American Journal of Reproductive Immunology最新文献

{"title":"Calycosin Protects Against Chronic Prostatitis via Regulating Cellular Pyroptosis","authors":"Heng Wang,&nbsp;Zhaofei Liu,&nbsp;Xiangjun Xu,&nbsp;Haitao Zhang,&nbsp;Lei He,&nbsp;Ming Li","doi":"10.1111/aji.70084","DOIUrl":"https://doi.org/10.1111/aji.70084","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Chronic prostatitis (CP), a frequent male urological disease, is featured with chronic pelvic pain and various discomfort. Calycosin has anti-inflammatory effect and its exact mechanism in CP remains unknown.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Our research was designed to explain the underlying mechanism of calycosin in CP and illustrate our findings in the rats and LPS-ATP-induced RWPE-1 cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The pathology of prostate tissues was analyzed by HE staining and histological inflammation score. The concentration of inflammatory factors were detected via ELISA assay. Besides, the representative biomarkers of oxidative stress were measured through detection kits. The ROS production, NLRP3 and GSDMD expression were evaluated by immunofluorescence or immumohistochemical staining. Furthermore, the expression of NF-kBp65 pathway-associated proteins and pyroptosis-associated proteins were detected by western blotting. RWPE-1 cells growth and pyroptosis were assessed by CCK-8 and flow cytometry, respectively. mRNA levels of pyroptosis markers were detected by qRT-PCR analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The CP model in vivo and in vitro was successfully established by carrageenan and LPS-ATP, respectively. Calycosin substantially ameliorated the pathological damage of prostate tissue. Moreover, calycosin treatment promoted RWPE-1 cells viability and reduced pyroptosis. Calycosin remarkably reduced the inflammatory factors secretion and oxidative stress markers. Calycosin stimulation effectively suppressed the pyroptosis, which was linked with inactivation of the NF-kBp65 signaling pathway. These beneficial effects of calycosin were similar to the NLRP3 inhibitor (MCC950).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>To sum up, our observations indicated that calycosin protects against CP via suppressing inflammation, oxidative stress, and pyroptosis through NF-kBp65 pathway, thereby serving as a promising therapy for CP treatment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"93 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Maternal and Fetal Plasma X Box Binding Protein 1 Levels Between Pregnancies With Fetal Growth Retardation and Healthy Controls","authors":"Sena Ünlü,&nbsp;Nizamettin Bozbay,&nbsp;Fikret Akyürek,&nbsp;Gökcen Örgül,&nbsp;Çetin Çelik","doi":"10.1111/aji.70094","DOIUrl":"https://doi.org/10.1111/aji.70094","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study aimed to compare maternal and cord serum X box binding protein 1 (XBP-1) levels in pregnant women with fetal growth restriction (FGR) with healthy pregnancies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>This prospective case-control study was conducted between January 1, 2022, and May 31, 2022, at the Gynecology and Obstetrics Clinic of Selçuk University Faculty of Medicine Hospital. Forty-three pregnant women and fetuses with isolated FGR with abdominal circumference (AC) or estimated fetal weight (EFW) below the 10th percentile according to ultrasonographic measurements constituted the study group; 43 healthy pregnant women and fetuses constituted the control group. Serum XBP-1 levels in prenatal maternal and cord blood were measured by ELISA and compared between the two groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The mean maternal blood XBP-1 level was 1910.22 ± 607.07 ng/L in the FGR group and 1638.89 ± 385.80 ng/L in the control group (<i>p</i> = 0.044). Cord blood XBP-1 levels were 1837.72 ± 942.67 and 1346.14 ± 664.09 ng/L in the study and control groups, respectively (<i>p</i> = 0.006). Maternal XBP-1 levels were found to discriminate FGR with a sensitivity of 80% and specificity of 60% at a value of 1405 ng/L. For cord blood XBP-1, 869.2 ng/L was the best cut-off, with 98% sensitivity and 67% specificity. In ROC analysis, the area under the curve was found to be 0.626 and 0.671, and <i>p</i> values were found to be 0.044 and 0.006 for maternal serum XBP-1 and cord blood XBP-1, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>In the study, serum XBP-1 levels in both maternal and fetal umbilical cord blood were found to be higher in patients with FGR. Considering the role of XBP-1 in metabolic pathways and cellular functions, XBP-1 may have an important role in the pathophysiology of FGR.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"93 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decreased IL-33 Expression in the Cervix in LPS-Induced Preterm Birth and the Potential Role of Mast Cells: A Murine Model","authors":"Sema Avci,&nbsp;Ciler Celik-Ozenci,&nbsp;Nilay Kuscu,&nbsp;Nayce Ilayda Bektas","doi":"10.1111/aji.70085","DOIUrl":"https://doi.org/10.1111/aji.70085","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Problem</h3>\u0000 \u0000 <p>In order to gain a deeper understanding of the mechanisms underlying LPS-mediated preterm birth, it is crucial to investigate the relationship between preterm birth and mast cells (MCs). Moreover, the role of antihistamines in inflammatory processes during pregnancy remains incompletely understood.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method of Study</h3>\u0000 \u0000 <p>CD-1 female mice were administered intrauterine lipopolysaccharide (LPS) via midline laparotomy to establish an inflammation-induced preterm birth model. The experimental groups (<i>n</i> = 6 per group) were formed as Nonpregnant and pregnant control, Sham, PBS, LPS, Cetirizine (CET) control, and two CET treatment groups (CET 10 mg/kg-low dose, and CET 20 mg/kg-high dose with LPS administration). Tissue samples were analyzed using immunohistochemistry and Western blot techniques.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Our findings suggest that MCs play a significant role in preterm birth, with LPS administration inducing MC dysfunction in the reproductive tract during pregnancy. Additionally, high doses of CET may support inflammatory responses. A particularly notable result was the reduction in interleukin-33 (IL-33) expression in the cervix during LPS-induced preterm birth. This suggests that IL-33 may serve as a potential biomarker for preterm birth in the cervix.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The effects of CET during LPS-mediated preterm birth appear to be dose-dependent, warranting further exploration of their role in this context.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"93 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143914034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Initial and Longitudinal Trajectories in Hemoglobin Levels Predict the Prognosis of Patients With Premature Rupture of Membranes","authors":"Ying-ying Chen,&nbsp;Hai-xing Huang,&nbsp;Min Xiao","doi":"10.1111/aji.70086","DOIUrl":"https://doi.org/10.1111/aji.70086","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Problem</h3>\u0000 \u0000 <p>Premature rupture of membranes (PROM) can lead to adverse maternal and neonatal outcomes. Identifying predictors of length of hospital stay (LOS) in PROM patients can aid in improving management and prognosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method of Study</h3>\u0000 \u0000 <p>The Medical Information Mart for Intensive Care IV database was searched to acquire data on PROM individuals. A univariable analysis, LASSO regression, and multivariable generalized linear model (GLM) were adopted to identify factors closely related to LOS. Next, the change trends in the key variable over multiple time points were determined using a latent class trajectory model (LCTM). The association of these trajectories with LOS was analyzed using GLMs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The retrospective study finally enrolled 868 samples. The significant factors included initial hemoglobin, chorioamnionitis, premature birth, and rupture to delivery interval &lt;24 h. Notably, higher initial hemoglobin level was independently associated with shorter LOS (<i>β</i> = −0.599; 95% CI = [−0.846, −0.352]; <i>p</i> &lt; 0.05). The stratified analysis confirmed this association in all subgroups except those with chorioamnionitis. The LCTM identified three hemoglobin trajectories. Compared to other trajectory groups, the “lowest, declining” group had longer LOS (<i>p</i> &lt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>PROM patients with lower initial hemoglobin levels and the “lowest, declining” trajectory group were linked to longer LOS. Monitoring and managing hemoglobin levels is important in improving patient outcomes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"93 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aji.70086","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143908990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moxibustion Enhances Ovarian Function by Inhibiting the Th17/IL-17 Pathway and Regulating Gut Microbiota in POI Rats","authors":"Zheng Luo,&nbsp;Xinru Lu,&nbsp;Tianyi Zhang,&nbsp;Shijie Shi,&nbsp;Rui Zhao,&nbsp;Yizhi He,&nbsp;Hanyue Yao,&nbsp;Weina Zhu,&nbsp;Cairong Zhang","doi":"10.1111/aji.70082","DOIUrl":"https://doi.org/10.1111/aji.70082","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Problem</h3>\u0000 \u0000 <p>Premature ovarian insufficiency (POI) is a significant cause of female infertility, severely impacting physical and mental health. Current treatments, primarily hormone replacement therapy, fail to restore ovarian function and may cause adverse effects. Moxibustion, a traditional Chinese medicine therapy, has shown potential in treating POI, but its mechanisms remain unclear. This study investigated the therapeutic effects of moxibustion on POI rats and explored its underlying mechanisms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method of Study</h3>\u0000 \u0000 <p>A POI rat model was established using cyclophosphamide, and moxibustion was applied daily to the CV4 and SP6 acupoints for 4 weeks. We analyzed hormone levels, estrous cycles, follicle count, and gut microbiota. Transcriptomic and metagenomic sequencing were performed to identify potential pathways. Network pharmacology was used to predict active components and targets.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Moxibustion restored estrous cycles, improved hormonal imbalances, and increased ovarian reserve function. Network pharmacology identified five active components in moxa, and based on the results of network pharmacology and transcriptome sequencing, we believe that the regulation of the IL-17 pathway is the key mechanism. Further experiments showed moxibustion downregulated the Th17/IL-17 pathway, reduced key proteins such as IL-17R, NF-κB, MMP3, IκBα, IL-1β, MMP9, TRAF6, and Cox2. Flow cytometry confirmed a decrease in Th17 cell proportion. Gut microbiota analysis revealed that moxibustion enhanced microbial diversity and modulated specific bacterial species, which correlated with improved hormone levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Moxibustion has a therapeutic effect on POI rats by regulating the Th17/IL17 pathway and gut microbiota, which provides evidence for the clinical application of moxibustion.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"93 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on MTHFR (C677T & A1298C) Gene Polymorphisms in the Condition of Glucose Intolerance During Pregnancy","authors":"Bunga Papa Kusuma,&nbsp;D. Lakshmi Lalitha,&nbsp;Nagarjuna Sivaraj,&nbsp;Vijaya Sirisha Balaga,&nbsp;Arun Kumar Rao Panchanani,&nbsp;L V Simhachalam Kutikuppala,&nbsp;S. Sai Roshan,&nbsp;K. Kavya,&nbsp;Golla Varshitha","doi":"10.1111/aji.70081","DOIUrl":"https://doi.org/10.1111/aji.70081","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Gestational diabetes mellitus (GDM) is a prevalent medical complication in pregnancy, characterized by glucose intolerance. The global expected prevalence of GDM is approximately 15.1%. This study builds upon previous research by investigating elevated hematological parameters and exploring MTHFR gene polymorphisms in GDM.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>This study included 304 pregnant women, comprising 152 patients with GDM and an equal number of normal pregnant women. Employing PCR-RFLP techniques, we identified MTHFR gene polymorphisms (C677T &amp; A1298C) associated with gestational diabetes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant associations were found in gestational age and platelet count, indicating their relevance to GDM risk. The odds ratios for both MTHFR A1298C (<i>p</i> value: 0.034; OR = 0.7; 95% CI: 0.5021–0.9758) and MTHFR C677T (<i>p</i> value: 0.008; OR = 0.5453; 95% CI: 0.3292–0.9011) gene polymorphisms demonstrated an increased risk of GDM development.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The study reveals that elevated platelet count and polymorphs increase the risk of developing GDM in pregnant women. Gestational age also plays a role. The study also finds a link between the MTHFR C677T SNP and the MTHFR A1298C gene polymorphism and the risk of gestational diabetes in Andhra Pradesh. This gives us new information about genetic and hematological factors.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"93 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Evidence of Maternal Infection With Chlamydia trachomatis and Preeclampsia Risk","authors":"Brandie DePaoli Taylor,&nbsp;Catherine L. Haggerty,&nbsp;Emmanuel Amabebe,&nbsp;Lauren S. Richardson","doi":"10.1111/aji.70080","DOIUrl":"https://doi.org/10.1111/aji.70080","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Chlamydia trachomatis</i> is the most common bacterial sexually transmitted infection (STI) in the United States. Ascending <i>C. trachomatis</i> can cause pelvic inflammatory disease (PID), potentially leading to subsequent infertility, ectopic pregnancy, and adverse pregnancy outcomes. There is growing evidence implicating infections (e.g., COVID-19, cytomegalovirus) in preeclampsia etiology, a maternal hypertensive disorder and leading cause of maternal morbidity and mortality. However, few studies have investigated the impact of STIs on preeclampsia risk. In this review, we provide an overview of the potential association between <i>C. trachomatis</i> and preeclampsia and identify future research needs through a critical evaluation of epidemiologic, in vitro, and in vivo studies. Unfortunately, current methodological limitations such as lower-quality study designs, selection bias, confounding bias, and variations in chlamydia diagnostic methods inhibit our understanding of the impact of <i>C. trachomatis</i> on preeclampsia. In addition, bench-side approaches such as animal models and in vitro studies have not elucidated the mechanisms linking <i>C. trachomatis</i> to preeclampsia. Understanding the biological pathways that could be disrupted by chlamydia is important as it may ultimately guide the development and use of novel therapeutics to augment standard antibiotic therapy to reduce pathology.</p>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"93 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal Neutrophil Inflammation Markers Predict Bronchopulmonary Dysplasia in Preeclamptic Pregnancies","authors":"Tifeng Xie,&nbsp;Liping Hong,&nbsp;Ruiting Yi,&nbsp;Yuxiang Cao,&nbsp;Feiyang Wang,&nbsp;Siying Fan,&nbsp;Yuan Li,&nbsp;Tao Liu,&nbsp;Peiwen Liu,&nbsp;Xinqi Zhong","doi":"10.1111/aji.70078","DOIUrl":"https://doi.org/10.1111/aji.70078","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Problem</h3>\u0000 \u0000 <p>Preeclampsia (PE) and bronchopulmonary dysplasia (BPD) are severe disorders that significantly affect maternal and neonatal health worldwide. This study evaluated the predictive value of maternal hematologic indicators in PE patients for the risk of offspring BPD.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method of Study</h3>\u0000 \u0000 <p>A retrospective cohort study was conducted enrolling infants born before 34 weeks’ gestation between September 2017 and December 2019 at the Third Affiliated Hospital of Guangzhou Medical University. Logistic regression analysis was used to evaluate the association between maternal hematologic indicators and offspring BPD. Subgroup analysis was performed to explore the interaction effects between maternal hematologic indicators and PE on neonatal BPD risk.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Data from 510 preterm infants and their mothers were analyzed. After adjusting for potential confounders, interaction effects between maternal white blood cell count (WBC), absolute neutrophil count (ANC), and PE on offspring BPD were observed (<i>p</i> for interaction &lt;0.05). Among normotensive mothers, elevated WBC or ANC did not significantly increase the risk of offspring BPD (OR [95% CI]: 1.02 [0.93–1.12] for both). In contrast, in PE patients, higher levels of WBC and ANC were independently associated with offspring BPD risk (OR [95% CI]: 1.24 [1.06–1.47] and 1.22 [1.05–1.44], respectively). Moreover, WBC &gt; 11.90 and NLR &gt; 7.65 in PE patients were identified as independent predictors of neonatal BPD (OR [95% CI]: 4.88 [1.27–21.04] and 4.67 [1.41–17.27], respectively).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Neutrophil-related hematologic indicators, including WBC, ANC, and NLR in PE patients, are significantly and independently associated with the development of BPD. These findings highlight the potential of neutrophils as a promising focus for investigating the relationship between these maternal and neonatal disorders.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"93 5","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Leukocyte Antigen Haplotypes Predisposing to Celiac Disease in Patients With Endometriosis","authors":"Silvia Vannuccini,&nbsp;Virginia Manzi,&nbsp;Mirko Tarocchi,&nbsp;Nico Donati,&nbsp;Francesco La Torre,&nbsp;Federico Toscano,&nbsp;Antonino Salvatore Calabrò,&nbsp;Felice Petraglia","doi":"10.1111/aji.70079","DOIUrl":"https://doi.org/10.1111/aji.70079","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Problem</h3>\u0000 \u0000 <p>Immunological abnormalities are well recognized in the pathogenesis of endometriosis and the co-existence of endometriosis with inflammatory bowel disease (IBD) and celiac disease (CD), along with other systemic immune disorders, is clinically relevant. Recent genetic studies revealed some shared genetic traits associated with the co-occurrence of endometriosis with different gastrointestinal or autoimmune disorders, highlighting common biological pathways. Since class II human leukocyte antigen (HLA) genes, HLA-DQ2 and -DQ8, show the strongest and best-characterized genetic susceptibility for CD, the present study aims to explore the presence of these haplotypes in non-celiac patients with endometriosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method of Study</h3>\u0000 \u0000 <p>A group of patients with endometriosis (<i>n</i> = 126) participated in the study and were compared to healthy women (<i>n</i> = 379), as controls. Subjects who were diagnosed with CD or who tested positive for CD antibodies were excluded. All patients and controls were genotyped for HLA haplotypes predisposing to CD (DQ2, DQ8). In the group of endometriosis patients who tested positive for DQ2 and/or DQ8, symptoms were also investigated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>At least one of the HLA-DQ2 and -DQ8 genotypes was detected in 43.3% of non-celiac endometriosis patients (OR: 1.82, 95% CI: 1.11–2.81), whereas 29.5% (<i>p</i> &lt; 0.01) of healthy women presented HLA haplotypes predisposing to CD. In endometriosis patients, no significant difference was shown between positive and negative in terms of endometriosis phenotype, or gynecological, and non-gynecological symptoms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our data revealed a significantly greater prevalence of predisposing haplotypes for CD in non-celiac patients with endometriosisthan in healthy subjects, suggesting that a common genetic background may explain the co-occurrence of endometriosis and CD.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"93 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aji.70079","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding a Potential Role for the NLRP3 Inflammasome in Placenta-Mediated Pregnancy Complications","authors":"Chloe G. Moss,&nbsp;Mark R. Dilworth,&nbsp;Lynda K. Harris,&nbsp;Sally Freeman,&nbsp;Alexander E. P. Heazell","doi":"10.1111/aji.70077","DOIUrl":"https://doi.org/10.1111/aji.70077","url":null,"abstract":"<p>Stillbirth affects approximately 2 million pregnancies annually and is closely linked to placental dysfunction, which may also present clinically as foetal growth restriction (FGR) or pre-eclampsia (PE). Placental dysfunction can arise from a range of insults, including the inflammatory conditions villitis of unknown aetiology (VUE) and chronic histiocytic intervillositis (CHI). Despite ample research regarding the pathophysiology of placental dysfunction, the literature surrounding placental inflammation is more limited, with no currently established treatments. In the absence of infection, placental inflammation is hypothesised to be stimulated by damage-associated molecular patterns (DAMPs), known as sterile inflammation. The NLRP3 inflammasome, a protein scaffold that unites within the cytosol of cells, is a proposed contributor. The NLRP3 inflammasome is dysregulated in numerous diseases and has shown evidence of activation through the sterile inflammatory pathway via DAMPs. Studies have demonstrated the upregulation of the NLRP3 inflammasome and its components in placentally-mediated pregnancy pathologies. However, the link between placental dysfunction seen in these disorders and the NLRP3 inflammasome is not yet firmly established. This manuscript aims to review the evidence regarding placental inflammation seen with placental dysfunction, discuss its association with the NLRP3 inflammasome, and identify potential therapeutic interventions for this pathological inflammatory response.</p>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"93 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aji.70077","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}