Decreased IL-33 Expression in the Cervix in LPS-Induced Preterm Birth and the Potential Role of Mast Cells: A Murine Model

IF 2.5 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology Pub Date : 2025-05-06 DOI:10.1111/aji.70085

Sema Avci, Ciler Celik-Ozenci, Nilay Kuscu, Nayce Ilayda Bektas

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引用次数: 0

Abstract

Problem

In order to gain a deeper understanding of the mechanisms underlying LPS-mediated preterm birth, it is crucial to investigate the relationship between preterm birth and mast cells (MCs). Moreover, the role of antihistamines in inflammatory processes during pregnancy remains incompletely understood.

Method of Study

CD-1 female mice were administered intrauterine lipopolysaccharide (LPS) via midline laparotomy to establish an inflammation-induced preterm birth model. The experimental groups (n = 6 per group) were formed as Nonpregnant and pregnant control, Sham, PBS, LPS, Cetirizine (CET) control, and two CET treatment groups (CET 10 mg/kg-low dose, and CET 20 mg/kg-high dose with LPS administration). Tissue samples were analyzed using immunohistochemistry and Western blot techniques.

Results

Our findings suggest that MCs play a significant role in preterm birth, with LPS administration inducing MC dysfunction in the reproductive tract during pregnancy. Additionally, high doses of CET may support inflammatory responses. A particularly notable result was the reduction in interleukin-33 (IL-33) expression in the cervix during LPS-induced preterm birth. This suggests that IL-33 may serve as a potential biomarker for preterm birth in the cervix.

Conclusions

The effects of CET during LPS-mediated preterm birth appear to be dose-dependent, warranting further exploration of their role in this context.

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lps诱导早产小鼠子宫颈IL-33表达降低及肥大细胞的潜在作用

为了更深入地了解脂多糖介导的早产机制，研究早产与肥大细胞（MCs）之间的关系至关重要。此外，抗组胺药在妊娠期炎症过程中的作用仍不完全清楚。研究方法采用剖腹中线切开腹腔注射脂多糖（LPS），建立炎症性早产小鼠模型。实验组（每组6只）分为未妊娠组、妊娠对照组、Sham、PBS、LPS、西替利嗪（CET）对照组和2个CET治疗组（CET 10 mg/kg低剂量、CET 20 mg/kg高剂量，LPS给药）。组织样本采用免疫组织化学和Western blot技术进行分析。结果研究结果表明，MCs在早产中起重要作用，LPS可诱导妊娠期生殖道MCs功能障碍。此外，高剂量的CET可能支持炎症反应。一个特别值得注意的结果是，在lps诱导的早产期间，子宫颈中白细胞介素-33 （IL-33）的表达减少。这表明IL-33可能作为子宫颈早产的潜在生物标志物。结论：在脂多糖介导的早产中，CET的作用似乎是剂量依赖性的，值得进一步探索其在此背景下的作用。

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来源期刊

American Journal of Reproductive Immunology 医学-免疫学

CiteScore

6.20

自引率

5.60%

发文量

314

审稿时长

2 months

期刊介绍： The American Journal of Reproductive Immunology is an international journal devoted to the presentation of current information in all areas relating to Reproductive Immunology. The journal is directed toward both the basic scientist and the clinician, covering the whole process of reproduction as affected by immunological processes. The journal covers a variety of subspecialty topics, including fertility immunology, pregnancy immunology, immunogenetics, mucosal immunology, immunocontraception, endometriosis, abortion, tumor immunology of the reproductive tract, autoantibodies, infectious disease of the reproductive tract, and technical news.