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Mikrobiologicheskii zhurnal最新文献

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[Problems for discussion on the creation and use of bacterial preparations for the correction of the microflora of warm-blooded animals]. [关于制造和使用细菌制剂纠正温血动物微生物区系的讨论问题]。
Mikrobiologicheskii zhurnal Pub Date : 1992-11-01
V V Smirnov, S R Reznik, I B Sorokulova, V A V'iunitskaia
{"title":"[Problems for discussion on the creation and use of bacterial preparations for the correction of the microflora of warm-blooded animals].","authors":"V V Smirnov,&nbsp;S R Reznik,&nbsp;I B Sorokulova,&nbsp;V A V'iunitskaia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Different aspects of interrelation between macro- and microorganisms are considered in the paper. The phenomenon of rapid translocation of the exogenic microflora into blood and organs of the hot-blood animals opened by the authors is analyzed in detail. Strategy and tactics of application of biopreparations from living microbiol cultures are discussed as based on the authors' experimental data as well as on numerous data from literature.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 6","pages":"82-94"},"PeriodicalIF":0.0,"publicationDate":"1992-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12468064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The effect of the acid exopolysaccharide produced by the causative agent of melioidosis on macrophage function]. [类鼻疽病原体产生的酸外多糖对巨噬细胞功能的影响]。
Mikrobiologicheskii zhurnal Pub Date : 1992-11-01
V G Bozhko, I I Denisov, V S Rybkin, S F Popov, V P Vasil'ev
{"title":"[The effect of the acid exopolysaccharide produced by the causative agent of melioidosis on macrophage function].","authors":"V G Bozhko,&nbsp;I I Denisov,&nbsp;V S Rybkin,&nbsp;S F Popov,&nbsp;V P Vasil'ev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Acid exopolysaccharide of the melioidosis agent, isolated from the slime of alysogenic strain of Pseudomonas pseudomallei SVBDO-141 inhibits the capture of sheep erythrocytes--55Fe by mouse peritoneal macrophages. Administration of the sheep erythrocytes--55Fe, sensibilized by acid exopolysaccharide was proved not to have an antiphagocytic effect. Inhibition of phagocytic macrophagal activity depends on the conditions of isolation of acid exopolysaccharide.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 6","pages":"54-8"},"PeriodicalIF":0.0,"publicationDate":"1992-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12458781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A method for determining the toxigenicity of micromycetes]. 一种测定微菌毒力的方法。
Mikrobiologicheskii zhurnal Pub Date : 1992-11-01
N V Volkov
{"title":"[A method for determining the toxigenicity of micromycetes].","authors":"N V Volkov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method is suggested to determine toxigenicity of microscopic fungi from genera Aspergillus, Penicillium, Fusarium, etc. which contaminate fodder, food products and environment objects. The method is based on the property of micromycetes isolated from fodder, food products and environment objects to produce mycotoxins under optimal conditions of their cultivation on the nutrient media. Their further extraction by the organic solvent, evaporation and emulsification of the extract and its peroral introduction to young female rats cause a disease and death of animals. The suggested method may be used for recognizing and preventing mycotoxicoses of farm animals and poultry.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 6","pages":"78-81"},"PeriodicalIF":0.0,"publicationDate":"1992-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12468063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The agglutinability of cholera O-monoclonal immunoglobulins]. [霍乱o型单克隆免疫球蛋白的凝集性]。
Mikrobiologicheskii zhurnal Pub Date : 1992-11-01
L P Alekseeva, O S Burlakova, V V Balakhnova
{"title":"[The agglutinability of cholera O-monoclonal immunoglobulins].","authors":"L P Alekseeva,&nbsp;O S Burlakova,&nbsp;V V Balakhnova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A set of 10 monoclonal antibodies specific for vibrio species and of 5 monoclonal antibodies specific for serovar (Ogawa) was studied. These monoclonal antibodies were directed toward V. cholerae O1 antigen in agglutination reaction and on slide plates. Monoclonal antibodies agglutinating typical strains of cholera vibrios with titration range from 1: 6000 to 1: 25,000 were selected. MA were revealed to interact with cholera vibrio strains with reduced agglutinability. The set of agglutinating O monoclonal immunoglobulins was developed for laboratory identification of cholera O1 vibrios.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 6","pages":"50-4"},"PeriodicalIF":0.0,"publicationDate":"1992-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12458779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The isolation of ribosomes from Acholeplasma and the study of their physicochemical characteristics]. [血浆中核糖体的分离及其理化性质的研究]。
Mikrobiologicheskii zhurnal Pub Date : 1992-11-01
E S Korobkova, L P Panchenko, I G Skripal'
{"title":"[The isolation of ribosomes from Acholeplasma and the study of their physicochemical characteristics].","authors":"E S Korobkova,&nbsp;L P Panchenko,&nbsp;I G Skripal'","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Ribosomes are produced from Acholeplasma laidlawii PG-8 and A. laidlawii var. granulum str. 118. It is proved as possible to use the standard method of differential centrifugation for isolation of mollicute ribosomes. The physico-chemical properties of acholesplasma ribosomes are studied. The protein content for A. laidlawii PG-8 amounted to 39.6%, rRNA content--60.4% and for A. laidlawii var. granulum--39.1 and 60.8%, respectively. The RNA: protein ratio equals 1.5:1, the ratio of optic density indicates at 260 and 280 nm--2, that is peculiar to treated preparations of ribosomes. Sedimentation coefficient of acholeplasma ribosomes is 70S. The produced preparations of acholeplasma ribosomes can be used subsequently for creating the system of translation in vitro to study the biosynthesis processes of mollicute protein.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 6","pages":"41-5"},"PeriodicalIF":0.0,"publicationDate":"1992-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12458502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The status and outlook of the laboratory diagnosis of rotavirus infection]. 轮状病毒感染的实验室诊断现状与展望
Mikrobiologicheskii zhurnal Pub Date : 1992-11-01
V N Girin, I V Dziublik
{"title":"[The status and outlook of the laboratory diagnosis of rotavirus infection].","authors":"V N Girin,&nbsp;I V Dziublik","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Rotavirus infection is not only the most distributed disease of children but a widely spread cause of their death. Nowadays considerable methodical potential which permits effectively detecting the whole virions, group A, antigens of human rotaviruses, virus-specific RNA in the clinical material is accumulated. There is a tendency to an increase of sensitivity and expressivity of the methods based on the use of immunological principles for detection and calculation of the antigen-antibody complex. Molecular-biological methods of the study are developed, which is important for the theory and practice of this infection control.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 6","pages":"95-102"},"PeriodicalIF":0.0,"publicationDate":"1992-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12511291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The cloning of fragments of the streptomycete plasmid pSG1912 as a part of the vector pUC19]. 链霉菌质粒pSG1912片段作为载体pUC19的一部分的克隆。
Mikrobiologicheskii zhurnal Pub Date : 1992-09-01
V B Zaverukha, B V Polevoda, L V Polishchuk, B P Matseliukh
{"title":"[The cloning of fragments of the streptomycete plasmid pSG1912 as a part of the vector pUC19].","authors":"V B Zaverukha,&nbsp;B V Polevoda,&nbsp;L V Polishchuk,&nbsp;B P Matseliukh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>DNA fragments of plasmid pSG1912 isolated from Streptomyces globisporus 1912 have been cloned into vector pUC19 in the E. coli cells. Stability of inheritance of hybrid derivatives in enterobacteria has been studied. The clones where recombinant plasmids are stably inherited have been chosen. The detailed restriction maps of cloned DNA fragments of pSG1912 have been constructed.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 5","pages":"30-5"},"PeriodicalIF":0.0,"publicationDate":"1992-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12624098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The effect of a Coxsackie viral infection on the chromosomal apparatus of the cells of fetuses and newborn mice]. 柯萨奇病毒感染对胎儿和新生小鼠细胞染色体结构的影响。
Mikrobiologicheskii zhurnal Pub Date : 1992-09-01
L V Pavlenko, A I Evtushenko, V P Shirobokov
{"title":"[The effect of a Coxsackie viral infection on the chromosomal apparatus of the cells of fetuses and newborn mice].","authors":"L V Pavlenko,&nbsp;A I Evtushenko,&nbsp;V P Shirobokov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Coxsackie virus B1 has been studied for its effect on the chromosome apparatus in cells of mouse feti and newborn mice in case of intraperitoneal infection of females on the 3d week of pregnancy. It is stated that the virus readily moves across the placental barrier and induces chromosome aberrations in the cells of feti, their number being 2.5 times higher than the control level. The maximal number of structural chromosome aberrations was registered during the first three days from the moment of infecting. Then a gradual decrease in the number of aberrant metaphases in the cells of newborn mice was observed. It had reached the value of the control level by the 17th day of observation.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 5","pages":"59-62"},"PeriodicalIF":0.0,"publicationDate":"1992-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12507543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The kinetics of glycol destruction by a Pseudomonas putida BS-2 strain]. [恶臭假单胞菌BS-2菌株破坏乙二醇的动力学]。
Mikrobiologicheskii zhurnal Pub Date : 1992-09-01
S A Sedina
{"title":"[The kinetics of glycol destruction by a Pseudomonas putida BS-2 strain].","authors":"S A Sedina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Destruction of ethylene glycol and diethylene glycol by Pseudomonas putida BS-2 culture under conditions of its batch cultivation has been studied for its physiological regularities. The specific rate of the biomass growth in the region of limiting substrate concentrations depends on the diethylene glycol concentration in the medium and follows the Mono equation. A semisaturation constant for diethylene glycol is 209 +/- 17 mg/d. The specific rate of the culture growth is independent of the ethylene glycol concentration in the medium within a wide range from 0.08 to 10 g/l. Kinetics of the bacteria growth inhibition by excess of substrates is a complex character and obeys none of the known models of the substrate inhibition.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 5","pages":"53-9"},"PeriodicalIF":0.0,"publicationDate":"1992-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12624103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The properties of cell wall hydrolysates of Streptococcus group A]. [A群链球菌细胞壁水解物的性质]。
Mikrobiologicheskii zhurnal Pub Date : 1992-09-01
Z F Shmakovoa, V D Kuznetsov, F R Serna, O P Degtereva
{"title":"[The properties of cell wall hydrolysates of Streptococcus group A].","authors":"Z F Shmakovoa,&nbsp;V D Kuznetsov,&nbsp;F R Serna,&nbsp;O P Degtereva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Products obtained from lysis in the cell wall of group A streptococcus have been studied in different growth phases: at the end of the exponential phase and in the stationary one. Endo-beta-N-acetylmuramidase extracted from the culture liquid of Streptomyces levoris 96 has been used for lysis of streptococcus. It is stated that streptococcus cell walls isolated at different growth stages differ in the protein and polysaccharide content. High content of protein in the cell wall of a young culture makes lower the initial rate of the walls' hydrolysis by endo-beta-N-acetylmuramidase. However, with the enzyme penetration into peptidoglycan the rate of hydrolysis of cell walls gets higher and after four-hour incubation the lysis degree of walls of the 16- and 8-hour cultures reaches the equal value (63%). Studies in the protein composition of lysates of the streptococcus cell walls have shown that they contain at least 12 proteins most of which are acid and neutral ones.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 5","pages":"35-41"},"PeriodicalIF":0.0,"publicationDate":"1992-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12624100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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