Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress最新文献_第6页

[Transfection of follicular thyroid gland carcinoma cells with human TSH receptor changes growth, invasion and adhesion]. [转染人TSH受体的滤泡性甲状腺癌细胞改变其生长、侵袭和粘附]。

Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress Pub Date : 1998-01-01

T Hoelting, Q Y Duh, O H Clark, C Herfarth

{"title":"[Transfection of follicular thyroid gland carcinoma cells with human TSH receptor changes growth, invasion and adhesion].","authors":"T Hoelting, Q Y Duh, O H Clark, C Herfarth","doi":"","DOIUrl":"","url":null,"abstract":"TSH is the classic stimulator of thyroid cell function. Clinically, treatment with thyroxin to suppress TSH decreased the risk of thyroid cancer recurrence and improved patient survival. This study analyzed the effect of stably transfected human TSH receptor cDNA in an established model of metastatic follicular thyroid cancer cells (FC) compared to wild type FTC. Wild type FTC lack TSH receptors and do not depend on TSH for growth. However, they contain thyroglobulin, have intact thyroid functions and response to TSH. We tested growth, invasion, and adhesion of transfected tumor cells (FTC-TSHr) compared to parental cells. All transfected FTC-TSHr expressed TSHr mRNA. Compared to wild type cells invasion and growth of TSHr-transfected FTC were significantly inhibited (p < 0.001). All FTC adhered best to collagen IV and fibronectin. Compared to parental cells adhesion of unstimulated FTC-TSHr was significantly enhanced (p < 0.001). These in vitro data underline the important role of the human TSH receptor as the main regulator of thyroid growth and functions.","PeriodicalId":77239,"journal":{"name":"Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress","volume":"115 Suppl I","pages":"281-4"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40831342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Expression of polysialylated NCAM on neuroblastomas of various histology and clinical stages]. [多唾液化NCAM在不同组织学和临床分期神经母细胞瘤中的表达]。

Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress Pub Date : 1998-01-01

S Glüer, M Zense, E Radtke, D von Schweinitz

引用次数: 0

[Molecular diagnosis and clinical consequences in families with HNPCC syndrome]. [HNPCC综合征家庭的分子诊断和临床后果]。

Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress Pub Date : 1998-01-01

S Pistorius, J Plaschke, C Kruppa, J Rüschoff, M Nagel, H D Saeger, H K Schackert

{"title":"[Molecular diagnosis and clinical consequences in families with HNPCC syndrome].","authors":"S Pistorius, J Plaschke, C Kruppa, J Rüschoff, M Nagel, H D Saeger, H K Schackert","doi":"","DOIUrl":"","url":null,"abstract":"Thirteen families were included in our HNPCC surveillance program, eight of which met strict and three incomplete Amsterdam criteria. Two index patients were younger than 35 years of age. Tumors of all index persons showed microsatellite instabilities in at least 50% of the ten markers studied. Immunohistochemical analysis of tumor sections was performed using antibodies against hMLH1 and hMSH2 proteins in order to identify the mutated gene, which is not expressed in the tumor. Coding regions of hMLH1 and hMSH2 genes were amplified by PCR from genomic DNA and sequenced. In nine families pathogenetic mutations all resulting in a truncated protein, could be identified. Furthermore, 21 intron and exon polymorphisms were found. Since July 1997 we have offered surgical and genetic counseling to families with hereditary cancer syndromes in a special outpatient clinic. In addition to 13 index patients three asymptomatic gene carriers were included and eight noncarriers were excluded from the HNPCC surveillance program, as recommended by the HNPCC study group of Germany. Molecular diagnosis has considerable clinical implication in hereditary nonpolyposis colorectal cancer (HNPCC) families with respect to family members who actually have the disease as well as gene carriers and noncarriers.","PeriodicalId":77239,"journal":{"name":"Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress","volume":"115 Suppl I","pages":"293-7"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40831345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Different integrin-induced adhesion of highly liver metastatic and little metastatic colon carcinoma cells in an extracellular matrix]. [不同整合素诱导的高肝转移和低转移结肠癌细胞在细胞外基质中的粘附]。

Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress Pub Date : 1998-01-01

J Haier, M Nasralla, H J Buhr, G L Nicolson

{"title":"[Different integrin-induced adhesion of highly liver metastatic and little metastatic colon carcinoma cells in an extracellular matrix].","authors":"J Haier, M Nasralla, H J Buhr, G L Nicolson","doi":"","DOIUrl":"","url":null,"abstract":"Poorly and highly liver metastatic colon carcinoma cell lines have different integrin-mediated adhesion to extracellular matrix. Specific integrin-mediated interactions between tumor cells and extracellular matrix (ECM) in the host organs are important for organ-specific metastasis. In colon carcinoma integrin expression differs depending on the metastatic potential of the tumor. Integrin-mediated adhesion of poorly (HT-29P) and highly liver-metastatic (HT-29LMM) colon carcinoma to extracellular matrix (ECM; Collagen I-C I, Collagen IV-C IV, Laminin LN, Fibronectin FN, Vitronectin VN) was investigated. HT-29LMM showed significant better adhesion to LN (45% vs. 26%; p < 0.001) and FN 20% vs. 1%; p < 0.001). No adhesion was found to VN. RGD-oligopeptides completely inhibited adhesion to FN. Using inhibition with anti-integrin-mAB it was shown, that adhesion to C I and C IV is mediated by alpha 2 beta 1-integrin, adhesion to LN by alpha 6 beta 1 and adhesion to FN by alpha v beta 1. These results have shown that adhesion of HT-29 cells is mediated by different integrins depending on ECM components. Poorly and highly metastatic cells possessed different patterns of adhesion to various substrates.","PeriodicalId":77239,"journal":{"name":"Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress","volume":"115 Suppl I","pages":"307-13"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40831348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Tissue protection by ischemic preconditioning depends mainly on expression of heat shock protein 32]. [缺血预处理对组织的保护主要依赖于热休克蛋白的表达32]。

Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress Pub Date : 1998-01-01

F Rösken, D Kubulus, M Amon, M Rücker, I Bauer, M D Menger

引用次数: 0

[Measuring blood flow velocity in healthy and indomethacin-induced inflammation in rat small intestine serosa with FITC-marked erythrocytes]. [用fitc标记红细胞测量健康和消炎痛诱导的大鼠小肠浆膜血流速度]。

Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress Pub Date : 1998-01-01

C F Krieglstein, C Anthoni, M G Laukötter, H U Spiegel, K W Schmid, G Schürmann

引用次数: 0

[Protective effect of glutamine on microcirculation of the intestine in experimental colitis]. 谷氨酰胺对实验性结肠炎患者肠道微循环的保护作用。

Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress Pub Date : 1998-01-01

M Kruschewski, S Perez-Cantó, A Hübotter, T Foitzik, H J Buhr

引用次数: 0

[Growth factor a/b-FGF and PDGF have no effect on development of inflammatory tumor in chronic pancreatitis]. [生长因子a/b-FGF和PDGF对慢性胰腺炎炎性肿瘤的发展无影响]。

Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress Pub Date : 1998-01-01

I Scheurlen, J R Izbicki, H Sauer, M Stabenow, C Bloechle, W T Knoefer, C E Broelsch

{"title":"[Growth factor a/b-FGF and PDGF have no effect on development of inflammatory tumor in chronic pancreatitis].","authors":"I Scheurlen, J R Izbicki, H Sauer, M Stabenow, C Bloechle, W T Knoefer, C E Broelsch","doi":"","DOIUrl":"","url":null,"abstract":"Extensive fibrosis combined with the development of pseudocysts, calcifications and concomittant loss of acinar cells are the characteristic features in the pathogenesis of chronic pancreatitis. In some patients an inflammatory mass is generated which is most frequently located in the pancreatic head. The inflammatory pancreatic head tumor has been postulated to be the pace maker of the course of the disease. Over expression of growth factors (GF) has been discussed to be the molecular basis of focal development of fibrosis. Aim of this study was to correlate the expression of GF-m-RNA (acidic und basic fibroblast growth factor: a/b FGF; plateled derived growth factor: PDGF-A/B) in pancreatic specimens from patients with chronic pancreatitis taken from different parts of the pancreatic gland to the generation of an inflammatory mass in the pancreatic head. Using quantitative PCR, northern blotting and in-situ-hybridisation m-RNA expression of a/b FGF and PDGF-A/B was nearly identical in the pancreatic head, corpus and tail. Furthermore the expression of these GF did not correlate to the development of an inflammatory mass in the pancreatic head. From the results of this study a causal relation between expression of these GF and the development of an inflammatory mass in the pancreatic head could not be confirmed.","PeriodicalId":77239,"journal":{"name":"Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress","volume":"115 Suppl I","pages":"541-5"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40831491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Experimental evaluation of dynamic MRI for quantifying liver perfusion]. 动态MRI定量肝脏灌注的实验评价。

Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress Pub Date : 1998-01-01

C Zapletal, A Mehrabi, J Scharf, T Hess, T Kraus, C Herfarth, E Klar

引用次数: 0

[A new method of orthotopic liver transplantation from the guinea pig to the rat]. 一种豚鼠原位肝移植到大鼠的新方法。

Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress Pub Date : 1998-01-01

W Mark, P Hechenleitner, D Candinas, P Hengster, G Klima, J Feichtinger, R Margreiter

引用次数: 0