由单核细胞和中性粒细胞产生自由基:血浆脂蛋白改变的可能原因。

Biomedical science Pub Date : 1991-01-01
O M Panasenko, T V Vol'nova, A N Osipov, O A Azizova, Vladimirov YuA
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引用次数: 0

摘要

用氧化人血浆脂蛋白(LP)检测新鲜分离的人血液单核细胞和中性粒细胞的活化,检测鲁米诺扩增的化学发光。活化伴随着超氧自由基的产生。这一发现通过测量超氧化物歧化酶对细胞色素c的敏感还原得到了证实。单核细胞或中性粒细胞与低密度脂蛋白(LDL)孵育导致脂质过氧化(LPO)产物的积累,通过2-硫代巴比妥酸试验进行了检测。抑制分析的数据表明,羟基自由基清除剂甘露醇在两种细胞类型的LDL孵育期间对LPO产物的积累没有明显的影响。然而,过氧化氢酶、超氧化物歧化酶、金属离子螯合剂去铁胺和EDTA,以及自由基清除剂丁基羟基甲苯,显著降低了培养基中LPO产物的积累——LDL与单核细胞孵养后分别降低了88%、67%、38%、52%和47%,LDL与中性粒细胞孵养后分别降低了65%、47%、41%、65%和100%。这些结果表明,氧化LP对单核细胞和中性粒细胞的激活增强了LPO, LPO通过超氧化物依赖的自由基机制进行,并由过渡金属催化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Free-radical generation by monocytes and neutrophils: a possible cause of plasma lipoprotein modification.

The activation of freshly isolated human blood monocytes and neutrophils monitored by oxidized human plasma lipoproteins (LP) was measured by detecting luminol-amplified chemiluminescence. The activation was accompanied by production of superoxide radicals. This finding was confirmed by measuring superoxide dismutase-sensitive reduction of cytochrome c. Incubation of monocytes or neutrophils with low-density lipoproteins (LDL) resulted in the accumulation of lipid peroxidation (LPO) products which were assayed by the 2-thiobarbituric acid test. Data from inhibitory analysis suggest that the hydroxyl radical scavenger, mannitol, had no appreciable effect on the accumulation of LPO products during the incubation of LDL with either cell type. However, catalase, superoxide dismutase, the metal ion chelators desferrioxamine and EDTA, as well as the free radical scavenger, butylated hydroxytoluene, markedly decreased the accumulation of LPO products in the medium--by 88%, 67%, 38%, 52%, and 47%, respectively, after incubation of LDL with monocytes, and by 65%, 47%, 41%, 65%, and 100% after incubation of LDL with neutrophils. These results indicate that activation of monocytes and neutrophils by oxidized LP intensifies LPO which proceeds via a free-radical mechanism that is superoxide-dependent and is catalyzed by transition metals.

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