Alcohol最新文献

{"title":"Age-related impact of phenobarbital in suppressing prenatal alcohol exposure-related seizures in developing rats","authors":"Tengfei Li ,&nbsp;George Luta ,&nbsp;Prosper N'Gouemo","doi":"10.1016/j.alcohol.2024.12.007","DOIUrl":"10.1016/j.alcohol.2024.12.007","url":null,"abstract":"<div><div>Prenatal alcohol exposure (PAE) during pregnancy can increase the prevalence of <em>N</em>-methyl-<span>d</span>-aspartate (NMDA)-induced generalized tonic-clonic seizures (GTCSs) in developing rats. However, it is unclear whether phenobarbital (PB) can suppress these PAE-related seizures. To explore this knowledge gap, we investigated the effects of acute PB treatment on NMDA-induced seizures in postpartum rats, prenatally exposed to alcohol on gestational day 18 (GD18), at two developmental stages: day 7 (P7), the equivalent of pre-term neonates, and day 15 (P15), the equivalent of full-term neonates. Timed-pregnant female Sprague–Dawley rats were given a single dose of alcohol or its vehicle on GD18 during the second-trimester equivalent. Male and female postpartum rats were tested for the effectiveness of single-dose treatment with either PB or its vehicle in suppressing NMDA-induced seizures. These seizures include wild running-like behavior (WRLB), flexion seizures (FSs), clonic seizures (CSs), generalized tonic-clonic seizures (GTCSs), and tonic seizures (TSs) in P7 and P15 rats. Analyses revealed that P7 rats were more likely to develop GTCSs after PB administration than P15 rats; this effect was associated with shorter latencies to develop NMDA-induced seizures. Moreover, PAE-related seizure severity is less responsive to PB treatment in P7 rats than in P15 rats. These findings suggest that the PAE-related GTCS model in P7 rats can be used to investigate the mechanisms underlying PB-resistant seizures in developing rats.</div></div>","PeriodicalId":7712,"journal":{"name":"Alcohol","volume":"123 ","pages":"Pages 39-50"},"PeriodicalIF":2.5,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the relationship between mental health and AUDIT score among older sexual and gender minorities","authors":"Nathaniel Albright ,&nbsp;Ethan Morgan","doi":"10.1016/j.alcohol.2024.12.005","DOIUrl":"10.1016/j.alcohol.2024.12.005","url":null,"abstract":"<div><h3>Introduction</h3><div>Alcohol use, and its relationship with mental health outcomes, remains a public health priority. Yet, little research has focused on this association among aging sexual and gender minority (SGM) populations with even less dedicated to the unique issues of those aging with HIV, a gap we begin to fill here.</div></div><div><h3>Methods</h3><div>Data for this analysis originated from the Columbus Healthy Aging Project (CHAP), a cross-sectional survey among adults ≥50 years who reside in the Columbus, Ohio. Multivariable linear regression models were utilized to assess the relationship between alcohol use (via AUDIT score) and several mental health outcomes (e.g., depression, anxiety, perceived stress, and sexual orientation microaggressions), adjusting for demographic characteristics and other risk factors. Models were assessed for moderation by self-reported HIV status.</div></div><div><h3>Results</h3><div>Among the entire sample (N = 787), mean perceived stress score was 18.2 (SD = 5.5), mean anxiety score was 9.1 (5.9), and mean depression score was 9.9 (SD = 6.7). 32 (7.4%) self-reported as PLWH. Among those reporting any alcohol use, mean AUDIT score use was 10.5 (SD = 10.9). Each of the mental health outcome measures were positively associated with AUDIT score. Meanwhile, there was significant moderation of each of the mental health outcome measures by HIV status, suggesting a stronger association with AUDIT score in each case.</div></div><div><h3>Conclusion</h3><div>Our results suggest that there are broad stressors impacting alcohol use not only among older SGM broadly but in particular among PLWH. Although a diverse set of results, these data highlight the need for more research on alcohol use among aging SGM populations, particularly PLWH and those identifying as a different gender identity.</div></div>","PeriodicalId":7712,"journal":{"name":"Alcohol","volume":"123 ","pages":"Pages 51-56"},"PeriodicalIF":2.5,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analgesic effect of oxytocin in alcohol-dependent male and female rats","authors":"John Marendes (Jr.),&nbsp;Marissa A. Muench,&nbsp;Camille L. Young,&nbsp;Amira A. Ghaly,&nbsp;Brendan J. Tunstall","doi":"10.1016/j.alcohol.2024.12.002","DOIUrl":"10.1016/j.alcohol.2024.12.002","url":null,"abstract":"<div><h3>Introduction</h3><div>Chronic alcohol exposure in humans and rodents causes tolerance to the analgesic effects of alcohol, and enhances pain sensitivity during alcohol withdrawal (i.e., hyperalgesia). The available literature suggests a bidirectional enhancement between chronic alcohol consumption and chronic pain sensitivity. We previously found that oxytocin administration could reduce alcohol consumption in alcohol-dependent rats, and now hypothesize that oxytocin, through analgesic action in the central nervous system, could ameliorate the hyperalgesia induced by alcohol-dependence. To test this hypothesis, we assessed the ability of central and peripheral oxytocin administration to alter thermal (Hargreaves assay) and mechanical (Von Frey assay) pain sensitivity, in male and female rats, made alcohol dependent through repeated cycles of chronic-intermittent ethanol-vapor exposure (CIEV; compared to air-exposed controls).</div></div><div><h3>Methods</h3><div>Male and female cohorts of Wistar rats were surgically prepared with an ICV cannula and assigned to two groups matched in terms of initial response in the Hargreaves assay. Rats in the alcohol dependent group were exposed to chronic-intermittent alcohol-vapor, while air-exposed control rats were exposed only to room air and served as the control group. The thermal nociception sensitivity of all rats was monitored via weekly Hargreaves assay to determine alcohol-dependence-induced hyperalgesia in dependent rats. Next, rats were ICV administered oxytocin (0, 0.5, or 5 μg in 2.5 μL saline) prior to Hargreaves testing (Experiment 1) or Von Frey testing (Experiment 2). Finally, rats were IP administered oxytocin (0, 0.1, or 1 mg/kg) prior to Hargreaves testing (Experiment 3) or Von Frey testing (Experiment 4). In a follow-up experiment, female rats were tested to directly compare three methods for applying the Von Frey test.</div></div><div><h3>Results</h3><div>Male and female alcohol-dependent rats developed hyperalgesia, observed in the Hargreaves assay (Experiment 1 &amp; 3), however, hyperalgesia was not so readily observed when the same rats were tested in the Von Frey assay (Experiments 2 &amp; 4, with the exception of female rats in Experiment 4; follow-up testing indicated that the method of Von Frey test employed is likely important to explain this discrepancy). In both the Hargreaves and Von Frey assays, and in both male and female rats, following central or peripheral administration, oxytocin produced analgesia similarly in both alcohol dependent rats and air-exposed controls.</div></div><div><h3>Conclusion</h3><div>Together, these data suggest the oxytocin system could be targeted to produce therapeutic action in disease that produce hyperalgesia such as in alcohol dependence. We discuss methodological considerations and future experiments that could further elucidate a role for oxytocin in the overlapping neurobiology of alcohol dependence and chronic pain.</div></div>","PeriodicalId":7712,"journal":{"name":"Alcohol","volume":"123 ","pages":"Pages 27-38"},"PeriodicalIF":2.5,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced fear extinction through infralimbic perineuronal net digestion: The modulatory role of adolescent alcohol exposure","authors":"J. Daniel Obray ,&nbsp;Adam R. Denton ,&nbsp;Jayda Carroll-Deaton ,&nbsp;Kristin Marquardt ,&nbsp;L. Judson Chandler ,&nbsp;Michael D. Scofield","doi":"10.1016/j.alcohol.2024.12.006","DOIUrl":"10.1016/j.alcohol.2024.12.006","url":null,"abstract":"<div><div>Perineuronal nets (PNNs) are specialized components of the extracellular matrix that play a critical role in learning and memory. In a Pavlovian fear conditioning paradigm, degradation of PNNs affects the formation and storage of fear memories. This study examined the impact of adolescent intermittent ethanol (AIE) exposure by vapor inhalation on the expression of PNNs in the adult rat prelimbic (PrL) and infralimbic (IfL) subregions of the medial prefrontal cortex. Results indicated that following AIE, the total number of PNN positive cells in the PrL cortex increased in layer II/III but did not change in layer V. Conversely, in the IfL cortex, the number of PNN positive cells decreased in layer V, with no change in layer II/III. In addition, the intensity of PNN staining was significantly altered by AIE exposure, which narrowed the distribution of signal intensity, reducing the number of high and low intensity PNNs. Given these changes in PNNs, the next experiment assessed the effects of AIE and PNN digestion on extinction of a conditioned fear memory. In Air control rats, digestion of PNNs by bilateral infusion of Chondroitinase ABC (ChABC) into the IfL cortex enhanced fear extinction and reduced contextual fear renewal. In contrast, both fear extinction learning and contextual fear renewal remained unchanged following PNN digestion in AIE exposed rats. These results highlight the sensitivity of prefrontal PNNs to adolescent alcohol exposure and suggest that ChABC-induced plasticity is reduced in the IfL cortex following AIE exposure.</div></div>","PeriodicalId":7712,"journal":{"name":"Alcohol","volume":"123 ","pages":"Pages 57-67"},"PeriodicalIF":2.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"7. Alcohol consumption affects MSC differentiation capacity in orthopaedic trauma patients","authors":"A.B. Boyd,&nbsp;E.L. Murdoch,&nbsp;J.J. Callaci","doi":"10.1016/j.alcohol.2024.10.010","DOIUrl":"10.1016/j.alcohol.2024.10.010","url":null,"abstract":"","PeriodicalId":7712,"journal":{"name":"Alcohol","volume":"121 ","pages":"Pages 209-210"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"32. Alcohol-induced tissue injury at the gut-liver-brain axis is more severe in diabetic mice","authors":"N. Shashikanth ,&nbsp;L. Basa ,&nbsp;R. Rajenthiran,&nbsp;C. Nguyen,&nbsp;P. Raju,&nbsp;S.C. Lee,&nbsp;C. Shekhar,&nbsp;F. Giorgianni,&nbsp;R.K. Rao","doi":"10.1016/j.alcohol.2024.10.035","DOIUrl":"10.1016/j.alcohol.2024.10.035","url":null,"abstract":"","PeriodicalId":7712,"journal":{"name":"Alcohol","volume":"121 ","pages":"Page 217"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143127755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"12. Role of Foxp3+ regulatory T cells in chronic ethanol consumption and sepsis pathogenesis","authors":"M.B. Gutierrez,&nbsp;C.M. Coopersmith,&nbsp;M.L. Ford","doi":"10.1016/j.alcohol.2024.10.015","DOIUrl":"10.1016/j.alcohol.2024.10.015","url":null,"abstract":"","PeriodicalId":7712,"journal":{"name":"Alcohol","volume":"121 ","pages":"Page 211"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143127862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"20. Myeloid TLR4 deficient mice are not protected from alcohol-mediated liver injury and inflammation: contribution of hepatocytes and neutrophils","authors":"A. Mandal,&nbsp;A. Ratna,&nbsp;J. Thanikasalam,&nbsp;E. Kurt-Jones,&nbsp;P. Mandrekar","doi":"10.1016/j.alcohol.2024.10.023","DOIUrl":"10.1016/j.alcohol.2024.10.023","url":null,"abstract":"","PeriodicalId":7712,"journal":{"name":"Alcohol","volume":"121 ","pages":"Pages 213-214"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2. Ethanol exposure impairs LC3-associated phagocytosis via IL-1β-ATG16L1 pathway in macrophages","authors":"A. Ahuja,&nbsp;B. Pant,&nbsp;E. Cross,&nbsp;D. Shrestha,&nbsp;S. Abraham,&nbsp;R. Scheraga,&nbsp;V. Vachharajani","doi":"10.1016/j.alcohol.2024.10.005","DOIUrl":"10.1016/j.alcohol.2024.10.005","url":null,"abstract":"","PeriodicalId":7712,"journal":{"name":"Alcohol","volume":"121 ","pages":"Page 208"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"36. mRNA profile of mouse large intestine epithelial cells after DSS colitis and ethanol exposure","authors":"M.M. Tschann ,&nbsp;A.R. Cannon ,&nbsp;M.N. Xi ,&nbsp;M.A. Choudhry","doi":"10.1016/j.alcohol.2024.10.039","DOIUrl":"10.1016/j.alcohol.2024.10.039","url":null,"abstract":"","PeriodicalId":7712,"journal":{"name":"Alcohol","volume":"121 ","pages":"Page 218"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}