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Amino acid sequence determination of vaccinia virus immunodominant protein p35 and identification of the gene. 牛痘病毒免疫优势蛋白p35的氨基酸序列测定及基因鉴定。
Biomedical science Pub Date : 1991-01-01
Chertov OYu, I N Telezhinskaya, E V Zaitseva, T B Golubeva, V V Zinov'ev, L G Ovechkina, L B Mazkova, E G Malygin
{"title":"Amino acid sequence determination of vaccinia virus immunodominant protein p35 and identification of the gene.","authors":"Chertov OYu,&nbsp;I N Telezhinskaya,&nbsp;E V Zaitseva,&nbsp;T B Golubeva,&nbsp;V V Zinov'ev,&nbsp;L G Ovechkina,&nbsp;L B Mazkova,&nbsp;E G Malygin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A major immunodominant envelope protein of vaccinia virus (protein p35) was purified by extraction from virions with the nonionic detergent Nonidet P-40. The protein was cleaved with cyanogen bromide. Four homogeneous peptides were isolated and their N-terminal amino acid sequences determined. A computer search of a protein-sequence data bank revealed complete identity of the determined sequences with sequences 44-63, 144-149, 154-165, and 224-238 of ORF H3 of the HindIII-H fragment of the vaccinia virus genome (Rosel et al 1986). It has therefore been established that the immunodominant protein p35 of vaccinia virus is encoded by the gene in the HindIII-H fragment of the vaccinia virus genome.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12935361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Estimation and comparison of lysosomal and cytoplasmic pH of human fibroblasts from healthy donors and patients with lysosomal storage diseases. 健康供体和溶酶体贮积症患者成纤维细胞溶酶体和细胞质pH值的测定和比较。
Biomedical science Pub Date : 1991-01-01
T S Ivleva, T A Ogloblina, L L Litinskaya, Wiederschain GYa
{"title":"Estimation and comparison of lysosomal and cytoplasmic pH of human fibroblasts from healthy donors and patients with lysosomal storage diseases.","authors":"T S Ivleva,&nbsp;T A Ogloblina,&nbsp;L L Litinskaya,&nbsp;Wiederschain GYa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Measurements were made of the pH of cytoplasm and lysosomes of cultured skin fibroblasts from healthy donors and from patients with lysosomal storage diseases (mannosidosis, Fabry's disease, and Krabbe's disease), and the effects of sucrose loading on normal fibroblasts were studied. The cytoplasmic pH of the pathological cells did not differ from control values, but the intralysosomal pH was significantly higher in sucrose-loaded normal fibroblasts and in cells from a patient with mannosidosis and from another with Fabry's disease. The change in pH observed accorded with an increase in size of the organelles, owing to accumulation of nonhydrolyzable compounds. In fibroblasts from a patient with Krabbe's disease, which do not store nonhydrolyzable compounds, there was no increase either in intralysosomal pH or in lysosome size. It is suggested that the decrease in the pH difference between the cytoplasm and lysosomes in the pathological cells leads to inhibition of catabolic processes in lysosomes and to some changes in the intracellular transport of these vesicles.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12959061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antitoxic properties of a preparation of glutathione S-transferase. 一种谷胱甘肽s -转移酶制剂的抗毒性。
Biomedical science Pub Date : 1991-01-01
E V Kalinina, V N Nechaev, A N Saprin
{"title":"Antitoxic properties of a preparation of glutathione S-transferase.","authors":"E V Kalinina,&nbsp;V N Nechaev,&nbsp;A N Saprin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pretreatment of rats with a preparation of the cationic form of rat liver cytosolic glutathione S-transferase 1-3 h before injection of a dose of N-nitroso-N-diethylamine equivalent to the LD50 of the drug completely protects the animals against the toxic effects of the drug. The mechanism of the protective action of glutathione S-transferase is discussed.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12959063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Management of a research group. 研究小组的管理。
Biomedical science Pub Date : 1991-01-01
T Chard
{"title":"Management of a research group.","authors":"T Chard","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It is generally believed that research and commerce are unique and different activities. However, the criteria of good business management can also be applied to research programmes, with much benefit to the latter. The objectives of both are fundamentally similar: in one case to produce goods and services, in the other to produce scientific papers. The most successful goods and services, and scientific projects, are those which are closely geared to the desires of consumers--whether the latter be the general public or large organisations. Science, like business, requires good leadership and good people-management. The characteristics of a successful science leader are often remarkably similar to those of a business leader. Individuals within any team must be carefully selected, adequately briefed, and then allowed to proceed with the job in hand. Obtaining resources for scientific research is also very similar to the funding of business activities--all come down to competition between different products and services. The end product of scientific research--the published paper--requires marketing in exactly the same way as a business product. Without such marketing the programme is almost always a failure. The effectiveness of a research programme must be monitored with the same care as would be devoted to a business. If research performance does not reach reasonably defined criteria, then resources are generally withdrawn--the equivalent of a bankruptcy in commerce.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12959121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Energy transduction in the myocardial contractile protein system under normal conditions and in toxi-allergic myocarditis. 正常情况下心肌收缩蛋白系统和毒素过敏性心肌炎的能量转导。
Biomedical science Pub Date : 1991-01-01
N V Karsanov, G V Sukoyan, D R Tatulashvili
{"title":"Energy transduction in the myocardial contractile protein system under normal conditions and in toxi-allergic myocarditis.","authors":"N V Karsanov,&nbsp;G V Sukoyan,&nbsp;D R Tatulashvili","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Energy transduction in purified myocardial myofibrils from normal rabbits and those having toxi-allergic myocarditis (TAM) was compared. In TAM a sharp decrease in the rate and value of ATP hydrolysis enthalpy released during free contraction of myofibrils takes place, and is accompanied by a rise in the amount of energy dissipated as heat. It is concluded that, at the early stages of shrinkage force generation during myofibril contraction in TAM, a breakdown of actin activation of Mg(2+)-ATPase activity for myosin occurs, the character of the bonds is changed, and the efficiency of myosin cross-bridge interaction with the protomeres of the actin thin filaments is decreased. The contractile process becomes wasteful.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12959123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structures of two myelopeptides affecting pain sensitivity. 影响疼痛敏感性的两种髓肽的结构。
Biomedical science Pub Date : 1991-01-01
L A Fonina, S A Gur'yanov, I V Nazimov, O G Yanovskii, L A Zakharova, A A Mikhailova, R V Petrov
{"title":"Structures of two myelopeptides affecting pain sensitivity.","authors":"L A Fonina,&nbsp;S A Gur'yanov,&nbsp;I V Nazimov,&nbsp;O G Yanovskii,&nbsp;L A Zakharova,&nbsp;A A Mikhailova,&nbsp;R V Petrov","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13001970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study of immunomodulatory properties of N-acetylmuramyl-L-alanyl-D-isoglutamine and N-acetylglucosaminyl-(beta 1----4)-N-acetylmuramyl-L-alanyl-D-isoglutamine. n -乙酰muramyl- l- alanyl- d -异谷氨酰胺和n -乙酰氨基葡萄糖-(β 1----4)- n -乙酰muramyl- l- alanyl- d -异谷氨酰胺免疫调节特性的研究。
Biomedical science Pub Date : 1991-01-01
I M Dozmorov, I I Kuzin, N I Lutsan, G V Lutsenko, A L Prokhorova, A M Sapozhnikov, T M Andronova, V T Ivanov
{"title":"Study of immunomodulatory properties of N-acetylmuramyl-L-alanyl-D-isoglutamine and N-acetylglucosaminyl-(beta 1----4)-N-acetylmuramyl-L-alanyl-D-isoglutamine.","authors":"I M Dozmorov,&nbsp;I I Kuzin,&nbsp;N I Lutsan,&nbsp;G V Lutsenko,&nbsp;A L Prokhorova,&nbsp;A M Sapozhnikov,&nbsp;T M Andronova,&nbsp;V T Ivanov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The immunomodulatory activities of two synthetic muramylpeptides (MP), a muramyl dipeptide and a glucosaminyl-muramyl dipeptide, have been compared and have been found to exhibit many common features in their effects. In addition, the differential effects of low and high concentrations of MP on the primary humoral immune response in vitro were examined in detail. At high concentrations MP augmented the frequency of induced T-suppressor cells, while at low concentrations the primary immune response was stimulated by enhancement of the antigen-presenting function of accessory cells and by increasing the frequency of induced T-helper cells.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13002803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PAF-like activity of O-acetylated sphingomyelin. 乙酰化鞘磷脂的paf样活性。
Biomedical science Pub Date : 1991-01-01
E V Berdyshev, O E Getmanova
{"title":"PAF-like activity of O-acetylated sphingomyelin.","authors":"E V Berdyshev,&nbsp;O E Getmanova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>O-acetylated sphingomyelin (Ac-SM) was found to cause aggregation of rabbit platelets in vitro. The Ac-SM-induced aggregation was accompanied by subsequent desensitisation of platelets to platelet-activating factor (PAF). The activity of Ac-SM exceeded that of acyl-PAF by about fourfold. BN 52021, a specific PAF-receptor antagonist, was found to inhibit the Ac-SM-induced aggregation. These results, together with earlier reports that sphingomyelin can inhibit the effects of PAF, suggest a new physiological function for sphingomyelin as a regulator of PAF-receptor binding. A search for enzymes to catalyse specifically the acetylation and deacetylation of sphingomyelin is required to confirm the physiological existence of sphingomyelin derivatives.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13003027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification and characterization of a thymocyte growth factor. 2. Biological activity of the thymocyte growth factor. 胸腺细胞生长因子的纯化及特性研究。2. 胸腺细胞生长因子的生物活性。
Biomedical science Pub Date : 1991-01-01
Talaev VYu, A A Yarilin, N I Sharova, V P Shichkin, T M Shuvaeva
{"title":"Purification and characterization of a thymocyte growth factor. 2. Biological activity of the thymocyte growth factor.","authors":"Talaev VYu,&nbsp;A A Yarilin,&nbsp;N I Sharova,&nbsp;V P Shichkin,&nbsp;T M Shuvaeva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A thymocyte growth factor, THGF, purified from the supernatant of a thymic T-lymphocyte-precursor cell line (TC.SC-1/2.0), stimulated the growth of splenocytes and thymocytes that had not been activated by a mitogen. Addition of suboptimal doses of mitogen or phorbol myristate acetate did not enhance the response. The growth factor could stimulate the growth of thymocytes synergistically with interleukin 2 but its action was not mediated by interleukin 2 or by interleukin 4. L3T4-Lyt2- thymocytes, Ig- splenocytes, Ig+ splenocytes, and alpha 1-thymosin-sensitive splenocytes were targets for THGF, which also stimulated the formation of haemopoietic spleen colonies in irradiated mice.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13003030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Treatment of experimental genital herpes with liposomal interferon. 脂质体干扰素治疗实验性生殖器疱疹。
Biomedical science Pub Date : 1991-01-01
G D Kobrinskii, V R Mel'nikov, V N Kulakov, N D L'vov, I M Bolotin, I F Barinskii
{"title":"Treatment of experimental genital herpes with liposomal interferon.","authors":"G D Kobrinskii,&nbsp;V R Mel'nikov,&nbsp;V N Kulakov,&nbsp;N D L'vov,&nbsp;I M Bolotin,&nbsp;I F Barinskii","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The therapeutic effect of genetically engineered interferon (reaferon) in the treatment of genital herpes in male guinea pigs was studied. The disease was induced in the animals by application of culture liquid containing herpes simplex virus type 2 (HSV-2) to the scarified skin of the penis. The affected sites were treated once daily, for 7 days, with different reaferon preparations 1-3 days after the symptoms of the disease had appeared. Reaferon preparations were most effective in the treatment of experimentally induced genital herpes when incorporated into liposomes, had a less pronounced therapeutic effect when mixed with 3% deoxymethylcellulose to form a hydrocolloid, and were least effective in aqueous solution. Incorporation of the antiviral agents acyclovir and Biolf-62 into liposomes also showed improved effectiveness, in comparison with the free compounds, in the treatment of genital herpes in guinea pigs.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13071369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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