American Journal of Reproductive Immunology最新文献

{"title":"Comparative Analysis of Vaginal Inflammatory Cytokines Among HPV-Positive and HPV-Negative Women in Manila, Philippines","authors":"Domingo V. Macinas Jr.,&nbsp;Ourlad Alzeus G. Tantengco,&nbsp;Sheriah Laine M. de Paz-Silava,&nbsp;Ananth Kumar Kammala,&nbsp;Ramkumar Menon,&nbsp;Brandie DePaoli Taylor,&nbsp;Ian Kim B. Tabios","doi":"10.1111/aji.70148","DOIUrl":"https://doi.org/10.1111/aji.70148","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Problem</h3>\u0000 \u0000 <p>Human papillomavirus (HPV) is considered the necessary cause of cervical cancer. Studies showed that disruption of the inflammatory milieu in the cervicovaginal mucosa can promote the persistence of HPV, which can result in cervical carcinogenesis. This study determined sociodemographic factors and vaginal inflammatory cytokines associated with HPV infection in Manila, Philippines.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method</h3>\u0000 \u0000 <p>Cervicovaginal swab samples from 110 sexually active women, ages 21 and above, from Manila, diagnosed using real-time PCR, were selected for comparative expression analysis using sandwich-type ELISA of the cytokines IL-6, IL-8, IL-1b, IL-10, and TNF-α. Independent <i>t</i>-test, Wilcoxon Rank Sum, and Chi-square test were employed. Multivariable logistic regression was conducted to determine factors associated with HPV infection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Sociodemographic and behavioral factors analyzed in this study did not show a significant association with HPV infection. IL-8 was significantly lower (<i>p</i> &lt; 0.0001) while IL-10 was significantly higher (<i>p</i> &lt; 0.001) in the HPV-positive group compared to the HPV-negative samples. Multivariable logistic regression showed that higher IL-1b (OR: 1.007; 95% CI: 1.003–1.012) and lower IL-8 levels (OR: 0.979; 95% CI: 0.970–0.989) were associated with increased odds of HPV infection. IL-8 promotes neutrophil recruitment and viral clearance, IL-1β drives inflammation but may foster persistence via IL-17 induction, and IL-10 suppresses antiviral immunity, enabling HPV evasion.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study showed that lower IL-8 and higher IL-1β levels were independent predictors of HPV infection. These changes in the microenvironment may control immune cell infiltration and help establish HPV infection.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"94 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concerns Regarding Meta-Analyses Supporting ASRI's Corticosteroid Recommendations","authors":"","doi":"10.1111/aji.70153","DOIUrl":"https://doi.org/10.1111/aji.70153","url":null,"abstract":"","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"94 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commentary on “Initial and Longitudinal Trajectories in Hemoglobin Levels Predict the Prognosis of Patients With Premature Rupture of Membranes”","authors":"Tinghua Zhang","doi":"10.1111/aji.70157","DOIUrl":"https://doi.org/10.1111/aji.70157","url":null,"abstract":"","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"94 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"American Society of Reproduction Immunology Guideline on Recurrent Pregnancy Loss: Between Evidence-Based Practice and Excessive Medicalization","authors":"Melania Maria Ramos Amorim,&nbsp;Anna Catharina Carneiro da Cunha,&nbsp;Giordana Campos Braga,&nbsp;Lucas Félix Marinho Neves,&nbsp;Marina Amorim Albuquerque,&nbsp;Alexandre Delgado,&nbsp;Edson Borges de Souza,&nbsp;Leila Katz","doi":"10.1111/aji.70152","DOIUrl":"https://doi.org/10.1111/aji.70152","url":null,"abstract":"","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"94 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemokines and Their Association With Symptom Severity in Women With Endometriosis","authors":"Anton Sommar,&nbsp;Pinar Yalcin Bahat,&nbsp;Ipek Yildiz Özaydin,&nbsp;Eray Metin Güler,&nbsp;Marie Bixo,&nbsp;Torbjörn Bäckström,&nbsp;Engin Oral,&nbsp;Sahruh Turkmen","doi":"10.1111/aji.70156","DOIUrl":"https://doi.org/10.1111/aji.70156","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Problem</h3>\u0000 \u0000 <p>Various chemokines have been linked to endometriosis. Notably, chemokines such as CCL2, CXCL8, and CXCL1 have also been shown to promote nociception. In this study, we investigated whether increased serum concentrations and endometrial expression of chemokines (specifically CCL2, CXCL8, and CXCL1) are associated with heightened severity of pain symptoms in women with endometriosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method of Study</h3>\u0000 \u0000 <p>The study included women with endometriosis (with [<i>n</i> = 27] and without [<i>n</i> = 24] hormonal treatment) as well as healthy controls (<i>n</i> = 22). All participants underwent blood sampling and an endometrial biopsy during the secretory phase of the menstrual cycle. Symptom severity in the patient group was assessed using the pain dimension of the Endometriosis Health Profile 30 (EHP-30) and a visual analog scale (VAS) for pain.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Serum levels of CCL2 and CXCL1, as well as endometrial expression of CXCL8, were lower in women with endometriosis compared to controls. Furthermore, increased serum levels of CCL2, CXCL8, and CXCL1 were associated with higher EHP-30 pain domain scores in women with endometriosis. Similarly, elevated endometrial expression of CXCL8 and CXCL1 correlated with higher VAS scores. Notably, when the patient group was stratified based on ongoing hormonal treatment, CXCL1 emerged as the most promising target, with both increased serum concentration and endometrial expression consistently being associated with greater symptom severity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our results suggest that chemokines, particularly CXCL1, are associated with greater pain severity and reduced quality of life in women with endometriosis. However, these correlations do not establish causality and should be interpreted with caution.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"94 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aji.70156","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of MHC Class I Molecules (HLA-A, -B, -C, -E, -F, -G, and -J) Decreases From Early to Late Stage in Ovarian Cancer","authors":"Caglar Berkel","doi":"10.1111/aji.70154","DOIUrl":"https://doi.org/10.1111/aji.70154","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Problem</h3>\u0000 \u0000 <p>Interferon-ε (IFNε), which is highly abundant in the epithelium of the female reproductive tract (FRT), is a recently identified tumor suppressor for ovarian cancer. IFNε induces the expression of certain HLA class I family members in HGSOC (high-grade serous ovarian cancer), and its expression is lost during ovarian tumorigenesis. However, tumor stage–dependent expression of HLA class I family members in ovarian cancer has not been previously studied.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method of Study</h3>\u0000 \u0000 <p>Data analysis and visualization were performed using various gene expression and transcriptomics datasets in the R statistical programming environment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We found that the expression of HLA-A, -B, -C, -E, -F, -G, and -J is lower in late stage ovarian tumors compared to early-stage tumors. The total expression of HLA class I family members decreases with age in ovarian cancer. Furthermore, we showed that the expression of some IFN-regulated genes, which were shown to be upregulated by IFNε, decreases from early to late stage in ovarian cancer, in parallel to the loss of IFNε expression in ovarian tumorigenesis and possibly in tumor progression. We also found that breast tumors (another hormonally driven cancer) with positive progesterone receptor status have lower IFNε mRNA expression compared to those with negative PR status. Besides, we reported that breast tumors with positive estrogen receptor (ER) status have lower expression of IFNε compared to those with negative ER status.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Combined, this study points that the decrease in the expression of IFNε, HLAs, or some other IFNε-regulated genes during ovarian cancer progression might contribute to worse prognosis in advanced disease.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"94 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comments on “Effect of COVID-19 Infection During Pregnancy on the Plasma/Extracellular Vesicles Proinflammatory Cytokine Profile”","authors":"Renu Sah,&nbsp;Ankita Mathur,&nbsp;Venkata Dileep Kumar Veldi","doi":"10.1111/aji.70149","DOIUrl":"https://doi.org/10.1111/aji.70149","url":null,"abstract":"","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"94 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144929675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on “Outcomes of Ultimpro Testing Implementation for Patients With Unexplained Recurrent Implantation Failure: A Single-Center Retrospective Cohort Study”","authors":"Ranjana Sah,&nbsp;Rachana Mehta","doi":"10.1111/aji.70146","DOIUrl":"https://doi.org/10.1111/aji.70146","url":null,"abstract":"","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"94 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144910394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of Cytokines and Chemokines During the Peripartum Period in People Living With Human Immunodeficiency Virus","authors":"Jacqueline Corry,&nbsp;Natalia Zotova,&nbsp;Martine Tabala,&nbsp;Christina K. Cotrone,&nbsp;Fidéle Lumande Kasindi,&nbsp;Bienvenu Lebwaze Massamba,&nbsp;Pelagie Babakazo,&nbsp;Namal P. M. Liyanage,&nbsp;Nicholas T. Funderburg,&nbsp;Marcel Yotebieng,&nbsp;Jesse J. Kwiek","doi":"10.1111/aji.70147","DOIUrl":"https://doi.org/10.1111/aji.70147","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Problem</h3>\u0000 \u0000 <p>Pregnancy requires a precisely regulated immune response to support fetal development and minimize complications. Human immunodeficiency virus (HIV) infection induces a chronic inflammatory state and is associated with adverse pregnancy outcomes. In this observational cohort of pregnant people living with HIV in the Democratic Republic of the Congo (DRC), we sought to gain a deeper understanding of peripartum changes in cytokines, chemokines and soluble factors (collectively termed immune factors).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method of Study</h3>\u0000 \u0000 <p>Pregnant individuals living with HIV were enrolled during their second or third trimester in Kinshasa, DRC, between October 2020 and May 2021. Peripheral blood samples were collected at: enrollment (second or third trimester), 1–3 days postdelivery and postpartum. Concentrations of 45 immune factors were measured using LegendPlex and ELISAs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Most chemokines decreased significantly from enrollment to postdelivery, followed by a rebound in the postpartum period. Meanwhile, concentrations of myoglobin, serum amyloid A-1 protein (SAA), and interleukin 6 (IL-6), increased from enrollment to postdelivery, followed by a decrease postpartum. Finally, protein S100-A8/protein S100-A9 (S100A8/A9) and insulin-like growth factor-binding protein 4 (IGFBP4) consistently increased from enrollment through postpartum.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The postdelivery decline in chemokines observed in this study has not previously been reported. This shift may result from two mechanisms: greater-than-expected placental chemokine production or the degradation of key signaling molecules during parturition and early uterine involution. Additionally, the rise in proinflammatory markers from enrollment to postdelivery suggests a persistent inflammatory state, either unaffected by fetal delivery or worsened by tissue damage in the gestational parent.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"94 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aji.70147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Menstrual Effluent Derived Immune Cell Composition Is Distinct in Women Using Contraceptives: A Pilot Study","authors":"Aysel Gurbanova,&nbsp;Anne Stoverink,&nbsp;Imke M. B. van Wandeloo,&nbsp;Annemiek Nap,&nbsp;Nicole M. de Roos,&nbsp;Marien I. de Jonge,&nbsp;Janneke S. Hoogstad - van Evert,&nbsp;Renate G. van der Molen","doi":"10.1111/aji.70145","DOIUrl":"https://doi.org/10.1111/aji.70145","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Problem</h3>\u0000 \u0000 <p>Menstrual effluent (ME) is widely used to study immune-related reproductive disorders. However, women using contraceptives are often excluded from such studies due to limited knowledge of their impact on the endometrial immune cell composition. This gap in knowledge restricts our ability to include women using contraceptives in the scientific investigations and account for potential immunological variations associated with contraceptive use. Therefore, we aim to investigate whether contraceptive use is associated with differences in the uterine immune system.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method of Study</h3>\u0000 \u0000 <p>ME was collected from women using combined oral contraceptives (COC, <i>n</i> = 5), a copper intrauterine device (IUD, <i>n</i> = 3), or no contraceptives (<i>n</i> = 5). Immune cells were isolated from ME and analyzed using flow-cytometry. Data were analyzed using an unpaired <i>t</i>-test, followed by multiple testing correction using the false discovery rate (FDR) method.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Women using COCs exhibited a marked reduction in the frequency of the immunoregulatory CD56^brightCD16⁻ endometrial Natural Killer (eNK) cells compared to women using no contraceptives (30.1% ± 5.99 and 66.0% ± 10.6; <i>p</i> = 0.001). Additionally, women using copper IUD showed elevated frequencies of proliferating Ki-67⁺CD8⁺ T cells compared to COC users and controls (19.8% ± 4.3, 2.69% ± 1.23, and 3.77% ± 4.4).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This pilot study highlights the importance of considering contraceptive use when studying immune-related reproductive problems.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"94 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aji.70145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144888147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}