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Mikrobiologicheskii zhurnal最新文献

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[The use of the Soviet detergents dezintegron-O and dezintegron-B for the solubilization of influenza virus glycoproteins]. [苏联洗涤剂dezintegron-O和dezintegron-B对流感病毒糖蛋白的增溶作用]。
Mikrobiologicheskii zhurnal Pub Date : 1991-05-01
Iu L Krivorutchenko, Iu S Krivoshein, I B Andronovskaia, L N Kastrikina, N I Lonskaia, A P Rud'ko
{"title":"[The use of the Soviet detergents dezintegron-O and dezintegron-B for the solubilization of influenza virus glycoproteins].","authors":"Iu L Krivorutchenko,&nbsp;Iu S Krivoshein,&nbsp;I B Andronovskaia,&nbsp;L N Kastrikina,&nbsp;N I Lonskaia,&nbsp;A P Rud'ko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Native detergents, desintegron-O-and desintegron-B, solubilize hemagglutinins of the influenza virus as intensively, as foreign drugs (mulgophen, zwittergent and sodium sarcozyl) do. This permits recommending desintegrons for quantitative determination of hemagglutinin of the influenza virus in virus-containing materials by means of the reaction of single radial immunodiffusion. Desintegron-O and desintegron-B permit extracting enzymatically active neuraminidase from influenza A viruses with 44.6-83.4% yield.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 3","pages":"96-100"},"PeriodicalIF":0.0,"publicationDate":"1991-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12942758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A method for selecting Streptomyces spheroides mutants--producers of an exoprotease with fibrinolytic activity]. [一种选择球形链霉菌突变体的方法-具有纤溶活性的外蛋白酶的生产者]。
Mikrobiologicheskii zhurnal Pub Date : 1991-05-01
N I Prianishnikova, M A Al'-Nuri
{"title":"[A method for selecting Streptomyces spheroides mutants--producers of an exoprotease with fibrinolytic activity].","authors":"N I Prianishnikova,&nbsp;M A Al'-Nuri","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The paper present a description of a fast method to obtain mutants of Streptomyces spheroides--supersynthetics of exoprotease. The primary culture spores being irradiated by UV-rays, the mutants are formed with presence in the cultural medium of own proteolytic enzyme added from without. The described method is based on the phenomenon of growth suppression in the primary culture spore by high concentrations of the own proteolytic enzyme. It permits selecting producers with high proteolytic activity of fibrinolytic proteases from the small number of mutants grown in these colonies.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 3","pages":"54-6"},"PeriodicalIF":0.0,"publicationDate":"1991-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12942754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The action of bacillary inhibitory substances on human-pathogenic, phytopathogenic and saprotrophic fungi]. [细菌抑制物质对人致病性、植物致病性和腐坏性真菌的作用]。
Mikrobiologicheskii zhurnal Pub Date : 1991-05-01
A I Shelokhovich, V S Lesovoĭ, P M Lashchenov
{"title":"[The action of bacillary inhibitory substances on human-pathogenic, phytopathogenic and saprotrophic fungi].","authors":"A I Shelokhovich,&nbsp;V S Lesovoĭ,&nbsp;P M Lashchenov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The study has shown that the growth of many soil fungi is inhibited by the effect of substances which are produced into the environment by some bacilli-saprotrophs. Besides, these substances can bring about a dimorphism of the Trichoderma, Blastomyces, Emmonsia, Alternaria and Verticillium fungi from the mycelial form into the yeast form, which results in the death of the fungi. The existence of bacilli capable of producing the inhibitory substances, in the soil in the territory of the USSR may explain, to a certain extent, the absence of the coccidioidomycosis and North American blastomycosis agents pathogenic for people and many animals.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 3","pages":"21-4"},"PeriodicalIF":0.0,"publicationDate":"1991-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12942235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The microflora in suppurative-catarrhal endometritis in cattle]. [牛化脓性卡他性子宫内膜炎的微生物群]。
Mikrobiologicheskii zhurnal Pub Date : 1991-03-01
V A Kudriavtsev, L A Safronova, I A Kozachko, A I Osadchaia, V I Liubetskiĭ, S K Iukhimchuk, V P Polishchuk
{"title":"[The microflora in suppurative-catarrhal endometritis in cattle].","authors":"V A Kudriavtsev,&nbsp;L A Safronova,&nbsp;I A Kozachko,&nbsp;A I Osadchaia,&nbsp;V I Liubetskiĭ,&nbsp;S K Iukhimchuk,&nbsp;V P Polishchuk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Microflora isolated from cattle with acute postnatal pus-catarrhal endometritis has been studied. It is shown that the given pathology is caused by associations of different bacterial species. 284 strains isolated from the uterus content of 63 sick animals have been identified, their properties being studied. Pathogenic gram-negative bacilli and gram-positive pus-producing cocci are responsible for the studied pathology. Considerable inoculation capacity has been established for conditionally pathogenic microorganisms of different species and genera whose role in pathogenesis is to be found out.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 2","pages":"3-9"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13021400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Induced bacterial chemiluminescence]. [诱导细菌化学发光]。
Mikrobiologicheskii zhurnal Pub Date : 1991-03-01
N I Zhiliaev, A E Franchuk, S I Sytnik, A A Vorontsov, I B Sen'kov
{"title":"[Induced bacterial chemiluminescence].","authors":"N I Zhiliaev,&nbsp;A E Franchuk,&nbsp;S I Sytnik,&nbsp;A A Vorontsov,&nbsp;I B Sen'kov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Oxygen-peroxide-induced superweak luminescence of some microorganisms has been studied by means of the native chemiluminometer KhLM1Ts-01. It is established that total chemiluminescence decrease in the bacteria as follows: Escherichia coli----Bacillus subtilis----Proteus vulgaris----Bacillus mycoides----B. licheniformis----Pseudomonas aeruginosa----Staphylococcus epidermidis----S. aureus----Serratia marcescens----Salmonella enteritidis. A hypothesis is put forward on a possible mechanisms of appearance of the superweak luminescence of bacteria and expediency to use chemoluminometry to differentiate microorganisms.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 2","pages":"59-62"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13022154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The characteristics of glucose consumption by an L-929 cell culture infected with Chlamydia]. [感染衣原体的L-929细胞培养物的葡萄糖消耗特征]。
Mikrobiologicheskii zhurnal Pub Date : 1991-03-01
I I Mavrov, M S Goncharenko, E V Shchegoleva, I A Chernobaeva, L M Pustovoĭtova
{"title":"[The characteristics of glucose consumption by an L-929 cell culture infected with Chlamydia].","authors":"I I Mavrov,&nbsp;M S Goncharenko,&nbsp;E V Shchegoleva,&nbsp;I A Chernobaeva,&nbsp;L M Pustovoĭtova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of the intracellular parasite Chlamydia trachomatis on the host cell energy metabolism has been studied. Glucose consumption by L-929 cell cultures infected or uninfected by C. trachomatis was studied in comparison during a 3-days cultivation. The content of glucose in the cultural medium was determined every 5, 24, 48, 72 hrs according to the developmental cycle of the parasite. It was shown that cell infection by C. trachomatis induced the alteration of energy metabolism via an increase in the glucose consumption rate.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 2","pages":"95-8"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13022157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The thermodynamic parameters of the conformational transitions in Mycoplasma DNA]. 支原体DNA构象转变的热力学参数。
Mikrobiologicheskii zhurnal Pub Date : 1991-03-01
L P Panchenko, V A Turchin, I G Skripal'
{"title":"[The thermodynamic parameters of the conformational transitions in Mycoplasma DNA].","authors":"L P Panchenko,&nbsp;V A Turchin,&nbsp;I G Skripal'","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>DNA preparations have been isolated from 10 strains of phytopathogenic mycoplasms and collection culture Achole plasma laidlawii PG-8. Thermodynamic parameters of denaturation changes in the secondary structure (transconformation) of DNA of these mycoplasms have been determined. It is shown that denaturation temperature is 82.3-83.1 degree C and enthalpy of conformational DNA transitions calculated per 1 g of dry substance is 56.2-61.9 J/g. Changes in the enthalpy (delta H) and entropy (delta S) calculated per 1 mol of nucleotide pairs varied in the range of 35.6-39.0 J/m.p. and 995-109.6 J degree m.p. respectively. No linear dependence of transconformational thermal adsorption on the temperature of DNA denaturation of the studied strains of mycoplasms are revealed, which is probably connected with structural peculiarities of DNA of these microorganisms.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 2","pages":"20-3"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13022149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The adhesion of microorganisms to metal-containing carbon sorbents]. [微生物对含金属碳吸附剂的粘附]。
Mikrobiologicheskii zhurnal Pub Date : 1991-03-01
A V Grigor'ev, V A Znamenskiĭ, V I Bugaev, M E Shor-Chudnovskiĭ, N T Kartel'
{"title":"[The adhesion of microorganisms to metal-containing carbon sorbents].","authors":"A V Grigor'ev,&nbsp;V A Znamenskiĭ,&nbsp;V I Bugaev,&nbsp;M E Shor-Chudnovskiĭ,&nbsp;N T Kartel'","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 2","pages":"98-103"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13068121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[2 site-specific endonucleases of the cyanobacterium Nostoc linckia]. [2位点特异性内切酶蓝藻Nostoc linckia]。
Mikrobiologicheskii zhurnal Pub Date : 1991-03-01
A I Mel'nik, B A Rebentish, A V Bolotin, M I Mendzhul
{"title":"[2 site-specific endonucleases of the cyanobacterium Nostoc linckia].","authors":"A I Mel'nik,&nbsp;B A Rebentish,&nbsp;A V Bolotin,&nbsp;M I Mendzhul","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two restrictases Nli387/7 I and Nli387/7 II have been isolated from cyanobacterium Nostoc linckia using chromatography on phosphocellulose, \"Mono Q\" column, and heparin sepharose 4B. The preparations are described by the method of electrophoresis in polyacrylamide gel under denaturing conditions. Catalytic properties of restrictases are determined: optimal pH of the action--9.0--9.5, optimal concentration of Na+--5 mM, that of Mg2+--6 mM, optimal temperature--37 degrees C. The isolated enzymes are isoschizomers of restrictases avaI and AvaII. The point of cutting is determined for enzyme Nli387/7 I. It is shown that restrictase Nli387/7 I is a false isoschizomer Ava I.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 2","pages":"24-8"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12816702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The effect of dispersed silicon dioxide--aerosil A-300--on the growth of Saccharomyces cerevisiae yeasts]. [分散二氧化硅A-300对酿酒酵母菌生长的影响]。
Mikrobiologicheskii zhurnal Pub Date : 1991-03-01
I K Kurdish, V L Bikhunov, E A Tsimberg, S V El'chits, E L Vygovskaia, A A Chuĭko
{"title":"[The effect of dispersed silicon dioxide--aerosil A-300--on the growth of Saccharomyces cerevisiae yeasts].","authors":"I K Kurdish,&nbsp;V L Bikhunov,&nbsp;E A Tsimberg,&nbsp;S V El'chits,&nbsp;E L Vygovskaia,&nbsp;A A Chuĭko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>High-dispersed silicon dioxide--aerosil A-300 has been studied for its effect on the growth of yeast Saccharomyces cerevisiae. It is shown that at heterophase cultivation of these microorganisms under periodical conditions introduction of aerosil A-300 induces a decrease of the lag phase duration and an increase of the maximum specific growth rate and economic coefficient of the carbon substrate utilization. This high-dispersed material has exerted the similar effect under the conditions of chemostatic cultivation. Optimal parameters of this process are determined.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 2","pages":"41-4"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12815527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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