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[The effect of the antibiotic AL-87 on the amino acid yield from Staphylococcus aureus 209P cells]. [抗生素AL-87对金黄色葡萄球菌209P细胞氨基酸产量的影响]。
Mikrobiologicheskii zhurnal Pub Date : 1991-09-01
V V Snirnov, L N Churkina, E A Kiprianova, L I Pustovalova
{"title":"[The effect of the antibiotic AL-87 on the amino acid yield from Staphylococcus aureus 209P cells].","authors":"V V Snirnov,&nbsp;L N Churkina,&nbsp;E A Kiprianova,&nbsp;L I Pustovalova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Antibiotic AL-87 has been studied for its effect on the composition of intracellular free amino acids and of amino acids in culture fluid of Staphylococcus aureus 209 P. It is established that the content of amino acids in the culture fluid of S. aureus 209 P is doubled due to antibiotics, while the content of intracellular free amino acids considerably decreases. Spectrum of free amino acids of S. aureus 209 P is presented by 17 basic amino acids. When there is a sub-bacteriostatic concentration of the antibiotic in the medium all free amino acids tend to leave the cells, the content of aspartic acid, serine, threonine and leucine in the medium being increased. Data obtained when studying the effect of antibiotic AL-87 on the composition of free amino acids of Staphylococcus agree well with the previously obtained results from the study of the fatty acid composition of cells. In the light of these data it may be supposed that an increase of the membrane permeability and as a result of it an outlet of amino acids into the medium is one of constituents of the mechanism of antibiotic AL-87 action on Staphylococcus cells.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 5","pages":"37-40"},"PeriodicalIF":0.0,"publicationDate":"1991-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12953508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The isolation and partial purification of 2 DNA-dependent DNA polymerases from Acholeplasma laidlawii PG-8]. [2个DNA依赖性DNA聚合酶的分离和部分纯化]。
Mikrobiologicheskii zhurnal Pub Date : 1991-09-01
S V Bezuglyĭ, V V Babichev, I G Skripal', L P Malinovskaia
{"title":"[The isolation and partial purification of 2 DNA-dependent DNA polymerases from Acholeplasma laidlawii PG-8].","authors":"S V Bezuglyĭ,&nbsp;V V Babichev,&nbsp;I G Skripal',&nbsp;L P Malinovskaia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two forms of DNA-dependent DNA-polymerase have been isolated and partially purified from the limited amount of biomass of cells Acholeplasma laidlawii PG-8, a typical representative of genus Acholeplasmataceae, as a result of successive chromatography on the columns with DEAE-cellulose DE-52 and Green A-sepharose. The first form of DNA-polymerase is eluted from the ion-exchange column with NaCl concentration of 0.1 M from the column with Green A-sepharose of 0.27 M, while the second form-with NaCl concentrations of 0.6 and 0.4 M, respectively. The both enzymatic activities are able to implement DNA synthesis. The conditions of DNA-polymerase production proved to be rather convenient for isolation of the concentrated and highly active enzymes.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 5","pages":"21-6"},"PeriodicalIF":0.0,"publicationDate":"1991-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12953510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The interaction of alkylating derivatives of oligodeoxyribonucleotides and their methylphosphonate analogs with Mycoplasma cells]. [低聚脱氧核糖核苷酸的烷基化衍生物及其甲基膦酸类似物与支原体细胞的相互作用]。
Mikrobiologicheskii zhurnal Pub Date : 1991-07-01
L P Panchenko, O V Egorov, A S Raĭt, E M Ivanova, N V Amirkhanov, V F Zarytova, V V Vlasov, I G Skripal'
{"title":"[The interaction of alkylating derivatives of oligodeoxyribonucleotides and their methylphosphonate analogs with Mycoplasma cells].","authors":"L P Panchenko,&nbsp;O V Egorov,&nbsp;A S Raĭt,&nbsp;E M Ivanova,&nbsp;N V Amirkhanov,&nbsp;V F Zarytova,&nbsp;V V Vlasov,&nbsp;I G Skripal'","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Alkylating derivatives of decathymidylates and methylphosphonate analogs of oligodeoxyribonucleotides (MPAO) were studied for their interaction with cells of Acholeplasma laidlawii PG-8, Mycoplasma capricolum California Kid, M. pneumoniae FH and phytopathogenic strain (St. 118). It is shown that MPAO of octa- and hexadecathymidylates as well as decathymidylates 3'-terminal modified by phenazine and cholesterol groupings are sorbed by mycoplasma cells and can penetrate inside the cells. Efficiency of binding of alkylating derivatives and MPAO with mycoplasma cells depends on interaction time of reagents, their concentration in the reaction mixture and temperature.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 4","pages":"63-8"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12917130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The stability of the alkylating derivatives of oligodeoxyribonucleotides containing a cholesterol or phenazine radical added to the 3'-termination during their interaction with Acholeplasma laidlawii PG-8]. [含有胆固醇或非那嗪自由基的低聚脱氧核糖核苷酸的烷基化衍生物在与laidlawii PG-8相互作用时的稳定性]。
Mikrobiologicheskii zhurnal Pub Date : 1991-07-01
L P Panchenko, O V Egorov, I G Skripal', A S Raĭt, E M Ivanova, V F Zarytova, V V Vlasov
{"title":"[The stability of the alkylating derivatives of oligodeoxyribonucleotides containing a cholesterol or phenazine radical added to the 3'-termination during their interaction with Acholeplasma laidlawii PG-8].","authors":"L P Panchenko,&nbsp;O V Egorov,&nbsp;I G Skripal',&nbsp;A S Raĭt,&nbsp;E M Ivanova,&nbsp;V F Zarytova,&nbsp;V V Vlasov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Stability of alkylating derivatives of decathymidylates protected on the 3'-terminal by cholesterol and phenazine residues has been studied in the process of their interaction with cells of Acholeplasma laidlawii PG-8. It is shown that the studied reagents are not split by nucleases of A. laidlawii PG-8 for the time necessary for alkylation of mycoplasma biopolymers.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 4","pages":"58-63"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12917129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Complexes of soil micromycetes in the area of the influence of the Chernobyl Atomic Electric Power Station]. [切尔诺贝利核电站影响地区土壤微菌复合体]。
Mikrobiologicheskii zhurnal Pub Date : 1991-07-01
N N Zhdanova, A I Vasilevskaia, L V Artyshkova, V I Gavriliuk, T N Lashko, Iu S Sadovnikov
{"title":"[Complexes of soil micromycetes in the area of the influence of the Chernobyl Atomic Electric Power Station].","authors":"N N Zhdanova,&nbsp;A I Vasilevskaia,&nbsp;L V Artyshkova,&nbsp;V I Gavriliuk,&nbsp;T N Lashko,&nbsp;Iu S Sadovnikov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Complexes of soil micromycetes in the Chernobyl 30-km zone of the Ukrainian Polesye were studied for 1986-1989 with regard for such ecological parameters as the level of radiation contamination, a particular observation site, depth of soil horizon and season. As a result of the study correlation pleiads of soil micromycete complexes have been revealed with their structure and fungal genera characteristic of such complexes determined. The overwhelming majority of correlation pleiads of fungal complexes are attributed to complex-organized ones and this indicated high radioresistance of mycobiota in the studied, soils. Melanine-containing genera of fungi rank among the first in formation of correlation pleiads of soil micromycete complexes.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 4","pages":"3-9"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12917128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The interaction of soil micromycetes with "hot" particles in a model system]. [模型系统中土壤微菌与“热”粒子的相互作用]。
Mikrobiologicheskii zhurnal Pub Date : 1991-07-01
N N Zhdanova, T N Lashko, T I Redchits, A I Vasilevskaia, L G Borisiuk, O I Siniavskaia, V I Gavriliuk, P N Muzalev
{"title":"[The interaction of soil micromycetes with \"hot\" particles in a model system].","authors":"N N Zhdanova,&nbsp;T N Lashko,&nbsp;T I Redchits,&nbsp;A I Vasilevskaia,&nbsp;L G Borisiuk,&nbsp;O I Siniavskaia,&nbsp;V I Gavriliuk,&nbsp;P N Muzalev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A model system which permits observing for a long time and fixing interaction of fungi with a radiation source has been created on the basis of an isolated \"hot\" particle, deficient mineral medium (saccharose content 60 mg/l) and suspension of fungal conidia. Five species (six strains) of micromycetes isolated from radionuclide-contaminated soils and fifteen \"hot\" particles have been tested. It has been found out for the first time that Cladosporium cladosporioides and Penicillium roseo-purpureum are able actively overgrow \"hot\" particles whose radioactivity did not exceed 3.1-1.0(-7) Ci by gamma-spectrum and to destroy them 50-150 days later. Certain changes in morphology of fungi-destructors of \"hot\" particles are revealed. A problem on ecological significance of the found phenomenon is discussed.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 4","pages":"9-17"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12917132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The selection of the composition of the medium for optimizing the amine-synthetic activity of aerobic bacilli]. [优化好氧杆菌胺合成活性的培养基组成的选择]。
Mikrobiologicheskii zhurnal Pub Date : 1991-07-01
V A Kudriavtsev, L A Safronova, A I Osadchaia, I A Kozachko
{"title":"[The selection of the composition of the medium for optimizing the amine-synthetic activity of aerobic bacilli].","authors":"V A Kudriavtsev,&nbsp;L A Safronova,&nbsp;A I Osadchaia,&nbsp;I A Kozachko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Nutrient medium chosen as a basic one after preliminary test of several media known from literature has been optimized to intensify biosynthesis and amine nitrogen production by three strains of aerobic sporulating bacteria to culture liquid. The method of mathematical planning used in the experiments has permitted obtaining the components ratio for the medium on which production of amine nitrogen to the environment increased 2.3-3.2 times. The best variants of the optimized medium promoted an increase of the aminosynthetic activity of the studied bacteria by more than 320%. The obtained nutrient medium is appropriate for a wide screening of aerobic bacilli for their ability to synthesize amino compounds.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 4","pages":"68-73"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12917131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The flotation characteristics of Bacillus cells and spores]. 芽孢杆菌细胞和孢子的浮选特性。
Mikrobiologicheskii zhurnal Pub Date : 1991-05-01
E V Stabnikova, N N Gregirchak, T O Taranenko
{"title":"[The flotation characteristics of Bacillus cells and spores].","authors":"E V Stabnikova,&nbsp;N N Gregirchak,&nbsp;T O Taranenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Variations in hydrophobicity of the surface of bacillary cells and their capacity to flotation in the process of batch cultivation have been studied. It is shown that hydrophobicity of the cell surface increases in the course of batch cultivation of Bacillus thuringiensis, B. licheniformis and B. megaterium. Hydrophobicity of spores of the mentioned cultures is considerably higher than that of the vegetative cells. The increase of hydrophobicity of bacillary cells positively correlated with their capacity to flotation. That is why the use of flotation for the age fractionation of bacillary cells is possible: spores are concentrated in the foam while vegetative cells remain in the culture liquid.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 3","pages":"38-42"},"PeriodicalIF":0.0,"publicationDate":"1991-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12942236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Dimorphism in the fungus Coccidioides immitis Rixford et Gilchrist under the action of saprophytic bacilli]. [腐生杆菌作用下真菌球虫的二态性]。
Mikrobiologicheskii zhurnal Pub Date : 1991-05-01
A I Shelokhovich, V S Lesovoĭ, A V Lipnitskiĭ
{"title":"[Dimorphism in the fungus Coccidioides immitis Rixford et Gilchrist under the action of saprophytic bacilli].","authors":"A I Shelokhovich,&nbsp;V S Lesovoĭ,&nbsp;A V Lipnitskiĭ","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It is shown that there are specific substances produced and secreted into the environment by saprotrophic bacilli. These inhibit the growth of the coccidioidal fungus in its mycelial form and some cells are converted into the yeast form, which leads to the destruction of the fungi (in natural environment) or, if the conditions allow, to their growth in the yeast form. This phenomenon, existence of a large amount of bacilli antagonistic to Coccidioides immitis, may be one of reasons why the latter has not been isolated so far from the soil in the territory of the USSR.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 3","pages":"17-20"},"PeriodicalIF":0.0,"publicationDate":"1991-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12942752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The immunochemical characteristics of the lipopolysaccharides of Pseudomonas syringae (pathovars atrofaciens and phaseolicola) and P. holci (serogroup VI)]. [丁香假单胞菌(atrofaciens病原菌和phaseolicola病原菌)和霍氏假单胞菌(P. holci，血清组VI)脂多糖的免疫化学特征]。
Mikrobiologicheskii zhurnal Pub Date : 1991-05-01
L M Iakovleva, G M Zdorovenko, N Ia Gubanova, R I Gvozdiak
{"title":"[The immunochemical characteristics of the lipopolysaccharides of Pseudomonas syringae (pathovars atrofaciens and phaseolicola) and P. holci (serogroup VI)].","authors":"L M Iakovleva,&nbsp;G M Zdorovenko,&nbsp;N Ia Gubanova,&nbsp;R I Gvozdiak","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Lipopolysaccharides (LPS) of the representatives of strains of serogroup VI Pseudomonas syringae (P. syringae pv. atrofaciens 2399, pv. phaseolicola 120a, 7842 and P. holci 8299) possessing virulence and confinement to the host-plant are characterized by high serological activity in direct and cross reactions of the binary diffusion in agar, immunoelectrophoresis, passive hemagglutination and inhibition of passive hemagglutination. A supernatant and a sediment obtained after ultracentrifugation of LPS preparations possessed O-antigenic activity. O-specific polysaccharide (PS) is serologically less active than the LPS preparations. Problems of the intergroup and intragroup serological affinity in connection with the structure of O-specific PS. It is proved that the basic chain of O-specific polysaccharide (D-rhamnane) plays definite (but not a single) part in displaying antigenic properties of the whole LPS macromolecule.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 3","pages":"9-14"},"PeriodicalIF":0.0,"publicationDate":"1991-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12942759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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