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Mikrobiologicheskii zhurnal最新文献

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[The comparative characteristics of Vibrio cholerae hemolysins]. [霍乱弧菌溶血素的比较特点]。
Mikrobiologicheskii zhurnal Pub Date : 1992-09-01
N R Telesmanich
{"title":"[The comparative characteristics of Vibrio cholerae hemolysins].","authors":"N R Telesmanich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Biochemical and biological properties of in vivo and in vitro hemolysins of cholera germs isolated by different authors as well as hemolysins of Vibrio parahaemolyticus and Escherichia coli have been comparatively characterized. According to the above data hemolysin of cholera germ El Tor is a thermolabile protein with molecular mass of 60-80 thou.; it is cytotoxic, enteropathogenic and lyses all the species of erythrocytes. Hemolysins of V. eltor and germs are immunologically related. Hemolysin of the cholera germ is thermostable and has molecular mass of 20-40 thou., that makes it similar to hemolysin of V. parahaemolyticus.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 5","pages":"105-12"},"PeriodicalIF":0.0,"publicationDate":"1992-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12624099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Current methods for the laboratory diagnosis of campylobacteriosis]. 【弯曲杆菌病实验室诊断的现行方法】。
Mikrobiologicheskii zhurnal Pub Date : 1992-07-01
D L Kirik, E A Shablovskaia, N A Chaĭka
{"title":"[Current methods for the laboratory diagnosis of campylobacteriosis].","authors":"D L Kirik,&nbsp;E A Shablovskaia,&nbsp;N A Chaĭka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An analytical review of recent publications of home and foreign authors on the problem of laboratory diagnosis of campylobacteriosis is presented. The commercial nutrient media, methods of creation of the microaerophilic conditions for cultivation of campylobacter are presented. The filtration method is preferable for isolation of these agents from the studied material highly contaminated by accompanying microflora. A special attention is paid to immunodiagnosis of campylobacteriosis: agglutination reaction, coagglutination reaction, passive hemagglutination reaction, immunoenzyme and radioimmune analyses. Seroepidemiological examination of the staff at one of meat-packing factories in the Republic carried out by the method of indirect immunoenzyme analysis has revealed high levels of anticampylobacteriosis antibodies in 17.9% of examinees. The promising trends in perfection of the methods for laboratory diagnosis of campylobacteriosis are outlined.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 4","pages":"100-6"},"PeriodicalIF":0.0,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12578068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The RNAse activity in mycoplasmas of different taxonomic positions]. [不同分类位置支原体RNAse活性]。
Mikrobiologicheskii zhurnal Pub Date : 1992-07-01
Z Ia Ianchinova
{"title":"[The RNAse activity in mycoplasmas of different taxonomic positions].","authors":"Z Ia Ianchinova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Dynamics of the RNAase accumulation in cells and culture fluid of strains of Acholeplasma and Mycoplasma genera has been studied. In certain strains of two genera of mollicutes some differences in dynamics of RNAase formation by them are determined.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 4","pages":"34-6"},"PeriodicalIF":0.0,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12579530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The intestinal microflora of persons subjected to a radiation lesion]. [受到辐射损害的人的肠道菌群]。
Mikrobiologicheskii zhurnal Pub Date : 1992-07-01
V I Sudenko, S S Nagornaia, L I Groma
{"title":"[The intestinal microflora of persons subjected to a radiation lesion].","authors":"V I Sudenko,&nbsp;S S Nagornaia,&nbsp;L I Groma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The content of large intestine has been studied in persons exposed to radiation injury in consequence of the accident at the Chernobyl Atomic Power Plant. It is stated that bifidobacteria (10(7)-10(10) cells in 1 g of feces) prevailed (as in healthy people), Bifidobacterium indicum being a dominating species. Amount of lactic-acid bacteria in 1 g of defecations of examined patients was within the range of 10(6)-10(9) cells and in certain persons it reached 10(10) cells (primarily fecal Enterococci). A considerable amount of patients with acute radiation sickness of the 3d degree had in their intestine 10(9)/g of lactic-acid bacteria, Lactobacillus casei and L. plantarum prevailing there. The frequency of yeast isolation from defecations of patients constituted 83%, while the number of cells in 1 g of feces--from 10 to 10(4). Yeast of the Candida genus, mainly Candida parapsilosis, prevailed. The species composition of isolated microorganisms has no substantial differences from microcenosis of healthy people. The content of intestine of persons suffered from radiation is characterized only by greater amount of lactic-acid bacteria and enterococci as compared with healthy adults.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 4","pages":"16-20"},"PeriodicalIF":0.0,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12579535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Bacteria of the genus Aeromonas and their role in aquaculture]. [气单胞菌属细菌及其在水产养殖中的作用]。
Mikrobiologicheskii zhurnal Pub Date : 1992-07-01
E V Kompanets, N M Isaeva, I A Balakhnin
{"title":"[Bacteria of the genus Aeromonas and their role in aquaculture].","authors":"E V Kompanets,&nbsp;N M Isaeva,&nbsp;I A Balakhnin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bacteria of genus Aeromonas are constant components of microbiota of fresh reservoirs where they, together with other microorganisms, play the part of natural biofilter and promote water self-purification. They are necessarily present in normal microflora of hydrobionts inhabiting fresh reservoirs. The greatest attention is paid by the researchers to Aeromonas and biotrophs in connection with epizootics in aquaculture which have become more frequent, in particular, under fish breeding. That is why the review is, to more extent, concerned in the works of this trend made by the foreign and home researchers for the last decade.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 4","pages":"89-99"},"PeriodicalIF":0.0,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12579538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The effect of physicochemical factors on the growth of Pseudomonas putida BS-2 on a medium with diethylene glycol]. [物化因素对恶臭假单胞菌BS-2在二甘醇培养基上生长的影响]。
Mikrobiologicheskii zhurnal Pub Date : 1992-07-01
S A Sedina
{"title":"[The effect of physicochemical factors on the growth of Pseudomonas putida BS-2 on a medium with diethylene glycol].","authors":"S A Sedina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The physicochemical factors of medium have been studied for their effect on the physiological indices of growth of Pseudomonas putida BS-2 culture utilizing diethylenglycol as the only source of carbon. Action of the supraoptimal temperature on the growth process of P. putida BS-2 is accompanied by a decrease (more than twice) in economic coefficient of substrate and specific growth rate as compared with their maximal values. Dependences of specific growth rate of P. putida BS-2 in the medium with diethylenglycol on the presence of NaCl in it within the range of its concentrations from 0 to 4% and methanol in the concentration range of 0-20 g/l follow the noncompetitive inhibition equation. When NaCl concentration in the medium is more than 4%, complete separation of constructive and energy metabolism processes is observed.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 4","pages":"43-8"},"PeriodicalIF":0.0,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12579532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The effect of physicochemical factors on the activity of DNA-dependent DNA polymerases in Acholeplasma laidlawii PG-8]. [理化因素对莱氏体PG-8 DNA依赖性DNA聚合酶活性的影响]。
Mikrobiologicheskii zhurnal Pub Date : 1992-07-01
S V Bezuglyĭ, I G Skripal', V V Babichev, L P Malinovskaia
{"title":"[The effect of physicochemical factors on the activity of DNA-dependent DNA polymerases in Acholeplasma laidlawii PG-8].","authors":"S V Bezuglyĭ,&nbsp;I G Skripal',&nbsp;V V Babichev,&nbsp;L P Malinovskaia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The biological and physico-chemical properties of DNA-dependent DNA-polymerases of Acholeplasma laidlawii PG-8 have been studied. The optimal parameters of maximal enzymatic activity are determined. It is stated that N-ethylmaleimide in concentration of 1 mM activated DNA-polymerase I by 52%, whereas DNA-polymerase II with reagent concentration of 0.5 mM demonstrated the peak of activity exceeding the control only by 10%. Spermidine in concentration of 1.5 mM for the first form of DNA-polymerase and 0.15 mM-for the second one increased the ability of both forms of polymerases to synthesize DNA by 10%. Aphidicolin added to the reaction medium up to concentration of 10 mg/ml decreased activity of forms I and II of enzymes by 83 and 68%, respectively. The presence of 0.6 mM of EDTA in the medium also negatively affected the activity of polymerases inhibiting it by 83% in form I and by 77%-in form II.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 4","pages":"21-7"},"PeriodicalIF":0.0,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12578069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The interaction of the causative agent of melioidosis with the host's alveolar macrophages]. [类鼻疽病原体与宿主肺泡巨噬细胞的相互作用]。
Mikrobiologicheskii zhurnal Pub Date : 1992-07-01
S F Popov, V Ia Kurilov, M P Lagutin
{"title":"[The interaction of the causative agent of melioidosis with the host's alveolar macrophages].","authors":"S F Popov,&nbsp;V Ia Kurilov,&nbsp;M P Lagutin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Studies were carried out on guinea pigs and albino rats, intranasally infected with P. pseudomallei C-141. The cells of bronchovesicular exudate were obtained from animals 1, 4 and 24 hours after infection. Electron microscopy was applied to study the process of interaction of the agent and alveolar macrophages. Bacteria were shown to form a capsule which permitted avoiding phagocytosis, when entering the host respiratory system. Microbes that failed to form a capsule were absorbed by macrophages and enclosed in a phagosome. Then some bacteria were destroyed by the lysosomal enzymes, the other synthesized a capsule, which protected them against the effect of phagolysosome content. There were also such microbes which escaped from a phagosome prior to fusion with lysosomes and parasitized in phagocytic cytoplasma forming a capsule there. By the end of the first 24 hours of observation the intact encapsulated microbe species were found to prevail in the host cells.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 4","pages":"3-7"},"PeriodicalIF":0.0,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12556100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The immunological shifts in the blood sera of persons inoculated with influenza vaccines]. [接种流感疫苗的人血清中的免疫变化]。
Mikrobiologicheskii zhurnal Pub Date : 1992-07-01
L D Stepanovskaia, R M Solomko, I M Avratinskiĭ, G P Katuntsevskaia, Zh I Kiriliuk, M P Iampol'skiĭ, E I Burdeniuk
{"title":"[The immunological shifts in the blood sera of persons inoculated with influenza vaccines].","authors":"L D Stepanovskaia,&nbsp;R M Solomko,&nbsp;I M Avratinskiĭ,&nbsp;G P Katuntsevskaia,&nbsp;Zh I Kiriliuk,&nbsp;M P Iampol'skiĭ,&nbsp;E I Burdeniuk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The dynamics of antihemagglutinin content in blood serum of students inoculated with different types of influenza virus vaccines has been traced out in the process of long-term observation. It is shown that this dynamics depends on the type of used vaccines, method of their introduction and epidemiological situation. It is stated that in the process of annual vaccination the highest titres of antihemagglutinins are observed in twice inoculated patients; after the third vaccination these titres decrease and after four-fold vaccination--they increase again.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 4","pages":"70-5"},"PeriodicalIF":0.0,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12579534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The surface structures and biological properties of Klebsiella strains]. [克雷伯氏菌菌株的表面结构和生物学特性]。
Mikrobiologicheskii zhurnal Pub Date : 1992-05-01
L D Martynenko, L P Zemliankina, G A Vilkov, A P Baturo
{"title":"[The surface structures and biological properties of Klebsiella strains].","authors":"L D Martynenko,&nbsp;L P Zemliankina,&nbsp;G A Vilkov,&nbsp;A P Baturo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The electron-microscopic method has been used to study the surface structures and biological properties (adhesiveness, antibiotic-resistance, biochemical properties) in 67 strains of Klebsiella isolated from one-year-old children with intestinal infections (56) and from healthy ones (11). It is found that bacterial cells in 58.2% of strains have fimbriae of the 3d type, in 20.9%--of the 1st type. No correlation has been revealed between the type of fimbriae and the studied properties.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 3","pages":"14-8"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12532762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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