I Yaropolov
{"title":"Immunopotentiometric electrodes based on bioelectrocatalysis in the absence of mediators.","authors":"A L Ghindilis,&nbsp;O V Skorobogat'ko,&nbsp;A I Yaropolov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A new method of immunoelectrochemical analysis employing laccase as the enzyme label is described. The ability of the enzyme to catalyze electroreduction of oxygen via a direct mechanism allows the detection of the biospecific interaction of a laccase-labeled receptor, or antibody, with a ligand-modified electrode. Formation of a complex between the laccase-labeled antibody and the antigen on the electrode surface resulted in a considerable (greater than 300 mV) change in the electrode potential. Analysis was performed in a competitive scheme, and a single measurement could be made within 20 min in the absence of an electrochemically active mediator. The reaction substrates were atmospheric oxygen and electrons that were transferred directly from the electrode to the active site of the enzyme label. The use of a composite carbon material containing a polyethyleneimine-based polymer eliminated nonspecific interactions between the reaction components and the electrode surface. Insulin and mouse immunoglobulin were used as a model analytes.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":"2 5","pages":"520-2"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13002153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of costal cartilage collagen in funnel chest. 漏斗胸肋软骨胶原蛋白的特征。
Biomedical science Pub Date : 1991-01-01
N N Prozorovskaya, E A Kozlov, A V Voronov, V A Verovskii, A A Delvig
{"title":"Characterization of costal cartilage collagen in funnel chest.","authors":"N N Prozorovskaya,&nbsp;E A Kozlov,&nbsp;A V Voronov,&nbsp;V A Verovskii,&nbsp;A A Delvig","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Parallel measurements of pyridinoline content, the ratio of soluble:insoluble collagen, and the percentage of endogenous collagenolysis were made in samples of costal cartilage from forty-five children with funnel chest (FC) and from twenty-two control children. From an analysis of the influence of different factors, such as FC type (isolated or syndromic), a diagnosed syndrome, extent of FC depression (second or third), and age, two biochemical variants of FC-variants a and b, which were not related to the presence or absence of a known concurrent syndrome, were distinguished. These variants differed from each other in all the parameters under study. Variant a occurred about five times less frequently than b, and was characterized by a pyridinoline content of about 50% of that of b, an elevated soluble:insoluble collagen ratio, and an increased percentage of endogenous collagenolysis compared to controls. For variant b, the pyridinoline content fell within normal limits, but the soluble:insoluble collagen ratio, and the percentage of endogenous collagenolysis were below normal. The data suggest that the formation of variant a may be related to defect(s) in collagen crosslinking, whereas the formation of variant b may result from other unknown factor(s) involved in the formation and maturation of costal cartilage.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":"2 6","pages":"576-80"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13002889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polymerase chain reaction polymorphisms in HLA-DQ alpha and IL6 from Mongoloid and Caucasoid populations. 蒙古人种和高加索人种HLA-DQ α和il - 6的聚合酶链反应多态性
Biomedical science Pub Date : 1991-01-01
N V Titenko, J H Kurth, A M Bowcock, L L Cavalli-Sforza
{"title":"Polymerase chain reaction polymorphisms in HLA-DQ alpha and IL6 from Mongoloid and Caucasoid populations.","authors":"N V Titenko,&nbsp;J H Kurth,&nbsp;A M Bowcock,&nbsp;L L Cavalli-Sforza","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Polymerase chain reaction polymorphisms have been used to study European, Chinese, Russian and Buryat populations at the HLA-DQ alpha and IL6 loci. DNA from individuals in these populations was specifically amplified at these loci, and analyzed either with allele-specific oligonucleotides for HLA-DQ alpha or directly on agarose gels for IL6. Allelic frequencies were calculated for each of the loci in each population. Comparisons between the population frequencies show that the Russian population is more closely related to Europeans, and that the Buryat population is more closely related to Chinese. This finding is in agreement with conclusions based on the phenotypic frequencies of classical blood-group and other protein markers in these populations.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":"2 2","pages":"175-9"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12935362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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