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Expression of human membrane proteins 4.1a and 4.1b in reticulocytes and mature erythrocytes. 网状红细胞和成熟红细胞中人膜蛋白4.1a和4.1b的表达。
Biomedical science Pub Date : 1991-01-01
T P Molchanova, S V Kolodei, Tokarev YuN
{"title":"Expression of human membrane proteins 4.1a and 4.1b in reticulocytes and mature erythrocytes.","authors":"T P Molchanova,&nbsp;S V Kolodei,&nbsp;Tokarev YuN","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The erythrocyte membrane protein of band 4.1 is an essential protein of the erythrocyte cytoskeleton. Two isoforms have been detected in human erythrocytes, and six in nucleated avian erythrocytes. Multiple alternative splicing of human reticulocyte mRNA for protein 4.1 implies the possibility of even more isoforms. Structural and functional dissimilarities between isoforms, and their differential expression during the course of erythropoiesis are not well understood. Here, we show that only protein 4.1b is present in the immature reticulocytes of anaemic patients. The ratio of 4.1a to 4.1b in mature reticulocytes of healthy individuals was 1.25. This ratio was 2.0 in healty mature erythrocytes, and 0.7 in peripheral red blood cells from anaemic patients. Taking into account these and other available data, we speculate that the steady state levels of protein 4.1 isoforms change gradually in the terminal stages of erythropoiesis.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12831072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The mechanism of the modifying effect of ATP on Na(+)-K+ ATPase. ATP对Na(+)-K+ ATP酶的修饰作用机制。
Biomedical science Pub Date : 1991-01-01
A A Boldyrev, N U Fedosova, O D Lopina
{"title":"The mechanism of the modifying effect of ATP on Na(+)-K+ ATPase.","authors":"A A Boldyrev,&nbsp;N U Fedosova,&nbsp;O D Lopina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>On the basis of a review of the literature and a study of the molecular and kinetic properties of Na(+)-K+ ATPase, a model is proposed that explains the regulation of the activity of the enzyme by ATP in terms of an acceleration of the E2----E1 transition. It is presumed that the transition occurs via a short-lived oligomer whose formation is accelerated by ATP. In the context of this model, the non-Michaelis-Menton kinetics of the enzyme can be explained by interprotomer interactions. After solubilization of the enzyme with octaethylene glycol dodecyl ether, the hydrolysis of ATP follows ordinary Michaelis-Menton kinetics. The validity of the model is also supported by radiation-inactivation experiments with a nucleotide (GTP) which does not accelerate the E2----E1 transition, as well as by experiments with a low concentration of ATP. In both situations, the size of the molecular target corresponds to the monomeric form of the enzyme.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12834257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Migration of promoter elements between genes: a role in transcriptional regulation and evolution. 启动子元件在基因间的迁移:在转录调控和进化中的作用。
Biomedical science Pub Date : 1991-01-01
A Surguchov
{"title":"Migration of promoter elements between genes: a role in transcriptional regulation and evolution.","authors":"A Surguchov","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12846033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibition of contractile activity of rat cardiomyocytes in culture by alpha- and gamma-endorphins. 内啡肽和-内啡肽对培养大鼠心肌细胞收缩活性的抑制作用。
Biomedical science Pub Date : 1991-01-01
N A Sokolova, N L Ivanova, M I Leikina, I P Ashmarin
{"title":"Inhibition of contractile activity of rat cardiomyocytes in culture by alpha- and gamma-endorphins.","authors":"N A Sokolova,&nbsp;N L Ivanova,&nbsp;M I Leikina,&nbsp;I P Ashmarin","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12886566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of antigenic structure of attenuated and virulent Venezuelan equine encephalomyelitis virus by means of monoclonal antibodies. 用单克隆抗体研究委内瑞拉马脑脊髓炎减毒病毒的抗原结构。
Biomedical science Pub Date : 1991-01-01
I A Razumov, E V Agapov, A V Pereboev, E V Protopopova, S D Lebedeva, V B Loktev
{"title":"Investigation of antigenic structure of attenuated and virulent Venezuelan equine encephalomyelitis virus by means of monoclonal antibodies.","authors":"I A Razumov,&nbsp;E V Agapov,&nbsp;A V Pereboev,&nbsp;E V Protopopova,&nbsp;S D Lebedeva,&nbsp;V B Loktev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A comparative study of the antigenic structure of virulent strains and attenuated vaccine strains of Venezuelan equine encephalomyelitis virus (VEEV) by means of monoclonal antibodies has made it possible to investigate the antigenic structure of the envelope glycoproteins E1 and E2, and to specify their role in the development of antiviral immunity. On the E1 glycoprotein there are five nonoverlapping antigenic sites consisting of eight epitopes that are recognized by monoclonal antibodies; six sites consisting of twenty epitopes were found on the E2 glycoprotein. The monoclonal antibodies against four sites protect the animals from lethal infection with the virulent strain, Trinidad donkey. Out of the thirteen epitopes identified as being responsible for antiviral immunity, three are changes in the TC-83 strain, and six belong to two sites in the strain 230. The results obtained indicate the necessity for further improvement of the available vaccine preparations against this dangerous infectious disease.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12890148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
International symposium on 'Regulation and clinical importance of immunoglobulin E'. “免疫球蛋白E的调节和临床重要性”国际研讨会。
Biomedical science Pub Date : 1991-01-01
I S Gushchin
{"title":"International symposium on 'Regulation and clinical importance of immunoglobulin E'.","authors":"I S Gushchin","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12914594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intracellular factors determining the deposition of glycogen. The role of gluconeogenesis. 决定糖原沉积的细胞内因素。糖异生的作用。
Biomedical science Pub Date : 1991-01-01
V P Shelepov, V A Chekulaev, G R Pasha-Zade
{"title":"Intracellular factors determining the deposition of glycogen. The role of gluconeogenesis.","authors":"V P Shelepov,&nbsp;V A Chekulaev,&nbsp;G R Pasha-Zade","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The interrelationship between glycogen biosynthesis and do novo glucose formation in rats was studied by determining the oxaloacetic acid, ATP, and glycogen levels in the tissues of healthy starved rats and during the growth of transplantable hepatomas with different rates of growth. From the results obtained it was proposed that glycogen biosynthesis and de novo glucose formation are mutually enhanced and coupled processes. The validity of this conclusion was confirmed by in vitro model experiments involving the use of isolated-liver perfusion. Results of a multiple regression analysis indicated that the efficiency of gluconeogenesis and glycogen deposition is determined by the supply of oxygen to the liver, by the oxaloacetic acid content of this organ, and also by the degree of reduction of the glucose precursor. It was also shown that the ability of glucocorticoids and glucagon to enhance gluconeogenesis is dependent on the supply of oxygen to the liver. From the results of this in vivo and in vitro comparative study it is concluded that deposition of glycogen in the liver and possibly also in the brain is determined by the efficiency of gluconeogenesis.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12914618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of RNA replicase subunits responsible for initiation of RNA synthesis of tick-borne encephalitis virus by affinity labelling. 用亲和标记法鉴定蜱传脑炎病毒启动RNA合成的RNA复制酶亚基。
Biomedical science Pub Date : 1991-01-01
O V Morozova, N A Belyavskaya, E F Zaychikov, E A Kvetkova, A A Mustaev, A G Pletnev
{"title":"Identification of RNA replicase subunits responsible for initiation of RNA synthesis of tick-borne encephalitis virus by affinity labelling.","authors":"O V Morozova,&nbsp;N A Belyavskaya,&nbsp;E F Zaychikov,&nbsp;E A Kvetkova,&nbsp;A A Mustaev,&nbsp;A G Pletnev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Porcine embryo kidney cells infected by tick-borne encephalitis virus (TBEV) were fractionated into nuclear, membrane, and cytoplasmic fractions. To identify proteins involved in the initiation of RNA replication at different stages of infection a highly specific affinity labelling technique was used. In samples of the nuclear fraction taken from cells 45 h after infection (late stage), affinity labelling with aldehyde-containing derivatives of ATP and elongation of this label with [alpha-32P]GTP identified a polypeptide with a molecular mass of about 69 kDa. By means of affinity labelling with aldehyde-containing analogues of GMP, GDP, and GTP as initiation substrates and [alpha-32P]ATP as the elongation substrate, a polypeptide of 100 kDa was selectively modified in the nuclear fraction of cells at the early stages of infection (8 h). These proteins were immunostained with TBEV-specific antibodies, and were identified as the nonstructural TBEV proteins NS3 and NS5, respectively. It was concluded that NS3 and NS5 take part in the initiation of TBEV genome replication at the late and early stages of infection, respectively.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12935290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The UK-USSR medical exchange programme. 英苏医疗交流计划。
Biomedical science Pub Date : 1991-01-01
C Collier
{"title":"The UK-USSR medical exchange programme.","authors":"C Collier","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12959066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enzyme-linked immunoassay and electrophoretic separation of collagens secreted by cultured skin fibroblasts of patients with Ehlers-Danlos and Marfan syndromes. ehers - danlos和Marfan综合征患者皮肤成纤维细胞分泌胶原的酶联免疫分析和电泳分离
Biomedical science Pub Date : 1991-01-01
N V Borisova, E E Safronova, K D Krasnopol'skaya, O E Blinnikova, S A Polyudov
{"title":"Enzyme-linked immunoassay and electrophoretic separation of collagens secreted by cultured skin fibroblasts of patients with Ehlers-Danlos and Marfan syndromes.","authors":"N V Borisova,&nbsp;E E Safronova,&nbsp;K D Krasnopol'skaya,&nbsp;O E Blinnikova,&nbsp;S A Polyudov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method for the quantitation of the collagen types I, III, IV, and V, synthesized by cultured dermal fibroblasts is described. Collagens precipitated from the culture medium and intracellular collagens were detected by enzyme-linked immunosorbent assays with rabbit polyclonal monospecific antibodies. The assay could detect 10 ng protein per well, and intraassay coefficients of variation were less than 5%. Secreted collagens types I and III were also analysed by electrophoretic separation of [3H]-labelled alpha-chains under nonreducing and reducing conditions. These methods revealed a structural abnormality in one patient with clinical features of Marfan syndrome, and led to the postulation that the abnormality resulted from substitution of cysteine for another amino acid in collagen type I. In other patients an increase in the ratio of collagen type III:type I was detected, which may be secondary to an unknown biochemical defect.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13002154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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